RESUMO
Floral nectar contains sugars and numerous other compounds, including amino acids, but little is known about their function and origin in nectar. Therefore, the amino acid, sugar, and inorganic ion concentrations, as well as the activity of alanine aminotransferase (AlaAT) and glutamine synthetase (GS) in nectar, nectaries, and leaves were analyzed in 30 Pitcairnia species. These data were compared with various floral traits, the pollinator type, and the phylogenetic relationships of the species to find possible causes for the high amino acid concentrations in the nectar of some species. The highest concentrations of amino acids (especially alanine) in nectar were found in species with reddish flowers. Furthermore, the concentration of amino acids in nectar and nectaries is determined through analyzing flower color/pollination type rather than phylogenetic relations. This study provides new insights into the origin of amino acids in nectar. The presence of almost all amino acids in nectar is mainly due to their transport in the phloem to the nectaries, with the exception of alanine, which is partially produced in nectaries. In addition, active regulatory mechanisms are required in nectaries that retain most of the amino acids and allow the selective secretion of specific amino acids, such as alanine.
RESUMO
Floral nectar contains mainly sugars as well as smaller amounts of amino acids and further compounds. The nectar composition varies between different plant species and it is related to the pollination type of the plant. In addition to this, other factors can influence the composition. Nectar is produced in and secreted from nectaries. A few models exist to explain the origin of nectar for dicotyl plant species, a complete elucidation of the processes, however, has not yet been achieved. This is particularly true for monocots or plant species with CAM photosynthesis. To get closer to such an elucidation, nectar, nectaries, and leaves of 36 bromeliad species were analyzed for sugars, starch, amino acids, and inorganic ions. The species studied include different photosynthesis types (CAM/C3), different pollination types (trochilophilous/chiropterophilous), or different live forms. The main sugars in nectar and nectaries were glucose, fructose, and sucrose, the total sugar concentration was about twofold higher in nectar than in nectaries, which suggests that sugars are actively transported from the nectaries into the nectar. The composition of amino acids in nectar is already determined in the nectaries, but the concentration is much lower in nectar than in nectaries, which suggests selective retention of amino acids during nectar secretion. The same applies to inorganic ions. Statistical analyses showed that the photosynthesis type and the pollination type can explain more data variation in nectar than in nectaries and leaves. Furthermore, the pollinator type has a stronger influence on the nectar or nectary composition than the photosynthesis type. Trochilophilous C3 plants showed significant correlations between the nitrate concentration in leaves and the amino acid concentration in nectaries and nectar. It can be assumed that the more nitrate is taken up, the more amino acids are synthesized in leaves and transported to the nectaries and nectar. However, chiropterophilous C3 plants show no such correlation, which means that the secretion of amino acids into the nectar is regulated by further factors. The results help understand the physiological properties that influence nectaries and nectar as well as the manner of metabolite and ion secretion from nectaries to nectar.
RESUMO
Floral nectar is the most important reward for pollinators and an integral component of the pollination syndrome. Nectar research has mainly focused on sugars or amino acids, whereas more comprehensive studies on the nectar composition of closely related plant species with different pollination types are rather limited. Nectar composition as well as concentrations of sugars, amino acids, inorganic ions, and organic acids were analyzed for 147 species of Bromeliaceae. This plant family shows a high diversity in terms of floral morphology, flowering time, and predominant pollination types (trochilophilous, trochilophilous/entomophilous, psychophilous, sphingophilous, chiropterophilous). Based on the analyses, we examined the relationship between nectar traits and pollination type in this family. Nectar of all analyzed species contained high amounts of sugars with different proportions of glucose, fructose, and sucrose. The total concentrations of amino acids, inorganic cations, and anions, or organic acids were much lower. The analyses revealed that the sugar composition, the concentrations of inorganic cations and anions as well as the concentration of malate in nectar of bat-pollinated species differed significantly from nectar of species with other pollination types. Flowers of bat-pollinated species contained a higher volume of nectar, which results in a total of about 25-fold higher amounts of sugar in bat-pollinated species than in insect-pollinated species. This difference was even higher for amino acids, inorganic anions and cations, and organic acids (between 50 and 100-fold). In general, bat-pollinated plant species invest large amounts of organic and inorganic compounds for their pollinators. Furthermore, statistical analyses reveal that the characteristics of nectar in Bromeliaceae are more strongly determined by the pollinator type rather than by taxonomic groups or phylogenetic relations. However, a considerable part of the variance cannot be explained by either of the variables, which means that additional factors must be responsible for the differences in the nectar composition.