Predicting microbial crude protein synthesis in cattle from intakes of dietary energy and crude protein.

Accurate predictions of microbial crude protein (MCP) synthesis are needed to predict metabolizable protein supply in ruminants. Since 1996, the National Academies of Sciences, Engineering, and Medicine series on beef cattle nutrient requirements has used the intake of total digestible nutrients (TDN) to predict ruminal MCP synthesis. Because various tabular energy values for feeds are highly correlated, our objective was to determine whether intakes of digestible energy (DE), metabolizable energy (ME), and net energy for maintenance (NEm) could be used as predictors of MCP synthesis in beef cattle. A published database of 285 treatment means from experiments that evaluated MCP synthesis was updated with 50 additional treatment mean observations. When intakes of TDN, fat-free TDN, DE, ME, NEm, dry matter, organic matter, crude protein (CP), ether extract, neutral detergent fiber, and starch were used in a stepwise regression analysis to predict MCP, only intakes of DE and CP met the P < 0.10 criterion for entry into the model. Mixed-model regression analyses were used to adjust for random intercept and slope effects of citations to evaluate intake of DE alone or in combination with CP intake as predictors of MCP synthesis, and the intakes of TDN, ME, and NEm as alternatives to DE intake. Similar precisions in predicting MCP synthesis were obtained with all measures of energy intake (CV = root mean square error [RMSE] as a percentage of the overall mean MCP varied from 9% to 9.67%), and adding CP intake to statistical models increased precision (CV ranged from 8.43% to 9.39%). Resampling analyses were used to evaluate observed vs. predicted values for the various energy intake models with or without CP intake, as well as the TDN-based equation used in the current beef cattle nutrient requirements calculations. The coefficient of determination, concordance correlation coefficient, and RMSE of prediction as a percentage of the mean averaged 0.595%, 0.730%, and 28.6% for the four measures of energy intake, with average values of 0.630%, 0.757%, and 27.4%, respectively, for equations that included CP intake. The TDN equation adopted by the 2016 beef cattle nutrient requirements system yielded similar results to newly developed equations but had a slightly greater mean bias. We concluded that any of the measures of energy intake we evaluated can be used to predict MCP synthesis by beef cattle and that adding CP intake improves model precision.

The ability to accurately and precisely predict microbial protein synthesis is crucial in predicting the supply of metabolizable protein in beef cattle. We updated a database previously used to predict microbial crude protein (CP) supply in beef cattle. Then we evaluated the utility of using total digestible nutrients (TDN) and different measures of energy intake to predict ruminal microbial protein synthesis. Using regression analyses, we found that intakes of digestible and metabolizable energy and intake of net energy for maintenance were as effective as the intake of TDN as independent variables to predict microbial protein synthesis. Adding the intake of CP to the models improved the precision of predictions, and all models performed as well or better than the regression model adopted by the 2016 nutrient requirement system for beef cattle.

Effects of tannic acid on growth performance, carcass characteristics, digestibility, nitrogen volatilization, and meat lipid oxidation of steers fed steam-flaked corn-based finishing diets.

Effects of a tannic acid blend (ByPro; Silvateam USA, Ontario, CA) added to steam-flaked corn-based fishing diets on beef cattle growth performance, carcass characteristics, nutrient digestibility, fecal N volatilization, and meat lipid oxidation were evaluated. Steers ( = 144; 349 ± 25 kg initial BW) were blocked by initial BW and assigned randomly to 1 of 3 treatments with 12 pens/treatment and 4 steers/pen and fed ad libitum. Treatments included a control (CON; no ByPro) and ByPro fed at 30 or 60 g DM/steer daily (30-ByPro and 60-ByPro, respectively). Pen fecal samples were collected 7 d after cattle were shipped to slaughter for estimation of N volatilization. Strip loins were aged for 21 d for evaluation of color and antioxidant activity. Intake quadratically increased ( = 0.05) from d 0 to 35, whereas linear trends were observed for increased DMI from d 0 to 105 and d 0 to slaughter ( = 0.07 and = 0.06, respectively), resulting in a 3.7% greater overall DMI for 60-ByPro than for CON. No differences were detected for carcass-adjusted ADG ( = 0.65) or G:F ( = 0.17). Carcass characteristics including HCW ( = 0.52), fat thickness ( = 0.32), LM area ( = 0.57), quality grade ( = 0.44), yield grade ( = 0.29), and percentage of condemned livers ( = 0.13) were not affected by treatments. Apparent total tract digestibility of starch linearly decreased tendency ( = 0.03) with increasing ByPro dose, whereas tends for a linear decrease ( = 0.09) in CP and a quadratic increase ( = 0.09) in OM digestibility were observed. No effects of treatment ( ≥ 0.39) were noted for fecal N volatilization. An increase ( < 0.01) in metmyoglobin in strip loin steaks was observed with ByPro inclusion. Oxymyoglobin decreased ( < 0.01) as display day progressed, except on d 5, at which time CON and 30-ByPro steaks had lower proportions than 60-ByPro steaks. Only subtle changes in discoloration ratio and deoxymyoglobin were observed, whereas no effects ( ≥ 0.43) for pH or thiobarbituric acid reactive substances were noted. Feeding ByPro increased DMI during the first half of the feeding period without negatively affecting gain efficiency; however, fecal N retention was not altered by ByPro. ByPro did not negatively affect meat quality or carcass characteristics, and it did not seem to affect retail meat antioxidant activity.

Nutritional recommendations of feedlot consulting nutritionists: The 2015 New Mexico State and Texas Tech University survey.

