Ferrocene-Labelled Electroactive Aptamer-Based Sensors (Aptasensors) for Glycated Haemoglobin.

Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3' and 5'-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c.

A supramolecular pyrenyl glycoside-coated 2D MoS2 composite electrode for selective cell capture.

Here we demonstrate the simple construction and characterization of a pyrenyl glycoside-coated 2D MoS2 material composite capable of selectively capturing proteins and live cells on an electrode, as determined by differential pulse voltammetry.

Probing Mannose-Binding Proteins That Express on Live Cells and Pathogens with a Diffusion-to-Surface Ratiometric Graphene Electrosensor.

This paper describes the development of a "diffusion-to-surface" ratiometric graphene electrosensor for the selective detection of live cells and pathogens that highly express mannose-binding proteins (MBPs). MBPs have been implicated in many pathological processes and are identified on specific types of bacteria. Consequently, MBPs are a promising biomarker for targeted disease diagnosis and therapy. Here, we develop a unique electrosensor that features a ratiometric voltammetric signal for the selective probing of MBPs. Self-assembly of mannosyl anthraquinone (AQ) to a graphene oxide-decorated screen-printed electrode produces the sensor with an inherent surface-controlled voltammetric signal. Subsequently, addition of a redox probe (RP) imparts the system with a diffusion-controlled current, thus enabling a ratiometric sensing rationale for which AQ serves as a reference. While the reference current is hardly compromised by adding analytes, RP exhibits a concentration-dependent current quenching on addition of mannose-selective lectins over other proteins. Importantly, this ratiometric electrosensor has proven to be applicable for the ratiometric probing of alternatively activated macrophages and a Gram-negative bacterium highly expressing MBPs, but shows minimal response to a series of control live cells and bacteria without mannose receptor expression.