Molecular Profile of Sensitization to Dermatophagoides pteronyssinus Dust Mite in Portugal.

BACKGROUND AND OBJECTIVES: To analyze component-resolved diagnosis of sensitization to Dermatophagoides pteronyssinus (Der p) in patients with respiratory allergy and the association between diagnostic findings and clinical severity in different geographical areas. METHODS: The study population comprised 217 patients (mean age, 25.85 [12.7] years; 51.16% female) selected from 13 centers in Portugal (5 from the North, n=65). All had allergic rhinitis with or without asthma and positive skin prick test results to at least 1 dust mite. Specific IgE (sIgE) to Der p, Dermatophagoides farinae, Lepidoglyphus destructor, Der p 1, Der p 2, Der p 10, and Der p 23 was determined using ImmunoCAP. The Mann-Whitney test was applied for the following comparisons: rhinitis vs rhinitis and asthma; mild vs moderate-to-severe rhinitis; North vs South. RESULTS: The prevalence of sensitization was 98.2% for Der p, and 72.4%, 89.4%, 9.7%, and 77% for Der p 1, Der p 2, Der p 10, and Der p 23, respectively. The corresponding median sIgE levels were 8.56, 17.7, 0.01, and 3.95 kUA/L. sIgE to all allergens was higher in patients with moderate-to-severe rhinitis and rhinitis with asthma (nonsignficant). Concentrations of sIgE to Der p 2 were significantly higher in the South than in the North (P=.0496). CONCLUSION: The most common sensitization in Portugal was to Der p. The highest prevalence and median sIgE level were observed for Der p 2. All sIgE values for molecular components were higher in more symptomatic patients (nonsignificant). Concentrations of sIgE to Der p 2 were higher in the South, probably because of the warmer temperature and/or the larger sample size.

The effect of heparin on cell proliferation and type-I collagen synthesis by adult human dermal fibroblasts.

(1), A commercial heparin inhibited the proliferation of two normal human dermal fibroblast cultures in a dose-responsive manner. 300 micrograms heparin/ml gave the maximum inhibition of 65%. Proliferation of fibroblasts from a patient with progressive systemic sclerosis was similarly inhibited by heparin. Heparan sulphate, pentosan polysulphate and a fucoidan also inhibited proliferation of the fibroblast cultures but chondroitin sulphate, dermatan sulphate and hyaluronate had no effect. (2) Type-I collagen synthesis per cell was elevated by up to 1.5-fold in the normal fibroblast cultures when proliferation was inhibited by heparin, heparan sulphate, pentosan polysulphate and the fucoidan. Progressive systemic sclerosis fibroblasts synthesized more collagen than the normal cell cultures and this was further stimulated by heparin and pentosan polysulphate. In contrast, heparin and the other polysulphates inhibited type-I collagen synthesis by about 20-30% in normal confluent fibroblast cultures. Collagen synthesis by confluent systemic sclerosis fibroblasts was reduced by only about 10%. (3), Total [35S]proteoglycan synthesis per cell was greatly elevated (approx. 2.5-fold) in normal subconfluent cultures treated with heparin. In contrast, heparin stimulated only a small increase in [35S]sulphate incorporation into proteoglycans in confluent cultures.