Rapid determination of total flavonoid content, xanthine oxidase inhibitory activities, and antioxidant activity in Prunus mume by near-infrared spectroscopy.

Evaluating the quality of herbal medicine based on the content and activity of its main components is highly beneficial. Developing an eco-friendly determination method has significant application potential. In this study, we propose a new method to simultaneously predict the total flavonoid content (TFC), xanthine oxidase inhibitory (XO) activity, and antioxidant activity (AA) of Prunus mume using near-infrared spectroscopy (NIR). Using the sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method, uric acid colorimetric method, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) free radical scavenging activity as reference methods, we analyzed TFC, XO, and AA in 90 P. mume samples collected from different locations in China. The solid samples were subjected to NIR. By employing spectral preprocessing and optimizing spectral bands, we established a rapid prediction model for TFC, XO, and AA using partial least squares regression (PLS). To improve the model's performance and eliminate irrelevant variables, competitive adaptive reweighted sampling (CARS) was used to calculate the pretreated full spectrum. Evaluation model indicators included the root mean square error of cross-validation (RMSECV) and determination coefficient (R2) values. The TFC, XO, and AA model, combining optimal spectral preprocessing and spectral bands, had RMSECV values of 0.139, 0.117, and 0.121, with RCV2 values exceeding 0.92. The root mean square error of prediction (RMSEP) for the TFC, XO, and AA model on the prediction set was 0.301, 0.213, and 0.149, with determination coefficient (RP2) values of 0.915, 0.933, and 0.926. The results showed a strong correlation between NIR with TFC, XO, and AA in P. mume. Therefore, the established model was effective, suitable for the rapid quantification of TFC, XO, and AA. The prediction method is simple and rapid, and can be extended to the study of medicinal plant content and activity.

Differentiation of Polygonatum Cyrtonema Hua from Different Geographical Origins by near-Infrared Spectroscopy with Chemometrics.

BACKGROUND: The identification of the geographical origin of Polygonatum cyrtonema Hua is of particular importance because the quality and market value of Polygonatum cyrtonema Hua from different production areas are highly variable due to differences in the growing environment and climatic conditions. OBJECTIVE: This study utilized near-infrared spectra (NIR) of Polygonatum cyrtonema Hua (n = 400) to develop qualitative models for effective differentiation of Polygonatum cyrtonema Hua from various regions. METHODS: The models were produced under different conditions to distinguish the origins distinctly. Ten pre-processing methods have been used to pre-process the original spectra (OS) and to select the most optimal spectral pre-processing method. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were employed to determine appropriate models. For simplicity, the pretreated full spectrum was calculated by different wavelength selection methods, and the four most significant variables were selected as discriminant indicator variables. RESULTS: The results show that Polygonatum cyrtonema Hua from different regions can be effectively distinguished using spectra from a series of samples analyzed by OPLS-DA. The accuracy of the OPLS-DA model is also satisfactory, with a good differentiation rate. CONCLUSION: The study findings indicate the feasibility of using spectroscopy in combination with multivariate analysis to identify the geographical origins of Polygonatum cyrtonema Hua. HIGHLIGHTS: The utilization of near-infrared spectroscopy combined with chemometrics exhibits high efficacy in discerning the provenance of herbal medicines and foods, thereby facilitating quality assurance measures.

Progress in the study of intestinal microbiota involved in morphine tolerance.

Morphine is a widely used opioid for treatment of pain. The attendant problems including morphine tolerance and morphine dependence pose a major public health challenge. In recent years, there has been increasing interest in the gastrointestinal microbiota in many physiological and pathophysiological processes. The connectivity network between the gut microbiota and the brain is involved in multiple biological systems, and bidirectional communication between them is critical in gastrointestinal tract homeostasis, the central nervous system, and the microbial system. Many research have previously shown that morphine has a variety of effects on the gastrointestinal tract, but none have determined the function of intestinal microbiota in morphine tolerance. This study reviewed the mechanisms of morphine tolerance from the perspective of dysregulation of microbiota-gut-brain axis homeostasis, by summarizing the possible mechanisms originating from the gut that may affect morphine tolerance and the improvement of morphine tolerance through the gut microbiota.

