The impact of COVID-19 infection on labor outcomes of Mexican formal workers.

The outbreak of the COVID-19 pandemic had an immediate and severe impact on the economy. However, we do not know whether the disease may have a longer-term effect on people's employment opportunities. In this study, we focus on the effects of COVID-19 infection on labor market outcomes 12 months after diagnosis. We use a unique dataset that includes all formal private sector workers in the Mexican social security system and that links health outcomes with administrative records. We implement two alternative identification strategies to estimate the impact: matching estimators and individual fixed effects models. Our study finds that COVID-19 infection does not harm employment probabilities or wages. On the contrary, we find that workers who had tested positive for COVID had a higher likelihood of keeping their formal sector jobs and higher wages than those who did not. Moreover, our results describe mostly low-income workers.

Hydrostatic Penetration Testing of Protective Glove Materials Using Water and Synthetic Blood to Evaluate Hole Size and Screen Mesh Using an Automated Pressure Delivery System.

There are several international standards that address the resistance of chemical protective clothing materials to the penetration by liquids. The hydrostatic pressure has been documented to discriminate between protective clothing material performance and correlates with visual penetration results that are obtained with human factors validation. The same methodology, based on hydrostatic pressure equipment, is referenced also in other standards addressing penetration resistance of protective clothing and glove materials against synthetic blood or blood-borne pathogens. In this study, we present an automated hydrostatic penetration testing that integrates testing procedures from several standards to evaluate the resistance of materials to penetration by liquids under pressure. The automated control system allows the user to select a specific test method and automatically sets a stepped pressurization protocol to test the material. A pass or a fail result is produced at a certain time and pressure. As an example of application, the penetration of synthetic blood was assessed through gloves made from different materials with ISO 16603, method B, one of the five possible penetration methods and protocols available in the test equipment. The results indicate that the developed system facilitates the application of test methods used to evaluate the barrier effectiveness against liquids of materials used for protective clothing and gloves and show up that the characteristics of the retention grid used have a decisive influence on the test results. In some of the tested glove materials, holes were intentionally performed with needles with different gauges. The capacity of pinhole detection in gloves was evaluated according to the test method selected and compared with results obtained with the classic water leak test method for gloves described in EN ISO 374-2.

Why Do School Staff Sometimes Fail to Report Potential Victimization Cases? A Mixed-methods Study.

Schools are in a position to connect children and adolescents suspected of being victims of violence with an external source of support by making referrals to external agencies. However, several studies have identified obstacles that hinder early reporting among school staff members. Very few studies have applied a mixed-method approach to try to understand this sensitive issue. The current study used this approach to analyze to what extent the students suspected of being victimized match the ones reported by active school staff members in Spain (n = 453, 83.5% females, age: M = 42.23, SD = 9.46). We classified the reasons given for not reporting the potential victimization cases encountered and made comparisons to determine whether there were differences in the level of knowledge, or in the sociodemographic characteristics, of respondents who gave different reasons for not reporting. Although 73.5% of school staff members had detected at least one potential case, 40.8% of them referred it to an external agency. The most common reasons for lack of reporting included deciding not to do so once concerns had been shared within the school and believing that one must be certain or that only serious violence should be reported. The findings of this study may help to further understand the decisions not to report certain suspicions of potential victimization cases to external agencies by school staff. There is an urgent need to raise awareness about the duty to report these concerns to external agencies, even in the absence of agreement from the school management team. Members of school staff need to be strongly encouraged to become familiar with the existing protocols.

The income gradient in COVID-19 mortality and hospitalisation: An observational study with social security administrative records in Mexico.

BACKGROUND: The COVID-19 pandemic revealed large structural inequalities that led to disparities in health outcomes related to socioeconomic status. So far, most of the evidence is based on aggregated data or simulations with individual data, which point to various possible mechanisms behind the association. To date, there have been no studies regarding an income gradient in COVID-19 mortality based on individual-level data and adjusting for comorbidities or access to healthcare. METHODS: In this paper, we use linked employee-patient data for patients tested for COVID-19 at the Mexican Institute of Social Security. We estimate the association of the probability of dying with income centiles, using a probit estimation and adjusting for COVID-19 diagnosis, sociodemographic variables, and comorbidities. FINDINGS: After controlling for all these variables, we find that persons in the lowest income decile still had a probability of dying from COVID-19 five times greater than those at the top decile. INTERPRETATION: Our results imply that the association between income and COVID outcomes is not explained by the prevalence of comorbidities or by a lack of access to healthcare among the low-income population. FUNDING: This study was not supported by any external funding source.

