Scotopic Contour Deformation Detection Reveals Early Rod Dysfunction in Age-Related Macular Degeneration With and Without Reticular Pseudodrusen.

Purpose: The purpose of this study was to investigate scotopic contour deformation detection (sCDD), and its structural determinants, in participants with intermediate age-related macular degeneration (iAMD) with or without reticular pseudodrusen (RPD). Methods: Forty-one participants (aged 58-89 years), including 9 with iAMD and RPD, 16 with iAMD only, and 16 controls, underwent functional testing. The sCDD was evaluated with radial frequency arcs presented at 4 loci: ±4 degrees and 8 degrees vertical eccentricity. Scotopic thresholds and dark adaptation (DA) were measured at the same loci. Retinal layers of spectral domain optical coherence tomography (SD-OCT) volume scans were segmented. To establish the concurrent validity of the functional test, we evaluated the fraction of variability in sCDD thresholds explained by SD-OCT data. Results: The iAMD group had significantly worse sCDD thresholds compared with controls (8 degrees inferior retina: P = 0.004 and the 4 degrees loci: P < 0.02 for both). Elevated sCDD thresholds were observed in iAMD and RPD eyes at loci with normal scotopic thresholds; the opposite was rarely encountered. Elevated sCDD thresholds were also observed in iAMD eyes with normal DA. Elevated sCDD thresholds were associated with increased age and presence of late AMD in the fellow eye. The optimal machine learning model predicted 16% of variability (cross-validated R2) in sCDD thresholds at 8 degrees. Discussion: A novel scotopic contour deformation task can provide unique information about rod dysfunction in participants with iAMD and RPD not observed with structural and other functional assessments. Rod dysfunction observed with scotopic contour deformation testing was associated with factors linked to risk of AMD progression.

Persistent Dark Cones in Oligocone Trichromacy Revealed by Multimodal Adaptive Optics Ophthalmoscopy.

Dark cone photoreceptors, defined as those with diminished or absent reflectivity when observed with adaptive optics (AO) ophthalmoscopy, are increasingly reported in retinal disorders. However, their structural and functional impact remain unclear. Here, we report a 3-year longitudinal study on a patient with oligocone trichromacy (OT) who presented with persistent, widespread dark cones within and near the macula. Diminished electroretinogram (ERG) cone but normal ERG rod responses together with normal color vision confirmed the OT diagnosis. In addition, the patient had normal to near normal visual acuity and retinal sensitivity. Occasional dark gaps in the photoreceptor layer were observed on optical coherence tomography, in agreement with reflectance AO scanning light ophthalmoscopy, which revealed that over 50% of the cones in the fovea were dark, increasing to 74% at 10° eccentricity. In addition, the cone density was 78% lower than normal histologic value at the fovea, and 20-40% lower at eccentricities of 5-15°. Interestingly, color vision testing was near normal at locations where cones were predominantly dark. These findings illustrate how a retina with predominant dark cones that persist over at least 3 years can support near normal central retinal function. Furthermore, this study adds to the growing evidence that cones can continue to survive under non-ideal conditions.

Scotopic contour and shape discrimination using radial frequency patterns.

Radial frequency (RF) patterns are valuable tools for investigations of contour integration and shape discrimination. Under photopic conditions, healthy observers can detect deformations from circularity in RF patterns as small as 3 seconds of arc. Such fine discrimination may be facilitated by cortical curvature detectors or global shape-detecting mechanisms that favor a closed contour. Rods make up 95% of photoreceptors in the retina, but we know very little about how spatial information is processed by rod-mediated pathways. We measured scotopic radial deformation discrimination using both full and partly occluded RF pattern stimuli. We found radial deformation thresholds of around 2-3 minutes of arc for stimuli with a wide range of radii and RFs. When parts of the stimulus were occluded, scotopic thresholds improved up to the point that three or four cycles of modulation were visible; no further improvement occurred with the addition of more visible cycles. When only one to three cycles were visible, an increase in curvature per cycle became important, allowing observers to detect smaller deformations from circularity. Our results indicate that the scotopic radial deformation thresholds for the stimuli tested are not dependent on global circularity cues but are instead mediated by local curvature cues.