Combining QSAR techniques, molecular docking, and molecular dynamics simulations to explore anti-tumor inhibitors targeting Focal Adhesion Kinase.

Focal Adhesion Kinase (FAK) is an important target for tumor therapy and is closely related to tumor cell genesis and progression. In this paper, we selected 46 FAK inhibitors with anticancer activity in the pyrrolo pyrimidine backbone to establish 3D/2D-QSAR models to explore the relationship between inhibitory activity and molecular structure. We have established two ideal models, namely, the Topomer CoMFA model (q2= 0.715, r2= 0.984) and the Holographic Quantitative Structure-Activity Relationship (HQSAR) model (q2= 0.707, r2= 0.899). Both models demonstrate excellent external prediction capabilities.Based on the QSAR results, we designed 20 structurally modified novel compounds, which were subjected to molecular docking and molecular dynamics studies, and the results showed that the new compounds formed many robust interactions with residues within the active pocket and could maintain stable binding to the receptor proteins. This study not only provides a powerful screening tool for designing novel FAK inhibitors, but also presents a series of novel FAK inhibitors with high micromolar activity that can be used for further characterization. It provides a reference for addressing the shortcomings of drug metabolism and drug resistance of traditional FAK inhibitors, as well as the development of novel clinically applicable FAK inhibitors.Communicated by Ramaswamy H. Sarma.

Blaming the unvaccinated during the COVID-19 pandemic: the roles of political ideology and risk perceptions in the USA.

Individuals unvaccinated against COVID-19 (C19) experienced prejudice and blame for the pandemic. Because people vastly overestimate C19 risks, we examined whether these negative judgements could be partially understood as a form of scapegoating (ie, blaming a group unfairly for an undesirable outcome) and whether political ideology (previously shown to shape risk perceptions in the USA) moderates scapegoating of the unvaccinated. We grounded our analyses in scapegoating literature and risk perception during C19. We obtained support for our speculations through two vignette-based studies conducted in the USA in early 2022. We varied the risk profiles (age, prior infection, comorbidities) and vaccination statuses of vignette characters (eg, vaccinated, vaccinated without recent boosters, unvaccinated, unvaccinated-recovered), while keeping all other information constant. We observed that people hold the unvaccinated (vs vaccinated) more responsible for negative pandemic outcomes and that political ideology moderated these effects: liberals (vs conservatives) were more likely to scapegoat the unvaccinated (vs vaccinated), even when presented with information challenging the culpability of the unvaccinated known at the time of data collection (eg, natural immunity, availability of vaccines, time since last vaccination). These findings support a scapegoating explanation for a specific group-based prejudice that emerged during the C19 pandemic. We encourage medical ethicists to examine the negative consequences of significant C19 risk overestimation among the public. The public needs accurate information about health issues. That may involve combating misinformation that overestimates and underestimates disease risk with similar vigilance to error.

The Gut Microbiota-Brain Axis: Potential Mechanism of Drug Addiction.

As a chronic encephalopathy, drug addiction is responsible for millions of deaths per year around the world. The gut microbiome is a crucial component of the human microbiome. Through dynamic bidirectional communication along the 'gut-brain axis,' gut bacteria cooperate with their hosts to regulate the development and function of the immune, metabolic, and nervous systems. These processes may affect human health because some brain diseases are related to the composition of gut bacteria, and disruptions in microbial communities have been implicated in neurological disorders. We review the compositional and functional diversity of the gut microbiome in drug addiction. We discuss intricate and crucial connections between the gut microbiota and the brain involving multiple biological systems and possible contributions by the gut microbiota to neurological disorders. Finally, the treatment of probiotics and fecal transplantation was summarized. This was done to further understand the role of intestinal microecology in the pathogenesis of drug addiction and to explore new methods for the treatment of drug addiction.

Dynamics of multipartite quantum steering for different types of decoherence channels.

