Characteristics of aerosol optical depth dynamics and their causes over typical cities along the 21st Century Maritime Silk Road.

Atmospheric aerosols, i.e., suspension of liquid and/or solid particles in air, have serious impacts on human health. Exploring the variation and patterns of regional atmospheric aerosols is of great significance to monitor and evaluate atmosphere quality, especially in urban areas with large population. Here, with nine typical pivotal cities along the 21st Century Maritime Silk Road through Southeast Asia, South Asia to West Asia as case studies, based on MCD19A2 550 nm AOD products, combined meteorological factors, land use data, and nighttime light data, we analyzed the spatio-temporal distribution, variation features, influencing and/or driving factors of aerosols in developed urban areas over Asia. The results showed that the descending sequence of the annual aerosol optical depth (AOD) of the nine cities was Karachi, Doha, Chittagong, Bangkok, Colombo, Ho Chi Minh, Singapore, Gwadar, and Yangon during 2013-2018. Due to the influence of regional climate system and atmospheric aerosol types, the time series of annual, seasonal, and monthly AODs were significantly different. The high values of AODs in most cities were mainly located in the urban center or rapid socio-economic (e.g., industrial and agricultural) development regions. The effects of different meteorological factors on the AODs varied in different cities. The rainfall, relative humidity, and wind speed had great impacts on AODs in Ho Chi Minh, Bangkok, Singapore, and Yangon. Temperature, relative humidity, and wind speed had close correlations with AODs in Chittagong, Colombo, Karachi, and Gwadar of South Asia and Doha in West Asia. The urban area's AOD was influenced by the combined and synergistic effects of socio-economy, urbanization, and meteorological factors, with that in Karachi being the most significant.

Effect of a DNA vaccine harboring two copies of inhibin α (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats.

The objective was to investigate the effects of a novel DNA vaccine (pcISI) harboring two copies of inhibin α (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats. Female Wistar rats (n=18 per group) were immunized (twice, 4 wk apart) with 10, 50, or 100 µg (T1, T2 and T3, respectively), of the pcISI plasmid. At 4 wk after the second immunization, plasma antibody titers were higher (P<0.05) in T3 than in either T1 or T2 (0.341±0.123, 0.236±0.068, and 0.251±0.077, respectively, mean±SD). Concurrrently, plasma concentrations of FSH and estradiol were highest (P<0.05) in T3, and were higher (P<0.05) in T1 and T2 than in control groups. For antibody-positive rats, there was a correlation (P<0.01) between antibody titer and FSH concentrations after two pcISI immunizations. The number of mature follicles in the T3 group (46.00±4.65) was higher (P<0.05) than in two control groups (29.25±3.72 and 27.92±3.48), and also higher (P<0.05) than in T1 and T2 (37.17±4.99 and 38.75±7.09). Antibody-positive rats had more mature ovarian follicles than negative rats (46.75±4.23 vs. 35.60±3.38, P<0.05). Moreover, litter size and number of placentas were increased (P<0.05) in the pcISI immunization groups, except for the T1 group, compared to the control groups. In conclusion, the pcISI DNA vaccine successfully induced a humoral immune response, improved reproductive hormone concentrations, stimulated follicular development, and increased number of placentas and litter size. Furthermore, 100 µg yielded the best immune response.

[Influence of meliorated AS-PCR primer for ABO genotyping].

OBJECTIVE: To improve the correct rate of ABO genotyping by meliorating AS-PCR primer. METHODS: The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed. RESULTS: The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer. CONCLUSION: The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.