RESUMO
Spectroscopy and machine learning (ML) algorithms have provided significant advances to the modern food industry. Instruments focusing on near-infrared spectroscopy allow obtaining information about seed and grain chemical composition, which can be related to changes caused by field pesticides. We investigated the potential of FT-NIR spectroscopy combined with Linear Discriminant Analysis (LDA) to discriminate chickpea seeds produced using different desiccant herbicides at harvest anticipation. Five herbicides applied at three moments of the plant reproductive stage were utilized. The NIR spectra obtained from individual seeds were used to build ML models based on LDA algorithm. The models developed to identify the herbicide and the plant phenological stage at which it was applied reached 94% in the independent validation set. Thus, the LDA models developed using near-infrared spectral data provided to be efficient, quick, non-destructive, and accurate to identify differences between seeds due to pre-harvest herbicides application.
Assuntos
Cicer/embriologia , Sementes/classificação , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Algoritmos , Análise Discriminante , Grão Comestível , Análise de Fourier , Sementes/químicaRESUMO
A modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method was validated and applied for the estimation of half-lives of two imidazolinone group herbicides, imazethapyr and imazapic, in the Dystric Plinthosol (FX) and Ferralsol (LVA) agricultural soils using liquid chromatography tandem mass spectrometry. The solutions were prepared in a matrix extract to avoid matrix effects. The analytical method showed satisfactory specificity, selectivity, linearity (R2 > 0.99), recoveries (range 85.0-117.0%), with RSD between 6.7% and 16.6%, and precision (range 94.7-108.5%), with RSD between 6.0% and 11.9%. The limit of detections for imazethapyr and imazapic in the soils were 2.2⯵gâ¯kg-1 and 2.0⯵gâ¯kg-1, and the limit of quantifications were 6.6⯵gâ¯kg-1 and 6.1⯵gâ¯kg-1. The half-lives of imazethapyr (35.7 and 97.9 days) and imazapic (40.4 and 64.4 days), in the FX and LVA soils, respectively, indicate that they are medium-persistence herbicides with possibility of leaching into groundwater. In addition, high concentrations of imazethapyr and imazapic were found in the soil samples after the time of application, meaning that there is a potential for prolonged soil residual activity due to carryover.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Herbicidas/análise , Imidazóis/análise , Ácidos Nicotínicos/análise , Poluentes do Solo/análise , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida/métodos , Meia-Vida , Sensibilidade e Especificidade , Solo/químicaRESUMO
ABSTRACT: The application of desiccant herbicides in the bean crop is fundamental in the production of quality seeds, since it anticipates the harvesting season, which makes it possible to obtain seeds in the period of physiological maturation, when they are reported with maximum accumulation of dry matter, high vigor levels and germination percentage. However, there is little information about the ideal application time of these products to obtain beans of high physiological quality. Therefore, the objective of this study was to evaluate the physiological quality of bean seeds after application of desiccant herbicides at different times in preharvest. The experimental design was a randomized complete block design in a 2x3+1 factorial scheme, with four replications. Desiccant herbicides paraquat (400g ha-1) and paraquat + diuron (200 + 400g ha-1) were applied in three phenological stages (R8, R8/R9 and R9), plus one control (without herbicide application). Yield (Kg ha-1), mass of one thousand seeds (g), germination (%) and seed vigor were evaluated through tests of accelerated aging, electrical conductivity and primary root length. Application of paraquat molecule at the R8 stage and the paraquat + diuron mixture at the R8/R9 stage reduced the viability and vigor of the bean seeds, and compromised yield. Applications of the paraquat herbicide at the R8/R9 and R9 stages or of the mixture (paraquat + diuron) at the R9 stage preserved the physiological quality of the seeds without;however, affecting yield.
RESUMO: A aplicação de herbicidas dessecantes na cultura do feijoeiro é fundamental na produção de sementes de qualidade porque antecipa a época de colheita, o que possibilita a obtenção de sementes no período de maturação fisiológica, quando se encontram com máximo acúmulo de matéria seca, elevados níveis de vigor e alta porcentagem de germinação. Todavia, são escassas as informações acerca da época ideal de aplicação desses produtos na obtenção de sementes de feijão de alta qualidade fisiológica. Por essa razão, objetivou-se avaliar a qualidade fisiológica de sementes de feijão após aplicações de herbicidas dessecantes em diferentes épocas na pré-colheita. O delineamento experimental utilizado foi o de blocos casualizados dispostos em esquema fatorial 2x3+1, com quatro repetições. A aplicação dos herbicidas dessecantes paraquat (400g ha-1) e paraquat + diuron (200+400g ha-1) foi realizada em três estádios fenológicos (R8, R8/R9 e R9), mais uma testemunha (sem aplicação do herbicida). Avaliou-se a produtividade (Kg ha-1), a massa de mil sementes (g), a germinação (%) e o vigor das sementes por meio dos testes de envelhecimento acelerado, condutividade elétrica e comprimento da raiz primária. A aplicação da molécula de paraquat no estádio R8 e da mistura paraquat + diuron no estágio R8/R9 reduziu a viabilidade e o vigor das sementes de feijão, além disso comprometeu a produtividade. Aplicações dos herbicidas paraquat nos estádios R8/R9 e R9 ou da mistura (paraquat + diuron) no estádio R9 preservaram a qualidade fisiológica das sementes, sem, entretanto, afetar a produtividade.
RESUMO
O objetivo do trabalho foi avaliar o crescimento de feijão-caupi sob o efeito de veranico nos sistemas de plantio direto e convencional. Para isso, foi realizado um experimento na Universidade Federal Rural do Semiárido, utilizando-se a cultivar de feijão-caupi BRS Guariba em plantio direto e convencional. Para aplicação do veranico, a irrigação foi mantida em parte dos tratamentos e cortada em outros por 22 dias, sendo os tratamentos iniciados aos 34 dias após a semeadura, ocasião em que foi verificado o início do florescimento. Entre os 15 e 64 dias após a semeadura foram realizadas, semanalmente, coletas de plantas para avaliação. Os sistemas de plantio influenciam o crescimento da área foliar, da matéria seca do caule, de folhas, de vagens e total do feijão-caupi. Os índices de crescimento foram influenciados pela fase fenológica da planta (idade) e pelo estresse hídrico. As maiores taxas de crescimento foram alcançadas no sistema de plantio direto. O estresse hídrico, afeta negativamente todas as variáveis avaliadas, independentemente do sistema de plantio.
The objective of this study was to evaluate the growth of cowpea under the influence of dry spell in no-tillage and conventional crop systems. For this purpose an experiment was conducted at the Universidade Federal Rural do Semi-Arido, using the cowpea cultivar BRS Guariba in both crop systems. For the dry spells simulation, irrigation was suspended for 22 days, with the treatments starting at 34 days after sowing, at the onset of flowering. Between 15 and 64 days after sowing plants samples were collected weekly to evaluate. The tillage systems influenced the growth of leaf area, and the dry matter of stem, leaf, pod and plant. The growth rates were influenced by the plant phenological phase (age) and by water stress. The highest growth rates were achieved in the no-tillage system. Water stress affects negatively all the variables studied, regardless of tillage system.