Multipartite quantum steering, a unique resource for asymmetric quantum network information tasks, is very fragile to the inevitable decoherence, which makes it useless for practical purposes. It is thus of importance to understand how it decays in the presence of noise channels. We study the dynamic behaviors of genuine tripartite steering, reduced bipartite steering, and collective steering of a generalized three-qubit W state when only one qubit interacts independently with the amplitude damping channel (ADC), phase damping channel (PDC) or depolarizing channel (DC). Our results provide the region of decoherence strength and state parameters that each type of steering can survive. The results show that these steering correlations decay the slowest in PDC and some non-maximally entangled states more robust than the maximally entangled ones. Unlike entanglement and Bell nonlocality, the thresholds of decoherence strength that reduced bipartite steering and collective steering can survive depend on the steering direction. In addition, we find that not only one party can be steered by a group system, but also two parties can be steered by a single system. There is a trade-off between the monogamy relation involving one steered party and two steered parties. Our work provides comprehensive information about the effect of decoherence on multipartite quantum steering, which will help to realize quantum information processing tasks in the presence of noise environments.

When Do Observers Deprioritize Due Process for the Perpetrator and Prioritize Safety for the Victim in Response to Information-Poor Allegations of Harm?

We examined how observers assess information-poor allegations of harm (e.g., "my word against yours" cases), in which the outcomes of procedurally fair investigations may favor the alleged perpetrator because the evidentiary standards are unmet. Yet this lack of evidence does not mean no harm occurred, and some observers may be charged with deciding whether the allegation is actionable within a collective. On the basis of theories of moral typecasting, procedural justice, and uncertainty management, we hypothesized that observers would be more likely to prioritize the victim's safety (vs. to prioritize due process for the perpetrator) and view the allegation as actionable when the victim-alleged perpetrator dyad members exhibit features that align with stereotypes of victims and perpetrators. We supported our hypothesis with four studies using various contexts, sources of perceived prototypicality, due-process prioritization, and samples (students from New Zealand, Ns = 137 and 114; Mechanical Turk workers from the United States; Ns = 260 and 336).

Case Report: Surgical management of idiopathic pulmonary aneurysms and review surgical approaches.

Idiopathic pulmonary aneurysm is a clinically rare condition characterized by an unknown etiology and episodic occurrence. Despite its rarity, idiopathic pulmonary artery aneurysm poses potential risks to patients. Currently, there is a lack of established clinical guidelines and consensus regarding its management, leading to ongoing controversies in treatment strategies. Particularly, the optimal approach for addressing the main pulmonary artery, its branches, and the pulmonary artery valve remains uncertain. A 57-year-old female patient presented with chest pain and tightness, leading to the diagnosis of idiopathic pulmonary artery aneurysm after excluding other potential causes. Subsequently, she underwent surgical treatment. However, during the surgery, the pulmonary artery wall was found to be extremely weak, prompting us to employ a surgical approach involving the utilization of autologous vessel wrapping with artificial grafts. By summarizing almost all surgical treatment strategies reported in recent years, including the management of pulmonary artery vessels and the pulmonary valve, we have developed a treatment flow chart. This flowchart serves as a valuable guide for the management of future cases presenting similar challenges, offering clinicians valuable insights and evidence-based recommendations.

Demonstration of universal contextuality through communication games free of both operational inequivalence and compatibility loopholes.

Universal contextuality is the leading notion of non-classicality even for single systems, showing its advantage as a more general quantum correlation than Bell non-locality, as well as preparation contextuality. However, a loophole-free experimental demonstration of universal contextuality at least requires that both operational inequivalence and compatibility loopholes are closed, which have never been simultaneously achieved to date. In our work, we experimentally test universal contextuality through (3,3) and (4,3) communication games, simultaneously restoring operational equivalence and circumventing the compatibility loophole. Our result exhibits the violation of universal non-contextuality bound by 97 standard deviations in (3,3) scenario, and 107 deviations in (4,3) scenario. Notably there are states which exhibit locality but reveal universal contextuality in both two scenarios. In addition, our result shows that universal contextuality is more general than preparation contextuality in (3,3) scenario, while equivalent to preparation contextuality in (4,3) scenario.