The 2015 feedlot consulting nutritionist survey is a collaborative project between New Mexico State University and Texas Tech University that focuses on summarizing the professional practices of consulting feedlot nutritionists and updates a 2007 survey. Forty-nine consulting feedlot nutritionists were asked to participate, of which 24 completed the survey. The nutritionists surveyed service over 14,000,000 cattle annually and were representatives from individual consulting practices (54.2%), corporate cattle feeding companies (20.8%), corporate feed manufacturing companies (20.8%), or a combination of consulting practices (4.2%). The survey was completed using a web-based survey tool and contained 101 questions that were divided into sections regarding general information about the consulting practice; general cattle management; receiving cattle management, diet adaption; mixers, feed mills, and feeding management; grains and grain processing; grain by-product use; roughage use; information about supplements and microingredients; liquid feed use; nutrient formulation; feed additive use; and information used as a basis for nutritional recommendations. In most cases, the results of the current survey were similar to those reported for the 2007 survey, with a few notable exceptions such as shifts in cattle numbers and preferences for specific feedstuffs. The present study introduced a number of new questions not included in the 2007 survey that focused on management strategies used in the receiving period. Data from this survey provide insight into current nutritional and management practices of consulting nutritionists and, as in past surveys, should be useful for informing national committees that make nutritional recommendations for cattle, as well as nutrition and management strategies employed within university research settings.

BOARD-INVITED REVIEW: Efficiency of converting digestible energy to metabolizable energy and reevaluation of the California Net Energy System maintenance requirements and equations for predicting dietary net energy values for beef cattle.

For the past several decades, nutrient requirement systems for beef cattle in North America have recommended that dietary ME can be calculated as dietary DE × 0.82, but considerable published data suggest a variable relationship between DE and ME. We reviewed the literature and tabulated the results of 23 respiration calorimetry studies (87 treatment mean data points), in which measurements of fecal, urinary, and gaseous energy were determined with beef cattle (bulls, steers, and heifers) and growing dairy cattle. Mixed-model regression analyses to adjust for the effects of the citation from which the data were obtained suggested a strong linear relationship between ME and DE (Mcal/kg of DM; ME = 0.9611 × DE - 0.2999; = 0.986, root mean square error [RMSE] = 0.048, < 0.001 for intercept, slope ≠ 0). Analysis of residuals from this simple linear regression equation indicated high correlations of residuals with other dietary components, and a slight increase in precision was obtained when dietary CP, ether extract, and starch (% of DM) concentrations were included in a multiple linear regression equation (citation-adjusted = 0.992, RMSE = 0.039). Using the simple linear relationship, we reevaluated the original data used to develop the California Net Energy System (CNES) for beef cattle by recalculating ME intake and heat production and regressing the logarithm of heat production on ME intake (both per BW, kg daily). The resulting intercept and slope of the recalculated data did not differ ( ≥ 0.34) from those reported for the original analyses of the CNES data, suggesting that use of the linear equation for calculating ME concentration was consistent with NEm and NEg values as derived in the CNES. Nonetheless, because the cubic equations recommended by the NRC to calculate dietary NEm and NEg from ME were based on conversion of DE to ME using 0.82, these equations were mathematically recalculated to account for the linear relationship between DE and ME. Overall, our review and analyses suggested that there is a strong linear relationship between DE and ME, which seems to be consistent across a wide range of dietary conditions, cattle types, and levels of intake. Applying this linear relationship to predict ME concentrations agreed with the original CNES calculations for NE requirements, thereby allowing the development of new equations for predicting dietary NEm and NEg values from ME.

Biofuel feedstock and blended coproducts compared with deoiled corn distillers grains in feedlot diets: Effects on cattle growth performance, apparent total tract nutrient digestibility, and carcass characteristics.

Crossbred steers (British × Continental; = 192; initial BW 391 ± 28 kg) were used to evaluate the effects of feeding ethanol coproducts on feedlot cattle growth performance, apparent nutrient digestibility, and carcass characteristics. Steers were blocked by initial BW and assigned randomly to 1 of 6 dietary treatments within block. Treatments (replicated in 8 pens with 4 steers/pen) included 1) control, steam-flaked corn-based diet (CTL), 2) corn dried distillers grains with solubles (DGS; DRY-C), 3) deoiled corn dried DGS (DRY-CLF), 4) blended 50/50 corn/sorghum dried DGS (DRY-C/S), 5) sorghum dried DGS (DRY-S), and 6) sorghum wet DGS (WET-S). Inclusion of DGS was 25% (DM basis). The DGS diets were isonitrogenous, CTL was formulated for 13.5% CP, and all diets were balanced for ether extract. Final shrunk BW, ADG, and DMI did not differ among CTL and DGS treatments ( ≥ 0.19). Overall G:F did not differ from CTL for DRY-C, DRY-CLF, or WET-S ( ≥ 0.12); however, G:F was 9.6% less for DRY-S compared with CTL ( < 0.01) and tended ( = 0.09) to be less for DRY-C/S than CTL. For grain source, ADG and G:F were less for DRY-S vs. DRY-C ( < 0.05), but blending DRY-C/S tended ( = 0.07) to increase ADG and increased ( = 0.05) carcass-adjusted G:F vs. DRY-S. For WET-S, final BW and ADG were greater ( < 0.05), and G:F tended ( = 0.06) to be greater than for DRY-S. There was no difference in ADG, DMI, or G:F of steers fed DRY-C vs. DRY-CLF ( ≥ 0.35). Apparent DM and OM digestibility did not differ for CTL, DRY-C, DRY-CLF, and WET-S ( ≥ 0.30) but were lower for DRY-C/S and DRY-S ( < 0.05). Nutrient digestibility was lower for DRY-S vs. DRY-C ( < 0.01), but apparent digestibility of OM, DM, NDF, ADF, CP, ether extract, and starch were increased ( < 0.01) for DRY-C/S vs. DRY-S. Although starch digestibility did not differ between DRY-S and WET-S ( 0.18), digestibility of other measured nutrients was greater for WET-S vs. DRY-S ( < 0.01). Ether extract digestibility was greater for DRY-CLF vs. DRY-C ( < 0.05). Carcass weight, dressing percent, and marbling score did not differ between CTL and DGS diets ( ≥ 0.23). For DRY-S, HCW was lower than for DRY-C ( = 0.02); however, compared with DRY-S, HCW tended to be greater for DRY-C/S ( = 0.10) and WET-S ( = 0.07). At a moderately high (25% DM) inclusion, blending C/S or feeding WET-S resulted in cattle growth performance and carcass characteristics similar to those of CTL and corn-based coproducts.

