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1.
Dev Comp Immunol ; 145: 104730, 2023 08.
Artigo em Inglês | MEDLINE | ID: mdl-37169042

RESUMO

We compared the secretion of antimicrobial peptides (AMPs) in the epidermal mucus of three healthy fish species: two aquacultured teleost species, Dicentrarchus labrax and Sparus aurata, and one wild-caught species, Pagrus pagrus. The AMPs detected in all mucus by LC-MS/MS-QTOF are: Chrysophsin-1, -2 and -3, Piscidins -1, -2, -3 and -4, terminal Histone parts and Hepcidin-like peptides. Secretion analysis of the mucus from aquacultured fish using ProGenesis IQ software distinguished the bactericidal activities of histone peptides and probiotic flora from those of other AMPs. Chrysophsin-1 was statistically the most abundant peptide in both mucus samples (p < 0.0035). A lower detection of Piscidins was also observed. Interestingly, the presence of Oncorhyncin I was most pronounced in Sparus aurata mucus. Altogether, these results suggest that Chrysophsin-1 and Oncorhyncin I are potential biomarkers for immunodetection-based studies of changes in secretion patterns which will be further investigated during bacterial challenge.


Assuntos
Proteoma , Dourada , Animais , Proteoma/metabolismo , Peptídeos Antimicrobianos , Cromatografia Líquida/métodos , Histonas , Espectrometria de Massas em Tandem , Dourada/metabolismo , Muco/metabolismo
2.
Fish Shellfish Immunol ; 35(2): 207-12, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23643872

RESUMO

Antibacterial protection in the mucus is provided by antimicrobial compounds and till now few numbers of AMP and proteins were identified. Herein, mass spectral profiling of fresh mucus from farmed sea bass (Dicentrarchus labrax) using Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF) and liquid chromatography mass spectrometry is investigated in order to survey the infective/healthy status of the mucus. We identify AMP peptides of 2891.7, 2919.45 and 2286.6 Da molecular weight respectively and characterize Chrysophsins in the mucus of Dicentrarchus labrax. These peptides display broad-spectrum bactericidal activity against Gram-negative (Minimum Inhibitory Concentrations namely MICs < 0.5 µM) and Gram-positive bacteria (MICs < 0.5 µM) including Escherichia coli and Bacillus subtilis. Furthermore, sensitivity to yeast Candida albicans is reported for the first time and shows interesting MICs of less than 2 µM. We also demonstrate that the fish pathogen Aeromonas salmonoicida is sensitive to Chrysophsins (MICs ranging between 5 and 14 µM). Our mucus molecular mass mapping developed approach allows for fast exploration of immune status. Our data provides evidence that Chrysophsins are secreted by immune cells and are released in mucus of non-challenged farmed European sea bass. These results suggest that Chrysophsins, secreted by gills of red sea bream, are an important widespread component of Teleostei defense against disease.


Assuntos
Peptídeos Catiônicos Antimicrobianos/imunologia , Bass/imunologia , Cromatografia Líquida/métodos , Proteínas de Peixes/imunologia , Proteoma/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Animais , Peptídeos Catiônicos Antimicrobianos/análise , Aquicultura , Candida albicans , Cromatografia Líquida/veterinária , Proteínas de Peixes/análise , Bactérias Gram-Negativas , Bactérias Gram-Positivas , Imunidade Inata , Testes de Sensibilidade Microbiana/veterinária , Muco/química , Muco/imunologia , Espectrometria de Massas por Ionização por Electrospray/veterinária , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/veterinária , Tunísia
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