Distribution Levels of Particulate Matter Fractions (<2.5 µm, 2.5-10 µm and >10 µm) at Seven Primary Schools in a European Ceramic Cluster.

This study addresses the concentration of particulate matter and their size using a statistical analysis of data obtained inside seven schools located in the towns of Castellón (S1, S2, and S3), Alcora (S4, S5, and S6) and Lucena (S7) in northeast Spain. Samples were taken for five to eight hours, depending on school hours, to obtain a monthly sample for each school. The main goal of this study is to assess the differences depending on the type of location and the sampling point to be able to design corrective measures that improve the habitability and safety of the teaching spaces analyzed. The lowest concentrations of fine particulate matter, less than 2.5 µm, were registered at the rural location. The values of these particles found in industrial and urban locations were not substantially different. In the case of particulate matter between 2.5 and 10 µm, significant differences were observed between the three types of locations. The lowest concentrations of particles larger than 10 µm were registered at the rural location, and the highest concentrations were found at the industrial locations. Among the urban stations, the particle concentration of this fraction in station S2 was significantly higher than that in stations S1 and S3, which had similar concentrations. These values are also similar to those registered at school S6, which is at an industrial location. The resuspension of particles from both indoor sources as well as those transported from the outside is an important factor in the concentrations of particles inside classrooms.

Gene Expression Analysis of Induced Plum pox virus (Sharka) Resistance in Peach (Prunus persica) by Almond (P. dulcis) Grafting.

No natural sources of resistance to Plum pox virus (PPV, sharka disease) have been identified in peach. However, previous studies have demonstrated that grafting a "Garrigues" almond scion onto "GF305" peach rootstock seedlings heavily infected with PPV can progressively reduce disease symptoms and virus accumulation. Furthermore, grafting a "Garrigues" scion onto the "GF305" rootstock has been shown to completely prevent virus infection. This study aims to analyse the rewiring of gene expression associated with this resistance to PPV transmitted by grafting through the phloem using RNA-Seq and RT-qPCR analysis. A total of 18 candidate genes were differentially expressed after grafting "Garrigues" almond onto healthy "GF305" peach. Among the up-regulated genes, a HEN1 homolog stands out, which, together with the differential expression of RDR- and DCL2-homologs, suggests that the RNA silencing machinery is activated by PPV infection and can contribute to the resistance induced by "Garrigues" almond. Glucan endo-1,3-beta D-glucosidase could be also relevant for the "Garrigues"-induced response, since its expression is much higher in "Garrigues" than in "GF305". We also discuss the potential relevance of the following in PPV infection and "Garrigues"-induced resistance: several pathogenesis-related proteins; no apical meristem proteins; the transcription initiation factor, TFIIB; the speckle-type POZ protein; in addition to a number of proteins involved in phytohormone signalling.

Morphological Characterization of Indoor Airborne Particles in Seven Primary Schools.

This paper focuses on a study of fine (less than 2.5 µm) airborne particles collected inside seven primary schools located on a coastal Mediterranean area which has a significant industrial hub for the processing of clays and other minerals as raw materials. Of the seven schools, three are located in an urban area 20 km away from the main industrial hub, three are in a town located next to the industrial estate, and one is in a rural location, 30 km from the industrial area. The objective of this study is to identify the main types of particles found in the three examined environments. The particle matter identified in the fine particle samples is grouped into three main groups: mineral compounds, particles from combustion processes and phases emitted in high-temperature industrial processes. The mineral particles, which can come from natural or anthropogenic emissions, have been classified depending on their morphology as isometric allotriomorphs or subidiomorphs, with a tabular habit; acicular forms; or pure crystalline forms. Compounds from combustion processes have two types of morphologies: spheroid and dendritic soot particles. Additionally, in smaller quantities, spherical particles associated with high-temperature industrial processes, such as the emissions of ceramic tile-firing and frit-melting processes, are identified. A summary table is shown, which lists the characteristics, as well as the most significant origin of the main particles identified in the fine (<2.5 µm) airborne particles collected inside primary schools located in three different environments (urban, industrial and rural). A visual scale has been established based on the number of particles observed in the samples of the atmospheric particulate fraction between the sizes of 2.5 and 10 µm collected inside the schools. The ratios Ca/Si, S/Si, S/Ca and (Si+Ca)/S have been established. Results obtained may be useful in epidemiological studies in the ceramic cluster area in order to estimate children's exposure to different indoor primary school microenvironments. Effective policies and mitigation measurements for the protection of children's health should be carried out in this highly industrialized area.

Thoughts About Real Nursing.

This essay presents thoughts about what constitutes real nursing and who may be considered a real nurse.