Evaluation of in vitro models for predicting acidosis risk of barley grain in finishing beef cattle.

Our objective was to develop a model to predict the acidosis potential of barley based on the in vitro batch culture incubation of 50 samples varying in bulk density, starch content, processing method, growing location, and agronomic practices. The model was an adaptation of the acidosis index (calculated from a combination of in situ and in vitro analyses and from several components of grain chemical composition) developed in Australia for use in the feed industry to estimate the potential for grains to increase the risk of ruminal acidosis. Of the independent variables considered, DM disappearance at 6 h of incubation (DMD6) using reduced-strength (20%) buffer in the batch culture accounted for 90.5% of the variation in the acidosis index with a root mean square error (RMSE) of 4.46%. To evaluate our model using independent datasets (derived from previous batch culture studies using full-strength [100%] buffer), we performed another batch culture study using full-strength buffer. The full-strength buffer model using in vitro DMD6 (DMD6-FS) accounted for 66.5% of the variation in the acidosis index with an RMSE of 8.30%. When the new full-strength buffer model was applied to 3 independent datasets to predict acidosis, it accounted for 20.1, 28.5, and 30.2% of the variation in the calculated acidosis index. Significant ( < 0.001) mean bias was evident in 2 of the datasets, for which the DMD6 model underpredicted the acidosis index by 46.9 and 5.73%. Ranking of samples from the most diverse independent dataset using the DMD6-FS model and the Black (2008) model (calculated using in situ starch degradation) indicated the relationship between the rankings using Spearman's rank correlation was negative (ρ = -0.30; = 0.059). When the reduced-strength buffer model was used, however, there were similarities in the acidosis index ranking of barley samples by the models as shown by the result of a correlation analysis between calculated (using the Australian model) and predicted (using the reduced-strength buffer DMD6 model) acidosis index (ρ = 0.67; < 0.001). Results suggest that our model, which is based on a reduced-strength buffer in vitro DMD6, has the potential to predict acidosis risk and can rank barley samples based on their acidotic risk. Nonetheless, the model would benefit from further refinement by expanding the database.

Predicting microbial protein synthesis in beef cattle: relationship to intakes of total digestible nutrients and crude protein.

Prediction of microbial CP (MCP) synthesis in the rumen is an integral part of the MP system. For the NRC beef model, MCP is calculated as 0.13 multiplied by TDN intake (TDNI), with adjustment for physically effective NDF (peNDF) concentrations less than 20%. Despite its application for nearly 2 decades, MCP predictions using this approach have not been extensively evaluated. We assembled a database of 285 treatment means from 66 published papers using beef cattle and dairy or dairy × beef crossbred steers, fed diets with a wide range of TDN, CP, and ether extract (EE) concentrations, in which MCP synthesis was measured. Fat-free TDN (FFTDN) concentration was calculated by subtracting 2.25 × percent EE from the TDN concentration. Based on initial model selection procedures indicating that DMI and concentrations of TDN, FFTDN, and CP were significantly (P < 0.04) related to MCP synthesis, linear and quadratic effects of TDNI and FFTDN intake (FFTDNI) and CP intake (CPI) were considered as potential independent variables. Mixed model regression methods were used to fit 1-, 2-, and 3-independent-variable models based on either TDNI or FFTDNI (e.g., TDNI only, TDNI and CPI, and TDNI, CPI, and the quadratic effect of TDNI; or FFTDNI only, FFTDNI and CPI, and FFTDNI, CPI, and the quadratic effect of FFTDNI). True ruminal OM digested (TROMD; g/d) was highly related (r(2) = 0.84 using citation-adjusted data) to MCP synthesis. Similarly, both TDNI and FFTDNI were highly related to citation-adjusted TROMD (r(2) > 0.96) and MCP synthesis (r(2) > 0.89). Models with FFTDNI were slightly more precise with slightly smaller prediction errors than those with TDNI. Randomly dividing the citations into Development (60%) and Evaluation (40%) data sets indicated that models such as those derived from the overall database accounted for 46 to 56% of the variation in MCP synthesis, with neither mean nor linear bias (P ≥ 0.26). In contrast, calculating MCP as 0.13 × TDNI, with or without adjustment for peNDF concentration, resulted in overprediction of MCP (P < 0.001 for both mean and linear bias). Cross-validation using 5,000 randomly drawn training and testing data sets yielded results similar to the Development/Evaluation approach. Recommended equations are provided, but the errors of prediction associated with these empirical regression equations were on the order of 25 to 30% of the mean MCP.

Predicting dry matter intake by growing and finishing beef cattle: evaluation of current methods and equation development.

