RESUMO
The sodA gene from Erwinia chrysanthemi strain 3937 was cloned by functional complementation of an Escherichia coli sodA sodB mutant and sequenced. We identified a 639-bp open reading frame, which encodes a protein that is 85% identical to the E. coli manganese-containing superoxide dismutase MnSOD. Promoter elements of this gene were identified by transcriptional mapping experiments. We constructed an E. chrysanthemi deltasodA mutant by reverse genetics. The deltasodA mutation resulted in the absence of a cytoplasmic SOD, which displays the same characteristics as those of MnSOD. The deltasodA mutant was more sensitive to paraquat than the wild-type strain. This mutant could macerate potato tubers, similar to the wild-type strain. In contrast, when inoculated on African violets, the mutant produced, at most, only small necrotic lesions. If the inoculum was supplemented with the superoxide anion-scavenging metalloporphyrin MnTMPyP or purified SOD and catalase, the deltasodA mutant was able to macerate the inoculated zone. Generation of superoxide anion by African violet leaves inoculated with E. chrysanthemi was demonstrated with nitroblue tetrazolium as an indicator. Therefore, at the onset of infection, E. chrysanthemi cells encounter an oxidative environment and require active protective systems against oxidative damages such as MnSOD to overcome these types of conditions.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/fisiologia , Dickeya chrysanthemi/enzimologia , Dickeya chrysanthemi/patogenicidade , Magnoliopsida/microbiologia , Doenças das Plantas/microbiologia , Superóxido Dismutase/genética , Superóxido Dismutase/fisiologia , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Clonagem Molecular , Citoplasma/enzimologia , Dickeya chrysanthemi/genética , Escherichia coli , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Dados de Sequência Molecular , Mutagênese Insercional , Doenças das Plantas/genética , Proteínas Recombinantes/biossíntese , Superóxido Dismutase/metabolismoRESUMO
Erwinia chrysanthemi causes soft-rot disease in a great variety of plants. In addition to the depolymerizing activity of plant cell wall-degrading enzymes, iron acquisition and resistance to oxidative stress contribute greatly to the virulence of this pathogen. Here, we studied the pin10 locus originally thought to encode new virulence factors. The sequence analysis revealed six open reading frames that were homologous to the Escherichia coli sufA, sufB, sufC, sufD, sufS and sufE genes. Sequence similarity searching predicted that (i) SufA, SufB, SufD, SufS and SufE proteins are involved in iron metabolism and possibly in Fe-S cluster assembly; and (ii) SufC is an ATPase of an ABC transporter. The reverse transcription-polymerase chain reaction procedure showed that the sufABCDSE genes constitute an operon. Expression of a sufB:uidA fusion was found to be induced in iron-deficient growth conditions and to be repressed by the iron-sensing Fur repressor. Each of the six suf genes was inactivated by the insertion of a cassette generating a non-polar mutation. The intracellular iron level in the sufA, sufB, sufC, sufS and sufE mutants was higher than in the wild type, as assessed by increased sensitivity to the iron-activated antibiotic streptonigrin. In addition, inactivation of sufC and sufD led to increased sensitivity to paraquat. Virulence tests showed that sufA and sufC mutants exhibited reduced ability to cause maceration of chicory leaves, whereas a functional sufC gene was necessary for the bacteria to cause systemic invasion of Saintpaulia ionantha. The E. coli sufC homologue was inactivated by reverse genetic. This mutation was found to modify the soxR-dependent induction of soxS gene expression. We discuss the possibility that SufC is a versatile ATPase that can associate either with the other Suf proteins to form a Fe-S cluster-assembling machinery or with membrane proteins encoded elsewhere in the chromosome to form an Fe-S ABC exporter. Overall, these results stress the importance of the connection between iron metabolism and oxidative stress during the early steps of infection by E. chrysanthemi.
