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Introdução: doenças Cardiovasculares são as principais causas de morte no mundo. Apesar da redução da incidência e mortalidade por DCV no século XX, os valores permanecem elevados no século XXI. No Brasil, há lacuna de estudos populacionais que estimaram as taxas padronizadas de mortalidade por doenças cardiovasculares em adultos jovens.Objetivo: avaliar a tendência da mortalidade por doenças cardiovasculares em adultos jovens, segundo sexo, faixa etária e regiões do Brasil.Método: estudo de séries temporais com uso de dados secundários oficiais dos Sistemas de Informações sobre Mortalidade (SIM). Foram consideradas todas as mortes por doenças cardiovasculares (I00-I-99) em adultos jovens faixa etária 20-49 anos, residentes no Brasil, no período de 01 de janeiro de 2008 a 31 de dezembro de 2017. Os dados foram extraídos do Departamento de Informática do SUS (DATASUS). Foi utilizado o modelo de regressão Prais-Winsten e calculada a Variação Percentual Anual (VPA). Todas as análises foram realizadas no software STATA 14.0.Resultados: durante período 2008-2017, foram identificadas 294.232 mortes (8,7%) por doença cardiovascular em adultos jovens com idade entre 20-49 anos. Identificou-se a redução da mortalidade por DCV em todas as regiões do Brasil, exceto nos indivíduos de 20-24 anos, residentes na região Nordeste, a qual apresentou aumento (VPA: 2,45%) (p<0,05) 2013-2017. A maior variação da tendência de mortalidade ocorreu na região Sul (VPA: -25,2%). Enquanto a menor variação de tendência da mortalidade ocorreu na região Nordeste (VPA: -8,8%). O declínio anual foi menor no segundo quinquênio (2013-2017) em comparação ao primeiro (2008-2012). Além disso, o declínio foi mais acentuado entre as mulheres (VPA: -2,51%) (p<0,05) 2008-2012 e em adultos jovens com idade entre 40-44 anos (VPA: -2,91%) (p<0,05) 2008-2012. Ademais, a tendência de mortalidade por DCV se estabilizou a partir de 2013 no sexo masculino (p>0,05).Conclusão: os resultados demonstram tendência decrescente da mortalidade por Doença Cardiovascular em adultos jovens no Brasil, entre 2008-2017. Conclui-se que existe desigualdade na tendência de mortalidade por DCV segundo sexo, faixa etária e regiões do Brasil.
Introduction: cardiovascular diseases are the leading causes of death in the world. Despite the reduction in CVD incidence and mortality in the 20th century, the values remain high in the 21st century. In Brazil, there is a gap in population studies that estimated standardized mortality rates from cardiovascular diseases in young adults.Objective: to assess the trend in mortality from cardiovascular diseases in young adults, according to sex, age group and regions of Brazil.Methods: ecological time series study using official secondary data from Mortality Information Systems (SIM). All deaths from cardiovascular diseases (I00-I-99) in young adults aged 20-49 years, residing in Brazil, in the period from January 1, 2008 to December 31, 2017, were considered. Data were extracted from the Department of Informatics of the SUS (DATASUS). The Prais-Winsten regression model was used and the Annual Percentage Variation (APV) was calculated. All analyzes were performed in STATA 14.0 software.Results: during the period 2008-2017, 294,232 deaths (8.7%) from cardiovascular disease were identified in young adults aged 20-49 years. A reduction in CVD mortality was identified in all regions of Brazil, except for individuals aged 20-24 years, residing in the Northeast region, which showed an increase (APC: 2.45%) (p<0.05) 2013 -2017. The greatest variation in the mortality trend occurred in the South region (APC: -25.2%). While the smallest change in mortality trend occurred in the Northeast region (APC: -8.8%). The annual decline was smaller in the second quinquennium (2013-2017) compared to the first (2008-2012). Furthermore, the decline was more pronounced among women (APC: -2.51%) (p<0.05) 2008-2012 and in young adults aged 40-44 years (APC: -2.91%) (p<0.05) 2008-2012. Furthermore, the trend in CVD mortality stabilized from 2013 onwards in males (p>0.05).Conclusion: the results demonstrate a decreasing trend in mortality from Cardiovascular Disease in young adults in Brazil, between 2008-2017. It is concluded that there is inequality in the trend of mortality from CVD according to sex, age group and regions of Brazil.