The NRC (1996) equation for predicting DMI by growing-finishing beef cattle, which is based on dietary NEm concentration and average BW(0.75), has been reported to over- and underpredict DMI depending on dietary and animal conditions. Our objectives were to 1) develop broadly applicable equations for predicting DMI from BW and dietary NEm concentration and 2) evaluate the predictive value of using NE requirements and dietary NE concentrations to determine the DMI required (DMIR) by feedlot cattle. Two new DMI prediction equations were developed from a literature data set, which represented treatment means from published experiments from 1980 to 2011 that covered a wide range of dietary NEm concentrations. Dry matter intake predicted from the 2 new equations, which were based on NEm concentration and either the ending BW for a feeding period or the DMI per unit of average BW (End BW and DMI/BW, respectively), accounted for 61 and 58% of the variation in observed DMI, respectively, vs. 48% for the 1996 NRC equation. When validated with 4 independent data sets that included 7,751 pen and individual observations of DMI by animals of varying BW and feeding periods of varying length, DMI predicted by the 1996 NRC equation, the End BW and DMI/BW equations, and the DMIR method accounted for 13.1 to 82.9% of the variation in observed DMI, with higher r(2) values for 2 feedlot pen data sets and lower values for pen and individual data sets that included animals on lower-energy, growing diets as well as those in feedlot settings. The DMIR method yielded the greatest r(2) values and least prediction errors across the 4 data sets; however, mean biases (P < 0.01) were evident for all the equations across the data sets, ranging from as high as 1.01 kg for the DMIR method to -1.03 kg for the 1996 NRC equation. Negative linear bias was evident in virtually all cases, suggesting that prediction errors changed as DMI increased. Despite the expanded literature database for equation development, other than a trend for lower standard errors of prediction with the DMI/BW equation, the 2 new equations did not offer major advantages over the 1996 NRC equation when applied to the validation data sets. Because the DMIR approach accounted for the greatest percentage of variation in observed DMI and had the least root mean square error values in all data sets evaluated, this approach should be considered as a means of predicting DMI by growing-finishing beef cattle.

Interactive effects of bulk density of steam-flaked corn and concentration of Sweet Bran on feedlot cattle performance, carcass characteristics, and apparent total tract nutrient digestibility.

Two hundred twenty-four steers (initial BW = 363 ± 1.57 kg) were used in a 2 × 3 + 1 factorial arrangement of treatments to evaluate the interactive effects of concentration of wet corn gluten feed (WCGF) and bulk density (BD) of steam-flaked corn (SFC) on feedlot performance, carcass characteristics, and apparent total tract digestibility. Diets consisted of 0, 15, or 30% WCGF (DM basis) with a BD of SFC at 283 or 360 g/L. The additional treatment consisted of 15% WCGF, SFC at 283 g/L, and a 6% inclusion of alfalfa hay vs. 9% for all other treatments. Steers were fed once daily for an average of 163 d. During a 5-d digestion period, DMI was measured, and fecal samples were collected for measurement of nutrient digestibility using dietary acid insoluble ash as a marker. There were few WCGF × BD interactions for feedlot performance, carcass characteristics, and digestibility. Similarly, contrasts between the treatment containing 15% WCGF/360 g/L SFC and 15% WCGF/360 g/L with 6% hay yielded few differences for performance and carcass data. Final BW responded quadratically (P ≤ 0.02) to WCGF inclusion and showed increased (P ≤ 0.007) BW for greater BD. As WCGF inclusion increased, G:F and calculated NE values (P ≤ 0.03) decreased quadratically. Steers consuming 360 g/L SFC had greater (P < 0.05) G:F than those fed 283 g/L SFC. Marbling score, HCW, 12th-rib fat thickness, and calculated yield grade increased quadratically (P ≤ 0.04) with increased inclusion of WCGF. Percentage of cattle grading premium Choice or greater responded quadratically (P = 0.04) to WCGF concentration. Increasing BD increased (P ≤ 0.01) HCW, dressing percent, marbling score, and 12th-rib fat thickness and decreased calculated yield grade and percentage of cattle grading Select; however, lower BD tended (P = 0.09) to increase LM area. Intake of DM, OM, CP, and NDF and fecal output during the digestibility period increased linearly (P ≤ 0.01) with increasing WCGF, and greater BD increased (P ≤ 0.04) intake of DM, OM, starch, and CP. Starch digestibility decreased quadratically (P = 0.008) as WCGF increased; however, digestibility of CP and NDF increased (P ≤ 0.02) linearly as WCGF increased. The 283 g/L BD increased (P ≤ 0.02) starch and CP digestibility compared with 360 g/L. These data suggest that increasing WCGF in feedlot diets with a greater BD of SFC can increase performance and carcass characteristic, but it might not be ideal for starch digestibility.

Effects of bulk density of steam-flaked corn in diets containing wet corn gluten feed on feedlot cattle performance, carcass characteristics, apparent total tract digestibility, and ruminal fermentation.

