Messing with Merton: The intersection between open science practices and Mertonian values.

Although adherence to Mertonian values of science (i.e., communism, universalism, organized skepticism, disinterestedness) is desired and promoted in academia, such adherence can cause friction with the normative structures and practices of Open Science. Mertonian values and Open Science practices aim to improve the conduct and communication of research and are promoted by institutional actors. However, Mertonian values remain mostly idealistic and contextualized in local and disciplinary cultures and Open Science practices rely heavily on third-party resources and technology that are not equally accessible to all parties. Furthermore, although still popular, Mertonian values were developed in a different institutional and political context. In this article, we argue that new normative structures for science need to look beyond nostalgia and consider aspirations and outcomes of Open Science practices. To contribute to such a vision, we explore the intersection of several Open Science practices with Mertonian values to flesh out challenges involved in upholding these values. We demonstrate that this intersection becomes complicated when the interests of numerous groups collide and contrast. Acknowledging and exploring such tensions informs our understanding of researchers' behavior and supports efforts that seek to improve researchers' interactions with other normative structures such as research ethics and integrity frameworks.

Mesoangioblasts at 20: From the embryonic aorta to the patient bed.

In 2002 we published an article describing a population of vessel-associated progenitors that we termed mesoangioblasts (MABs). During the past decade evidence had accumulated that during muscle development and regeneration things may be more complex than a simple sequence of binary choices (e.g., dorsal vs. ventral somite). LacZ expressing fibroblasts could fuse with unlabelled myoblasts but not among themselves or with other cell types. Bone marrow derived, circulating progenitors were able to participate in muscle regeneration, though in very small percentage. Searching for the embryonic origin of these progenitors, we identified them as originating at least in part from the embryonic aorta and, at later stages, from the microvasculature of skeletal muscle. While continuing to investigate origin and fate of MABs, the fact that they could be expanded in vitro (also from human muscle) and cross the vessel wall, suggested a protocol for the cell therapy of muscular dystrophies. We tested this protocol in mice and dogs before proceeding to the first clinical trial on Duchenne Muscular Dystrophy patients that showed safety but minimal efficacy. In the last years, we have worked to overcome the problem of low engraftment and tried to understand their role as auxiliary myogenic progenitors during development and regeneration.

Covid-19 and the need for more history and philosophy of RNA.

RNA is central to the COVID-19 pandemic-it shapes how the SARS Coronavirus 2 (SARS-CoV-2) behaves, and how researchers investigate and fight it. However, RNA has received relatively little attention in the history and philosophy of the life sciences. By analysing RNA biology in more detail, philosophers and historians of science could gain new and powerful tools to assess the current pandemic, and the biological sciences more generally.

Co/Ni-polyoxotungstate photocatalysts as precursor materials for electrocatalytic water oxidation.

An open-core cobalt polyoxometalate (POM) [(A-α-SiW9O34)Co4(OH)3(CH3COO)3]8-Co(1) and its isostructural Co/Ni-analogue [(A-α-SiW9O34)Co1.5Ni2.5(OH)3(CH3COO)3]8-CoNi(2) were synthesized and investigated for their photocatalytic and electrocatalytic performance. Co(1) shows high photocatalytic O2 yields, which are competitive with leading POM water oxidation catalysts (WOCs). Furthermore, Co(1) and CoNi(2) were employed as well-defined precursors for heterogeneous WOCs. Annealing at various temperatures afforded amorphous and crystalline CoWO4- and Co1.5Ni2.5WO4-related nanoparticles. CoWO4-related particles formed at 300 °C showed substantial electrocatalytic improvements and were superior to reference materials obtained from co-precipitation/annealing routes. Interestingly, no synergistic interactions between cobalt and nickel centers were observed for the mixed-metal POM precursor and the resulting tungstate catalysts. This stands in sharp contrast to a wide range of studies on various heterogeneous catalyst types which were notably improved through Co/Ni substitution. The results clearly demonstrate that readily accessible POMs are promising precursors for the convenient and low-temperature synthesis of amorphous heterogeneous water oxidation catalysts with enhanced performance compared to conventional approaches. This paves the way to tailoring polyoxometalates as molecular precursors with tuneable transition metal cores for high performance heterogeneous electrocatalysts. Our results furthermore illustrate the key influence of the synthetic history on the performance of oxide catalysts and highlight the dependence of synergistic metal interactions on the structural environment.

