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4.
Transplant Proc ; 46(6): 1867-71, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25131056

RESUMO

BACKGROUND: Liver regeneration has great importance for transplantation, especially in children; however, it has not been studied sufficiently in development animals. Ischemia-reperfusion injury is a problem, and strategies such as ischemic preconditioning and postconditioning are not well defined regarding regeneration. OBJECTIVE: This study sought to evaluate liver regeneration with modulation by ischemic preconditioning and postconditioning in prepubertal rats subjected to total ischemia and reperfusion. METHODS: Thirty-five 5-week-old female Wistar rats were divided into groups of 7 animals each: control group (SHAM), 70% hepatectomy (HEP), total ischemia 30 minutes before hepatectomy (IR), ischemic preconditioning 10/10 minutes before ischemia (PRE), and two 30/30-second ischemic postconditioning cycles after ischemia and hepatectomy (POS). All animals were subjected to 24-hour reperfusion. Aspartate aminotransferase and alanine aminotransferase activity were measured to evaluate liver damage, and histological analysis, proliferating cell nuclear antigen (PCNA) and regenerated mass liver were used to evaluate liver regeneration. Statistical analyses were performed using ANOVA and Kruskal-Wallis test. RESULTS: Alanine aminotransferase and aspartate aminotransferase levels were significantly lower in conditioned groups than in the IR group. Regarding mitotic index, IR > control group and HEP (P < .05), PRE and POS were not significantly different from IR, and POS > HEP (P < .05). PCNA analysis showed that IR > HEP (P < .01), PRE < IR (P < .01), and no significant differences were observed between POS and IR groups. No significant differences in regenerated mass liver were observed between conditioned groups and HEP. CONCLUSIONS: Ischemic postconditioning prevented ischemic injury, promoted greater liver regeneration, and should be further investigated as an alternative better than ischemic preconditioning.


Assuntos
Pós-Condicionamento Isquêmico/métodos , Precondicionamento Isquêmico/métodos , Hepatopatias/cirurgia , Regeneração Hepática , Traumatismo por Reperfusão/prevenção & controle , Animais , Modelos Animais de Doenças , Feminino , Hepatectomia , Hepatopatias/patologia , Ratos , Ratos Wistar
5.
Br J Dermatol ; 167(4): 837-46, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22950597

RESUMO

BACKGROUND: American tegumentary leishmaniasis (ATL) and sporotrichosis exhibit similar histopathology and low frequencies of microorganism detection. OBJECTIVES: This study seeks to identify microscopic alterations that can distinguish between these diseases. METHODS: Haematoxylin and eosin stained slides of 171 ATL and 97 sporotrichosis samples from active cutaneous lesions were examined for histopathological alterations. The lesions were diagnosed by isolating the agent (which was not visible) in culture. An intuitive diagnosis was assigned to each slide. The strength of the association between the histopathological findings and the diagnosis was estimated by an odds ratio, and each finding was graded according to a regression model. A score was assigned to each sample based on the histopathological findings. A study of the interobserver reliability was performed by calculating kappa coefficients of the histopathological findings and intuitive diagnoses. RESULTS: The markers 'macrophage concentration', 'tuberculoid granuloma' and 'extracellular matrix degeneration' were associated with ATL. 'Suppurative granuloma', 'stellate granuloma', 'different types of giant cells', 'granulomas in granulation tissue' and 'abscess outside the granuloma' were associated with a diagnosis of sporotrichosis. 'Macrophage concentration' and 'suppurative granuloma' had the highest (substantial and almost perfect, respectively) reliability. The regression model score indicated 92.0% accuracy. The intuitive diagnosis had 82.5% diagnostic accuracy and substantial reliability. CONCLUSIONS: Taking into account the clinical and epidemiological context, some histopathological alterations might be useful for the differential diagnosis between ATL and sporotrichosis cutaneous lesions in cases in which the aetiological agent is not visible.


