Curbing the major and growing threats from invasive alien species is urgent and achievable.

Although invasive alien species have long been recognized as a major threat to nature and people, until now there has been no comprehensive global review of the status, trends, drivers, impacts, management and governance challenges of biological invasions. The Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) Thematic Assessment Report on Invasive Alien Species and Their Control (hereafter 'IPBES invasive alien species assessment') drew on more than 13,000 scientific publications and reports in 15 languages as well as Indigenous and local knowledge on all taxa, ecosystems and regions across the globe. Therefore, it provides unequivocal evidence of the major and growing threat of invasive alien species alongside ambitious but realistic approaches to manage biological invasions. The extent of the threat and impacts has been recognized by the 143 member states of IPBES who approved the summary for policymakers of this assessment. Here, the authors of the IPBES assessment outline the main findings of the IPBES invasive alien species assessment and highlight the urgency to act now.

Assessment of Human Health Impacts from Invasive Pufferfish (Attacks, Poisonings and Fatalities) across the Eastern Mediterranean.

The silver-cheeked toadfish Lagocephalus sceleratus (Gmelin 1789), and to a lesser degree the orange spotted toadfish Torquigener hypselogeneion (Bleeker, 1852), pose threats to human health from physical attacks and poisonings in the Eastern Mediterranean Sea. This study reviewed human health-related impacts resulting from these pufferfish, compiling and assessing records from online sources, the peer-reviewed literature, medical records, personal interviews, and observations across the Eastern Mediterranean in the years 2004 to 2023. A total of 198 events impacting human health were documented: 28 records of physical attacks, at least 144 non-lethal poisoning episodes, and 27 human fatalities resulting from consumption. The majority of the reported incidences occurred in Syria, Türkiye, and Lebanon. Most physical attacks occurred in summer, while most poisoning events occurred during winter. The number of recorded incidents greatly increased after 2019, especially with regard to poisonings, yet whether this is related to greater media attention, or to increased fish abundance is unclear. This is the first comprehensive study to collate findings on attacks, poisonings and fatalities caused by these pufferfish in the Mediterranean Sea, and may help in improving national health policies. We urge the continuation of national campaigns to caution residents and tourists of these species' high toxicities and potential aggressiveness.

Ecological baselines in the Eastern Mediterranean Sea shifted long before the availability of observational time series.

Native biodiversity loss and invasions by nonindigenous species (NIS) have massively altered ecosystems worldwide, but trajectories of taxonomic and functional reorganization remain poorly understood due to the scarcity of long-term data. Where ecological time series are available, their temporal coverage is often shorter than the history of anthropogenic changes, posing the risk of drawing misleading conclusions on systems' current states and future development. Focusing on the Eastern Mediterranean Sea, a region affected by massive biological invasions and the largest climate change-driven collapse of native marine biodiversity ever documented, we followed the taxonomic and functional evolution of an emerging "novel ecosystem", using a unique dataset on shelled mollusks sampled in 2005-2022 on the Israeli shelf. To quantify the alteration of observed assemblages relative to historical times, we also analyzed decades- to centuries-old ecological baselines reconstructed from radiometrically dated death assemblages, time-averaged accumulations of shells on the seafloor that constitute natural archives of past community states. Against expectations, we found no major loss of native biodiversity in the past two decades, suggesting that its collapse had occurred even earlier than 2005. Instead, assemblage taxonomic and functional richness increased, reflecting the diversification of NIS whose trait structure was, and has remained, different from the native one. The comparison with the death assemblage, however, revealed that modern assemblages are taxonomically and functionally much impoverished compared to historical communities. This implies that NIS did not compensate for the functional loss of native taxa, and that even the most complete observational dataset available for the region represents a shifted baseline that does not reflect the actual magnitude of anthropogenic changes. While highlighting the great value of observational time series, our results call for the integration of multiple information sources on past ecosystem states to better understand patterns of biodiversity loss in the Anthropocene.

Including a diverse set of voices to address biological invasions.