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Calotropis procera produces a milky sap containing proteolytic enzymes. At low concentrations, they induce milk-clotting (60 µg/ml) and to dehair hides (0.05 and 0.1%). A protocol for obtaining the enzymes is reported. The latex was mixed with distilled water and the mixture was cleaned through centrifugation. It was dialyzed with distilled water and centrifuged again to recover the soluble fraction [EP]. The dialyze is a key feature of the process. EP was characterized in terms of protein profile, chemical stability, among other criteria. Wild plants belonging to ten geographic regions and grown in different ecological conditions were used as latex source. Collections were carried out, spaced at three-month, according to the seasons at the site of the study. Proteolytic activity was measured as an internal marker and for determining stability of the samples. EP was also analyzed for metal content and microbiology. EP showed similar magnitude of proteolysis, chromatographic and electrophoretic profiles of proteins. Samples stored at 25 °C exhibited reduced solubility (11%) and proteolytic capacity (11%) after six months. Enzyme autolysis was negligible. Microbiological and metal analyses revealed standard quality of all the samples tested. EP induced milk clotting and hide dehairing after storage for up to six months.
Assuntos
Ácido Aspártico Endopeptidases/metabolismo , Calotropis/enzimologia , Técnicas de Química Analítica/normas , Ecossistema , Látex/química , Proteínas de Plantas/metabolismo , Estações do Ano , Pelo Animal/efeitos dos fármacos , Animais , Ácido Aspártico Endopeptidases/análise , Ácido Aspártico Endopeptidases/química , Ácido Aspártico Endopeptidases/farmacologia , Bovinos , Cabras , Remoção de Cabelo/métodos , Látex/isolamento & purificação , Proteínas de Plantas/análise , Proteínas de Plantas/química , Proteínas de Plantas/farmacologia , Proteólise , Padrões de Referência , SolubilidadeRESUMO
The present work intends to study the variations in the rheological properties and aggregation behaviour of TEMPO-oxidised cellulose nanofibrils (CNF) aqueous suspensions, as a function of changes in concentration and systematic changes in the pH, by addition of acids with different anions. It was found that CNF suspensions form strong gels at mass fractions higher than 0.35 % and the gel point is ca. 0.18 %. On the other hand, aggregation is enhanced at acidic pH conditions due to lower charge repulsion among fibrils, leading to an increase of the suspension viscosity. However, distinct rheological behaviours were presented by CNF suspensions as different acids were applied. It was found that phosphate ions resulted in significant aggregation leading to formation of particles of large size and very strong gels, at pH 2.3; distinctly, the presence of acetate ions resulted in lower aggregation, lower particle size and weaker gels, at the same pH value.
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Odontomas are benign, non-aggressive, and the most common odontogenic tumor of the jaws. Composed of dental tissues, it can be classified as compound or complex odontomas depending on their radiological and histological features. Among them, complex odontomas are less common and usually is presented as a small and asymptomatic radiopaque mass surrounded by a radiolucent halo, found on routine radiographic examination. Although benign tumors, odontomas can reach large sizes leading to facial asymmetry and decreasing bone strength, which predisposes fractures and infection. Our aim was to present a case report of an unusual giant mandibular odontoma on the left mandibular angle and ramus successfully treated by surgical excision and highlight the importance of the earlier diagnostic to minimize damages.
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Tumores Odontogênicos , Odontoma , Assimetria Facial , Humanos , Mandíbula , Odontoma/diagnóstico por imagem , Odontoma/cirurgia , RadiografiaRESUMO
OBJECTIVES: We aimed to evaluate the effects of cataract surgery on cardiac autonomic modulation. METHODS: A cross-sectional and analytical study was conducted at the Hospital Maria Braido in the city of São Caetano do Sul, São Paulo, between 2015 and 2016. We investigated 19 patients of both sexes who were all over 50 years old; all patients had a diagnosis of senile or bilateral cataracts and were recommended to undergo implantation of the intraocular lens. Heart rate variability (HRV) was evaluated before, during and after cataract surgery. RESULTS: There were no significant changes in the time and geometric domains of HRV before, during or after surgery. The high-frequency (HF) band in normalized units (nu) on the spectral analysis significantly increased (p=0.02, Cohen's d=0.9, large effect size). However, the low-frequency (LF) band in nu significantly decreased during surgery (p=0.02, Cohen's d=0.9, large effect size). CONCLUSION: Throughout the intraocular lens implantation cataract surgery, there was an increase in parasympathetic modulation and a decrease in the sympathetic component of the heart rate (HR). We propose that this result is attributable to the supine position of the patients during surgery and the trigeminal reflex.