The effects of varying bulk density of steam-flaked corn (SFC) in diets containing wet corn gluten feed (WCGF; Sweet Bran; Cargill Corn Milling, Blair, NE) have not been defined. In Exp. 1, yearling steers (n = 108; initial BW = 367 ± 1.18 kg) were housed in 27 pens (4 steers/pen) and received 1 of 3 different SFC bulk density treatments in a randomized complete block design. Bulk density treatments were 283, 335, or 386 g/L SFC in diets containing 25% WCGF (% of DM). Steers were fed once daily to provide ad libitum access to feed for an average of 163 d. For a 5-d period before d 70 of the experiment, DMI was measured, and fecal samples were collected from each pen for measurement of nutrient digestibility using dietary acid insoluble ash as a marker. Varying bulk densities of SFC did not affect (P ≥ 0.233) overall DMI, ADG, or G:F on a live- or carcass-adjusted basis. Dressing percent and LM area increased linearly (P ≤ 0.05) as bulk density increased, but other carcass traits were not affected by treatments. Intake of DM, OM, and CP during the 5-d digestion phase did not differ among bulk densities; however, starch intake increased linearly (P = 0.004) as bulk density of SFC increased. Digestibility of DM, OM, and CP tended (P ≤ 0.065) to decrease and starch digestibility decreased (P = 0.002) linearly as bulk density of SFC increased. In Exp. 2, a 3 × 3 Latin square design was used for collection of ruminal fluid from 3 ruminally cannulated Jersey steers adapted to the same diets used in Exp. 1. Bulk density did not affect NH3 concentrations, VFA molar proportions, ruminal fluid osmolality, and IVDMD of the diets. Total gas production increased linearly (P = 0.003) as bulk density of SFC increased from 283 to 335 g/L, but it decreased (P = 0.002) at 386 g/L. Present data suggest that bulk density can be increased up to 386 g/L in finishing diets containing 25% (DM basis) WCGF without affecting cattle performance and with limited effects on ruminal fermentation; however, digestibility of starch seemed to be affected negatively by increased bulk density in these diets.

Effects of dietary concentration of wet distillers grains on performance by newly received beef cattle, in vitro gas production and volatile fatty acid concentrations, and in vitro dry matter disappearance.

Three studies were designed to evaluate effects of wet distillers grains with solubles (WDGS) on health and performance of newly received beef cattle, in vitro gas production, molar proportions and total concentrations of VFA, and IVDMD. In Exp. 1 and 2, 219 (BW = 209 kg, SE = 2.2 kg; Exp. 1) and 200 beef steers (BW = 186 kg, SE = 3.2 kg; Exp. 2) were used in randomized complete block design receiving studies. The 4 dietary treatments (DM basis) were a 65% concentrate, steam-flaked corn (SFC)-based receiving diet without WDGS (CON) or diets that contained 12.5, 25.0, or 37.5% WDGS. There were no differences among the 4 receiving diets in BW (P ≥ 0.61), ADG (P ≥ 0.75), DMI (P ≥ 0.27), and G:F (P ≥ 0.35), or in the proportion of cattle treated for morbidity from bovine respiratory disease in either of the 2 experiments. In Exp. 3, in vitro methods were used to determine the effects of WDGS on IVDMD, total gas production, and molar proportions and total concentrations of VFA. Substrates used for the incubations contained the same major components as the diets used in Exp. 1, with ruminal fluid obtained from steers fed a 60% concentrate diet. Total gas production was less (P = 0.03) for the average of the 3 WDGS substrates than for CON, with a linear decrease (P = 0.01) in total gas production as WDGS concentration increased in the substrates. In contrast to gas production, IVDMD was greater for the average of the 3 WDGS concentrations vs. CON (P ≤ 0.05) at 6 and 12 h and increased (P ≤ 0.02) with increasing WDGS concentration at 6 (linear and quadratic) and 12 h (linear) of incubation. At 48 h, there was a quadratic effect (P = 0.05) on IVDMD, with the greatest value for 25% WDGS. Molar proportion of butyrate increased linearly (P < 0.01) as the concentration of WDGS increased in the substrate, and the average of the 3 substrates containing WDGS had a greater proportion of butyrate (P = 0.03) than CON. Performance data from Exp. 1 and 2 indicate that including WDGS in the SFC-based diets for newly received cattle can be an effective at concentrations up to 37.5% of the DM. In vivo measurements are needed to corroborate the in vitro fermentation changes noted with addition of WDGS.

Effects of roughage source and distillers grain concentration on beef cattle finishing performance, carcass characteristics, and in vitro fermentation.

Two experiments were conducted to evaluate the effects of wet distillers grains plus solubles (DG) and roughage source on finishing cattle performance, carcass characteristics, and in vitro fermentation. In Exp. 1, crossbred beef steers (n=224, initial BW=349 kg) were used in a randomized complete block design with a 2 × 3 + 1 factorial arrangement of treatments. Experimental diets were a standard steam-flaked corn (SFC)-based control (no DG and 10% alfalfa hay), and either 15 or 30% DG (DM basis) with roughage sources of alfalfa hay (15-AH and 30-AH), Coastal bermudagrass hay (15-BG and 30-BG), or sorghum silage (15-SS and 30-SS). Within each DG concentration, roughages provided an equivalent percentage of NDF to 7.5% AH. Steers consuming 15% DG had greater (P < 0.04) final BW, ADG, and G:F than those fed 30% DG. Feeding AH as the roughage source with DG resulted in decreased final shrunk BW and ADG (P < 0.02) compared with BG and SS. Feeding SS as the roughage source decreased (P=0.01) G:F relative to BG. Hot carcass weight was greater (P < 0.01) for steers consuming 15 vs. 30% DG, tended to be least for diets with AH as the roughage source (P=0.06), and did not differ for the control vs. the other diets (P=0.86). Control cattle had an increased (P=0.05) proportion of USDA Choice or greater carcasses compared with the average of the other treatments. In Exp. 2, the same 2 × 3 +1 factorial arrangement as in Exp. 1 was used to examine the effects of roughage source and DG on IVDMD, culture fluid osmolality, and gas production kinetics. In vitro DMD tended (P < 0.09) to be greater for BG compared with SS at 6 and 36 h of incubation and was greater for AH vs. the mean of BG and SS at 18 h (P=0.01). Culture fluid osmolality, asymptotic maximal gas production, fractional rate of gas production, and lag time of gas production did not differ among treatments (P > 0.14). Overall, feeding 15% DG in SFC-based diets increased ADG, BW, and HCW relative to 30% DG. In addition, feeding AH tended to decrease ADG, final BW, and HCW relative to the other 2 roughage sources, whereas BG improved G:F over SS. These data suggest that including the smaller amount of DG and BG as the roughage source resulted in improved performance relative to other combinations, and that substituting roughages on the basis of equivalent NDF concentration might not be ideal for optimizing performance when feeding SFC-based finishing diets that contain DG.