Assuntos
Dermatomicoses/diagnóstico , Leishmaniose Cutânea/diagnóstico , Dermatopatias Parasitárias/diagnóstico , Esporotricose/diagnóstico , Brasil , Estudos Transversais , Diagnóstico Diferencial , Granuloma/patologia , Humanos , Modelos Logísticos , Macrófagos/patologia , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Coloração e Rotulagem
7.
J Eur Acad Dermatol Venereol ; 23(11): 1273-6, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19573026

RESUMO

BACKGROUND: There are a few studies on the treatment of sporotrichosis. The standard drug used is itraconazole. However, the use of itraconazole is limited by its interaction with other drugs. OBJECTIVE: To evaluate the effectiveness and safety of 250 mg terbinafine for the treatment of cutaneous sporotrichosis in patients in whom itraconazole use is not possible. METHODS: We performed a descriptive study of cutaneous sporotrichosis cases treated with 250 mg terbinafine for which itraconazole was contraindicated or resulted in severe or moderate pharmacological interactions. Sporotrichosis was diagnosed based on the isolation of S. schenckii. RESULTS: Fifty patients seen between July 2005 and September 2007 were included. Forty-five (92%) patients reported contact with a sick cat and 47 (94%) presented comorbidities (high blood pressure: 64.0%; diabetes mellitus: 30.0%; dyslipidemia: 16.7%; depression: 10.0%; migraine: 2.1%; Parkinsonís disease: 2.1%; peptic ulcer disease: 2.1%; heart failure: 2.1%, and arrhythmia: 2.1%). All patients used some medication interacting with itraconazole (psycholeptics: 36.0%; antidiabetic agents: 28.0%; hypolipemiant agents: 18.0%; calcium-channel blockers: 16.0%; anticonvulsants: 8.0%; cardiotropic drugs: 6.3%; antacids: 6.3%, and antiparkinsonian agent: 2.1%). Most patients (96%) were cured within a mean period of 14 weeks. The drug was discontinued due to a skin rash in one patient. There were no cases of recurrence of the mycosis within a mean follow-up period of 37 weeks. CONCLUSIONS: This study suggests that 250 mg/day terbinafine is an effective and well-tolerated alternative to drug therapy of cutaneous sporotrichosis in a population in which itraconazole use is not possible.


Assuntos
Antifúngicos/uso terapêutico , Naftalenos/uso terapêutico , Esporotricose/tratamento farmacológico , Humanos , Terbinafina
10.
Med Mycol ; 42(3): 229-38, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15283237

RESUMO

A high biodiversity of Cryptococcus neoformans isolates is known to exist in some Brazilian urban areas, raising the possibility that patients may encounter multiple inoculum sources in their daily life. C. neoformans isolates from two groups of AIDS patients with cryptococcosis from Rio de Janeiro were studied by polymerase chain reaction (PCR) fingerprinting and randomly amplified polymorphic DNA (RAPD) analysis. The first group contained 60 serial isolates obtained from 19 patients over periods ranging from 18 to 461 days; the intent was to determine whether the original strain persisted or whether reinfection with a new strain occurred. The second group was made up of 22 isolates from 11 patients, and consisted of a pair of isolates collected from blood and cerebrospinal fluid from each patient either before or shortly after treatment was initiated. The aim was to determine if the patient was infected by different strains simultaneously. All isolates were subtyped by PCR fingerprinting, using minisatellite (M13), and microsatellite [(GACA)4 and (GTG)5] specific primers, and RAPD analysis employing the combined primers 5SOR and CN1. The majority of isolates were C. neoformans var. grubii, specifically, molecular types VNI or VNII, but numerous distinguishable subtypes were found. Only three isolates were C. n. var. gattii (molecular types VGI or VGII). Except in two cases, all isolates obtained from the same patient showed identical PCR profiles independent of time of isolation or body site. Almost all patients, however, carried unique genotypes not found in any other patient. Our results confirm that persistent cryptococcal infection is caused by relapse rather than reinfection, but they also show that in exceptional cases, patients may be infected with more than one C. neoformans strain.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Síndrome da Imunodeficiência Adquirida/complicações , Criptococose/microbiologia , Cryptococcus neoformans/genética , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Adulto , Sangue/microbiologia , Brasil/epidemiologia , Líquido Cefalorraquidiano/microbiologia , Análise por Conglomerados , Criptococose/epidemiologia , Cryptococcus neoformans/isolamento & purificação , Impressões Digitais de DNA , DNA Fúngico/análise , DNA Fúngico/isolamento & purificação , Feminino , Fungemia/microbiologia , Genótipo , Humanos , Masculino , Repetições de Microssatélites/genética , Pessoa de Meia-Idade , Repetições Minissatélites/genética , Epidemiologia Molecular , Técnicas de Tipagem Micológica , Técnica de Amplificação ao Acaso de DNA Polimórfico , Recidiva
11.
Mycoses ; 47(5-6): 238-41, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15189192

