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1.
Toxicon ; 57(7-8): 992-9, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21510970

RESUMO

Venomous fish are often involved in human accidents and symptoms of envenomation include local (intense pain and swelling) and systemic effects (cardiovascular and neurological disorders). However the only commercially available antivenom is against the Indo-Pacific stonefish Synanceja trachynisStonefish Antivenom (SFAV). The aim of the present study was to evaluate the potential of SFAV in neutralising the in vivo effects of some toxic activities of scorpionfish Scorpaena plumieri venom (SpV), and the in vitro immuno cross-reactivity. The SpV (7.5-100 µg/animal) caused nociceptive and dose-dependent edematogenic responses in the mice footpad. In rats SpV (300 µg/kg, i.v.) produced immediate and transient increase in arterial blood pressure and decrease in heart rate. Prior incubation of SpV with SFAV (1 µg SpV/1 U SFAV) abolished the inflammatory response, and significantly attenuated the cardiovascular effects induced by SPV. Western blotting analysis on two-dimensional SDS-PAGE of S plumieri venom proteins using SFAV proved that the epitopes recognized by SFAV are shared with the ∼98 kDa proteins. This is the first report of venom similarities between Indo-Pacific and Atlantic venomous fish, suggesting that the SpV compound responsible for inflammatory and cardiovascular effects possesses similar biochemical and antigenic properties to those found in stonefish venom.


Assuntos
Antivenenos/uso terapêutico , Doenças Cardiovasculares/tratamento farmacológico , Venenos de Peixe/antagonistas & inibidores , Peixes Venenosos , Inflamação/tratamento farmacológico , Animais , Antivenenos/química , Pressão Sanguínea/efeitos dos fármacos , Doenças Cardiovasculares/induzido quimicamente , Relação Dose-Resposta a Droga , Frequência Cardíaca/efeitos dos fármacos , Inflamação/induzido quimicamente , Masculino , Camundongos , Ratos , Ratos Wistar
2.
Arch Biochem Biophys ; 496(1): 9-20, 2010 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-20102699

RESUMO

We report the isolation and structure-function relationship of a 23kDa metalloproteinase named atroxlysin-I from the venom of the Peruvian Bothrops atrox (Jergón). Atroxlysin is a P-I metalloproteinase and contains 204 residues. Its proteolytic activity towards dimethylcasein is enhanced by Ca2+ but inhibited by EDTA, dithiothreitol, excessive Zn2+ and alpha2-macroglobulin. Unlike other structurally homologous P-I metalloproteinases, atroxlysin-I causes hemorrhages. To examine its hemorrhagic activity mechanistically, we studied its function in vitro and in vivo. It cleaved the Ala14-Leu15 and Tyr16-Leu17 bonds in oxidized insulin B-chain and specifically hydrolyzed the alpha-chains of fibrin(ogen) in a dose- and time-dependent manner. Atroxlysin-I cleaved plasma fibronectin and other extracellular matrix proteins (collagens I and IV) and the triple-helical fragment CB3 of collagen IV, but did not degrade laminin-111. Complementarily, the laminin and collagen binding integrins alpha7beta1 and alpha1beta1 were cleaved by atroxlysin. Even without catalytic activity atroxlysin-I inhibited collagen- and ADP-triggered platelet aggregation.


Assuntos
Plaquetas/efeitos dos fármacos , Vasos Sanguíneos/citologia , Bothrops , Matriz Extracelular/efeitos dos fármacos , Metaloproteases/toxicidade , Venenos de Serpentes/enzimologia , Sequência de Aminoácidos , Animais , Plaquetas/metabolismo , Vasos Sanguíneos/efeitos dos fármacos , Vasos Sanguíneos/metabolismo , Matriz Extracelular/metabolismo , Fibrina/metabolismo , Fibrinogênio/metabolismo , Fibronectinas/metabolismo , Hemorragia/induzido quimicamente , Hemostasia/efeitos dos fármacos , Humanos , Integrinas/metabolismo , Macroglobulinas/metabolismo , Metaloproteases/química , Metaloproteases/metabolismo , Dados de Sequência Molecular , Especificidade por Substrato
3.
Arch Biochem Biophys ; 468(2): 193-204, 2007 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-17963685

RESUMO

Leucurolysin-B (leuc-B) is an hemorrhagic metalloproteinase found in the venom of Bothrops leucurus (white-tailed-jararaca) snake. By means of liquid chromatography consisting of gel filtration on Sephracryl S-200, S-300 and ion-exchange on DEAE Sepharose, leuc-B was purified to homogeneity. The proteinase has an apparent molecular mass of 55kDa as revealed by the reduced SDS-PAGE, and represents approximately 1.2% of the total protein in B. leucurus venom. The partial amino acid sequence of leuc-B was determined by automated Edman sequencing of peptides derived from digests of the S-reduced and alkylated protein with trypsin. Leuc-B exhibits the characteristic motif of metalloproteinases, HEXXHXXGXXH and a methionine-containing turn of similar conformation ("Met-turn"), which forms a hydrophobic basis for the zinc ions and the three histidine residues involved as ligands. Leuc-B has been characterized as a P-III metalloproteinase and possesses a multidomain structure including a metalloproteinase, a disintegrin-like (ECD sequence instead of the typical RGD motif) and a cysteine-rich C-terminal domain. Leuc-B contains three potential sites of N-glycosylation. The enzyme only cleaves the Ala14-Leu15 peptide bond of the oxidized insulin B-chain and preferentially hydrolyzes the Aalpha-chain of fibrinogen and the alpha-chain of fibrin. Its proteolytic activity was completely inhibited by metal chelating agents but not by other typical proteinase inhibitors. In addition, its enzymatic activity was stimulated by the divalent cations Ca2+ and Mg2+ but inhibited by Zn2+ and Cu2+. The catalytic activity of leuc-B on extracellular matrix proteins could readily lead to loss of capillary integrity resulting in hemorrhage occurring at those sites (MHD=30ng in rabbit), with alterations in platelet function. In summary, here we report the isolation and the structure-function relationship of a P-III snake venom metalloproteinase.


Assuntos
Bothrops/metabolismo , Venenos de Crotalídeos/química , Metaloproteases/química , Metaloproteases/ultraestrutura , Sequência de Aminoácidos , Animais , Ativação Enzimática , Estabilidade Enzimática , Metaloproteases/classificação , Dados de Sequência Molecular
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