RESUMO
Purpose: To quantify relevant fundus autofluorescence (FAF) image features cross-sectionally and longitudinally in a large cohort of inherited retinal diseases (IRDs) patients. Design: Retrospective study of imaging data (55-degree blue-FAF on Heidelberg Spectralis) from patients. Participants: Patients with a clinical and molecularly confirmed diagnosis of IRD who have undergone FAF 55-degree imaging at Moorfields Eye Hospital (MEH) and the Royal Liverpool Hospital (RLH) between 2004 and 2019. Methods: Five FAF features of interest were defined: vessels, optic disc, perimacular ring of increased signal (ring), relative hypo-autofluorescence (hypo-AF) and hyper-autofluorescence (hyper-AF). Features were manually annotated by six graders in a subset of patients based on a defined grading protocol to produce segmentation masks to train an AI model, AIRDetect, which was then applied to the entire imaging dataset. Main Outcome Measures: Quantitative FAF imaging features including area in mm 2 and vessel metrics, were analysed cross-sectionally by gene and age, and longitudinally to determine rate of progression. AIRDetect feature segmentation and detection were validated with Dice score and precision/recall, respectively. Results: A total of 45,749 FAF images from 3,606 IRD patients from MEH covering 170 genes were automatically segmented using AIRDetect. Model-grader Dice scores for disc, hypo-AF, hyper-AF, ring and vessels were respectively 0.86, 0.72, 0.69, 0.68 and 0.65. The five genes with the largest hypo-AF areas were CHM , ABCC6 , ABCA4 , RDH12 , and RPE65 , with mean per-patient areas of 41.5, 30.0, 21.9, 21.4, and 15.1 mm 2 . The five genes with the largest hyper-AF areas were BEST1 , CDH23 , RDH12 , MYO7A , and NR2E3 , with mean areas of 0.49, 0.45, 0.44, 0.39, and 0.34 mm 2 respectively. The five genes with largest ring areas were CDH23 , NR2E3 , CRX , EYS and MYO7A, with mean areas of 3.63, 3.32, 2.84, 2.39, and 2.16 mm 2 . Vessel density was found to be highest in EFEMP1 , BEST1 , TIMP3 , RS1 , and PRPH2 (10.6%, 10.3%, 9.8%, 9.7%, 8.9%) and was lower in Retinitis Pigmentosa (RP) and Leber Congenital Amaurosis genes. Longitudinal analysis of decreasing ring area in four RP genes ( RPGR, USH2A, RHO, EYS ) found EYS to be the fastest progressor at -0.18 mm 2 /year. Conclusions: We have conducted the first large-scale cross-sectional and longitudinal quantitative analysis of FAF features across a diverse range of IRDs using a novel AI approach.
RESUMO
A una muestra de 873 trabajadores de organizaciones públicas de Argentina, se ha administrado el Cuestionario de Evaluación de Factores Psicosociales (CEFAP) que incluye once dimensiones, con una serie de ítems con formato tipo Likert organizados de acuerdo con un modelo teórico según el cual éstos cubrirían el espectro de factores psicosociales que contextualizan el trabajo. Todos los factores mostraron una alta consistencia interna, con valores α de Cronbach comprendidos entre .49 (Relación salud-trabajo) y .92 (Apoyo Social y Estima). Además, el cuestionario cuenta con un apartado donde se relevan variables sociolaborales. Los resultados indican que el CEFAP reproduce fielmente la estructura del modelo teórico propuesto ampliando lo presentado por otros instrumentos de evaluación.
The Questionnaire for the Evaluation of Psychosocial Factors (CEFAP) was administered to a sample of 873 employees of public organizations of Argentina. The questionnaire includes eleven dimensions, with a series of items Likert-formatted organized according to a theoretical model that states that these would cover the spectrum of psychosocial factors that contextualize the working activity. All factors showed high internal consistency with Cronbach α values ranging from .49 (Health-work relationship) and .92 (Social Support and Esteem). In addition, the questionnaire has a section where occupational variables are collected. The results indicate that the CEFAP faithfully reproduces the structure of the proposed theoretical model, expanding what was presented by other assessment tools.