RESUMO

A 51-year-old white male, native of Rio de Janeiro, Brazil, with advanced AIDS and in chronic use of imidazoles for oral candidosis, presented erythematous, desquamative, pruriginous plaques of 1 month evolution on the trunk, inguinal/crural region, and lower limbs. The diagnosis of dermatophytosis was based on the isolation of Microsporum gypseum from scales scraped from the skin lesions. The lesions regressed after 30 days treatment with itraconazole, 100 mg day(-1). After interruption of this antifungal, the mycosis recurred 2 months later, along with a toe onychomycosis also caused by M. gypseum. Attempted reintroduction of itraconazole at higher dose was unsuccessful. Patient responded well to treatment with terbinafine 250 mg day(-1) for 45 days. However, the medication had to be interrupted as a result of the emergence of a disseminated erythematous/papulous rash. Topical treatment with amorolfine cream was then performed, with a good clinical and mycological response.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Síndrome da Imunodeficiência Adquirida/complicações , Antifúngicos/uso terapêutico , Dermatomicoses/tratamento farmacológico , Microsporum , Morfolinas/uso terapêutico , Naftalenos/uso terapêutico , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Adulto , Dermatomicoses/patologia , Humanos , Masculino , Terbinafina , Resultado do Tratamento
12.
Braz. j. med. biol. res ; 37(5): 745-753, May 2004. ilus, tab
Artigo em Inglês | LILACS | ID: lil-357548

RESUMO

Dysregulation of the skin immune system (SIS) could explain the high prevalence of skin disorders in HIV+ individuals. The present study was carried out to determine whether alterations in the cell population of SIS and epidermal immunoactivation occur in the normal skin of HIV+ individuals. Forty-five biopsies were taken from the normal upper arm skin of 45 HIV+ patients and of 15 healthy controls. HIV+ individuals were divided into three categories according to their CD4 cell blood count (<200, 200-499 and 500/æl). Hematoxylin-eosin was used to stain tissue sections for morphological analysis and immunohistochemistry was used for the evaluation of the frequency of macrophages, Langerhans cells, and CD lymphocyte subsets. In addition, semiquantitative analysis of LFA-1, ICAM-1 and HLA-DR was determined in epidermal cells. Macrophages, Langerhans cells, and CD lymphocyte subsets did not differ significantly between any of the patient categories and the control group. When all HIV+ individuals were compared as a group to the control group, a significant increase in dermal CD8+ T lymphocytes (P < 0.01) and lower CD4-CD8 ratios (P < 0.01) were observed in the HIV+ individuals. Epidermal ICAM-1 and HLA-DR expression was negative in both HIV+ and normal skin biopsies. No evidence of a depletion of the SIS population or of epidermal immunoactivation in normal skin from HIV+ individuals was demonstrable, suggesting that alterations in the central immune system are not necessarily reflected in the SIS of HIV-infected patients.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Linfócitos T CD8-Positivos , Infecções por HIV , Células de Langerhans , Pele , Biópsia , Estudos de Casos e Controles , Imuno-Histoquímica
13.
Braz J Med Biol Res ; 37(5): 745-53, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15107938

RESUMO

Dysregulation of the skin immune system (SIS) could explain the high prevalence of skin disorders in HIV+ individuals. The present study was carried out to determine whether alterations in the cell population of SIS and epidermal immunoactivation occur in the normal skin of HIV+ individuals. Forty-five biopsies were taken from the normal upper arm skin of 45 HIV+ patients and of 15 healthy controls. HIV+ individuals were divided into three categories according to their CD4 cell blood count (<200, 200-499 and > or = 500/microl). Hematoxylin-eosin was used to stain tissue sections for morphological analysis and immunohistochemistry was used for the evaluation of the frequency of macrophages, Langerhans cells, and CD lymphocyte subsets. In addition, semiquantitative analysis of LFA-1, ICAM-1 and HLA-DR was determined in epidermal cells. Macrophages, Langerhans cells, and CD lymphocyte subsets did not differ significantly between any of the patient categories and the control group. When all HIV+ individuals were compared as a group to the control group, a significant increase in dermal CD8+ T lymphocytes (P < 0.01) and lower CD4-CD8 ratios (P < 0.01) were observed in the HIV+ individuals. Epidermal ICAM-1 and HLA-DR expression was negative in both HIV+ and normal skin biopsies. No evidence of a depletion of the SIS population or of epidermal immunoactivation in normal skin from HIV+ individuals was demonstrable, suggesting that alterations in the central immune system are not necessarily reflected in the SIS of HIV-infected patients.