Performance of finishing beef steers in response to anabolic implant and zilpaterol hydrochloride supplementation.

Our objectives were to evaluate the dose/payout pattern of trenbolone acetate (TBA) and estradiol-17ß (E(2)) implants and feeding of zilpaterol hydrochloride (ZH) on performance and carcass characteristics of finishing beef steers. A randomized complete block design was used with a 3 × 2 factorial arrangement of treatments. British × Continental steers (n = 168; initial BW = 362 kg) were blocked by BW and allotted randomly to 42 pens (7 pens/treatment; 6 pens/block; 4 steers/pen). The main effects of treatment were implant [no implant (NI); Revalor-S (REV-S; 120 mg of TBA + 24 mg of E(2)); and Revalor-XS (REV-X; 200 mg of TBA + 40 mg of E(2))] and ZH (0 or 8.3 mg/kg of DM for 20 d with a 3-d withdrawal before slaughter). Blocks were split into 2 groups, and block groups were fed for either 153 or 174 d. No implant × ZH interactions were noted for cumulative performance data. Overall, shrunk final BW (567, 606, and 624 kg for NI, REV-S, and REV-X, respectively), ADG (1.25, 1.51, and 1.60 kg), and G:F (0.14, 0.16, and 0.17) increased (P < 0.05) as TBA and E(2) dose increased. Implanting increased (P < 0.05) DMI, but DMI did not differ (P > 0.10) between REV-S and REV-X (8.8 for NI vs. 9.4 kg/d for the 2 implants). From d 1 to 112 of the feeding period, implanting increased (P < 0.05) ADG and G:F, but REV-S and REV-X did not differ (P > 0.10). From d 112 to end, ADG increased by 19% (P < 0.05) and G:F was 18% greater (P < 0.05) for REV-X vs. REV-S. Carcass-adjusted final BW (29-kg difference), ADG (0.2-kg/d difference), and G:F (0.02 difference) were increased (P < 0.05) by ZH, but daily DMI was not affected by feeding ZH. Hot carcass weight was increased (P < 0.05) by ZH (19-kg difference) and implant, with REV-X resulting in the greatest response (HCW of 376 for NI vs. 404 and 419 kg for REV-S and REV-X, respectively; P < 0.05). An implant × ZH interaction (P = 0.05) occurred for dressing percent (DP). Without ZH, implanting increased DP, but DP did not differ (P > 0.10) between REV-X and REV-S. With ZH, REV-X increased (1.7%; P < 0.05) DP vs. NI and REV-S. Marbling score, 12th-rib fat, and KPH were not affected (P > 0.10) by implant or ZH. Overall, treatment increased steer performance and HCW in an additive fashion, suggesting different mechanisms of action for ZH and steroidal implants. In addition, a greater dose of TBA + E(2) and extended payout improved steer performance and HCW.

Effects of roughage concentration in steam-flaked corn-based diets containing wet distillers grains with solubles on feedlot cattle performance, carcass characteristics, and in vitro fermentation.

Two studies were conducted to evaluate effects of wet distillers grains with solubles (WDG) and dietary concentration of alfalfa hay (AH) on performance of finishing beef cattle and in vitro fermentation. In both studies, 7 treatments were arranged in a 2 × 3 + 1 factorial; factors were dietary concentrations (DM basis) of WDG (15 or 30%) and AH (7.5, 10, or 12.5%) plus a non-WDG control diet that contained 10% AH. In Exp. 1, 224 beef steers were used in a randomized complete block (initial BW 342 kg ± 9.03) finishing trial. No WDG × AH interactions were observed (P > 0.12). There were no differences among treatments in final shrunk BW or ADG (P > 0.15), and DMI did not differ with WDG concentration for the overall feeding period (P = 0.38). Increasing dietary AH concentration tended (P < 0.079) to linearly increase DMI, and linearly decreased (P < 0.05) G:F and calculated dietary NE(m) and NE(g) concentrations. Carcasses from cattle fed 15% WDG had greater yield grades (P = 0.014), with tendencies for greater 12th-rib fat (P = 0.054) and marbling score (P = 0.053) than those from cattle fed 30% WDG. There were no differences among treatments (P > 0.15) in HCW, dressing percent, LM area, KPH, proportions of cattle grading USDA Choice, and incidence of liver abscesses. In Exp. 2, ruminal fluid was collected from 2 ruminally cannulated Jersey steers adapted to a 60% concentrate diet to evaluate in vitro gas production kinetics, H(2)S production, IVDMD, and VFA. Relative to the control substrate, including WDG in substrates increased (P < 0.01) H(2)S production and decreased total gas production (P = 0.01) and rate of gas production (P = 0.03). Increasing substrate WDG from 15 to 30% increased (P < 0.05) H(2)S production and decreased (P < 0.001) total gas production, with a tendency (P = 0.073) to decrease IVDMD and fractional rate of gas production (P = 0.063). Treatments did not significantly affect (P > 0.09) molar proportions or total concentration of VFA. Results indicate that including 15 or 30% WDG in steam-flaked corn-based diets did not result in major changes in feedlot performance or carcass characteristics, but increasing AH concentration from 7.5 to 12.5% in diets containing WDG decreased G:F. Including WDG in substrates decreased rate and extent of gas production and increased H(2)S production. Changes in various measures of in vitro fermentation associated with AH concentrations were not large.