Characterization of Rod Function Phenotypes Across a Range of Age-Related Macular Degeneration Severities and Subretinal Drusenoid Deposits.

Purpose: To examine spatial changes in rod-mediated function in relationship to local structural changes across the central retina in eyes with a spectrum of age-related macular degeneration (AMD) disease severity. Methods: Participants were categorized into five AMD severity groups based on fundus features. Scotopic thresholds were measured at 14 loci spanning ±18° along the vertical meridian from one eye of each of 42 participants (mean = 71.7 ± 9.9 years). Following a 30% bleach, dark adaptation was measured at eight loci (±12°). Rod intercept time (RIT) was defined from the time to detect a -3.1 log cd/m2 stimulus. RITslope was defined from the linear fit of RIT with decreasing retinal eccentricity. The presence of subretinal drusenoid deposits (SDD), ellipsoid (EZ) band disruption, and drusen at the test loci was evaluated using optical coherence tomography. Results: Scotopic thresholds indicated greater rod function loss in the macula, which correlated with increasing AMD group severity. RITslope, which captures the spatial change in the rate of dark adaptation, increased with AMD severity (P < 0.0001). Three rod function phenotypes emerged: RF1, normal rod function; RF2, normal scotopic thresholds but slowed dark adaptation; and RF3, elevated scotopic thresholds with slowed dark adaptation. Dark adaptation was slowed at all loci with SDD or EZ band disruption, and at 32% of loci with no local structural changes. Conclusions: Three rod function phenotypes were defined from combined measurement of scotopic threshold and dark adaptation. Spatial changes in dark adaptation across the macula were captured with RITslope, which may be a useful outcome measure for functional studies of AMD.

The Perpetual Diamond: Contrast Reversals Along Thin Edges Create the Appearance of Motion in Objects.

The Perpetual Diamond produces motion continuously and unambiguously in one direction despite never physically changing location. The phenomenon consists of a steady, mid-luminance diamond bordered by four thin edge strips and a surrounding background field. The direction of motion is determined by the relative phases of the luminance modulation between the edge strips and the background. Because the motion is generated entirely by changing contrast signals between the edge strips and background, the stimulus is a valuable tool for tests of spatial contrast, temporal contrast, contrast gain, and color contrast. We demonstrate that observers see motion even when the edge strips subtend only seconds of arc on the retina (which is less than the frequently reported 10 minutes of arc) and that perceived motion is due entirely to changes in the difference in contrast phase modulation, independent from the luminance phase.

The separation of monocular and binocular contrast.

The contrast asynchrony is a stimulus configuration that illustrates the visual system's separable responses to luminance and luminance contrast information (Shapiro, 2008; Shapiro et al., 2004). When two disks, whose luminances modulate in phase with each other, are each surrounded by a disk, one light and one dark, observers can see both the in-phase brightness signals and the antiphase contrast signals and can separate the two. Here we present the results of experiments in which observers viewed a similar stimulus dichoptically. We report that no asynchrony is perceived when one eye is presented with modulating disks and the other eye is presented with the black and white surround rings, nor is an asynchrony perceived in gradient versions of the contrast asynchrony. We also explore the "window shade illusion" (Shapiro, Charles, & Shear-Heyman, 2005) dichoptically and find that when a modulating disk is presented to one eye and a horizontally split black/white annulus is presented to the other, observers perceive a "shading" motion up and down the disk. This shading can be seen in either direction in the binocular condition, but it is almost always seen as moving towards low contrast in the monocular condition. These findings indicate the presence of separable retinal and cortical networks for contrast processing at different temporal and spatial scales.