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Sistema Nervoso Autônomo/fisiopatologia , Extração de Catarata/efeitos adversos , Frequência Cardíaca/fisiologia , Implante de Lente Intraocular/efeitos adversos , Idoso , Índice de Massa Corporal , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Valores de Referência , Estatísticas não Paramétricas , Fatores de Tempo , Nervo Trigêmeo/fisiopatologiaRESUMO
Biopolymers-based composites are, in general, environmentally friendly materials, which can be obtained from renewable sources. Some of them can also present promising properties to be used in food packaging and electronic devices, being thus logical substitutes to petroleum-based polymers, specifically plastics. Cellulose nanofibrils (CNF) obtained by chemical/enzymatic pre-treatments followed by a mechanical treatment appear as a new suitable biomaterial. However, CNF are still quite expensive materials, due to the required chemicals/equipment/energy involved, and additionally, they present some limitations such as high hydrophilicity/high water vapour permeability. The combination of CNF with clay minerals, such as montmorillonite or kaolinite, as widely available geo-resources, represents an excellent way to reduce the amount of CNF used, enabling the production of valuable materials and reducing costs; and, at the same time it is possible to improve the characteristics of the formed materials, such as mechanical, gas barrier and fire retardancy properties, if appropriate conditions of preparation are used. Nevertheless, to obtain hybrid CNF/clay composites with superior properties it is necessary to ensure a good dispersion of the inorganic material in the CNF suspension and a good compatibility among the inorganic and organic components. To fulfil this goal, several strategies can be considered, including physical treatments of the suspensions, CNF and clay surface chemical modifications, and the use of a coupling agent. In this review article, the state-of-the-art on a new emerging generation of composites (films, foams or coatings) based on nanofibrillated cellulose and nanoclay, with focus on strategies for their preparation and most relevant achievements is critically reviewed, bearing in mind their potential application as substitutes for common plastics. A third component has been eventually added to these organic-inorganic hybrids, e.g., chitosan, carboxymethylcellulose, borate or epoxy resin, to enhance specific characteristics of the material. Some general background on the production of different types of CNF and their main properties is previously provided.
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OBJECTIVES: We aimed to evaluate the effects of cataract surgery on cardiac autonomic modulation. METHODS: A cross-sectional and analytical study was conducted at the Hospital Maria Braido in the city of São Caetano do Sul, São Paulo, between 2015 and 2016. We investigated 19 patients of both sexes who were all over 50 years old; all patients had a diagnosis of senile or bilateral cataracts and were recommended to undergo implantation of the intraocular lens. Heart rate variability (HRV) was evaluated before, during and after cataract surgery. RESULTS: There were no significant changes in the time and geometric domains of HRV before, during or after surgery. The high-frequency (HF) band in normalized units (nu) on the spectral analysis significantly increased (p=0.02, Cohen's d=0.9, large effect size). However, the low-frequency (LF) band in nu significantly decreased during surgery (p=0.02, Cohen's d=0.9, large effect size). CONCLUSION: Throughout the intraocular lens implantation cataract surgery, there was an increase in parasympathetic modulation and a decrease in the sympathetic component of the heart rate (HR). We propose that this result is attributable to the supine position of the patients during surgery and the trigeminal reflex.
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Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Sistema Nervoso Autônomo/fisiopatologia , Extração de Catarata/efeitos adversos , Implante de Lente Intraocular/efeitos adversos , Frequência Cardíaca/fisiologia , Valores de Referência , Fatores de Tempo , Nervo Trigêmeo/fisiopatologia , Índice de Massa Corporal , Estudos Transversais , Estudos Prospectivos , Estatísticas não ParamétricasRESUMO
The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects underwent spirometry, bronchial challenge test with mannitol, skin prick tests, and questionnaires about animal exposures and respiratory symptoms. Control group comprised 125 subjects; cat owner group, 51 subjects; and dog owner group, 140 subjects. Cat owners had increased asthma prevalence (defined by symptoms and positive bronchial challenge test), but no changes in lung function compared to the control group. The dog owner group had lower spirometry values (forced expiratory volume in one second and lower forced vital capacity), but similar asthma prevalence, compared to the control group. In the cat owner group, excess of asthma may have an immunological basis, since we found an association with atopy. Although we did not have endotoxin data from volunteers' households, we postulated that low values of lung function were associated to exposure to endotoxins present in environments exposed to dogs.