Effects of high-oil corn on feedlot performance, carcass characteristics, fatty acid profiles, beef palatability, and retail case life traits of beef top loin steaks.

Our objective was to compare the effects of feeding steam-flaked, high-oil corn with normal steam-flaked corn to which yellow grease was added to equalize dietary fat on performance and carcass characteristics of finishing beef steers, and palatability, retail case life, and fatty acid composition of strip loins. Angus steers (n = 120; initial BW = 288 kg) were allotted to dietary treatments consisting of 1) normal mill-run, steam-flaked corn plus added fat (NMR) or 2) high-oil, steam-flaked corn (HOC) and assigned randomly to pens (12 pens/treatment with 5 steers/pen). Performance (ADG, DMI, and G:F) was measured over time, and cattle were shipped to a commercial abattoir for collection of carcass data after 165 d on feed. Carcass data were collected at 48 h postmortem on all carcasses, and 2 carcasses from each pen were selected randomly for collection of strip loins (IMPS #180A). At 14 d postmortem, 4 steaks (2.54 cm thick) were removed for retail display, trained sensory panel analysis, Warner-Bratzler shear force determination, and fatty acid analysis. Daily BW gain was greater (P = 0.03) and G:F was increased 8.4% (P = 0.01) for steers fed NMR compared with HOC, but DMI was not affected (P > 0.10) by treatment. No treatment differences were observed (P > 0.10) for HCW, 12th-rib fat, KPH, and yield grade. Marbling scores were greater (P = 0.01) for NMR than for HOC, and LM area tended (P = 0.07) to be greater in NMR than in HOC carcasses. The proportion of carcasses grading USDA Choice did not differ (P = 0.77) between treatments, but a greater (P = 0.04) proportion of carcasses graded in the upper two-thirds of Choice for NMR vs. HOC. Trained sensory panel traits and Warner-Bratzler shear force values did not differ between treatments (P > 0.10), and no differences (P > 0.10) were detected for purge loss or fatty acid composition. Overall, ADG and G:F were less and marbling score was decreased, but there were no differences between treatments in beef palatability, retail case life, or concentrations of fatty acids in strip loins.

Dose and release pattern of anabolic implants affects growth of finishing beef steers across days on feed.

Four experiments evaluated the effect of implant dose and release pattern on performance and carcass traits of crossbred beef steers. In Exp. 1, steers (4 to 7 pens/treatment; initial BW = 315 kg) were fed an average of 174 d. Treatments were 1) no implant (NI); 2) Revalor-S [120 mg of trenbolone acetate (TBA) and 24 mg of estradiol 17ß (E(2)); REV-S]; 3) Revalor-IS followed by REV-S (cumulatively 200 mg of TBA and 40 mg of E(2); reimplanted at 68 to 74 d; REV-IS/S); and 4) Revalor-XS (200 mg of TBA and 40 mg of E(2); REV-X). Carcass-adjusted final BW was greater (P < 0.05) for REV-X and REV-IS/S than for REV-S (610, 609, and 598 kg, respectively). Daily DMI did not differ (P > 0.10) among the 3 implants, but carcass-adjusted G:F was greater (P < 0.05) for REV-X and REV-IS/S than for REV-S (0.197 and 0.195 vs. 0.188). Both HCW and LM area were greater (P < 0.05) for REV-X and REV-IS/S than for REV-S. Marbling scores were greatest (P < 0.05) for REV-S and least (P < 0.05) for REV-IS/S; REV-X was intermediate to NI and REV-IS/S. In Exp. 2, steers (10 pens/treatment; initial BW = 391 kg) were fed 131 d, with treatments of REV-S, REV-IS/S (reimplanted at 44 to 47 d), and REV-X. Carcass-adjusted final BW (598 kg), ADG (1.6 kg), DMI (9.4 kg), G:F (0.17), and HCW did not differ (P > 0.10) among treatments. The percentage of Choice was less (P < 0.05) and percentage of Select greater (P < 0.05) for REV-IS/S than for REV-S and REV-X. In Exp. 3, steers (10 pens/treatment; initial BW = 277 kg) were fed 197 d and received either REV-IS/S (reimplanted at 90 to 103 d) or REV-X. Carcass-adjusted final BW (625 vs. 633 kg) and ADG (1.81 vs. 1.76 kg) were greater (P < 0.05) for REV-X-implanted steers. Daily DMI did not differ, but G:F tended (P < 0.10) to be increased and HCW was greater (P < 0.05) for REV-X than for REV-IS/S. In Exp. 4, steers (8 pens/treatment; initial BW = 238 kg) were fed 243 d and received either REV-IS/S (reimplanted at 68 to 71 d) or REV-X. Carcass-adjusted final BW (612 kg), ADG (1.54 kg), DMI (7.55), and G:F (0.21) did not differ (P > 0.10) for REV-IS/S and REV-X-implanted steers. Carcass traits did not differ among implants, but the percentage of Choice carcasses was greater (P < 0.05) and percentage of Select was less (P < 0.05) for REV-X than for REV-IS/S. These data indicate that when TBA/E(2) dose is equal, the altered release rate of REV-X can improve performance and quality grade, but these effects depend on duration of the feeding period and timing of initial and terminal implants.

