RESUMO
This study evaluates the long-term outcome of farmer's lung (FL), adding high-resolution computed tomograms (HRCT) to previously reported procedures and verifying whether bronchoalveolar lavage (BAL) fluid markers or substrates of fibrosis (hyaluronic acid, Type III procollagen, fibronectin, and fibroblast growth factors) (FF) predict outcome. A total of 33 subjects with a history of FL dating back at least 6 yr were evaluated with pulmonary function tests, chest x-ray (CXR), and HRCT. All subjects had an initial evaluation, which included a BAL, 6 yr before the current study. Subjects were then either in acute FL (n = 19) or in clinical remission despite continued contact (n = 14). In the current study, pulmonary function tests revealed an obstructive profile in 13 subjects, restrictive changes in 1, an isolated decrease in lung diffusion capacity in 3, and normal values in 16. Chest radiographs (CXR) were normal in 22 subjects, abnormal with interstitial or reticulonodular changes in 6, and suggestive of emphysema in 5. HRCT revealed emphysema in 9 subjects; 3 had localized fibrotic changes, 2 a ground-glass pattern, and 19 were normal. There was a good correlation between the findings on pulmonary function tests and HRCT; however, CXR alone did not suggest the existence of emphysema in 4 subjects who had such findings on HRCT. No correlations were found between most outcome parameters and the level of the BAL FF measured 6 yr previously. We conclude that airflow obstruction with or without emphysema is an important long-term sequela of FL and that BAL FF do not predict outcome in this disease.
Assuntos
Líquido da Lavagem Broncoalveolar/citologia , Pulmão de Fazendeiro/diagnóstico , Doença Aguda , Adulto , Idoso , Líquido da Lavagem Broncoalveolar/química , Pulmão de Fazendeiro/complicações , Pulmão de Fazendeiro/epidemiologia , Pulmão de Fazendeiro/terapia , Feminino , Humanos , Análise dos Mínimos Quadrados , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Prognóstico , Indução de Remissão , Testes de Função Respiratória/estatística & dados numéricos , Fatores de Tempo , Tomografia Computadorizada por Raios X , Resultado do TratamentoRESUMO
The authors report a patient with a fibrovascular polyp of the esophagus. Radiologic and pathological features of this rare tumor include computed tomographic findings of a lesion of low density, situated intraluminally so that the findings may be relatively specific.
Assuntos
Doenças do Esôfago/diagnóstico por imagem , Fibroma/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adulto , Doenças do Esôfago/patologia , Doenças do Esôfago/cirurgia , Fibroma/patologia , Fibroma/cirurgia , Humanos , MasculinoRESUMO
DNA synthesis, mitotic activity and cell migration have been measured in organ culture of adult mouse jejunum during the first 48 hr. Explants cultured in DMEM-HEPES-NCTC-135 medium present a sharp decrease of mitotic activity in the first hours of culture, but mitoses are restored to 80% of the controls between 24 and 48 hr. DNA synthesis follows the same pattern. Cell migration continues during culture.
Assuntos
Duodeno/citologia , Mucosa Intestinal/citologia , Jejuno/citologia , Mitose , Técnicas de Cultura de Órgãos , Animais , Movimento Celular , Meios de Cultura , DNA/biossíntese , Duodeno/metabolismo , Mucosa Intestinal/metabolismo , Jejuno/metabolismo , Masculino , CamundongosRESUMO
Explants of adult mouse intestine have been maintained in organ culture for 24 to 48 hr. The best results have been obtained with a mixture of DMEM-HEPES medium and NCTC-135 enriched with 10% fetal bovine serum. The morphology of the mucosa is well preserved at the light and electron microscopic level: absorbing cells exhibit an increase in secondary lysosomes; goblet cells and Paneth cells remain active; numerous mitoses are observed in the crypts; and vigorous re-epithelization takes place on the margin of the explants.