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Asma/epidemiologia , Asma/etiologia , Gatos , Cães , Pulmão/fisiopatologia , Propriedade/estatística & dados numéricos , Animais de Estimação , Adolescente , Adulto , Alérgenos/efeitos adversos , Análise de Variância , Animais , Asma/fisiopatologia , Brasil/epidemiologia , Criança , Estudos Transversais , Exposição Ambiental/efeitos adversos , Feminino , Volume Expiratório Forçado/fisiologia , Humanos , Masculino , Prevalência , Valores de Referência , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/etiologia , Medição de Risco , Fatores de Risco , Testes Cutâneos , Espirometria , Capacidade Vital/fisiologia , Adulto JovemRESUMO
Amazon mosses, such as Holomitriopsis laevifolia and Leucobryum sp. are naturally exposed to high levels of solar ultraviolet (UV) radiation. Theoretically, under environmental stress conditions these mosses have developed protective chemical and metabolic strategies against UV damage, by way of biosynthesis of secondary metabolites, such as flavonoids. The present paper aimed to evaluate the free-radical scavenging activity, and the photoprotective, mutagenic and photomutagenic potencies of the methanolic (ME), aqueous (AE), hydroalcoholic (HE), ethanolic (EE) extracts of H. laevifolia and Leucobryum sp. The phenolic contents were evaluated by spectrophotometry and by High-Performance Liquid Chromatography (HPLC). The present findings showed that the AE and HE of H. laevifolia and the AE of Leucobryum sp. presented the highest phenolic contents. The HPLC analysis indicated the presence mainly of phenolic and cinnamic acids, flavonols, flavones and flavanones. The AE and EE of H. laevifolia and the AE and HE of Leucobryum sp. efficiently scavenged the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical. All extracts showed significant values of in vitro Sun Protection Factor alone, and HE of Leucobryum sp. showed a synergistic effect in association with benzophenone-3. None of the extracts induced mutagenicity in the auxotrophic strains for histidine of Salmonella typhimurium, and photomutagenicity of the TA102 and TA104 strains was not detected after exposure to UV-A radiation. Besides, all extracts showed photoprotective activity against UV-A radiation for the TA104 strain, including synergistic protection in association with BP-3. Thus, the constituents in H. Laevifolia and Leucobryum sp. could be good candidates for cosmetic and dermatological applications, particularly in association with synthetic UV filters, since the concentration of the filters in the final product could be reduced.
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Briófitas/química , Dano ao DNA/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Raios Ultravioleta , Briófitas/metabolismo , Cromatografia Líquida de Alta Pressão , Dano ao DNA/efeitos da radiação , Flavonoides/análise , Flavonoides/química , Sequestradores de Radicais Livres/química , Testes de Mutagenicidade , Estresse Oxidativo/efeitos da radiação , Fenóis/análise , Fenóis/química , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genética , Espectrofotometria , Fator de Proteção SolarRESUMO
The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects underwent spirometry, bronchial challenge test with mannitol, skin prick tests, and questionnaires about animal exposures and respiratory symptoms. Control group comprised 125 subjects; cat owner group, 51 subjects; and dog owner group, 140 subjects. Cat owners had increased asthma prevalence (defined by symptoms and positive bronchial challenge test), but no changes in lung function compared to the control group. The dog owner group had lower spirometry values (forced expiratory volume in one second and lower forced vital capacity), but similar asthma prevalence, compared to the control group. In the cat owner group, excess of asthma may have an immunological basis, since we found an association with atopy. Although we did not have endotoxin data from volunteers' households, we postulated that low values of lung function were associated to exposure to endotoxins present in environments exposed to dogs.