Estado nutricional en pacientes con cáncer cervico-uterino al ingreso hospitalario / Nutritional status in patients with cervical cancer at hospital admission

RESUMEN Existe un gran aumento de la frecuencia de desnutrición en pacientes con cáncer, sin embargo, la información disponible sobre la evaluación del estado de nutrición en pacientes con cáncer cervicouterino (CaCu) es limitada. Material y métodos: Estudio retrospectivo, observacional, transversal. Se realizó una revisión de expedientes clínicos, en los cuales, el estado nutricional fue evaluado con una valoración nutricional institucional que incluye parámetros antropométricos (peso, IMC, pliegue cutáneo tricipital (PCT), circunferencia media de brazo (CMB), % masa grasa), bioquímicos (albúmina, hemoglobina, linfocitos) y dietéticos (consumo energético y proteico). Resultados: Se incluyeron 195 expedientes de pacientes con CaCu. La frecuencia de los estadios tempranos fue de 51.8% y en esas pacientes existió una prevalencia de desnutrición determinada por la albúmina de 9.75% y de 25.13 % de acuerdo a los linfocitos, incrementándose a 41.18% de desnutrición de acuerdo al PCT en estadios avanzados. Se encontraron correlaciones estadísticamente significativas entre el estadio clínico y la ingesta calórica (r= −0.3062 p< 0.0001), la concentración de albúmina (r= −0.4732 p< 0.0001) y la circunferencia media de brazo (r= −0.2564 p= 0.0003). Conclusión: El estado nutricional en estadios tempranos del CaCu no presenta alteraciones, mientras que en estadios intermedios y avanzados la alteración del estado nutricional aumenta.

ABSTRACT There is a high frequency of malnutrition in cancer patients; however, available information on the assessment of nutritional status in patients with cervical cancer is limited. Material and methods: We conducted a retrospective, observational, cross-sectional study. Clinical records were reviewed; nutritional status was evaluated with the institutional nutritional evaluation tool that includes anthropometric (weight, BMI, tricipital skin fold, medium arm circumference, fat mass %), biochemical (albumin, hemoglobin, lymphocytes) and diet parameters (energy and protein consumption). Results: We included the records of 195 patients with cervical cancer. The frequency of the early stage cancer was 51.8% and in those patients there was a prevalence of malnutrition, determined by albumin, of 9.75% and 25.13% according to the lymphocytes, increasing to 41.18% measured by tricipital skin fold among patients with advanced-stage cancer. Statistically significant correlations were found between clinical stage and caloric intake (r= −0.3062 p< 0.0001), albumin concentration (r= −0.4732 p <0.0001) and mean arm circumference (r = −0.2564 p= 0.0003). Conclusion: The nutritional status in early stages of CaCu does not present alterations, while in intermediate and advanced stages the alteration of nutritional status may increase.

Helping High School Students Explore Nursing Careers in a Summer Internship Program.

Although nursing remains the most trusted profession in the United States, it is still challenging to attract high school students due to a perception that nursing may not be as intellectual, challenging, or prestigious as other careers in health care. Nursing professional development practitioners can create an opportunity to change this perception by engaging high school students through a summer internship program. The Student Career Opportunity Outreach Program embeds high school students in the hospital environment, enabling them to be a part of a clinical area and engage with nurses in a meaningful way. This article aims to explain the components of the summer internship program for high school students in a pediatric academic medical center and discuss findings from a survey exploring career choices pre- and postprogram as well as other outcome measures.

Endometrial receptivity in eutopic endometrium in patients with endometriosis: it is not affected, and let me show you why.

Many women with endometriosis experience compromised fertility. This disease clearly exerts quantitative damage on the ovaries, and perhaps, also qualitative damage. However, it remains controversial whether endometrial receptivity is compromised. Here we review the evidence from basic transcriptomic signature data to clinical data from an oocyte donation model and find support for the concept that endometrial receptivity is not impaired in women with endometriosis when healthy embryos reach the endometrial cavity.

Deforestation Impacts on Bat Functional Diversity in Tropical Landscapes.

