"It Takes a Virtual Village" Achieving Magnet Redesignation Amidst the COVID-19 Pandemic: Canada's Magnet Hospital Journey.

This article outlines how a Canadian hospital achieved the American Nursing Credentialing Center Magnet Recognition Program redesignation after participating in a virtual site visit (VSV) appraisal process amidst the COVID-19 pandemic. Within our current COVID-19 landscape, being a resilient Magnet-designated organization is paramount. In this context, the American Nurses Credentialing Center (ANCC) has developed a VSV model that (1) extends the use of audio/video (A/V) conferencing technology to showcase nursing excellence; (2) maintains the integrity of the appraisal process; and (3) ensures the safety and well-being of staff, patients and their care partners, and the appraisers. Key narrative insights are highlighted around planning and on-site execution of a successful VSV. The redesignation is a culmination of several stakeholders' efforts who shared their sense of pride, inspiration, and accomplishment during the VSV. The redesignation status notification exemplifies resiliency and was welcomed amidst uncertainty with the evolving COVID-19 pandemic. The planning and on-site implementation plan may serve as a blueprint for others who will be engaged in a VSV as part of their designation or redesignation journey. Insights are shared around preparing for the VSV, hosting the VSV, and achieving the ANCC Magnet Recognition Program redesignation.

Ni-Catalyzed ß-Alkylation of Cyclopropanol-Derived Homoenolates.

Metal homoenolates are valuable synthetic intermediates which provide access to ß-functionalized ketones. In this report, we disclose a Ni-catalyzed ß-alkylation reaction of cyclopropanol-derived homoenolates using redox-active N-hydroxyphthalimide (NHPI) esters as the alkylating reagents. The reaction is compatible with 1°, 2°, and 3° NHPI esters. Mechanistic studies imply radical activation of the NHPI ester and 2e ß-carbon elimination occurring on the cyclopropanol.

Coupling of pollination services and coffee suitability under climate change.

Climate change will cause geographic range shifts for pollinators and major crops, with global implications for food security and rural livelihoods. However, little is known about the potential for coupled impacts of climate change on pollinators and crops. Coffee production exemplifies this issue, because large losses in areas suitable for coffee production have been projected due to climate change and because coffee production is dependent on bee pollination. We modeled the potential distributions of coffee and coffee pollinators under current and future climates in Latin America to understand whether future coffee-suitable areas will also be suitable for pollinators. Our results suggest that coffee-suitable areas will be reduced 73-88% by 2050 across warming scenarios, a decline 46-76% greater than estimated by global assessments. Mean bee richness will decline 8-18% within future coffee-suitable areas, but all are predicted to contain at least 5 bee species, and 46-59% of future coffee-suitable areas will contain 10 or more species. In our models, coffee suitability and bee richness each increase (i.e., positive coupling) in 10-22% of future coffee-suitable areas. Diminished coffee suitability and bee richness (i.e., negative coupling), however, occur in 34-51% of other areas. Finally, in 31-33% of the future coffee distribution areas, bee richness decreases and coffee suitability increases. Assessing coupled effects of climate change on crop suitability and pollination can help target appropriate management practices, including forest conservation, shade adjustment, crop rotation, or status quo, in different regions.

Exploiting post-transcriptional regulation to probe RNA structures in vivo via fluorescence.

While RNA structures have been extensively characterized in vitro, very few techniques exist to probe RNA structures inside cells. Here, we have exploited mechanisms of post-transcriptional regulation to synthesize fluorescence-based probes that assay RNA structures in vivo. Our probing system involves the co-expression of two constructs: (i) a target RNA and (ii) a reporter containing a probe complementary to a region in the target RNA attached to an RBS-sequestering hairpin and fused to a sequence encoding the green fluorescent protein (GFP). When a region of the target RNA is accessible, the area can interact with its complementary probe, resulting in fluorescence. By using this system, we observed varied patterns of structural accessibility along the length of the Tetrahymena group I intron. We performed in vivo DMS footprinting which, along with previous footprinting studies, helped to explain our probing results. Additionally, this novel approach represents a valuable tool to differentiate between RNA variants and to detect structural changes caused by subtle mutations. Our results capture some differences from traditional footprinting assays that could suggest that probing in vivo via oligonucleotide hybridization facilitates the detection of folding intermediates. Importantly, our data indicate that intracellular oligonucleotide probing can be a powerful complement to existing RNA structural probing methods.