Protecting nonlocal quantum correlations in correlated squeezed generalized amplitude damping channel.

Nonlocal quantum correlations, such as quantum entanglement, quantum steering, and Bell nonlocality, are crucial resources for quantum information tasks. How to protect these quantum resources from decoherence is one of the most urgent problems to be solved. Here, we investigate the evolution of these correlations in the correlated squeezed generalized amplitude damping (SGAD) channel and propose a scheme to protect them with weak measurement (WM) and quantum measurement reversal (QMR). Compared with the results of the uncorrelated SGAD channel, we find that when [Formula: see text], correlation and squeezing effects can prolong the survival time of quantum entanglement, Bell nonlocality, and quantum steering by about 152 times, 207 times, and 10 times, respectively. In addition, local WM and QMR can effectively recover the disappeared nonlocal quantum correlations either in uncorrelated or completely correlated SGAD channels. Moreover, we find that these initial nonlocal quantum correlations could be drastically amplified under the correlated channel. And the steering direction can be flexibly manipulated either by changing the channel parameters or the strength of WM and QMR. These results not only make a step forward in suppressing decoherence and enhancing quantum correlation in noise channels, but also help to develop relevant practical applications.

Investigation of the influence of measurement imperfections on quantum communication complexity superiority for the Clauser-Horne-Shimony-Holt game.

Demonstrating quantum communication complexity superiority non-trivially with currently available experimental systems is of utmost importance in quantum information science. Here, we propose a generalized entanglement-assisted communication complexity reduction protocol and analyze the robustness of its quantum superiority against the measurement imperfections, such as measurement basis deviation and choice probability bias, a common problem rarely studied before. We find that the quantum superiority can be obtained in a specific entangled state in a suitable range of measurement basis and basis choice parameters. And the quantum superiority strengthens with the increase of the entanglement degree of quantum states. By using the maximum entangled state and its corresponding optimal measurement, the result we obtained violated the optimal classical bound by 239 standard deviations. Besides, the robustness of effective measurement basis in dephasing and depolarizing quantum channels is also investigated. These results not only make a step forward in investigating sufficient experimental conditions to unambiguously demonstrate the superiority of quantum communication complexity but also help to develop relevant practical applications.

Precise portrayal of microscopic processes of wastewater biofilm formation: Taking SiO2 as the model carrier.

Precise characterization of the microscopic processes of wastewater biofilm formation is essential for regulating biofilm behavior. Nevertheless, it remains a great challenge. This study investigated biofilm formation on SiO2 carriers under gradually increasing shear force combining the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory in a Couette-Taylor reactor, and precisely revealed the micro-interface interaction and species colonization during biofilm formation. The results indicated that bacterial reversible adhesion distance on SiO2 carrier surface was 3.06 ± 0.48 nm. Meanwhile, the secondary minimum of total XDLVO interaction energy could be used as a novel indicator to distinguish biofilm formation stages. The revealed biofilm formation stages were also confirmed by the electrochemical analysis. Additionally, the pioneer species that colonized at first were Comamonadaceae, Azospira, Flavobacterium and Azonexus, while keystone species such as Hydrogenophaga, AKYH767, Aquimonas and Ignavibacterium determined the stability of microbial community. In conclusion, this study provided a methodological example to study wastewater biofilm micro-interface behavior through the integration of an experimental platform as well as multiple monitoring and analysis methods, which opened up new perspectives for biofilm research and provided useful guidance for the regulation of biofilm-related treatment processes and new technology development.

The dark side of belief in Covid-19 scientists and scientific evidence.