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Humanos , Animais , Masculino , Feminino , Criança , Adolescente , Adulto , Gatos , Cães , Adulto Jovem , Propriedade/estatística & dados numéricos , Asma/etiologia , Asma/epidemiologia , Animais de Estimação , Pulmão/fisiopatologia , Valores de Referência , Hipersensibilidade Respiratória/epidemiologia , Asma/fisiopatologia , Espirometria , Brasil/epidemiologia , Alérgenos/efeitos adversos , Volume Expiratório Forçado/fisiologia , Prevalência , Estudos Transversais , Fatores de Risco , Análise de Variância , Exposição Ambiental/efeitos adversosRESUMO
BACKGROUND: Workers exposed to laboratory animals have a high risk of developing laboratory animal allergy (LAA). Atopy seems to be the main risk factor for LAA. We hypothesized that occupational sensitization is a better predictor for the development of asthma, rhinitis, and bronchial hyperresponsiveness (BHR) than common sensitization. OBJECTIVE: To investigate the association between occupational sensitization to laboratory animals and clinical outcomes. METHODS: This was a cross-sectional study performed at two universities on students and employees dealing with small rodents. The subjects were allocated in groups: non-sensitized, common sensitization, or occupational sensitization, according to the results of the skin prick test (SPT). All subjects answered a questionnaire about animal exposures, symptoms, allergic diseases, and underwent spirometry and bronchial challenge test with mannitol. Multivariate analysis was performed using Poisson regression to estimate the prevalence ratio (PR). RESULTS: Data from 453 volunteers were analysed. Non-sensitized group comprised 237 subjects; common sensitization group, 142 subjects; and occupational sensitization group, 74 subjects. Occupational sensitization was associated with greater risk for all outcomes studied. When the common sensitization group was reference, skin symptoms had PR of 1.36, 95% confidence interval (CI): 1.01-1.85; wheezing had PR of 1.75, CI 95%: 1.21-2.53; rhinitis had PR of 1.25, 95%: 1.11-1.40; nocturnal dyspnoea had PR of 2.40, 95% CI: 1.31-4.40; bronchial hyperresponsiveness (BHR) had PR of 2.47, 95% CI: 1.50-4.09; and confirmed asthma had PR of 2.65, 95% CI: 1.45-4.85. In addition, the overlap of asthma, rhinitis, and skin symptoms in a same subject was significantly more prevalent in the occupational sensitization group, 16.2% versus 4.9% in the common sensitization group. CONCLUSION AND CLINICAL RELEVANCE: Occupational sensitization is associated with allergic symptoms and respiratory diseases. SPT with occupational allergens along with other parameters may contribute to detection of risk for allergic and respiratory diseases associated with exposure to laboratory animals.
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Alérgenos/imunologia , Animais de Laboratório , Asma/imunologia , Exposição Ocupacional , Rinite Alérgica/imunologia , Pele/imunologia , Pele/patologia , Adulto , Animais , Animais de Laboratório/imunologia , Asma/diagnóstico , Asma/epidemiologia , Feminino , Humanos , Imunização , Masculino , Exposição Ocupacional/efeitos adversos , Prevalência , Rinite Alérgica/diagnóstico , Rinite Alérgica/epidemiologia , Fatores de Risco , Testes Cutâneos , Inquéritos e Questionários , Adulto JovemRESUMO
Coffee is one of the most widely consumed beverages throughout the world. So far, many studies have shown the properties of coffee beverages, but little is known about its impacts on human and environmental health from its discard in the environment. So, the present work aims to investigate the mutagenic, genotoxic, cytotoxic and ecotoxic effects of leached (LE) and solubilized (SE) extracts from coffee waste, simulating the disposal of this residue in landfills and via sewage systems, respectively. Chemical analyses were also carried out. LE and SE induced mutagenicity in the TA98 Salmonella strain with and without exogenous metabolization (S9). In the TA100 only SE induced mutagenicity, what was observed without S9. An increase in the frequency of micronuclei was observed in HepG2 cell line after 3 and 24h of exposure to both extracts. No cytotoxic effects were observed in HepG2 cells by WST-1 assay. The EC50 values for the LE and SE were 1.5% and 11.26% for Daphnia similis, 0.12% and 1.39% for Ceriodaphnia dubia and 6.0% and 5.5% for Vibrio fischeri, respectively. Caffeine and several transition metals were found in both extracts. Coffee waste discarded in the environment may pose a risk to human and environmental health, since this compound can cause DNA damage and present toxicity to aquatic organisms.
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Organismos Aquáticos/efeitos dos fármacos , Café/química , Mutagênicos/toxicidade , Resíduos/análise , Poluentes Químicos da Água/toxicidade , Aliivibrio fischeri/efeitos dos fármacos , Animais , Organismos Aquáticos/genética , Bioensaio , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA , Daphnia/efeitos dos fármacos , Saúde Ambiental , Células Hep G2 , Humanos , Testes de Mutagenicidade , Salmonella/efeitos dos fármacos , Esgotos/química , Testes de Toxicidade/métodosRESUMO
LLL-3, an anthracene derived compound, has been shown to be a promising therapeutic agent for the treatment of some kinds of cancer such as chronic myeloid leukemia and glioblastoma. However, no data regarding the toxic properties of this compound have yet been described in the literature. The present work aimed to investigate the mutagenic and genotoxic activities of LLL-3 using the TA97, TA98, TA100, TA102 and TA104 Salmonella/microsome strains for the Ames test and the micronucleus assay with the mouse macrophage cell line RAW 264.7. The findings showed that LLL-3, at doses of 0.001, 0.01, 0.1, 1.0 and 10.0 µg/plate, did not induce mutagenic activity in the Salmonella strains used under the conditions tested, and nor did it present genotoxicity in RAW 264.7 cells, at 10.0, 100.0 and 1000.0 µg/mL doses. Moreover, it is important to point out that the mitotic index of the cells decreased after exposure to LLL-3 under the same conditions tested, which may suggest some cytostatic effect, since this compound acts by inhibiting STAT3. Since most drugs used in the treatment of cancer present mutagenic activity as an adverse effect, these results suggest that LLL-3 is a promising drug for cancer therapy.