Inclusivity is fundamental to progress in understanding and addressing the global phenomena of biological invasions because inclusivity fosters a breadth of perspectives, knowledge, and solutions. Here, we report on how the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) assessment on invasive alien species (IAS) prioritized inclusivity, the benefits of this approach, and the remaining challenges.

Global marine biosecurity and ship lay-ups: intensifying effects of trade disruptions.

Recent global trade disruptions, due to blockage of the Suez Canal and cascading effects of COVID-19, have altered the movement patterns of commercial ships and may increase worldwide invasions of marine non-indigenous species. Organisms settle on the hulls and underwater surfaces of vessels and can accumulate rapidly, especially when vessels remain stationary during lay-ups and delays. Once present, organisms can persist on vessels for long-periods (months to years), with the potential to release propagules and seed invasions as ships visit ports across the global transportation network. Shipborne propagules also may be released in increasing numbers during extended vessel residence times at port or anchor. Thus, the large scale of shipping disruptions, impacting thousands of vessels and geographic locations and still on-going for over two years, may elevate invasion rates in coastal ecosystems in the absence of policy and management efforts to prevent this outcome. Concerted international and national biosecurity actions, mobilizing existing frameworks and tools with due diligence, are urgently needed to address a critical gap and abate the associated invasion risks.

Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish.

Twelve microsatellite loci, obtained by whole genome sequencing approach, were developed and validated for the rhizostomatid jellyfish Rhopilema nomadica, the most pernicious invasive species in the Mediterranean Sea. A sample of 40 specimens collected at six locations along the Mediterranean coast of Israel were genotyped and all loci presented suitable outcomes to population genetic studies, revealing 5-19 alleles/locus with clean and reproducible amplifications. Observed and expected heterozygosity ranged 0.0.353 to 0.971 and 0.335 to 0.870, respectively, and the fixation index (inbreeding coefficient) and the polymorphic information content (PIC) ranged between - 0.190 and 0.240 and 0.32 to 0.858, respectively. The new set of microsatellite loci will be used to study long-term changes in the population genetic parameters of this invasive species.

Trends in the detection of aquatic non-indigenous species across global marine, estuarine and freshwater ecosystems: A 50-year perspective.

Aim: The introduction of aquatic non-indigenous species (ANS) has become a major driver for global changes in species biogeography. We examined spatial patterns and temporal trends of ANS detections since 1965 to inform conservation policy and management. Location: Global. Methods: We assembled an extensive dataset of first records of detection of ANS (1965-2015) across 49 aquatic ecosystems, including the (a) year of first collection, (b) population status and (c) potential pathway(s) of introduction. Data were analysed at global and regional levels to assess patterns of detection rate, richness and transport pathways. Results: An annual mean of 43 (±16 SD) primary detections of ANS occurred-one new detection every 8.4 days for 50 years. The global rate of detections was relatively stable during 1965-1995, but increased rapidly after this time, peaking at roughly 66 primary detections per year during 2005-2010 and then declining marginally. Detection rates were variable within and across regions through time. Arthropods, molluscs and fishes were the most frequently reported ANS. Most ANS were likely introduced as stowaways in ships' ballast water or biofouling, although direct evidence is typically absent. Main conclusions: This synthesis highlights the magnitude of recent ANS detections, yet almost certainly represents an underestimate as many ANS go unreported due to limited search effort and diminishing taxonomic expertise. Temporal rates of detection are also confounded by reporting lags, likely contributing to the lower detection rate observed in recent years. There is a critical need to implement standardized, repeated methods across regions and taxa to improve the quality of global-scale comparisons and sustain core measures over longer time-scales. It will be fundamental to fill in knowledge gaps given that invasion data representing broad regions of the world's oceans are not yet readily available and to maintain knowledge pipelines for adaptive management.

The potential of large rafting objects to spread Lessepsian invaders: the case of a detached buoy.

