RESUMO
The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 æg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 æg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production
Assuntos
Aflatoxina B1 , Aflatoxinas , Aspergillus flavus , Flavonoides , Extratos Vegetais , Análise de Variância , Aspergillus flavus , Flavonoides , Extratos VegetaisRESUMO
The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 microg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 microg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production.
Assuntos
Aflatoxina B1/biossíntese , Aflatoxinas/biossíntese , Aspergillus flavus/efeitos dos fármacos , Flavonoides/farmacologia , Extratos Vegetais/farmacologia , Análise de Variância , Aspergillus flavus/metabolismo , Flavonoides/química , Extratos Vegetais/químicaRESUMO
Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 micrograms/ml and 500 micrograms/ml. respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 micrograms/ml. Virucidal activity was not observed at 62.5 micrograms/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 micrograms/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.
Assuntos
Antivirais/farmacologia , Aphthovirus/efeitos dos fármacos , Herpesvirus Bovino 1/efeitos dos fármacos , Herpesvirus Suídeo 1/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Bovinos , Linhagem Celular/efeitos dos fármacos , Células Cultivadas , Testes de Sensibilidade Microbiana , Extratos Vegetais/toxicidade , SuínosRESUMO
Crude extracts of leaves and fruits of Guarea guidona were tested antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine Cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 mug/ml and 500 mug/ml, respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 mug/ml. Virucidal activity was not observed at 62.5 mug/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 mug/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.
Assuntos
Animais , Bovinos , Antivirais/farmacologia , Aphthovirus/efeitos dos fármacos , Herpesvirus Bovino 1/efeitos dos fármacos , Herpesvirus Suídeo 1/efeitos dos fármacos , Extratos Vegetais/farmacologia , Linhagem Celular/efeitos dos fármacos , Células Cultivadas , Testes de Sensibilidade Microbiana , Extratos Vegetais/toxicidade , SuínosRESUMO
Two biflavonoids were isolated from the EtOH extract from leaves of Ouratea spectabilis. Their structures were established to be the novel 6,6"-bigenkwanin and 7,7"-dimethoxyagathisflavone on the basis of spectroscopic data. The inhibition of bovine lens aldose reductase exerted by these biflavones has been studied.