We draw from an interdisciplinary literature on convictions to examine the manifestations and consequences of firmly held beliefs in Covid-19 (C19) science. Across three studies (N = 743), we assess participants' beliefs in C19 experts, and beliefs in supported and unsupported empirical evidence. Study 1 establishes the basic theoretical links and we show that an individual's belief in science on C19 is associated with dispositional belief in science and moralization of C19 mitigation measures. Our subsequent two studies show how stronger belief in C19 science influences distrust in unmasked individuals past the mandates, and greater endorsement of pandemic mitigation authoritarianism. We document the dark side that emerges when belief in C19 science extends beyond the generally desirable scientific literacy and manifests as a conviction that public health experts are the only ones who can handle the pandemic, and that even unsupported claims about C19 are supported by scientific evidence (e.g., risk of outdoor transmission is high). We also highlight our political ideology findings showing that both liberals and conservatives mis-calibrate C19 risks in different ways, and we conclude with discussing how examining the darker side of scientific beliefs can inform our understanding of people's reactions to the pandemic.

Harsh but Expedient: Dominant Leaders Increase Group Cooperation via Threat of Punishment.

Dominant leadership is, surprisingly, on the rise globally. Previous studies have found that intergroup conflict increases followers' support for dominant leaders, but identifying the potential benefits that such leaders can supply is crucial to explaining their rise. We took a behavioral-economics approach in Study 1 (N = 288 adults), finding that cooperation among followers increases under leaders with a dominant reputation. This pattern held regardless of whether dominant leaders were assigned to groups, elected through a bidding process, or leading under intergroup competition. Moreover, Studies 2a to 2e (N = 1,022 adults) show that impressions of leader dominance evoked by personality profiles, authoritarian attitudes, or physical formidability similarly increase follower cooperation. We found a weaker but nonsignificant trend when dominance was cued by facial masculinity and no evidence when dominance was cued by aggressive disposition in a decision game. These findings highlight the unexpected benefits that dominant leaders can bestow on group cooperation through threat of punishment.

Antibacterial efficacy of poly(hexamethylene biguanide) immobilized on chitosan/dye-modified nanofiber membranes.

This study aimed to develop a novel electrospun polyacrylonitrile (PAN) nanofiber membrane with the enhanced antibacterial property. The PAN nanofiber membrane was first subjected to alkaline hydrolysis treatment, and the treated membrane was subsequently grafted with chitosan (CS) to obtain a CS-modified nanofiber membrane (P-COOH-CS). The modified membrane was then coupled with different dye molecules to form P-COOH-CS-Dye membranes. Lastly, poly(hexamethylene biguanide) hydrochloride (PHMB) was immobilized on the modified membrane to produce P-COOH-CS-Dye-PHMB. Physical characterization studies were conducted on all the synthesized nanofiber membranes. The antibacterial efficacies of nanofiber membranes prepared under different synthesis conditions were evaluated systematically. Under the optimum synthesis conditions, P-COOH-CS-Dye-PHMB was highly effective in disinfecting a high concentration of Escherichia coli, with an antibacterial efficacy of approximately 100%. Additionally, the P-COOH-CS-Dye-PHMB exhibited an outstanding wash durability as its antibacterial efficacy was only reduced in the range of 5%-7% even after 5 repeated cycles of treatment. Overall, the experimental results of this study suggested that the P-COOH-CS-Dye-PHMB is a promising antibacterial nanofiber membrane that can be adopted in the food, pharmaceutical, and textile industries.

Microscopic analysis towards rhamnolipid-mediated adhesion of Thiobacillus denitrificans: A QCM-D study.

Rhamnolipid was proved to increase the abundance of Thiobacillus denitrificans in the mixotrophic denitrification biofilm while its microscopic mechanism remains to be explored. Effect of rhamnolipids on deposition of macromolecular substances and adhesion of Thiobacillus denitrificans at room (20 °C) and low temperature (10 °C) were systematically investigated by the quartz crystal microbalance with dissipation monitoring (QCM-D) for the first time. Results showed that low concentration of rhamnolipids (20-80 mg/L) could promote the deposition of macromolecular substances by reducing hydraulic repulsion force, with the maximum deposition amount increased by 4.28 times than that of the control at room temperature. Deposition amount of microorganisms could be improved by increasing its concentration at room temperature while it didn't work at low temperature. Meanwhile, low temperature could significantly inhibit adhesion of Thiobacillus denitrificans (p < 0.05) and deposited layers under low concentration of rhamnolipids were generally rigid, resulting in the negative feedback effect on the microorganisms' adhesion. While high concentration of rhamnolipids (120-200 mg/L) could regulate the biofilm from rigid to viscoelastic and significantly promote the initial adhesion of Thiobacillus denitrificans on SiO2 surface (p < 0.05). This study demonstrated the microscopic mechanism of rhamnolipids on the initial biofilm formation, that is, the reduction of hydration repulsion force was responsible for the enhanced deposition of macromolecules while the regulation of biofilm properties was account for the promoted adhesion of Thiobacillus denitrificans.