Pathways towards true catalysts: computational modelling and structural transformations of Zn-polyoxotungstates.

Current catalysis undergoes a paradigm shift from molecular and heterogeneous realms towards new dynamic catalyst concepts. This calls for innovative strategies to understand the essential catalytic motifs and true catalysts emerging from oxidative transformation processes. Polyoxometalate (POM) clusters offer an inexhaustible reservoir for new noble metal-free catalysts and excellent model systems whose structure-activity relationships and mechanisms remain to be explored. Here, we first introduce a new {ZnnNa6-n(B-α-SbW9O33)2} (n = 3-6) catalyst family with remarkable tuning options of the Zn-based core structure and high activity in H2O2-assisted catalytic alcohol oxidation as a representative reaction. Next, high level solution-based computational modelling of the intermediates and transition states was carried out for [Zn6Cl6(SbW9O33)2]12- as a representative well-defined case. The results indicate a radical-based oxidation process with the involvement of tungsten and adjacent zinc metal centers. The {ZnnNa6-n(B-α-SbW9O33)2} series indeed efficiently catalyses alcohol oxidation via peroxotungstate intermediates, in agreement with strong spectroscopic support and other experimental evidence for the radical mechanism. Finally, the high performance of [Zn6Cl6(SbW9O33)2]12- was traced back to its transformation into a highly active and robust disordered Zn/W-POM catalyst. The atomic short-range structure of this resting pre-catalyst was elucidated by RMC modelling of the experimental W-L3 and Zn-K edge EXAFS spectra and supported with further analytical methods. We demonstrate that computational identification of the reactive sites combined with the analytical tracking of their dynamic transformations provides essential input to expedite cluster-based molecular catalyst design.

Characterizing scientific failure: Putting the replication crisis in context.

A better understanding of the nature and causes of failure in research could inform policies to improve the reproducibility of biomedical research.

Quantum transport through MoS2 constrictions defined by photodoping.

We present a device scheme to explore mesoscopic transport through molybdenum disulfide (MoS2) constrictions using photodoping. The devices are based on van-der-Waals heterostructures where few-layer MoS2 flakes are partially encapsulated by hexagonal boron nitride (hBN) and covered by a few-layer graphene flake to fabricate electrical contacts. Since the as-fabricated devices are insulating at low temperatures, we use photo-induced remote doping in the hBN substrate to create free charge carriers in the MoS2 layer. On top of the device, we place additional metal structures, which define the shape of the constriction and act as shadow masks during photodoping of the underlying MoS2/hBN heterostructure. Low temperature two- and four-terminal transport measurements show evidence of quantum confinement effects.

Trust in Science: CRISPR-Cas9 and the Ban on Human Germline Editing.

In 2015 scientists called for a partial ban on genome editing in human germline cells. This call was a response to the rapid development of the CRISPR-Cas9 system, a molecular tool that allows researchers to modify genomic DNA in living organisms with high precision and ease of use. Importantly, the ban was meant to be a trust-building exercise that promises a 'prudent' way forward. The goal of this paper is to analyse whether the ban can deliver on this promise. To do so the focus will be put on the precedent on which the current ban is modelled, namely the Asilomar ban on recombinant DNA technology. The analysis of this case will show (a) that the Asilomar ban was successful because of a specific two-step containment strategy it employed and (b) that this two-step approach is also key to making the current ban work. It will be argued, however, that the Asilomar strategy cannot be transferred to human genome editing and that the current ban therefore fails to deliver on its promise. The paper will close with a reflection on the reasons for this failure and on what can be learned from it about the regulation of novel molecular tools.

High Quality Factor Graphene-Based Two-Dimensional Heterostructure Mechanical Resonator.