Assuntos
Transportadores de Cassetes de Ligação de ATP/metabolismo , Adenosina Trifosfatases/fisiologia , Proteínas de Bactérias/metabolismo , Dickeya chrysanthemi/metabolismo , Proteínas de Escherichia coli , Estresse Oxidativo , Transativadores , Fatores de Transcrição/metabolismo , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/metabolismo , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Sequência de Bases , DNA Bacteriano , Dickeya chrysanthemi/genética , Dickeya chrysanthemi/patogenicidade , Genes Bacterianos , Homeostase , Ferro/metabolismo , Dados de Sequência Molecular , Óperon , Proteínas Repressoras/metabolismo , Análise de Sequência de DNA , Fatores de Transcrição/genética , VirulênciaRESUMO
The structure of a citrate siderophore named achromobactin isolated from the culture medium of Erwinia chrysanthemi was elucidated by spectroscopic methods and chemical degradation.
Assuntos
Citratos/química , Dickeya chrysanthemi/química , Ácidos Cetoglutáricos/química , Sideróforos/química , Citratos/análise , Citratos/isolamento & purificação , Dickeya chrysanthemi/crescimento & desenvolvimento , Ácidos Cetoglutáricos/isolamento & purificação , Modelos Moleculares , Conformação Molecular , Sideróforos/isolamento & purificaçãoRESUMO
Mutants of Erwinia amylovora CFBP 1430 lacking a functional high-affinity iron transport system mediated by desferrioxamine are impaired in their ability to initiate fire blight symptoms (A. Dellagi, M.-N. Brisset, J.-P. Paulin, and D. Expert. Mol. Plant-Microbe Interact. 11:734-742, 1998). In this study, a chromosomal transcriptional lacZ fusion was used to analyze the expression in planta of the E. amylovora ferrioxamine receptor gene foxR. LacZ activity produced by the strain harboring the fusion was highly induced in iron-restricted conditions and in inoculated apple leaf tissues. Microscopic observation revealed differential expression of this gene in relation to the localization and density of bacterial cells within the diseased tissue. Thus, the ability of bacterial cells to express their iron transport system in accordance with environmental conditions is likely important for disease evolution.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Erwinia/genética , Erwinia/patogenicidade , Genes de Plantas , Receptores de Superfície Celular , Fusão Gênica Artificial , Expressão Gênica , Óperon Lac , Dados de Sequência Molecular , Mutação , Doenças das Plantas/microbiologia , Plantas Geneticamente Modificadas , Virulência/genéticaRESUMO
Peptide methionine sulfoxide reductase (MsrA), which repairs oxidized proteins, is present in most living organisms, and the cognate structural gene belongs to the so-called minimum gene set [Mushegian, A. R. & Koonin, E. V., (1996) Proc. Natl. Acad. Sci. USA 93, 10268-10273]. In this work, we report that MsrA is required for full virulence of the plant pathogen Erwinia chrysanthemi. The following differences were observed between the wild-type and a MsrA- mutant: (i) the MsrA- mutant was more sensitive to oxidative stress; (ii) the MsrA- mutant was less motile on solid surface; (iii) the MsrA- mutant exhibited reduced virulence on chicory leaves; and (iv) no systemic invasion was observed when the MsrA- mutant was inoculated into whole Saintpaulia ionantha plants. These results suggest that plants respond to virulent pathogens by producing active oxygen species, and that enzymes repairing oxidative damage allow virulent pathogens to survive the host environment, thereby supporting the theory that active oxygen species play a key role in plant defense.
