RESUMO
The anticancer activity of eight crude extracts of Smilax china L. rhizome (SCR) against HeLa cells was assessed by MTT assay and clonogenic assay, the fraction rich in flavonoids had show good activity against HeLa cells. A bioassay-guided separation on this extract lead to the detection of kaempferol-7-O-beta-D-glucoside (KG), which belongs to flavonoid glycoside, displayed marked anticancer activity. We evaluated its in vitro cytotoxicity and antiproliferative effect in a panel of established cancer cell lines by MTT assay and clonogenic assay. KG induces A375 and HL60 cells apoptosis, which was demonstrated by morphological changes, DNA fragmentation and flow cytometric analysis. Fluorescent staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the A375 and HL60 cells. Flow cytometric analysis shown that A375 and HL60 cells treated with KG resulted in the appearance of a hypodiploid peak (A0 region), probably due to the presence of apoptosing cells and/or apoptotic bodies with DNA content less than 2n. Quantitation of the hypodiploid cells shows a dose-dependent response to KG, and this result is in good accordance with that of the DNA fragmentation assay by agarose gel electrophoresis. Our results suggested that cell cycle arrest at G(1) phase and induce apoptosis as a mechanism by which KG exerts an antiproliferative effect.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Flavonoides/farmacologia , Glucosídeos/farmacologia , Quempferóis/farmacologia , Smilax/química , Antineoplásicos Fitogênicos/administração & dosagem , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Fragmentação do DNA , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Eletroforese em Gel de Ágar , Flavonoides/administração & dosagem , Citometria de Fluxo , Fase G1/efeitos dos fármacos , Glucosídeos/administração & dosagem , Glicosídeos/administração & dosagem , Glicosídeos/farmacologia , Células HeLa , Humanos , Quempferóis/administração & dosagem , Pinellia , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologiaRESUMO
OBJECTIVE: To compare the content of the active ingredient resveratrol in Smilax china from different habitats. METHOD: The ingredients of samples from different habitats in China were analyzed for resveratrol in S. china by HPLC. RESULT: There was a significant differences in resveratrol content between the samples. CONCLUSION: Resveratrol content in the sample from Qianshan (Anhui province) is obvious higher than those from other habitats.