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1.
Elife ; 62017 10 16.
Artigo em Inglês | MEDLINE | ID: mdl-29035697

RESUMO

The underground environment imposes unique demands on life that have led subterranean species to evolve specialized traits, many of which evolved convergently. We studied convergence in evolutionary rate in subterranean mammals in order to associate phenotypic evolution with specific genetic regions. We identified a strong excess of vision- and skin-related genes that changed at accelerated rates in the subterranean environment due to relaxed constraint and adaptive evolution. We also demonstrate that ocular-specific transcriptional enhancers were convergently accelerated, whereas enhancers active outside the eye were not. Furthermore, several uncharacterized genes and regulatory sequences demonstrated convergence and thus constitute novel candidate sequences for congenital ocular disorders. The strong evidence of convergence in these species indicates that evolution in this environment is recurrent and predictable and can be used to gain insights into phenotype-genotype relationships.


Assuntos
Adaptação Biológica , Evolução Biológica , Ecossistema , Olho , Mamíferos , Fenômenos Fisiológicos Oculares , Animais
2.
Mol Biol Evol ; 33(1): 93-108, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26420451

RESUMO

The human kallikrein (KLK) cluster, located at chromosome 19q13.3-13.4, encodes 15 serine proteases, including neighboring genes (KLK3, KLK2, KLK4, and KLK5) with key roles in the cascades of semen liquefaction, tooth enamel maturation, and skin desquamation. KLK2 and KLK3 were previously identified as targets of adaptive evolution in primates through different mechanisms linked to reproductive biology and, in humans, genome-wide scans of positive selection captured, a yet unexplored, evidence for KLK neutrality departure in East Asians. We perform a detailed evaluation of KLK3-KLK5 variability in the 1000 Genomes samples from East Asia, Europe, and Africa, which was sustained by our own sequencing. In East Asians, we singled out a 70-kb region surrounding KLK4 that combined unusual low levels of diversity, high frequency variants with significant levels of population differentiation (FST > 0.5) and fairly homogenous haplotypes given the large local recombination rates. Among these variants, rs1654556_G, rs198968_T, and rs17800874_A stand out for their location on putative regulatory regions and predicted functional effects, namely the introduction of several microRNA binding sites and a repressor motif. Our functional assays carried out in different cellular models showed that rs198968_T and rs17800874_A operate synergistically to reduce KLK4 expression and could be further assisted by rs1654556_G. Considering the previous findings that KLK4 inactivation causes enamel malformations in humans and mice, and that this gene is coexpressed in epidermal layers along with several substrates involved in either cell adhesion or keratinocyte differentiation, we propose KLK4 as another target of selection in East Asians correlated to tooth and epidermal morphological traits.


Assuntos
Povo Asiático/genética , Regulação para Baixo/genética , Variação Genética/genética , Calicreínas/genética , Evolução Molecular , Ásia Oriental , Humanos , Seleção Genética/genética
3.
Genome Biol Evol ; 5(12): 2512-23, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24356879

RESUMO

Recent efforts have attempted to describe the population structure of common chimpanzee, focusing on four subspecies: Pan troglodytes verus, P. t. ellioti, P. t. troglodytes, and P. t. schweinfurthii. However, few studies have pursued the effects of natural selection in shaping their response to pathogens and reproduction. Whey acidic protein (WAP) four-disulfide core domain (WFDC) genes and neighboring semenogelin (SEMG) genes encode proteins with combined roles in immunity and fertility. They display a strikingly high rate of amino acid replacement (dN/dS), indicative of adaptive pressures during primate evolution. In human populations, three signals of selection at the WFDC locus were described, possibly influencing the proteolytic profile and antimicrobial activities of the male reproductive tract. To evaluate the patterns of genomic variation and selection at the WFDC locus in chimpanzees, we sequenced 17 WFDC genes and 47 autosomal pseudogenes in 68 chimpanzees (15 P. t. troglodytes, 22 P. t. verus, and 31 P. t. ellioti). We found a clear differentiation of P. t. verus and estimated the divergence of P. t. troglodytes and P. t. ellioti subspecies in 0.173 Myr; further, at the WFDC locus we identified a signature of strong selective constraints common to the three subspecies in WFDC6-a recent paralog of the epididymal protease inhibitor EPPIN. Overall, chimpanzees and humans do not display similar footprints of selection across the WFDC locus, possibly due to different selective pressures between the two species related to immune response and reproductive biology.