Assuntos
Dickeya chrysanthemi/genética , Dickeya chrysanthemi/patogenicidade , Genes de Plantas , Oxirredutases/genética , Oxirredutases/metabolismo , Sequência de Bases , Clonagem Molecular , Dickeya chrysanthemi/enzimologia , Escherichia coli , Metionina Sulfóxido Redutases , Dados de Sequência Molecular , Estresse Oxidativo , Oxirredutases/isolamento & purificação , Plantas/microbiologia , Reação em Cadeia da Polimerase , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Virulência/genéticaRESUMO
Low iron availability is a triggering signal for coordinated expression of the genes encoding pectate lyases PelB, PelC, PelD, and PelE, and chrysobactin iron transport functions, which are two main determinants of phytopathogenicity of the Erwinia chrysanthemi strain 3937. The possible implication of the ferric uptake regulation (Fur) protein in this process was investigated. The E. chrysanthemi fur gene was cloned by functional complementation of an Escherichia coli fur mutant and sequenced. The 444-bp open reading frame identified was found to code for a protein highly similar to the E. coli Fur regulator. An E. chrysanthemi fur null mutant was constructed by reverse genetics. This mutant showed altered growth capacity and reduced pathogenicity on African violets. In a fur background, transcriptional lacZ fusions to genes belonging to the E. chrysanthemi high affinity iron transport systems were constitutively expressed. Transcription of the pelA, pelD, and pelE genes was analyzed, using fusions to the uidA reporter gene. Iron availability and a fur mutation did not influence the expression of pelA. In the presence of iron, pelD and pelE transcription levels were higher in the fur mutant than in the parental strain. Furthermore, iron deficiency stimulated the expression of both fusions in the fur mutant. These findings indicate that, in E. chrysanthemi 3937, (i) Fur negatively controls iron transport and genes encoding PelD and PelE, and (ii) additional factor(s) mediate iron regulation of the pel genes.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Dickeya chrysanthemi/fisiologia , Regulação Bacteriana da Expressão Gênica , Ferro/metabolismo , Plantas/microbiologia , Polissacarídeo-Liases/genética , Regiões Promotoras Genéticas , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Transcrição Gênica , Sequência de Aminoácidos , Proteínas de Bactérias/química , Sequência de Bases , Clonagem Molecular , Sequência Consenso , Dickeya chrysanthemi/genética , Dickeya chrysanthemi/patogenicidade , Escherichia coli/genética , Teste de Complementação Genética , Dados de Sequência Molecular , Fases de Leitura Aberta , Doenças das Plantas/virologia , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Proteínas Repressoras/química , Alinhamento de Sequência , Homologia de Sequência do Ácido NucleicoRESUMO
The critical role of iron in plant host-parasite relationships has been elucidated in diseases as different as the soft rot and fire blight incited by Erwinia chrysanthemi and E. amylovora, respectively. As in animal infections, the role of iron and its ligands in the virulence of plant pathogens seems to be more subtle than might be expected, and is intimately related to the life cycle of the pathogen within its host. This review discusses how iron, because of its unique position in biological systems, controls the activities of these plant pathogens. Molecular studies illustrating the key question of iron acquisition and homeostasis during pathogenesis are described. The production of siderophores by pathogens not only represents a powerful strategy to acquire iron from host tissues but may also act as a protective agent against iron toxicity. The need of the host to bind and possibly sequester the metal during pathogenesis is another central issue. Possible modes of iron competition between plant host and pathogen are considered.
RESUMO
To investigate the role of iron in Erwinia amylovora pathogenicity, virulence properties of two mutants of strain CFBP 1430 isolated by insertional mutagenesis and affected in the iron transport pathway mediated by desferrioxamine (DFO) were analyzed. One mutation (dfoA::MudIIpR13) disrupts DFO biosynthesis. The present analysis shows that this mutation affects an open reading frame that belongs to a biosynthetic gene cluster and shares identity with the alcA gene required for synthesis of the siderophore alcaligin in Bordetella spp. A second mutation (foxR::MudIIpR13) affects the synthesis of the ferrioxamine receptor FoxR, encoded by the foxR gene, and was shown to be transcribed into a monocistronic message. Accordingly, the foxR mutant accumulates DFO in the external medium. The growth of the mutants when supplied with various iron sources was examined; it indicates that the production of DFO and the specific transport of the DFO ferric complex are required only when iron is strongly liganded. Pathogenicity was scored after inoculation of apple seedlings and after infection of apple flowers. On seedlings, the DFO biosynthetic mutant behaved like the wild-type strain while the frequency of necrotic plants caused by the receptor mutant decreased by a factor of two to five, depending on the initial inoculum. On flowers, both mutants were strongly affected in their ability to initiate a necrotic symptom and their growth was reduced by two orders of magnitude relative to the wild-type strain. However, the virulence of the dfoA mutant varied with the inoculum concentration. Unlike the foxR mutant, the dfoA mutant only weakly induced plant cell electrolyte leakage in tobacco leaf disks. The supply with exogenous DFO, only when iron free, restored the ability to induce electrolyte leakage to the dfoA mutant and increased the leakage induced by other strains. DFO alone was not an inducer. Iron-free DFO was able to protect E. amylovora cells against lethal doses of hydrogen peroxide. The main conclusion was that production of DFO in E. amylovora during pathogenesis is not only a critical function for iron acquisition, but can play a role in the oxidative burst elicited by the bacteria.