Assuntos
Linfócitos T CD8-Positivos/imunologia , Infecções por HIV/patologia , Células de Langerhans/patologia , Pele/patologia , Adulto , Biópsia , Relação CD4-CD8 , Estudos de Casos e Controles , Feminino , Infecções por HIV/imunologia , Humanos , Imuno-Histoquímica , Células de Langerhans/imunologia , Masculino , Pessoa de Meia-Idade , Pele/imunologia
14.
Vet Rec ; 152(6): 172-5, 2003 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-12622288

RESUMO

Ten cats with sporotrichosis were examined clinically and pathologically. They were in very poor general condition, and had widespread ulcerated cutaneous lesions and respiratory signs. Gross internal abnormalities were found only in the lungs and lymph nodes. Histologically, an inflammatory infiltrate and yeast-like structures were observed in the skin, lungs, liver and lymph nodes. The spleen was congested and contained fungal elements. No microscopical changes were observed in the pancreas, kidneys and heart. Sporothix schenckii was isolated from all the skin samples and nasal swabs obtained in vivo, and from all the samples of lung, liver, spleen, lymph nodes, heart and kidney taken postmortem.


Assuntos
Doenças do Gato/microbiologia , Doenças do Gato/patologia , Esporotricose/patologia , Esporotricose/veterinária , Animais , Brasil , Doenças do Gato/diagnóstico por imagem , Doenças do Gato/tratamento farmacológico , Gatos , Feminino , Itraconazol/uso terapêutico , Pulmão/diagnóstico por imagem , Pulmão/microbiologia , Pulmão/patologia , Masculino , Radiografia , Esporotricose/diagnóstico por imagem , Esporotricose/tratamento farmacológico
15.
Braz. j. infect. dis ; 6(6): 272-275, Dec. 2002. tab
Artigo em Inglês | LILACS | ID: lil-348944

RESUMO

A prospective study was conducted on 79 advanced immunosuppressed AIDS patients from 1997 to 1999, during which nine cases of tuberculosis (TB) were diagnosed. The main clinical and laboratory characteristics and the response to TB treatment were reviewed. The clinical manifestations of TB were: pulmonary (six cases), extrapulmonary (two cases) and disseminated (one case). These patients were being treated with highly active antiretroviral treatment (HAART) and were not responding. In three cases an optional regimen without rifampicin (RMP) was indicated to maintain HAART during TB treatment. A clinical response to TB treatment (disappearance of fever) was observed in 6/9 patients during a mean of 73 days (SD = 96). The three unresponsive patients were those treated without RMP. A switch to TB regimens containing RMP was proposed and successful. In our study, though it was limited by a small sample size, the response to TB regimens without rifampin was poor in immunossupressed patients failing HAART.


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Antibióticos Antituberculose/uso terapêutico , Rifampina/uso terapêutico , Tuberculose Pulmonar/tratamento farmacológico , Infecções Oportunistas Relacionadas com a AIDS/complicações , Terapia Antirretroviral de Alta Atividade , Quimioterapia Combinada , Hospedeiro Imunocomprometido , Estudos Prospectivos , Resultado do Tratamento , Tuberculose Pulmonar/complicações
16.
Braz J Infect Dis ; 6(6): 272-5, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12585969

RESUMO

A prospective study was conducted on 79 advanced immunosuppressed AIDS patients from 1997 to 1999, during which nine cases of tuberculosis (TB) were diagnosed. The main clinical and laboratory characteristics and the response to TB treatment were reviewed. The clinical manifestations of TB were: pulmonary (six cases), extrapulmonary (two cases) and disseminated (one case). These patients were being treated with highly active antiretroviral treatment (HAART) and were not responding. In three cases an optional regimen without rifampicin (RMP) was indicated to maintain HAART during TB treatment. A clinical response to TB treatment (disappearance of fever) was observed in 6/9 patients during a mean of 73 days (SD = 96). The three unresponsive patients were those treated without RMP. A switch to TB regimens containing RMP was proposed and successful. In our study, though it was limited by a small sample size, the response to TB regimens without rifampin was poor in immunosupressed patients failing HAART.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Antibióticos Antituberculose/uso terapêutico , Rifampina/uso terapêutico , Tuberculose Pulmonar/tratamento farmacológico , Infecções Oportunistas Relacionadas com a AIDS/complicações , Adulto , Terapia Antirretroviral de Alta Atividade , Quimioterapia Combinada , Feminino , Humanos , Hospedeiro Imunocomprometido , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Resultado do Tratamento , Tuberculose Pulmonar/complicações
17.
Rev Inst Med Trop Sao Paulo ; 43(5): 283-6, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11696852

RESUMO

We present the case of a 15-year-old patient infected with HTLV-1 who developed a cutaneous T-cell lymphoma, confirmed by histopathological and immunohistochemical examination, as well as clinically and hematologically confirmed leukemia. The patient died 3 months after initial presentation of the disease. The rarity of the disease in this age group justifies the present report.