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Antraquinonas/toxicidade , Antineoplásicos/toxicidade , Micronúcleos com Defeito Cromossômico/induzido quimicamente , Fator de Transcrição STAT3/antagonistas & inibidores , Salmonella typhimurium/efeitos dos fármacos , Animais , Antraquinonas/farmacologia , Antineoplásicos/farmacologia , Técnicas de Cultura de Células , Linhagem Celular , Relação Dose-Resposta a Droga , Macrófagos/efeitos dos fármacos , Macrófagos/patologia , Camundongos , Testes para Micronúcleos , Testes de Mutagenicidade , Salmonella typhimurium/genéticaRESUMO
Antarctica moss Sanionia uncinata (Hedw.) Loeske is exposed in situ to damaging levels of ultraviolet (UV) radiation. This moss has the ability to respond to UV radiation exposure producing secondary metabolites such as flavonoids, and has been recommended as a potential source of photoprotective compounds and antioxidants. The aim of the present paper was to investigate the free-radical scavenging activity and mutagenic and photomutagenic properties of methanolic (ME), hydroethanolic (HE) and ethanolic (EE) extracts of S. uncinata. The phenolic contents were evaluated by high-performance liquid chromatography (HPLC) and spectrophotometry. The findings showed that ME and EE presented the highest phenolic contents and inhibited free radical-scavenging activity against 2,2'-diphenyl-1 picrylhydrazyl (DPPH) and the HPLC analysis indicated several classes of phenolic acids and flavonoids. The sun protection factors (SPF) were determined by an in vitro method and the results showed significant values. The SPF values of BZ-3 at 50µg/mL increased significantly in association with ME, HE and EE. The extracts did not induce mutagenicity in auxotrophic Salmonella typhimurium histidine and photomutagenicity was not detected in the TA102 and TA104 strains after exposure to UV-A at doses of up to 6.5J/cm2 for the TA102 strain and up to 0.24J/cm2 for the TA104 strain. In addition, with the exception of ME, all the extracts induced photoprotective effects in the presence of the TA104 strain at 0.04J/cm2. The present results suggest that S. uncinata extracts did not induce photomutation and showed promise for photoprotection against the photobiological and ROS-inducing effects of the UV-A radiation.
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Briófitas , Extratos Vegetais/efeitos da radiação , Protetores Solares/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Animais , Regiões Antárticas , Relação Dose-Resposta a Droga , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/efeitos da radiação , Sequestradores de Radicais Livres/toxicidade , Testes de Mutagenicidade/métodos , Mutagênicos/toxicidade , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Ratos , Salmonella typhimurium , Protetores Solares/isolamento & purificação , Protetores Solares/toxicidadeRESUMO
Variation in annual rainfall is considered the most important factor influencing population dynamics in dry environments. However, different factors may control population dynamics in different microhabitats. This study recognizes that microhabitat variation may attenuate the influence of climatic seasonality on the population dynamics of herbaceous species in dry forest (Caatinga) areas of Brazil. We evaluated the influence of three microhabitats (flat, rocky and riparian) on the population dynamics of four herbaceous species (Delilia biflora, Commelina obliqua, Phaseolus peduncularis and Euphorbia heterophylla) in a Caatinga (dry forest) fragment at the Experimental Station of the Agronomic Research Institute of Pernambuco in Brazil, over a period of three years. D. biflora, C. obliqua and P. peduncularis were found in all microhabitats, but they were present at low densities in the riparian microhabitat. There was no record of E. heterophylla in the riparian microhabitat. Population size, mortality rates and natality rates varied over time in each microhabitat. This study indicates that different establishment conditions influenced the population size and occurrence of the four species, and it confirms that microhabitat can attenuate the effect of drought stress on mortality during the dry season, but the strength of this attenuator role may vary with time and species.