A diverse and abundant fouling community dominated by Lessepsian non-indigenous species was identified on a 13.5-m-long steel buoy stranded on the Israeli coast but originating from Port Said, at the Mediterranean entrance of the Suez Canal, Egypt. The molluscan community was sampled quantitatively by scraping. Three quarters of the individuals and more than half of the species were non-indigenous. Among the latter, a mytilid bivalve, Gregariella cf. ehrenbergi, was first recorded in the Mediterranean Sea on the basis of these samples, suggesting that the full consideration of all potential vectors can contribute to non-indigenous species detection. Large floating objects in coastal waters, such as buoys, are particularly suitable for colonization by Lessepsian species because hard substrates, and artificial ones in particular, are highly susceptible to the establishment of non-indigenous species. Moreover, their size and persistence enable the development of abundant and mature fouling communities that can disseminate propagules as eggs and larvae over long distances and for extended periods if detached. This report highlights the potential for large rafting debris as a vector of the spread of non-indigenous biota within the Mediterranean Sea.

Rare and new East African leucosiid crabs.

A small collection of East African leucosiid crabs, preserved in the crustacean collection at the Museo di Storia Naturale dell'Università di Firenze, Italy, was examined. Of these, one, Cryptocnemus grandidieri A. Milne-Edwards 1865, collected in 1864 by Alfred Grandidier in Zanzibar and known only from the single type specimen, is redescribed and illustrated. The others are new to science: one each are included in Leucisca MacLeay, 1838 and Nobiliella Komatsu Takeda, 2003. These new species are described and illustrated, and their affinities with allied taxa discussed.

New records of non-indigenous molluscs from the eastern Mediterranean Sea.

We report new findings of non-indigenous Indo-Pacific molluscs from shallow water habitats off Israel, Greece and Egypt, eastern Mediterranean Sea. The bivalves Pillucina vietnamica Zorina, 1978 and Alveinus miliaceus (Issel, 1869) were collected from sandy bottoms off Israel, whereas Gregariella cf. ehrenbergi (Issel, 1869) was recovered from a buoy originating from Port Said, Egypt, and stranded on the Israeli coast. The three species are first records for the Mediterranean Sea. Additionally, we report range extensions for several gastropods: Varicopeza pauxilla (A. Adams, 1855) is recorded from Israel, Phidiana militaris (Alder and Hancock, 1864) from southern Israel (Ashqelon), and Viriola cf. bayani Jousseaume, 1884 from Israel and Crete. Shells and valves of an unidentified lucinid bivalve morphologically distinct from any known Mediterranean species were found along the Israeli Mediterranean shore.

Validation and redescription of the hyperiidean amphipod Brachyscelus rapacoides Stephensen, 1925 (Crustacea: Amphipoda: Hyperiidea: Brachyscelidae), a new record of association with the scyphozoan jellyfish Rhopilema nomadica Galil, 1990 (Scyphozoa: Rhizostomeae: Rhizostomatidae) in the Mediterranean Sea.

The hyperiidean amphipod Brachyscelus rapacoides Stephensen, 1925 is recorded from the scyphozoan jellyfish Rhopilema nomadica Galil, 1990, a new record of association for the genus Brachyscelus, as well as the first record of hyperiid infestation of a non-indigenous scyphozoan host. Because of some past confusion concerning the status of B. rapacoides and the closely related species B. rapax (Claus, 1871) a redescription of B. rapacoides and molecular analysis are provided in order to validate it as a species distinguished from B. rapax.

Historical baselines in marine bioinvasions: Implications for policy and management.

The human-mediated introduction of marine non-indigenous species is a centuries- if not millennia-old phenomenon, but was only recently acknowledged as a potent driver of change in the sea. We provide a synopsis of key historical milestones for marine bioinvasions, including timelines of (a) discovery and understanding of the invasion process, focusing on transfer mechanisms and outcomes, (b) methodologies used for detection and monitoring, (c) approaches to ecological impacts research, and (d) management and policy responses. Early (until the mid-1900s) marine bioinvasions were given little attention, and in a number of cases actively and routinely facilitated. Beginning in the second half of the 20th century, several conspicuous non-indigenous species outbreaks with strong environmental, economic, and public health impacts raised widespread concerns and initiated shifts in public and scientific perceptions. These high-profile invasions led to policy documents and strategies to reduce the introduction and spread of non-indigenous species, although with significant time lags and limited success and focused on only a subset of transfer mechanisms. Integrated, multi-vector management within an ecosystem-based marine management context is urgently needed to address the complex interactions of natural and human pressures that drive invasions in marine ecosystems.