Ultralight mechanical resonators based on low-dimensional materials are well suited as exceptional transducers of minuscule forces or mass changes. However, the low dimensionality also provides a challenge to minimize resistive losses and heating. Here, we report on a novel approach that aims to combine different two-dimensional (2D) materials to tackle this challenge. We fabricated a heterostructure mechanical resonator consisting of few layers of niobium diselenide (NbSe2) encapsulated by two graphene sheets. The hybrid membrane shows high quality factors up to 245,000 at low temperatures, comparable to the best few-layer graphene mechanical resonators. In contrast to few-layer graphene resonators, the device shows reduced electrical losses attributed to the lower resistivity of the NbSe2 layer. The peculiar low-temperature dependence of the intrinsic quality factor points to dissipation over two-level systems which in turn relax over the electronic system. Our high sensitivity readout is enabled by coupling the membrane to a superconducting cavity which allows for the integration of the hybrid mechanical resonator as a sensitive and low loss transducer in future quantum circuits.

Frontiers of water oxidation: the quest for true catalysts.

Development of efficient and economic water oxidation catalysts (WOCs) remains a crucial bottleneck on the way to artificial photosynthesis applications. Over the past few decades, WOC research has turned into a fascinating interdisciplinary field that ranges from bio-inspired molecular design over nanomaterials and thin films to solid materials tuning. Under the umbrella of WOC optimization, advanced in situ/operando analytical techniques are being developed as increasingly powerful tools to elucidate the controversial discussions about the molecular or nanoscale nature of many WOCs. More and more of these approaches also enable the monitoring of possible key intermediates as an essential prerequisite for proposing catalytic mechanisms. This review is organized in three main parts: first, recent highlights outline frontiers in WOC development, such as the benefits of connecting molecular WOCs with solids along with the introduction of molecular concepts into heterogeneous WOC research. Next, a brief overview of emerging in situ/operando approaches demonstrates new options for monitoring WOC transformations. Finally, selected monitoring studies over the entire WOC dimensionality spectrum illustrate interesting cases of catalytic border crossings as new input for WOC construction.

Energy-dependent path of dissipation in nanomechanical resonators.

Energy decay plays a central role in a wide range of phenomena, such as optical emission, nuclear fission, and dissipation in quantum systems. Energy decay is usually described as a system leaking energy irreversibly into an environmental bath. Here, we report on energy decay measurements in nanomechanical systems based on multilayer graphene that cannot be explained by the paradigm of a system directly coupled to a bath. As the energy of a vibrational mode freely decays, the rate of energy decay changes abruptly to a lower value. This finding can be explained by a model where the measured mode hybridizes with other modes of the resonator at high energy. Below a threshold energy, modes are decoupled, resulting in comparatively low decay rates and giant quality factors exceeding 1 million. Our work opens up new possibilities to manipulate vibrational states, engineer hybrid states with mechanical modes at completely different frequencies, and to study the collective motion of this highly tunable system.

Force sensitivity of multilayer graphene optomechanical devices.

Mechanical resonators based on low-dimensional materials are promising for force and mass sensing experiments. The force sensitivity in these ultra-light resonators is often limited by the imprecision in the measurement of the vibrations, the fluctuations of the mechanical resonant frequency and the heating induced by the measurement. Here, we strongly couple multilayer graphene resonators to superconducting cavities in order to achieve a displacement sensitivity of 1.3 fm Hz(-1/2). This coupling also allows us to damp the resonator to an average phonon occupation of 7.2. Our best force sensitivity, 390 zN Hz(-1/2) with a bandwidth of 200 Hz, is achieved by balancing measurement imprecision, optomechanical damping, and measurement-induced heating. Our results hold promise for studying the quantum capacitance of graphene, its magnetization, and the electron and nuclear spins of molecules adsorbed on its surface.

Reactive Pulmonary Capillary Hemangiomatosis and Pulmonary Veno-Occlusive Disease in a Patient with Repaired Scimitar Syndrome.

Pulmonary capillary hemangiomatosis (PCH) is a rare histological substrate within the spectrum of pulmonary arterial hypertension that possibly represents an unusual manifestation of pulmonary veno-occlusive disease (PVOD). One of the histological hallmarks of PCH is the proliferation of pulmonary capillaries in the alveolar septa that infiltrate adjacent structures such as bronchioles, vessels, and visceral pleura. The hyperplastic process involving the smallest vessels of the pulmonary vascular bed might reflect uncontrolled angiogenesis, but whether this vascular proliferation is idiopathic or, conversely, a reactive process remains to be elucidated. Here we discuss the pathogenesis of PCH exemplified by the first reported case of a young patient with repaired scimitar syndrome that developed unilateral PCH.

