Investigating multi-material hydrogel three-dimensional printing for in vitro representation of the neo-vasculature of solid tumours: a comprehensive mechanical analysis and assessment of nitric oxide release from human umbilical vein endothelial cells.

Many solid tumours (e.g. sarcoma, carcinoma and lymphoma) form a disorganized neo-vasculature that initiates uncontrolled vessel formation to support tumour growth. The complexity of these environments poses a significant challenge for tumour medicine research. While animal models are commonly used to address some of these challenges, they are time-consuming and raise ethical concerns. In vitro microphysiological systems have been explored as an alternative, but their production typically requires multi-step lithographic processes that limit their production. In this work, a novel approach to rapidly develop multi-material tissue-mimicking, cell-compatible platforms able to represent the complexity of a solid tumour's neo-vasculature is investigated via stereolithography three-dimensional printing. To do so, a series of acrylate resins that yield covalently photo-cross-linked hydrogels with healthy and diseased mechano-acoustic tissue-mimicking properties are designed and characterized. The potential viability of these materials to displace animal testing in preclinical research is assessed by studying the morphology, actin expression, focal adhesions and nitric oxide release of human umbilical vein endothelial cells. These materials are exploited to produce a simplified multi-material three-dimensional printed model of the neo-vasculature of a solid tumour, demonstrating the potential of our approach to replicate the complexity of solid tumours in vitro without the need for animal testing.

Automated Real-Time Eddy Current Array Inspection of Nuclear Assets.

Inspection of components with surface discontinuities is an area that volumetric Non-Destructive Testing (NDT) methods, such as ultrasonic and radiographic, struggle in detection and characterisation. This coupled with the industrial desire to detect surface-breaking defects of components at the point of manufacture and/or maintenance, to increase design lifetime and further embed sustainability in their business models, is driving the increased adoption of Eddy Current Testing (ECT). Moreover, as businesses move toward Industry 4.0, demand for robotic delivery of NDT has grown. In this work, the authors present the novel implementation and use of a flexible robotic cell to deliver an eddy current array to inspect stress corrosion cracking on a nuclear canister made from 1.4404 stainless steel. Three 180-degree scans at different heights on one side of the canister were performed, and the acquired impedance data were vertically stitched together to show the full extent of the cracking. Axial and transversal datasets, corresponding to the transmit/receive coil configurations of the array elements, were simultaneously acquired at transmission frequencies 250, 300, 400, and 450 kHz and allowed for the generation of several impedance C-scan images. The variation in the lift-off of the eddy current array was innovatively minimised through the use of a force-torque sensor, a padded flexible ECT array and a PI control system. Through the use of bespoke software, the impedance data were logged in real-time (≤7 ms), displayed to the user, saved to a binary file, and flexibly post-processed via phase-rotation and mixing of the impedance data of different frequency and coil configuration channels. Phase rotation alone demonstrated an average increase in Signal to Noise Ratio (SNR) of 4.53 decibels across all datasets acquired, while a selective sum and average mixing technique was shown to increase the SNR by an average of 1.19 decibels. The results show how robotic delivery of eddy current arrays, and innovative post-processing, can allow for repeatable and flexible surface inspection, suitable for the challenges faced in many quality-focused industries.