The resurrection of Charybdis (Gonioinfradens) giardi (Nobili, 1905), newly recorded from the SE Mediterranean Sea.

A single adult specimen of Gonioinfradens giardi, a portunid crab known from the Red Sea, Gulf of Oman and Arabian Gulf, was recently collected off the southern Israeli coast, in the southeastern Mediterranean Sea. Morphological characters, as well as molecular analyses based on the mitochondrial barcoding gene cytochrome oxidase sub unit I (COI), support its distinction from the widely distributed G. paucidentata. Therefore, G. giardi is reinstated as a valid species, and withdrawn from its synonymy with G. paucidentata. Previous Mediterranean records of the latter species are misidentifications and should be referred to G. giardi. The species is described, illustrated, and differentiated from its cogener.

Plankton resting stages in recent sediments of Haifa port, Israel (Eastern Mediterranean) - Distribution, viability and potential environmental consequences.

Resting stages of plankton were sampled in the surficial sediments in the port of Haifa, Israel, on the eve of a major port enlargement project. We recorded the structure of the assemblages and examined their relationship with different environments within the port. Our findings reveal a remarkably high diversity coupled with low density and the highest number of oligotrich ciliate cyst types recorded from marine sediments. Near the eutrophic and highly polluted zone of the Kishon estuary ciliates were more abundant than elsewhere in the port, whereas dinoflagellates' abundance was reduced, and these trends held true both for full and empty cysts. Some harmful or potentially toxic species, such as Scrippsiella acuminata, were widespread in the port. The toxigenic species include Alexandrium minutum, Gymnodinium uncatenatum and Lingulodinium polyedrum. Active cells of the unarmoured, bloom-forming Akashiwo sanguinea were identified in the cultures obtained from the incubated sediments.

Recommendations for developing and applying genetic tools to assess and manage biological invasions in marine ecosystems.

The European Union's Marine Strategy Framework Directive (MSFD) aims to adopt integrated ecosystem management approaches to achieve or maintain "Good Environmental Status" for marine waters, habitats and resources, including mitigation of the negative effects of non-indigenous species (NIS). The Directive further seeks to promote broadly standardized monitoring efforts and assessment of temporal trends in marine ecosystem condition, incorporating metrics describing the distribution and impacts of NIS. Accomplishing these goals will require application of advanced tools for NIS surveillance and risk assessment, particularly given known challenges associated with surveying and monitoring with traditional methods. In the past decade, a host of methods based on nucleic acids (DNA and RNA) analysis have been developed or advanced that promise to dramatically enhance capacity in assessing and managing NIS. However, ensuring that these rapidly evolving approaches remain accessible and responsive to the needs of resource managers remains a challenge. This paper provides recommendations for future development of these genetic tools for assessment and management of NIS in marine systems, within the context of the explicit requirements of the MSFD. Issues considered include technological innovation, methodological standardization, data sharing and collaboration, and the critical importance of shared foundational resources, particularly integrated taxonomic expertise. Though the recommendations offered here are not exhaustive, they provide a basis for future intentional (and international) collaborative development of a genetic toolkit for NIS research, capable of fulfilling the immediate and long term goals of marine ecosystem and resource conservation.

Grandidierella bonnieroides Stephensen, 1948 (Amphipoda, Aoridae)-first record of an established population in the Mediterranean Sea.

The first record in the Mediterranean Sea of the invasive aorid amphipod crustacean Grandidierella bonnieroides is presented. A widespread circumtropical species, recorded off the Saudi coast of the Arabian Gulf, the Red Sea and the Suez Canal, it may have been introduced into the Mediterranean through the Suez Canal. This tube-builder species of soft bottoms recently established a population in the polluted Haifa Bay, Israel. Further, this is the first Mediterranean record of the genus.