Assuntos
Imunidade Inata/genética , Pan troglodytes/genética , Reprodução/genética , Proteínas Secretadas pela Vesícula Seminal/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Evolução Molecular , Variação Genética , Humanos , Masculino , Polimorfismo de Nucleotídeo Único , Seleção Genética , Alinhamento de Sequência , Análise de Sequência de DNA
4.
PLoS One ; 8(6): e66889, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23826168

RESUMO

Serine protease inhibitors (SERPINs) are a superfamily of highly conserved proteins that play a key role in controlling the activity of proteases in diverse biological processes. The SERPIN cluster located at the 14q32.1 region includes the gene coding for SERPINA1, and a highly homologous sequence, SERPINA2, which was originally thought to be a pseudogene. We have previously shown that SERPINA2 is expressed in different tissues, namely leukocytes and testes, suggesting that it is a functional SERPIN. To investigate the function of SERPINA2, we used HeLa cells stably transduced with the different variants of SERPINA2 and SERPINA1 (M1, S and Z) and leukocytes as the in vivo model. We identified SERPINA2 as a 52 kDa intracellular glycoprotein, which is localized at the endoplasmic reticulum (ER), independently of the variant analyzed. SERPINA2 is not significantly regulated by proteasome, proposing that ER localization is not due to misfolding. Specific features of SERPINA2 include the absence of insoluble aggregates and the insignificant response to cell stress, suggesting that it is a non-polymerogenic protein with divergent activity of SERPINA1. Using phylogenetic analysis, we propose an origin of SERPINA2 in the crown of primates, and we unveiled the overall conservation of SERPINA2 and A1. Nonetheless, few SERPINA2 residues seem to have evolved faster, contributing to the emergence of a new advantageous function, possibly as a chymotrypsin-like SERPIN. Herein, we present evidences that SERPINA2 is an active gene, coding for an ER-resident protein, which may act as substrate or adjuvant of ER-chaperones.


Assuntos
Variação Genética , alfa 1-Antitripsina/genética , Retículo Endoplasmático/efeitos dos fármacos , Retículo Endoplasmático/metabolismo , Perfilação da Expressão Gênica , Variação Genética/efeitos dos fármacos , Células HeLa , Resposta ao Choque Térmico/efeitos dos fármacos , Humanos , Funções Verossimilhança , Modelos Moleculares , Chaperonas Moleculares/metabolismo , Filogenia , Polimerização/efeitos dos fármacos , Inibidores de Proteassoma/farmacologia , Ligação Proteica/efeitos dos fármacos , Dobramento de Proteína/efeitos dos fármacos , Transporte Proteico/efeitos dos fármacos , Proteólise/efeitos dos fármacos , Proteínas Recombinantes/metabolismo , Frações Subcelulares/efeitos dos fármacos , Frações Subcelulares/metabolismo , alfa 1-Antitripsina/metabolismo
5.
Mol Biol Evol ; 30(4): 938-50, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23292442

RESUMO

The whey acidic protein (WAP) four-disulfide core domain (WFDC) locus located on human chromosome 20q13 spans 19 genes with WAP and/or Kunitz domains. These genes participate in antimicrobial, immune, and tissue homoeostasis activities. Neighboring SEMG genes encode seminal proteins Semenogelin 1 and 2 (SEMG1 and SEMG2). WFDC and SEMG genes have a strikingly high rate of amino acid replacement (dN/dS), indicative of responses to adaptive pressures during vertebrate evolution. To better understand the selection pressures acting on WFDC genes in human populations, we resequenced 18 genes and 54 noncoding segments in 71 European (CEU), African (YRI), and Asian (CHB + JPT) individuals. Overall, we identified 484 single-nucleotide polymorphisms (SNPs), including 65 coding variants (of which 49 are nonsynonymous differences). Using classic neutrality tests, we confirmed the signature of short-term balancing selection on WFDC8 in Europeans and a signature of positive selection spanning genes PI3, SEMG1, SEMG2, and SLPI. Associated with the latter signal, we identified an unusually homogeneous-derived 100-kb haplotype with a frequency of 88% in Asian populations. A putative candidate variant targeted by selection is Thr56Ser in SEMG1, which may alter the proteolytic profile of SEMG1 and antimicrobial activities of semen. All the well-characterized genes residing in the WDFC locus encode proteins that appear to have a role in immunity and/or fertility, two processes that are often associated with adaptive evolution. This study provides further evidence that the WFDC and SEMG loci have been under strong adaptive pressure within the short timescale of modern humans.