Functional diversity is the variability in the functional roles carried out by species within ecosystems. Changes in the environment can affect this component of biodiversity and can, in turn, affect different processes, including some ecosystem services. This study aimed to determine the effect of forest loss on species richness, abundance and functional diversity of Neotropical bats. To this end, we identified six landscapes with increasing loss of forest cover in the Huasteca region of the state of Hidalgo, Mexico. We captured bats in each landscape using mist nets, and calculated functional diversity indices (functional richness and functional evenness) along with species richness and abundance. We analyzed these measures in terms of percent forest cover. We captured 906 bats (Phyllostomidae and Mormoopidae), including 10 genera and 12 species. Species richness, abundance and functional richness per night are positively related with forest cover. Generalized linear models show that species richness, abundance and functional richness per night are significantly related with forest cover, while seasonality had an effect on abundance and functional richness. Neither forest cover nor season had a significant effect on functional evenness. All these findings were consistent across three spatial scales (1, 3 and 5 km radius around sampling sites). The decrease in species, abundance and functional richness of bats with forest loss may have implications for the ecological processes they carry out such as seed dispersal, pollination and insect predation, among others.

EARLY: a pilot study on early diagnosis of atrial fibrillation in a primary healthcare centre.

AIM: Atrial fibrillation (AF) is associated with high morbidity and mortality. Early diagnosis is likely to improve therapy and prognosis. The study objective was to evaluate the usefulness of a programme for early diagnosis of AF in patients from an urban primary care centre. METHODS AND RESULTS: Participants were recruited from a randomized sample of patients not diagnosed with AF but having relevant risk factors: age ≥ 65 years, ischaemic and/or valvular heart disease, congestive heart failure, hypertension, and/or diabetes. Patients were randomly assigned to the intervention group (IG) or control group (CG). The intervention included (i) initial visit with clinical history, electrocardiogram, and instruction about pulse palpation and warning signs and (ii) electrocardiogram every 6 months during a 2-year follow-up. The main endpoint of the study was the proportion of new cases diagnosed at 6 months. Secondary endpoints were number of new AF diagnoses and complications associated with the arrhythmia in both groups. A total of 928 patients were included (463 IG and 465 CG). At 6 months, AF was diagnosed in 8 IG patients and 1 CG patient (1.7 vs. 0.2%, respectively, P = 0.018). After 2 years of follow-up, 11 IG patients and 6 CG patients had newly diagnosed AF (2.5 vs. 1.3%, respectively, P = 0.132). Time to first diagnosis of AF was shorter in IG patients [median (inter-quartile range): 7 (192) days vs. 227 (188.5) days in CG, P = 0.029]. CONCLUSION: The simple screening proposed could be useful for the early detection of AF in primary care.

Postoperative vesicoureteral reflux after high-pressure balloon dilation of the ureterovesical junction in primary obstructive megaureter. Incidence, management and predisposing factors.

OBJECTIVE: To describe the incidence, predisposing factors and management of postoperative vesicoureteral reflux (VUR) after high-pressure balloon dilation to treat primary obstructive megaureter (POM). MATERIALS AND METHODS: We have reviewed patients that underwent endoscopic treatment for POM from May 2008 to November 2013. All patients were evaluated with renal ultrasound, voiding cystourethrography and diuretic renogram. Endoscopic treatment was done with high-pressure balloon dilation of the ureterovesical junction under general anesthesia; a double-J stenting was done in all patients. Follow-up was performed with ultrasonography, voiding cystourethrography and a diuretic renogram in all patients. RESULTS: Fifteen boys and five girls with a mean age of 14.18 months (3-103) were reviewed. A total of 22 ureters underwent HPBD to treat POM. Ureterohydronephrosis improves in 19 ureters. After endoscopic treatment, six ureters developed VUR. Four ureters were managed surgically, and in the other two, VUR disappeared in a second cystogram. The presence of parameatal diverticulum in the preoperative cystography and those patients with bilateral POM are factors related to postoperative VUR (p < 0.05). Urinary tract infection after HPBD was observed in four patients, but only one of them was affected with VUR.

Human Endometrial Transcriptomics: Implications for Embryonic Implantation.

Human endometrium has been extensively investigated in the search for markers capable of predicting its receptive status. The completion of the Human Genome Project has triggered a rapid development of new fields in molecular biology, the "transcriptomics" being a major turning point in the knowledge acquisition of endometrial receptivity. Based on this, a customized Endometrial Receptivity Array (ERA) has been developed, which is capable of identifying the genomic signature of receptivity. This diagnostic tool showed that the window of implantation (WOI) is displaced in one out of four patients with implantation failure, allowing the identification of their personalized WOI. This strategy allows performing a personalized embryo transfer (pET) on the day in which the endometrium is receptive. The combination of a systems biology approach and next-generation sequencing will overcome the limitations of microarrays, and will, in the future, allow elucidation of the mechanisms involved in embryo implantation.

Anderson-hynes pyeloplasty in patients less than 12 months old. Is the laparoscopic approach safe and feasible?