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Desferroxamina/metabolismo , Erwinia/patogenicidade , Ferro/metabolismo , Receptores de Superfície Celular , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Sequência de Bases , Erwinia/genética , Peróxido de Hidrogênio/farmacologia , Dados de Sequência Molecular , Doenças das Plantas , Plantas Tóxicas , Rosales/microbiologia , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Nicotiana/microbiologia , Equilíbrio HidroeletrolíticoRESUMO
The main virulence factors of the phytopathogenic bacterium Erwinia chrysanthemi are pectinases that cleave pectin, a major constituent of the plant cell wall. Although physiological studies suggested that pectinase production in Erwinia species is subjected to catabolite repression, the direct implication of the cyclic AMP receptor protein (CRP) in this regulation has never been demonstrated. To investigate the role of CRP in pectin catabolism, we cloned the E. chrysanthemi crp gene by complementation of an Escherichia coli crp mutation and then constructed E. chrysanthemi crp mutants by reverse genetics. The carbohydrate fermentation phenotype of the E. chrysanthemi crp mutants is similar to that of an E. coli crp mutant. Furthermore, these mutants are unable to grow on pectin or polygalacturonate as the sole carbon source. Analysis of the nucleotide sequence of the E. chrysanthemi crp gene revealed the presence of a 630-bp open reading frame (ORF) that codes for a protein highly similar to the CRP of E. coli. Using a crp::uidA transcriptional fusion, we demonstrated that the E. chrysanthemi CRP represses its own expression, probably via a mechanism similar to that described for the E. coli crp gene. Moreover, in the E. chrysanthemi crp mutants, expression of pectinase genes (pemA, pelB, pelC, pelD, and pelE) and of genes of the intracellular part of the pectin degradation pathway (ogl, kduI, and kdgT), which are important for inducer formation and transport, is dramatically reduced in induced conditions. In contrast, expression of pelA, which encodes a pectate lyase important for E. chrysanthemi pathogenicity, seems to be negatively regulated by CRP. The E. chrysanthemi crp mutants have greatly decreased maceration capacity in potato tubers, chicory leaves, and celery petioles as well as highly diminished virulence on saintpaulia plants. These findings demonstrate that CRP plays a crucial role in expression of the pectinolysis genes and in the pathogenicity of E. chrysanthemi.
Assuntos
Dickeya chrysanthemi/genética , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Poligalacturonase/genética , Receptores de AMP Cíclico/genética , Sequência de Aminoácidos , Sequência de Bases , Escherichia coli/genética , Dados de Sequência Molecular , Mutação , Alinhamento de SequênciaRESUMO
Iron is unlikely to be readily available in plant tissues for invading microorganisms. Soft rot, caused by Erwinia chrysanthemi strain 3937 on African violets, is a valuable model for studying the role of iron and its ligands in plant-pathogen interactions. These studies could lead to the development of new control strategies against microbial infections of plants.
Assuntos
Ferro/metabolismo , Plantas/metabolismo , Plantas/microbiologia , Sequência de Carboidratos , Dickeya chrysanthemi/genética , Dickeya chrysanthemi/metabolismo , Dickeya chrysanthemi/patogenicidade , Homeostase , Dados de Sequência Molecular , Pectinas/química , Pectinas/metabolismo , Doenças das Plantas/microbiologia , Sideróforos/metabolismo , VirulênciaRESUMO
Iron deprivation of Erwinia amylovora CFBP1430, a species causing fire blight on Pomoïdeae, was shown to induce the production of siderophores of the desferrioxamine (dfo) family and two outer membrane polypeptides with apparent molecular weight of about 70 and 80 kDa, respectively. Cyclic dfo E was characterized as the major metabolite. Phage MudIIpR13 insertional mutagenesis and screening on CAS-agar medium yielded three dfo non-producing and one overproducing clones. These clones failed to grow in the presence of the Fe(III) chelator EDDHA and were determined further as dfo and ferrioxamine transport negative mutants, respectively. The transport mutant which appeared to lack the 70 kDa polypeptide in the outer membrane allowed the purification of dfo E. Growth under iron limitation of dfo negative mutants was stimulated with ferrioxamine E and B but not with other ferrisiderophores tested. The host DNA sequence flanking the left terminal part of the MudIIpR13 prophage responsible for the transport mutation was cloned and used to probe a parental gene library by DNA-DNA hybridization. Two recombinant cosmids restoring the transport mutation to normal were identified. Both cosmids also conferred the ability to utilize ferrioxamine B and E as iron sources on a FhuE- mutant of Escherichia coli. This correlated with the production of an additional polypeptide of 70 kDa in the outer membrane of E. coli transconjugants, thus confirming that this protein serves the ferrioxamine receptor function (FoxR) in E. amylovora.
Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Desferroxamina/farmacologia , Erwinia/metabolismo , Proteínas de Escherichia coli , Genes Bacterianos , Ferro/metabolismo , Receptores de Superfície Celular , Proteínas da Membrana Bacteriana Externa/biossíntese , Proteínas da Membrana Bacteriana Externa/genética , Transporte Biológico/efeitos dos fármacos , Clonagem Molecular , Conjugação Genética , Erwinia/efeitos dos fármacos , Erwinia/genética , Escherichia coli/metabolismo , Peso Molecular , Mutagênese Insercional , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , Mapeamento por RestriçãoRESUMO
The fct cbsCEBA operon from the Erwinia chrysanthemi 3937 chrysobactin-dependent iron assimilation system codes for transport and biosynthetic functions. The sequence of the fct outer membrane receptor gene was determined. The fct promoter region displays a strong resemblance to the Escherichia coli bidirectional intercistronic region controlling the expression of the fepA-entD and fes-entF operons. An apparent Fur-binding site was shown to confer iron regulation on an fct::lac fusion expressed on a low-copy-number plasmid in a Fur-proficient E. coli strain. The fct gene consists of an open reading frame encoding a 735-amino-acid polypeptide with a signal sequence of 38 residues. The Fct protein has 36% sequence homology with the E. coli ferrichrome receptor FhuA and the Yersinia enterocolitica ferrioxamine receptor FoxA. On the basis of secondary-structure predictions and these homologies, we propose a two-dimensional folding model for Fct.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Dickeya chrysanthemi/genética , Proteínas de Escherichia coli , Genes Bacterianos , Receptores de Superfície Celular/genética , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Hidrolases de Éster Carboxílico/genética , Proteínas de Transporte/genética , Dipeptídeos/metabolismo , Compostos Férricos/metabolismo , Regulação Bacteriana da Expressão Gênica , Ferro/metabolismo , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Óperon , Regiões Promotoras Genéticas , Estrutura Secundária de Proteína , Receptores Virais/genética , Proteínas Repressoras/metabolismo , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Sideróforos/metabolismo , Transcrição GênicaRESUMO
In planta expression of a high-affinity iron-uptake system involving the siderophore chrysobactin in Erwinia chrysanthemi 3937 contributes greatly to invasive growth of this pathogen on its natural host, African violets. A previous study reported that global regulation by iron in this strain was mediated at the transcriptional level via the cbr locus which, when inactivated by insertional mutation, prevents the chrysobactin system from being tightly repressed by FeCl3. Herein, we report the nucleotide sequence of this locus and the functional analysis of its encoded products. Sequence analysis of a 4.8 kb genomic segment of a plasmid encompassing the cbr locus and characterization of the cognate translated products made it possible to uncover a system exhibiting similarity with prokaryotic transporters implicated in the transport of iron complexes. Accordingly, the CbrA product was shown to be the periplasmic component of a permease complex also including two integral membrane proteins, CbrB and CbrC, and the ATP-binding unit CbrD. This system allowed internalization of Fe(III) when supplied to bacterial cells as 59FeCl3 or 59Fe dicitrate, via complexation to a second siderophore recently detected in strain 3937. Most notably, we demonstrate that this second siderophore-mediated iron-acquisition system is operational in bacterial cells grown in the presence of FeCl3. The regulatory effect of cbr was further assessed on a lacZ chrysobactin operon fusion indicating that the transcriptional control exerted by cbr on expression of the chrysobactin system is of homeostatic nature. in conclusion, E. chrysanthemi provides an interesting model in which iron acquisition involves an inductive process resulting in differential expression of two siderophore-mediated pathways in relation to external iron accessibility.
Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Dickeya chrysanthemi/genética , Genes Bacterianos , Ferro/metabolismo , Sideróforos/metabolismo , Transportadores de Cassetes de Ligação de ATP/metabolismo , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/genética , Sequência de Bases , Transporte Biológico , Compartimento Celular , Dickeya chrysanthemi/metabolismo , Dipeptídeos/genética , Dipeptídeos/metabolismo , Compostos Férricos/metabolismo , Expressão Gênica , Proteínas de Membrana/biossíntese , Proteínas de Membrana/genética , Dados de Sequência Molecular , Fases de Leitura Aberta , Proteínas Recombinantes de Fusão/biossíntese , Mapeamento por Restrição , Análise de Sequência de DNARESUMO
In this communication, we examine the fate of iron during soft rot pathogenesis caused by Erwinia chrysanthemi on its host, Saintpaulia ionantha. The spread of soft rot caused by this enterobacterium was previously shown to depend on a functional genetic locus encoding a high-affinity iron assimilation system involving the catechol-type siderophore chrysobactin. Leaf intercellular fluid from healthy plants was analyzed with regard to the iron content and its availability for bacterial growth. It was compared to the fluid from diseased plants for the presence of strong iron ligands, using a new approach based on the iron-binding property of an ion-exchange resin. Further characterization allowed the identification of chrysobactin in diseased tissues, thus providing the first evidence for the external release of a microbial siderophore during pathogenesis. Competition for nutritional iron was also studied through a plant-bacterial cell system: iron incorporated into plant ferritin appeared to be considerably reduced in bacteria-treated suspension soybean cells. The same effect was visualized during treatment of soybean cells with axenic leaf intercellular fluid from E. chrysanthemi-inoculated saintpaulia leaves or with chrysobactin.
RESUMO
Erwinia chrysanthemi 3937 possesses a saturable, high-affinity transport system for the ferric complex of its native siderophore chrysobactin, [N-alpha-(2,3-dihydroxybenzoyl)-D-lysyl-L-serine]. Uptake of 55Fe-labeled chrysobactin was completely inhibited by respiratory poison or low temperature and was significantly reduced in rich medium. The kinetics of chrysobactin-mediated iron transport were determined to have apparent Km and Vmax values of about 30 nM and of 90 pmol/mg.min, respectively. Isomers of chrysobactin and analogs with progressively shorter side chains mediated ferric iron transport as efficiently as the native siderophore, which indicates that the chrysobactin receptor primarily recognizes the catechol-iron center. Free ligand in excess only moderately reduced the accumulation of 55Fe. Chrysobactin may therefore be regarded as a true siderophore for E. chrysanthemi.
Assuntos
Catecóis/metabolismo , Dickeya chrysanthemi/metabolismo , Dipeptídeos/metabolismo , Compostos Férricos/metabolismo , Temperatura Baixa , Cinética , Espectroscopia de Ressonância MagnéticaRESUMO
Systemic virulence of the phytopathogen Erwinia chrysanthemi 3937 requires a functional iron assimilation system which, in this enterobacterium, is mediated by the siderophore chrysobactin and the outer membrane transport protein Fct. We investigated the regulation of this system by iron. No direct similarity with the Escherichia coli fur gene was found. Insertional mutagenesis allowed isolation of a regulatory mutant which expressed chrysobactin and two other high-affinity iron transport systems previously characterized in strain 3937, regardless of the iron level. RNA/DNA hybridization analysis established that regulation of chrysobactin by iron occurs at the transcriptional level. From a wild-type gene library, a recombinant cosmid able to restore normal regulation in the mutant strain was isolated. By generating a series of subclones and mini-Mulac insertions, we identified a regulatory locus (cbr) extending beyond c. 2.5kb which encodes two polypeptides, CbrA and CbrB, with molecular weights of 34,000 and 55,000 respectively. Functional analysis of the locus suggests that the cognate genes cbrA and cbrB are clustered within an operon. Their expression was studied through chromosomal lac gene fusions, in the presence of plasmid-borne wild-type constructions, under high- and low-iron conditions. In summary, the data show that in the presence of iron, cbr negatively regulates the chrysobactin biosynthetic and transport genes, while under conditions of depletion, cbr is subject to negative autogeneous regulation.