Alpharma Beef Cattle Nutrition Symposium: predictability of feedlot cattle growth performance.

Predicting performance is vital to management and marketing decisions in commercial feedlots. Agreement between performance predicted from NE equations or empirical regression relationships and actual performance is generally very good, suggesting that factors affecting performance by finishing cattle are fairly well documented. The challenge for feedlot managers is to predict performance with limited information at the start of the feeding period. Data on sex and initial shrunk BW (ISBW) are typically available when cattle start on feed. Relationships between ISBW, sex, and performance were evaluated using 3,363 pen records collected over 4 yr from 3 commercial feedlots in the Texas Panhandle. Mixed-model regression was used to account for random effects of feedlot × season × year and fixed effects of ISBW (range = 227 to 451 kg), sex (steer or heifer), and ISBW × sex (P < 0.10 for all variables evaluated). Previously developed equations indicated that with intercept and slope adjustments for sex, ISBW accounted for 76 and 84% of the variation in DMI and final shrunk BW (FSBW), respectively. Similarly, newly developed regression equations that included ISBW, sex, and ISBW × sex accounted for 46 and 81% of the variation in ADG and HCW, respectively. Initial BW was negatively related to G:F (R(2) = 0.22). Including early DMI data (DMI from d 8 to 28) increased R(2) and decreased prediction error for DMI, indicating that updating predictions with interim intake data might prove beneficial. An independent data set (781 lots of steers and heifers) collected during 1 yr from 2 Texas Panhandle feedlots was used to validate equations developed with the larger database. Dry matter intake predicted from ISBW and sex accounted for 69% of the variation in observed DMI (SE of prediction = 0.47; mean bias = 0.42 kg). Predicting DMI with ISBW, sex, and DMI from d 8 to 28 of the feeding period increased r(2) to 0.76 and slightly decreased the SE of prediction (0.42 kg), but the equation had a strong linear bias (-0.174; P < 0.001). The r(2) values for regression of observed on predicted ADG, G:F, FSBW, and HCW were 0.37, 0.08, 0.74, and 0.73, respectively, with positive mean bias (underprediction for all equations). Average daily gain calculated with NE equations from predicted DMI (ISBW and sex equation) and predicted FSBW had a similar r(2) (0.38) but less mean bias (-0.08 kg) than ADG predicted directly from ISBW and sex. Adjustments to equations for animal type, health, and management effects would likely improve predictions. Nonetheless, results suggest that predicting performance from initial BW with adjustments for steers vs. heifers should have considerable utility in practical settings.

Effects of ractopamine and trenbolone acetate implants with or without estradiol on growth performance, carcass characteristics, adipogenic enzyme activity, and blood metabolites in feedlot steers and heifers.

Two experiments were conducted to evaluate effects of ractopamine (RAC) and steroidal implant treatments on performance, carcass traits, blood metabolites, and lipogenic enzyme activity in feedlot cattle. In Exp. 1, yearling steers (n = 486; initial BW = 305 kg) were used in a 3 × 3 factorial arrangement of RAC doses of 0 (R0), 100 (R100), or 200 (R200) mg·steer(-1)·d(-1) fed for 28 d and implant regimens (implant-reimplant) of no implant-no reimplant (NI-NI), 120 mg of trenbolone acetate (TBA) and 24 mg of estradiol-17ß (E17B)-no implant (RS-NI), or 80 mg of TBA and 16 mg of E17B followed by 120 mg of TBA and 24 mg of E17B (RI-RS). Except for KPH and skeletal maturity score, no RAC × implant interactions were noted (P > 0.10). Carcasses from R200 were 6.3 kg (P = 0.042) heavier than those from R0. Marbling, calculated empty body fat (EBF), and USDA quality grade did not differ (P > 0.10) among RAC treatments. The RI-RS steers had 12.6 kg (P = 0.001) and 41.1 kg (P < 0.001) greater HCW than RS-NI and NI-NI, respectively. Despite no difference (P > 0.10) in EBF, marbling score was decreased for RI-RS (P < 0.001) and RS-NI (P = 0.001) relative to NI-NI, resulting in 14.6 and 11.4 percentage unit fewer USDA Prime and Choice carcasses with RI-RS (P = 0.008) and RS-NI (P = 0.039) than with NI-NI. In Exp. 2, heifers (n = 48; initial BW = 347 kg) were used in a 3 × 2 factorial arrangement of RAC doses of 0 (R0) or 250 (R250) mg·heifer(-1)·d(-1) and implant regimens of none (NI), 200 mg of TBA (TO), or 200 mg of TBA and 20 mg of E17B (TE). Blood samples were collected at various times during the feeding period, and subcutaneous adipose samples were collected on d 119. For growth and carcass measurements, no RAC × implant interactions (P > 0.10) were detected. The RAC-supplemented heifers had greater HCW (P < 0.10) with no difference in marbling score. For implant regimens, TE heifers had greater HCW than the NI (P = 0.001) and TO (P = 0.037) heifers. Although EBF did not differ among implant treatments (P > 0.10), TE (P = 0.021) and TO (P = 0.039) had fewer Choice carcasses than NI. Heifers with implants had decreased cortisol and increased IGF-1 and NEFA (P < 0.10) compared with NI heifers. An implant × RAC interaction was detected (P = 0.001) for serum urea nitrogen (SUN), with TE and RAC-supplemented heifers having decreased SUN. These data suggest that the effects of implant and RAC on growth and carcass traits are independent and that USDA quality grade and marbling score can differ significantly among carcasses with similar calculated EBF values.