Assuntos
Cromossomos Humanos Par 20/genética , Fertilidade/genética , Imunidade/genética , Polimorfismo de Nucleotídeo Único , Seleção Genética , Adaptação Biológica/genética , Substituição de Aminoácidos , Evolução Molecular , Frequência do Gene , Haplótipos , Humanos , Desequilíbrio de Ligação , Modelos Genéticos , Análise de Componente Principal , Proteínas/genética , Proteínas Secretadas pela Vesícula Seminal/genética , Análise de Sequência de DNA
6.
PLoS One ; 7(2): e32518, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22393410

RESUMO

Serine protease inhibitors (SERPINs) are crucial in the regulation of diverse biological processes including inflammation and immune response. SERPINB11, located in the 18q21 gene cluster, is a polymorphic gene/pseudogene coding for a non-inhibitory SERPIN. In a genome-wide scan for recent selection, SERPINB11 was identified as a potential candidate gene for adaptive evolution in Yoruba. The present study sought a better understanding of the evolutionary history of SERPINB11, with special focus on evaluating its selective signature. Through the resequencing of coding and noncoding regions of SERPINB11 in 20 Yorubans and analyzing primate orthologous sequences, we identified a full-length SERPINB11 variant encoding a non-inhibitory SERPIN as the putative candidate of selection--probably driven to higher frequencies by an adaptive response using preexisting variation. In addition, we detected contrasting evolutionary features of SERPINB11 in primates: While primate phylogeny as a whole is under purifying selection, the human lineage shows evidence of positive selection in a few codons, all associated with the active SERPINB11. Comparative modeling studies suggest that positively selected codons reduce SERPINB11's ability to undergo the conformational changes typical of inhibitory SERPINs--suggesting that it is evolving towards a new non-inhibitory function in humans. Significant correlations between SERPINB11 variants and the environmental variables, pastoralism and pathogen richness, have led us to propose a selective advantage through host-pathogen interactions, possibly linked to an adaptive response combating the emergence of infectious diseases in recent human evolution. This work represents the first description of a resurrected gene in humans, and may well exemplify selection on standing variation triggered by drastic ecological shifts.


Assuntos
Interações Hospedeiro-Patógeno , Serpinas/genética , Alelos , Animais , Mapeamento Cromossômico/métodos , Códon , Evolução Molecular , Variação Genética , Haplótipos , Humanos , Modelos Genéticos , Família Multigênica , Filogenia , Polimorfismo Genético , Especificidade da Espécie
7.
Mol Biol Evol ; 28(10): 2811-22, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21536719

RESUMO

The whey acidic protein four-disulfide core (WFDC) gene cluster on human chromosome 20q13, harbors 15 small serine protease inhibitor genes with roles in innate immunity, reproduction, and regulation of endogenous proteases kallikreins. The WFDC cluster has emerged as a prime example of rapid diversification and adaptive evolution in primates. This study sought a better understanding of the evolutionary history of WFDC genes in humans and focused on exploring the adaptive selection signatures found in populations of European (Utah residents with ancestry from northern and western Europe [CEU]) and African (Yoruba from Ibadan, in Nigeria [YRI]) ancestry in a genome-wide scan for putative targets of recent adaptive selection. Our approach included resequencing coding and noncoding regions of WFDC6, EPPIN, and WFDC8 in 20 CEU and of SPINT4 in 20 YRI individuals. We generated 302 kb and 60 kb of high-quality sequence data from CEU and of YRI populations, respectively, enabling the identification of 72 single nucleotide polymorphisms. Using classic neutrality tests, empirical and haplotype-based analysis, we pinpointed WFDC8 and SPINT4 as the likely targets of short-term balancing selection in the CEU population, and recent positive selection (incomplete selective sweep) in the YRI population. Putative candidate variants targeted by selection include 44A (rs7273669A) for WFDC8, which may downregulate gene expression by abolishing the binding site of two transcription factors; and a haplotype configuration [Ser73+98A] (rs6017667A-rs6032474A) for SPINT4, which may simultaneously affect protein function and gene regulation. We propose that the evolution of WFDC8 and SPINT4 has been shaped by complex selective scenarios due to the interdependence of variant fitness and ecological variables.


Assuntos
População Negra/genética , Evolução Molecular , Proteínas do Leite/genética , Família Multigênica , Inibidores de Serina Proteinase/genética , População Branca/genética , Adaptação Biológica , Humanos , Imunidade Inata , Polimorfismo de Nucleotídeo Único , Seleção Genética
8.
Arq. Centro Estud. Curso Odontol ; 27(1/2): 63-73, jan.-dez. 1990. tab, ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-119996

RESUMO

A proposiçäo deste trabalho é verificar a influência da pressäo positiva no tempo de presa inicial e final, assim como a variaçäo de volume em decorrência da pressäo exercida, em um revestimento aglutinado por gesso encontrado no comércio odontológico. Foram obtidos setenta e seis corpos de prova, sendo cinco pela técnica de inclusäo convencional e setenta e um pela técnica pressäo. Para a verificaçäo do tempo de presa foram utilizadas agulhas GILMORE, enquanto que, para a variaçäo volumétrica aplicou-se uma fórmula matemática. Diante dos resultados obtidos concluiu-se que a utilizaçäo da pressäo positiva influenciou o tempo de presa inicial e final, bem como o seu volume após a presa


Assuntos
Sulfato de Cálcio , Revestimento para Fundição Odontológica
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