Assuntos
Dickeya chrysanthemi/genética , Genes Bacterianos/genética , Ferro/metabolismo , Transporte Biológico/fisiologia , Clonagem Molecular , Dickeya chrysanthemi/metabolismo , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica/fisiologia , Genes Bacterianos/fisiologia , Ferro/fisiologia , Mutação , Transcrição Gênica/fisiologiaRESUMO
We isolated a monoclonal antibody directed against the ferric complex of aerobactin purified from Escherichia coli KH576. This antibody, which we designated MAb AERO1, was identified as an immunoglobulin G, subtype 2. A competitive enzyme-linked immunosorbent assay with MAb AERO1 had a limit of 10 nM for the detection of purified ferric aerobactin and allowed detection of the crude aerobactin produced by various members of the family Enterobacteriaceae isolated from cancer patients with bacteremia. The only two other structurally related siderophores recognized by MAb AERO1 were ferric arthrobactin and ferrioxamine B. These results suggest that the epitope recognized by MAb AERO1 was the lysyl moiety of ferric aerobactin. We also showed that MAb AERO1 reduced the growth of an aerobactin-producing strain of E. coli in newborn calf serum, which indicates that it might be effective in reducing the severity of infections caused by bacteria for which the production of aerobactin is an important virulence factor.
Assuntos
Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Enterobacteriaceae/química , Ácidos Hidroxâmicos/análise , Animais , Anticorpos Monoclonais/biossíntese , Ensaio de Imunoadsorção Enzimática , Ácidos Hidroxâmicos/imunologia , Ácidos Hidroxâmicos/isolamento & purificação , Camundongos , Camundongos Endogâmicos BALB CRESUMO
The iron assimilation system of Erwinia chrysanthemi 3937 is mediated by the catechol-type siderophore chrysobactin and the outer membrane transport protein Fct. We generated a variety of subclones in high- and low-copy-number vectors from a wild-type recombinant cosmid shown previously to carry the gene cluster fct-cbsA, cbsB, cbsC, cbsE encoding chrysobactin transport and biosynthetic functions, respectively. We studied their expression in Escherichia coli enterobactin-deficient entA, entB, entC, and entE mutants. This provided evidence that the fct and cbs genes are regrouped within a single genetic unit of ca. 8 kb in the following order: fct, cbsC, cbsE, cbsB, and cbsA. The gene boundaries were determined, and the various recombinant plasmids were expressed in Escherichia coli minicells: CbsA and CbsC enzymatic activities were clearly identified as polypeptides with apparent molecular masses of 32,000 and 38,000, respectively.
Assuntos
Dickeya chrysanthemi/genética , Dipeptídeos/metabolismo , Ferro/metabolismo , Óperon , Transporte Biológico , Mapeamento Cromossômico , Clonagem Molecular , Dickeya chrysanthemi/metabolismo , Dickeya chrysanthemi/patogenicidade , Eletroforese em Gel de Poliacrilamida , Escherichia coli/genética , Família Multigênica , Receptores de Superfície Celular/genética , Mapeamento por Restrição , Virulência/genéticaRESUMO
In vitro studies of bacteriophage Mu transposition have shown that the phage-encoded transposase and repressor bind the same sequences on the phage genome. We attempted to test that prediction in vivo and found that Mu repressor directly inhibits transposition. We also found that, in the absence of repressor, constitutive expression of Mu transposition functions pA and pB is lethal in Escherichia coli strains lysogenic for a mini-Mu and that this is the result of intensive replication of the mini-Mu. These findings have important consequences where such mini-Mus are used as genetic tools. We also tested whether in Erwinia chrysanthemi the effect of transposition functions on a resident mini-Mu was the same as in E. coli. We observed that expression of pA alone was lethal in E. chrysanthemi and that a large fraction of the survivors underwent precise excision of the mini-Mu.