Viruses as living processes.

The view that life is composed of distinct entities with well-defined boundaries has been undermined in recent years by the realisation of the near omnipresence of symbiosis. What had seemed to be intrinsically stable entities have turned out to be systems stabilised only by the interactions between a complex set of underlying processes (Dupré, 2012). This has not only presented severe problems for our traditional understanding of biological individuality but has also led some to claim that we need to switch to a process ontology to be able adequately to understand biological systems. A large group of biological entities, however, has been excluded from these discussions, namely viruses. Viruses are usually portrayed as stable and distinct individuals that do not fit the more integrated and collaborative picture of nature implied by symbiosis. In this paper we will contest this view. We will first discuss recent findings in virology that show that viruses can be 'nice' and collaborate with their hosts, meaning that they form part of integrated biological systems and processes. We further offer various reasons why viruses should be seen as processes rather than things, or substances. Based on these two claims we will argue that, far from serving as a counterexample to it, viruses actually enable a deeper understanding of the fundamentally interconnected and collaborative nature of nature. We conclude with some reflections on the debate as to whether viruses should be seen as living, and argue that there are good reasons for an affirmative answer to this question.

Electromechanical control of nitrogen-vacancy defect emission using graphene NEMS.

Despite recent progress in nano-optomechanics, active control of optical fields at the nanoscale has not been achieved with an on-chip nano-electromechanical system (NEMS) thus far. Here we present a new type of hybrid system, consisting of an on-chip graphene NEMS suspended a few tens of nanometres above nitrogen-vacancy centres (NVCs), which are stable single-photon emitters embedded in nanodiamonds. Electromechanical control of the photons emitted by the NVC is provided by electrostatic tuning of the graphene NEMS position, which is transduced to a modulation of NVC emission intensity. The optomechanical coupling between the graphene displacement and the NVC emission is based on near-field dipole-dipole interaction. This class of optomechanical coupling increases strongly for smaller distances, making it suitable for nanoscale devices. These achievements hold promise for selective control of emitter arrays on-chip, optical spectroscopy of individual nano-objects, integrated optomechanical information processing and open new avenues towards quantum optomechanics.

Predictors of Outcome in Pediatric Osteomyelitis: Five Years Experience in a Single Tertiary Center.

BACKGROUND: Acute haematogenous osteomyelitis is a bacterial infection of bone, which occurs most frequently in children. Outcomes are excellent for the majority of children, but a minority develop complicated osteomyelitis. Predicting which children will develop complicated osteomyelitis remains a challenge, particularly in developed countries where most patients are discharged home after a relatively short period in hospital. METHODS: We conducted a 5-year retrospective case note review of all children aged 3 months to 16 years admitted with a diagnosis of acute haematogenous osteomyelitis. We compared standardized clinical and laboratory parameters in those who developed simple and complicated osteomyelitis. RESULTS: Of the 299 children who met inclusion, 241 (80.6%) had simple and 58 (19.4%) had complicated osteomyelitis. The major predictors of complicated disease were older age, a temperature greater than 38.5°C and a higher C-reactive protein at admission. CONCLUSIONS: A risk prediction model, utilizing information available shortly after hospitalization, allows early identification of children at greatest risk of developing complicated osteomyelitis.

Nanotube mechanical resonators with quality factors of up to 5 million.

Carbon nanotube mechanical resonators have attracted considerable interest because of their small mass, the high quality of their surfaces, and the pristine electronic states they host. However, their small dimensions result in fragile vibrational states that are difficult to measure. Here, we observe quality factors Q as high as 5 × 10(6) in ultra-clean nanotube resonators at a cryostat temperature of 30 mK, where we define Q as the ratio of the resonant frequency over the linewidth. Measuring such high quality factors requires the use of an ultra-low-noise method to rapidly detect minuscule vibrations, as well as careful reduction of the noise of the electrostatic environment. We observe that the measured quality factors fluctuate because of fluctuations of the resonant frequency. We measure record-high quality factors, which are comparable to the highest Q values reported in mechanical resonators of much larger size, a remarkable result considering that reducing the size of resonators is usually concomitant with decreasing quality factors. The combination of ultra-low mass and very large Q offers new opportunities for ultra-sensitive detection schemes and quantum optomechanical experiments.