Honeybees generalize among pollen scents from plants flowering in the same seasonal period.

When honey bees (Apis mellifera) feed on flowers, they extend their proboscis to absorb the nectar, i.e. they perform the proboscis extension response (PER). The presence of pollen and/or nectar can be associated with odors, colors or visual patterns, which allows honey bees to recognize food sources in the environment. Honey bees can associate similar, though different, stimuli with the presence of food; i.e. honey bees discriminate and generalize among stimuli. Here, we evaluated generalization among pollen scents from six different plant species. Experiments were based on the PER conditioning protocol over two phases: (1) conditioning, in which honey bees associated the scent of each pollen type with sucrose, and (2) test, in which honey bees were presented with a novel scent, to evaluate generalization. Generalization was evinced by honey bees extending their proboscis to a novel scent. The level of PER increased over the course of the conditioning phase for all pollen scents. Honey bees generalized pollen from Pyracantha coccinea and from Hypochaeris radicata These two plants have different amounts of protein and are not taxonomically related. We observed that the flowering period influences the olfactory perceptual similarity and we suggest that both pollen types may share volatile compounds that play key roles in perception. Our results highlight the importance of analyzing the implications of the generalization between pollen types of different nutritional quality. Such studies could provide valuable information for beekeepers and agricultural producers, as the generalization of a higher quality pollen can benefit hive development, and increase pollination and honey production.

Effect of Vegetable Oils as Phagostimulants in Adults of Dichroplus vittigerum (Orthoptera: Acrididae).

Several species of grasshoppers are attracted to vegetable oils. These oils have kairomonal properties mainly due to the presence of linolenic and linoleic fatty acids. This study aimed to determine whether the odors of canola, flax, and olive oils are attractive to Dichroplus vittigerum (Blanchard 1851) and if they induce preference and feeding. We conducted three bioassays to determine whether oil modifies attraction and feeding behavior of this grasshopper. We first determined the attraction of the oils using a wind tunnel, secondly evaluated phagostimulation produced by the oils, and finally performed preference tests comparing consumption of Taraxacum officinale (Weber ex F.H.Wigg. 1780, Asterales: Asteraceae) leaves treated with the oils versus control leaves. Even though all of the oils induced attraction, only flax oil acted as a phagostimulant. However, the oils did not determine the preference and did not increase feeding on leaves. We hypothesize that T. officinale leaves are inherently attractive and treatment with oils did not affect feeding on them. Our results provide a starting point to develop baits that can be used to attract and control these harmful insects, presenting flax oil as a potential bait for D. vittigerum since its odor was both attractive and led to increased feeding or phagostimulation. Future studies should test the effect of the oils on other plant species or at varying doses, under field conditions.

Cumulative effects of volcanic ash on the food preferences of two Orthopteran species.

Inert dusts are an early form of insecticide which is still in use. One of the most common inert dusts is volcanic ash. In order to study the reaction of rangeland grasshoppers, Dichroplus vittigerum (Acrididae) and a katydid, Burgilis mendosensis (Phaneropteridae), to the presence of volcanic ash in their food sources and how this reaction changed as a function of time, we conducted paired preference tests between clean leaves of their preferred host plant and leaves exposed to volcanic ash of different grain size. The behavioral response was measured as the rating on the Thurstonian preference scale of leaves with ash in relation to clean leaves. The results showed that the avoidance of volcanic ash increased as a function of time in both species. Both species studied are occasionally exposed to volcanic activity, and come from an area in which a volcanic eruption had recently occurred. As their populations did not decrease after the ash fall, we propose that some behavioral responses such as avoidance of places with ash, works as tolerance mechanism to inert dusts exposure.

Neo-sex Chromosomes in the Maculipennis Species Group (Dichroplus: Acrididae, Melanoplinae): The Cases of D. maculipennis and D. vittigerum.

South American melanopline grasshoppers display a disproportionate number of derived karyotypes, including many cases of neo-sex chromosome systems. This is especially true of the genus Dichroplus and its Maculipennis species group. We analyzed the karyotype and neo-sex chromosomes in mitosis and meiosis of Dichroplus maculipennis and D. vittigerum from Argentina using conventional and fluorescent cytogenetic protocols in order to elucidate the behavior and origin of these neo-XY systems in relation to the current phylogeny of this group. Our results showed that D. maculipennis (2n = 22♂/22♀; neoXY/neoXX) and D. vittigerum, whose karyotype is described here for the first time (2n = 18♂/18♀; neoXY/neoXX), show highly evolved neo-XY systems, although with significant differences between them. Furthermore, both species differ for two autosomal fixed Robertsonian fusions present in D. vittigerum. Analysis of karyotypic character state optimization strongly suggests the independent origin and evolution of neo-sex systems within this species group.