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Clima Desértico , Florestas , Magnoliopsida/fisiologia , Brasil , Densidade Demográfica , Estações do Ano , Especificidade da EspécieRESUMO
Abstract Variation in annual rainfall is considered the most important factor influencing population dynamics in dry environments. However, different factors may control population dynamics in different microhabitats. This study recognizes that microhabitat variation may attenuate the influence of climatic seasonality on the population dynamics of herbaceous species in dry forest (Caatinga) areas of Brazil. We evaluated the influence of three microhabitats (flat, rocky and riparian) on the population dynamics of four herbaceous species (Delilia biflora, Commelina obliqua, Phaseolus peduncularis and Euphorbia heterophylla) in a Caatinga (dry forest) fragment at the Experimental Station of the Agronomic Research Institute of Pernambuco in Brazil, over a period of three years. D. biflora, C. obliqua and P. peduncularis were found in all microhabitats, but they were present at low densities in the riparian microhabitat. There was no record of E. heterophylla in the riparian microhabitat. Population size, mortality rates and natality rates varied over time in each microhabitat. This study indicates that different establishment conditions influenced the population size and occurrence of the four species, and it confirms that microhabitat can attenuate the effect of drought stress on mortality during the dry season, but the strength of this attenuator role may vary with time and species.
Resumo A variação anual na precipitação é considerada o fator mais importante que influencia a dinâmica populacional em ambientes secos. No entanto, diferentes fatores podem controlar a dinâmica populacional em diferentes microhabitats. A hipótese deste estudo é que as variações de microhabitats podem atenuar a influência da sazonalidade climática sobre a dinâmica populacional de espécies herbáceas em uma áreas floresta seca (Caatinga) no Brasil. Então, objetivou-se avaliar a influência de três microhabitats (plano, rochoso e ciliar) sobre a dinâmica das populações de quatro espécies herbáceas (Delilia biflora, Commelina obliqua, Phaseolus peduncularis e Euphorbia heterophylla) em um fragmento de Caatinga da Estação Experimental do Instituto de Pesquisas Agronômicas de Pernambuco, no Brasil, durante um período de três anos. D. biflora, C. obliqua e P. peduncularis foram encontrados em todos os microhabitats, mas elas estavam presentes em baixas densidades no microhabitat ciliar. Não houve registro de E. heterophylla no microhabitat ciliar. O tamanho das populações, as taxas de mortalidade e de natalidade variaram ao longo do tempo em cada microhabitat. Este estudo indica que diferentes as condições de estabelecimento influenciaram o tamanho das populações e a ocorrência das quatro espécies e confirma que microhabitat pode atenuar o efeito do estresse hídrico sobre a mortalidade durante a estação seca, mas a força desse papel atenuador pode variar com o tempo e a espécie considerada.
Assuntos
Magnoliopsida/fisiologia , Clima Desértico , Florestas , Brasil , Densidade Demográfica , Estações do Ano , Especificidade da EspécieRESUMO
OBJECTIVE: Fresh-frozen bone allograft (FFBA) is an alternative to autogenous bone (AB) for reconstructing maxillary bone. Despite the promising clinical results, cell responses to FFBA and AB were not evaluated. Thus, our aim was to compare cells harvested from maxillary reconstructed sites with either AB or FFBA in terms of osteoblast differentiation and to evaluate the effect of culturing cells in contact with FFBA. METHODS: Cells harvested from three patients submitted to bilateral maxillary reconstruction with AB and FFBA were cultured to evaluate: proliferation, alkaline phosphatase activity, extracellular matrix mineralization and gene expression of osteoblastic markers. The effect of FFBA on osteoblast differentiation was studied by culturing cells harvested from AB in contact with FFBA and evaluating the same parameters. Data were compared using either two-way ANOVA followed by Tukey-b test or Student's t test (p≤0.05). RESULTS: Cell proliferation was higher in cultures from AB grafted sites and extracellular matrix mineralization was higher in cultures derived from FFBA grafted sites. The gene expression of alkaline phosphatase, RUNX2, bone sialoprotein and osteocalcin was higher in cells derived from FFBA compared with cells from AB grafted sites. However, the exposure of cells derived from AB to FFBA particles did not have any remarkable effect on osteoblast differentiation. CONCLUSIONS: These results indicate the higher osteogenic activity of cells derived from FFBA compared with AB reconstructed sites, offering an explanation at cellular level of why FFBA could be a suitable alternative to AB for reconstructing maxillary bone defects.