Leucosiid crabs from Papua New Guinea, with descriptions of eight new species (Crustacea: Decapoda: Brachyura).

Twenty-five species of leucosiid crabs are reported from Madang Province, Papua New Guinea. Of these, seven are new to science: two each are included in Alox Tan & Ng, 1995 and Tanaoa Galil, 2003, and one each in Ryphila Galil, 2009, Seulocia Galil, 2005, and Urnalana Galil, 2005. Fifteen additional species are new records for Papua New Guinea: Alox rugosum (Stimpson, 1858), Ancylodactyla nana (Zarenkov, 1990), Arcania heptacantha De Man, 1907, Heterolithadia fallax (Henderson, 1893), Hiplyra longimana (A. Milne Edwards, 1874), Myra curtimana Galil, 2001, M. digitata Galil 2004, Nursilia dentata Bell, 1855, Oreotlos etor Tan & Richer de Forges, 1993, Parilia major Sakai, 1961, Raylilia coniculifera Galil, 2001, R. uenoi (Takeda, 1995), Toru pilus (Tan, 1996), Urashima pustuloides (Sakai, 1961) and Leucosia rubripalma Galil, 2003. The new species are described and illustrated, and their affinities with allied taxa discussed. Colour photographs are provided for 20 species.

Recommendations on standardizing lists of marine alien species: Lessons from the Mediterranean Sea.

Analyses of marine alien species based on national/regional datasets are of paramount importance for the success of regulation on the prevention and management of invasive alien species. Yet in the extant data systems the criteria for the inclusion of records are seldom explicit, and frequently inconsistent in their definitions, spatial and temporal frames and comprehensiveness. Agreed-upon uniform guiding principles, based on solid and transparent scientific criteria, are therefore required in order to provide policy makers with validated and comparable data. Following a meta-analysis on the records of marine alien species in the Mediterranean Sea, we recommend a judicious approach to compiling the data. Here, three categories of uncertainty were identified: species' taxonomic identification, species' actual occurrence in the area, and its status as an alien. In proposing guiding principles to standardize such datasets, we aim to encourage discourse on logical, standardized and transparent criteria to substantiate records of alien species.

A checklist of the deep sea fishes of the Levant Sea, Mediterranean Sea.

We list sixty five fish species collected at depths greater than 500 m in the Levant Basin, including 10 depth records. The Levantine bathyal ichthyofauna is characterized by its eurybathy, with an upper bathymetric boundary that permitted penetration of the shallow Gibraltar and Siculo-Tunisian sills, and a much lower bathymetric boundary than recorded for conspecifics elsewhere. The opportunistic and resilient ichthyofauna re-colonized recently the deep-sea following the last anoxic event (~ 6 kyr), forming assemblages notably distinct from those in the western Mediterranean. The exploration and production of deep seabed hydrocarbons have raised the specter of severe direct impacts to the deep habitats. There is an urgent need for documenting the full extent of deep-sea biodiversity, and for providing information for the development of competent and pragmatic management plans and effective conservation policies.

An additional record of Kyphosus vaigiensis (Quoy & Gaimard, 1825) (Osteichthyes, Kyphosidae) from Sicily clarifies the confused situation of the Mediterranean kyphosids.

The lowfin chub, Kyphosus vaigiensis, is reported for the first time off Favignana Island, Sicily, central Mediterranean Sea. The specimen was identified on the basis of morphometric and meristic characters as well as mitochondrial DNA sequences (COI and 16S-rDNA). Two, perhaps three, Kyphosus species-K. bigibbus, K. sectatrix and K. vaigiensis-have been occasionally recorded in the Mediterranean. These species occur both in the Atlantic and Indo-Pacific regions but it is likely they entered the Mediterranean through the Strait of Gibraltar. However, it is unclear whether they have established reproductive native populations in the Mediterranean.