Discurso dos enfermeiros da atenção básica acerca das práticas educativas aos adolescentes / Advice of nurses of basic attention on educational practices for teenagers / Discurso de los enfermeros de la atención básica acerca de las prácticas educativas a los adolescentes

Objetivos: analisar os discursos dos enfermeiros da Atenção Básica acerca das práticas educativas voltadas para os adolescentes. Método: estudo descritivo com abordagem qualitativa realizado com enfermeiros das Unidades Básicas de saúde da cidade de Cajazeiras. Os dados foram obtidos através de entrevistas semiestruturadas e analisadas por meio da Análise do Discurso. Resultados: os enfermeiros discursaram a impregnação de ações voltadas para sexualidade e drogas, com métodos verticalizados, no entanto começa a emergir a necessidade de utilizar novos recursos metodológicos, capazes de desenvolver o interesse dos jovens, e observar o contexto social ao qual o sujeito está inserido, com o objetivo de educar não para controlar, mas educar para libertar. Conclusões: as metodologias utilizadas pelos enfermeiros na educação em saúde para os adolescentes ainda estão fincadas na utilização de palestras, deste modo torna-se necessário novas pesquisas de cunho intervencionista que apontem uma orientação adequada para práticas educativas direcionadas aos adolescentes

Objective: The study's main purpose has been to analyze the primary care nurses' statements concerning the educational activities for adolescents. Methods: This descriptive study with a qualitative approach was performed in basic health units from the municipality of Cajazeiras, Paraíba State, Brazil. Data were obtained through semi-structured interviews and submitted to Discourse Analysis. Results: The registered nurses reported the incorporation of actions directed to sexuality and drugs with the use of verticalized methods. Nonetheless, the need to use new methodological resources capable of attracting young people's interest, and considering their social context begins to emerge. The purpose of education is not to control people but liberate them. Conclusion: The health education methodologies used by nurses to work with adolescents are still limited to lectures. Hence, further research, especially using an interventionist approach, needs to be conducted. Such research might help to provide adequate guidance on how to perform education activities targeted at adolescents

Objetivo: analizar los discursos de los enfermeros de la Atención Básica acerca de las prácticas educativas dirigidas a los adolescentes. Método: estudio descriptivo con abordaje cualitativo realizado con enfermeros de las Unidades Básicas de salud de la ciudad de Cajazeiras. Los datos fueron obtenidos a través de entrevistas semiestructuradas y analizadas por medio del Análisis del Discurso. Resultados: los enfermeros discursaron la impregnación de acciones dirigidas a la sexualidad y las drogas, con métodos verticalizados, sin embargo comienza a emerger la necesidad de utilizar nuevos recursos metodológicos, capaces de desarrollar el interés de los jóvenes, y observar el contexto social al que el sujeto está inserto, con el objetivo de educar no para controlar, sino educar para liberar. Consideraciones finales: las metodologías utilizadas por los enfermeros en la educación en salud para los adolescentes todavía están clavadas en la utilización de conferencias, de este modo se hacen necesarias nuevas investigaciones de cuño intervencionista que apunte una orientación adecuada para prácticas educativas dirigidas a los adolescentes

Molecular interactions of human Exo1 with DNA.

Human Exo1 is a member of the RAD2 nuclease family with roles in replication, repair and recombination. Despite sharing significant amino acid sequence homology, the RAD2 proteins exhibit disparate nuclease properties and biological functions. In order to identify elements that dictate substrate selectivity within the RAD2 family, we sought to identify residues key to Exo1 nuclease activity and to characterize the molecular details of the human Exo1-DNA interaction. Site-specific mutagenesis studies demonstrate that amino acids D78, D173 and D225 are critical for Exo1 nuclease function. In addition, we show that the chemical nature of the 5'-terminus has a major impact on Exo1 nuclease efficiency, with a 5'-phosphate group stimulating degradation 10-fold and a 5'-biotin inhibiting degradation 10-fold (relative to a 5'-hydroxyl moiety). An abasic lesion located within a substrate DNA strand impedes Exo1 nucleolytic degradation, and a 5'-terminal abasic residue reduces nuclease efficiency 2-fold. Hydroxyl radical footprinting indicates that Exo1 binds predominantly along the minor groove of flap DNA, downstream of the junction. As will be discussed, our results favor the notion that the single-stranded DNA structure is pinched by the helical arch of the protein and not threaded through this key recognition loop. Furthermore, our studies indicate that significant, presumably biologically relevant, differences exist between the active site dynamics of Exo1 and Fen1.