Culture, assumptions about the world, and interpretations of children's disabilities.

Sim et al. (2021) examined the interplay between parental caretakers and children with health disabilities in East Asian cultures. Their analyses suggested that the way East Asian mothers responded to their disabled children may have to do with the culture in which they were embedded. Complementing their work, we aim to integrate their findings with the cultural psychology literature, focusing on styles of thought and supernatural beliefs. Doing so allows us to forge theoretical links between Sim et al. (2021) and frameworks that delineate the distinct ways of thought in East Asian cultures, recommend promising directions for future research, and motivate interdisciplinary readership.

Revisiting the Microscopic Processes of Biofilm Formation on Organic Carriers: A Study under Variational Shear Stresses.

The microscopic process of biofilm development on carriers is critical for interfacial regulation of biofilms in attached-growth wastewater treatment. However, the process under shear stress has not been well understood. The study purposed to revisit the processes of biofilm formation on organic carriers under different shear stresses with special highlights on bacterial reversible adhesion and pioneers in the microbial community. Biofilm formation on high-density polyethylene, polyamide, acrylonitrile butadiene styrene plastic, polyvinyl chloride, and polycarbonate carriers under shear stresses ranging from 1.0 to 2.5 Pa was investigated using Couette-Taylor reactors. Employing extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory, the bacterial reversible adhesion regions ranging from 3.74 ± 0.20 to 5.51 ± 0.24 nm on an organic carrier were quantified for the first time, elucidating significant differences among different carriers (p < 0.01). The colonization of pioneers in the microbial community was significantly altered by shear stress rather than carrier properties (p < 0.01). In particular, the diversity of the biofilm microbial community was pronouncedly enhanced by a higher shear stress (p < 0.01). XDLVO analysis suggested that extracellular polymeric substances had a negative feedback on subsequent microbial adhesion and biofilm development, especially the transition from reversible to irreversible bacterial adhesion. This study contributed to a better understanding of the biofilm formation process at the microscopic scale and shed light on micro-interfacial manipulation for biofilm accumulation or renewal.

Moralization of Covid-19 health response: Asymmetry in tolerance for human costs.

We hypothesized that because Covid-19 (C19) remains an urgent and visible threat, efforts to combat its negative health consequences have become moralized. This moralization of health-based efforts may generate asymmetries in judgement, whereby harmful by-products of those efforts (i.e., instrumental harm) are perceived as more acceptable than harm resulting from non-C19 efforts, such as prioritizing the economy or non-C19 issues. We tested our predictions in two experimental studies. In Study 1, American participants evaluated the same costs (public shaming, deaths and illnesses, and police abuse of power) as more acceptable when they resulted from efforts to minimize C19's health impacts, than when they resulted from non-health C19 efforts (e.g., prioritizing economic costs) or efforts unrelated to C19 (e.g., reducing traffic deaths). In Study 2, New Zealand participants less favorably evaluated the quality of a research proposal empirically questioning continuing a C19 elimination strategy in NZ than one questioning abandoning an elimination strategy, although both proposals contained the same amount of methodology information. This finding suggests questioning elimination approaches is morally condemned, a similar response to that found when sacred values are questioned. In both studies, condition effects were mediated by lowered moral outrage in response to costs resulting from pursuing health-minded C19 efforts. Follow-up analyses revealed that both heightened personal concern over contracting C19 and liberal ideology were associated with greater asymmetries in human cost evaluation. Altogether, results suggest efforts to reduce or eliminate C19 have become moralized, generating asymmetries in evaluations of human suffering.