Assuntos
Transplante Ósseo/métodos , Criopreservação , Reconstrução Mandibular/métodos , Fosfatase Alcalina/biossíntese , Aloenxertos/transplante , Regeneração Óssea/genética , Transplante Ósseo/efeitos adversos , Diferenciação Celular/fisiologia , Proliferação de Células/fisiologia , Células Cultivadas , Feminino , Humanos , Pessoa de Meia-Idade , Osteoblastos/citologia , Osteoblastos/fisiologia , Osteocalcina/biossínteseRESUMO
Proximity to forests contributes to the recolonisation of anthropogenic-disturbed areas through seed input. We evaluated the role of proximity to a mature forest in the recolonisation of an agricultural area that has been abandoned for 18 years and is currently a young forest. Seed rain was monitored at fixed distances from the mature forest. The type of surface recolonisation (germination versus resprouting) and the reproductive season were measured in both forests. The majority of plants recolonising the young forest originated from seed germination. Proximity to the mature forest contributed to the seed rain in the young forest; however, 18 years has not provided sufficient time for the recolonisation of 80 species present in the mature forest. Some species shared between forests differed in their fruiting season and seed dispersal. The seed rain had a total species richness of 56, a total density of 2270 seeds·m(-2)·year(−1) and predominance of self- and wind dispersal. A significant reduction in seed rain with increasing distance from the mature forest was observed. The young forest contained 35 species not observed in the mature forest, and the floristic similarity between the two forests was 0.5, indicating that the two forests are floristically distinct.
Assuntos
Agricultura , Clima , Sementes/fisiologia , FlorestasRESUMO
This study systematically reviewed the systematic production about domestic violence, based on the professional practice of the dentist in a multidisciplinary and interdisciplinary context, from the perspective of public health. The goal was to verify how this issue has been addressed, identify which is the most explored focus, and point out possible gaps. The search was conducted in the Virtual Health Library (BVS Brazil), by selecting 40 articles: 37 on Lilacs, 2 in BBO and only 1 in SciELO, from 2000 to 2011. Of the total scientific production analyzed, 20 percent references have cited the dentist along the paper, among these 50 percent pointed for the practice of the dentist in a multidisciplinary and interdisciplinary context, from the perspective of public health. The health professional role in a multidisciplinary and interdisciplinary context, from the perspective of public health corresponded to 72.5 percent studies. In this way, the need for studies on the performance of professional dentist in interdisciplinary and multidisciplinary team is necessary before the Brazilian context, being an open and wide field to be explored, from the perspective of public health.
Este estudio revisa sistemáticamente lo publicado sobre violencia doméstica, basado en la práctica profesional del dentista en un contexto multidisciplinario e interdisciplinario, desde la perspectiva de salud pública. El objetivo consiste en verificar cómo ha sido abordado este tema, identificar cuál es el foco más explorado y señalar posibles vacíos. Se realizó la investigación usando la Biblioteca Virtual de Salud (BVS Brasil), seleccionando 40 artículos: 37 en Lilacs, 2 en BBO y solamente 1 en SciELO, del 2000 al 2011. Del total de la producción científica analizada, 20 por ciento de la referencias han citado al dentista en el artículo; entre estos, 50 por ciento señalan la práctica del dentista en un contexto multidisciplinario e interdisciplinario, desde la perspectiva de salud pública. El papel del profesional de la salud en un contexto multidisciplinario e interdisciplinario desde la perspectiva de salud púbica correspondía al 72,5 por ciento de los estudios. De esta forma, se necesitan estudios sobre el comportamiento de dentistas profesionales en equipos multidisciplinarios e interdisciplinarios en el contexto de Brasil, siendo un campo abierto y amplio para ser explorado, desde la perspectiva de salud pública.
Este estudo é uma revisão da produção sistemática sobre violência doméstica, baseado na prática profissional do dentista num contexto multidisciplinar e interdisciplinar, sob a perspectiva da saúde pública. O objetivo foi verificar como este tema tem sido abordado, identificar qual é o foco mais explorado, e apontar as possíveis lacunas. A pesquisa foi realizada na Biblioteca Virtual de Saúde (BVS Brazil), por seleção de 40 artigos: 37 do Lilacs, 2 do BBO e somente 1 do SciELO, no período de 2000 a 2011. Do total da produção científica analisada, 20 por cento citaram referências de dentistas no texto, destes 50 por cento apontaram para a prática do dentista num contexto multi e interdisciplinar, sob a perspectiva da saúde pública. O papel do profissional de saúde no contexto multi e interdisciplinar, sob a perspectiva da saúde pública, correspondeu a 72.5 por cento estudos. Daí a necessidade para estudos da atividade do profissional dentista na inter e multidisciplinar é importante no contexto brasileiro, abrindo um amplo campo a ser explorado sob o ponto de vista da saúde pública.