PURPOSE: The aim of our study is to compare the outcomes of open and laparoscopic pyeloplasty in children less than 12 months of age. PATIENTS AND METHODS: We reviewed all medical charts of patients less than 12 months old who underwent pyeloplasty from January 2007 to February 2013. We divided them in two groups: Open pyeloplasty (OP) and laparoscopic pyeloplasty (LP). The following data were analyzed: age, sex, weight, US measurements, operative time, hospital stay, complications, and success rate. Quantitative data were analyzed with the Student t test or Mann-Whitney U test, and chi-square test or Fisher test for qualitative data. RESULTS: Fifty-eight patients (46 boys and 12 girls) with a mean age of 4.66 months (±3.05) were included. Mean age was 4.25 months and 5.15 months in OP and LP group respectively. Mean weight was 6.78 kg and 7.02 kg in OP and LP groups. There were no statistical differences in age, weight, and sex between OP and LP groups. There were no statistical differences in preoperative ultrasonography measurements. Mean posterior-anterior (PA) pelvis diameter was 28.57 mm and 23.94 mm in OP and LP groups, respectively. Mean calices diameter were 10.86 mm and 10.96 mm in OP and LP groups, respectively. Mean operative time was 129.53 minutes in the OP group and 151.92 minutes in the LP group with statistical differences (P=0.018). Mean hospital stay was 6.34 days in the OP group and 3.46 in the LP group with statistical differences (P<0.05). No intraoperative and postoperative complications were found in either group. Hydronephrosis improved in all patients, and no patient needed a repeated pyeloplasty. CONCLUSION: The laparoscopic approach of Anderson-Hynes pyeloplasty in patients less than 12 months old is a safe procedure with the same outcomes as the open approach.

The endometrial receptivity array for diagnosis and personalized embryo transfer as a treatment for patients with repeated implantation failure.

OBJECTIVE: To demonstrate the clinical value of the endometrial receptivity array (ERA) in patients with repeated implantation failure (RIF), for guiding their personalized embryo transfer (pET) as a novel therapeutic strategy. DESIGN: Prospective interventional multicenter clinical trial. SETTING: University-affiliated infertility and private clinics. PATIENT(S): Eighty-five RIF patients and 25 comparison patients. INTERVENTION(S): Endometrial sampling and pET guided by ERA. MAIN OUTCOME MEASURE(S): A receptive (R) or nonreceptive (NR) endometrial status according to ERA. Pregnancy (PR) and implantation (IR) rates after pET. RESULT(S): The ERA test gave an R result of 74.1% in RIF patients versus 88% in control subjects. Clinical follow-up was possible in 29 RIF patients, in whom pET was performed, resulting in 51.7% PR and 33.9% IR. The IRs and PRs in the 6 months after the biopsy showed that pregnancy was not related to the local injury. Twenty-two RIF patients (25.9%) were NR, and in 15 of them a second ERA validated a displacement of the window of implantation (WOI). In eight of them, pET was performed on the day designated by the ERA, resulting in 50.0% PR and 38.5% IR. These results should be considered as preliminary. CONCLUSION(S): There is an increased percentage of WOI displacement in RIF patients compared with comparison group patients, leading to the concept of pET as a therapeutic strategy. Rescue of NR patients by pET in a displaced WOI results in similar PR and IR.

Targeted gene delivery to hepatocytes with galactosylated amphiphilic cyclodextrins.

OBJECTIVES: Achieving targeted delivery of gene medicines is desirable to maximise activity. Here, galactosylated amphiphilic cyclodextrins (CDs) are examined in terms of their ability to transfect asialoglycoprotein receptor-bearing HepG2 cells. METHODS: Cationic amphiphilic CDs were synthesised as well as amphiphilic CDs bearing galactose-targeting ligands with different linker lengths. Binding of galactosylated CDs to a galactose-specific lectin was examined by surface plasmon resonance. CDs were formulated with and without the helper lipid DOPE and complexed with plasmid DNA. Transfection was evaluated by luciferase assay. Intracellular trafficking was assessed by confocal microscopy. KEY FINDINGS: Binding of targeted CDs to a galactose-specific lectin was achieved. Binding decreased with linker length between the galactosyl group and the CD core. Contrary to the lectin binding results, transfection levels increased with an increase in linker length from 7 atoms to 15. Compared to non-targeted formulations, a significant increase in transfection was observed only in the presence of the helper lipid DOPE. Confocal microscopy revealed that DOPE caused a pronounced effect on cellular distribution. CONCLUSIONS: The galactose-targeting ligand induced substantial increases in transfection over non-targeted formulations when DOPE was included, indicating the potential for targeted gene delivery using CD-based delivery systems.