Assuntos
Leucemia-Linfoma de Células T do Adulto/diagnóstico , Neoplasias Cutâneas/diagnóstico , Adolescente , Brasil , Evolução Fatal , Feminino , Humanos , Leucemia-Linfoma de Células T do Adulto/patologia , Neoplasias Cutâneas/patologia
18.
Infection ; 29(5): 257-61, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11688902

RESUMO

BACKGROUND: Cytokines may alter metabolic pathways and contribute to malnutrition among human immunodefiency virus (HIV)-positive individuals. PATIENTS AND METHODS: Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), soluble IL-2 receptors (sIL-2R), beta2-microglobulin serum levels and plasma viral load of 45 HIV-positive patients were determined and correlated to nutritional status impairment. Patients were grouped by CD4 counts into categories I (< 200/microl), II (200-499/microl), III (> or = 500/microl). There were 15 healthy controls. A nutritional grading system, based on anthropometric and laboratory data, was devised. Scores ranged from 0 to 5 (eutrophic to malnutrition). RESULTS: AIDS patients' cytokines and immune marker levels were significantly higher than those of the controls, but not always higher than those of other categories. AIDS patients had higher nutritional deficit grades than category III (p < 0.05) or the controls (p < 0.02) which, except for viral load, correlated with the parameters studied. CONCLUSION: Nutritional status impairments in HIV-positive individuals were associated with immune activation but not with viral load.


Assuntos
Infecções por HIV/complicações , Interleucina-6/sangue , Distúrbios Nutricionais/fisiopatologia , Estado Nutricional , Fator de Necrose Tumoral alfa/análise , Adulto , Contagem de Linfócito CD4 , Feminino , Infecções por HIV/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Carga Viral
19.
Rev Soc Bras Med Trop ; 34(5): 459-66, 2001.
Artigo em Português | MEDLINE | ID: mdl-11600912

RESUMO

In order to evaluate some aspects of the Chagas' disease on urban population of the 12 municipalities of the sanitarian district of Rio Verde, Mato Grosso do Sul state, on 1998 we carried out a serological survey investigating anti-T. cruzi IgG by indirect immunofluorescence on blood samples collected on filter paper of 14,709 resident persons, confirmed by indirect hemagglutination and ELISA in serum. The parasitaemia was evaluated by indirect xenodiagnosis on 134 chagasic patients and the morbidity evaluated by anamnesis, physical exam and electrocardiogram in 191 pairs of chagasic/non-chagasic patients. The seropositivity was 1.83% (0.93% in autochtonous, 5.01% in alochtonous), xenodiagnosis was positive in 17.2% of the individuals (12.3% in autochtonous, 20.8% in alochtonous) and 24.6% of the seropositive patients presented chronic chagasic cardiopathy (19.1% in autochtonous, 27.8 in alochtonous). The analysis of the dates showed that alochtonous population is the main responsible for presence of the infection and morbidity of Chagas disease in the studied area.


Assuntos
Doença de Chagas/epidemiologia , Adolescente , Adulto , Brasil/epidemiologia , Área Programática de Saúde , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , População Urbana
20.
Mem Inst Oswaldo Cruz ; 96(6): 777-9, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11562701

RESUMO

During the period from 1987 to 1998, 13 cases of human sporotrichosis were recorded at the Research Center Evandro Chagas Hospital (CPqHEC) in Rio de Janeiro. Two of these patients related scratch by a sick cat. During the subsequent period from July 1998 to July 2000, 66 human, 117 cats and 7 dogs with sporotrichosis were diagnosed at the CPqHEC. Fifty-two humans (78.8%) reported contact with cats with sporotrichosis, and 31 (47%) of them reporting a history of a scratch or bite. This epidemic, unprecedented in the literature, involving cats, dogs and human beings may have started insidiously before 1998.


Assuntos
Doenças do Gato/epidemiologia , Doenças Transmissíveis Emergentes/epidemiologia , Doenças do Cão/epidemiologia , Esporotricose/epidemiologia , Zoonoses/epidemiologia , Adolescente , Adulto , Idoso , Animais , Brasil/epidemiologia , Doenças do Gato/microbiologia , Gatos , Criança , Doenças Transmissíveis Emergentes/veterinária , Doenças do Cão/microbiologia , Cães , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Esporotricose/veterinária
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