An ayurvedic perspective along with in silico study of the drugs for the management of SARS-CoV-2.

BACKGROUND: COVID-19 is the disease caused by SARS-CoV2, it was identified in Wuhan, China, in 2019. It then extended across the globe and was termed as a pandemic in 2020. Though research work on its vaccine and drugs are carried out across the globe, it is even necessary to look over it through alternative sciences. OBJECTIVE: The objective of this study is to look over the disease through Ayurvedic perspective, analyse possible pathologies, select appropriate drugs and to study in-silico screening on these selected drugs. MATERIALS & METHODS: Available symptoms of COVID-19 were thoroughly studied and reviewed through Ayurveda classics, internet, preprints, etc. to understand the nature of the disease with the Ayurvedic perspective. The molecular Docking and Grid were generated through Pyrx Software with Autodock. The Lipinski Rule of Five data generated from Swiss ADME software and Target prediction of selected phytoconstituents were done by Swiss target prediction. RESULTS: In Ayurveda, COVID-19 can be considered as Janapadaudhwans, Va t a -Kaphaj a Sannipatik a Jwara, Aup a sargika Vyadhi, and Dhatupaka Awastha. In the molecular docking study, the binding energy and inhibition of 6 Gingesulphonic acid from Zingiber officinalis (Sunthi) is greater than hydroxychloroquine and quinine. Most of the selected phytoconstituents follow the Lipinski rule of five. Target prediction of selected phytoconstituents was done on target of SARS-CoV-2, humoral immunity, and antiviral activity. Every selected phytoconstituents works on minimum one of the targets. CONCLUSION: Thus, from the above results obtained from reviewing Ayurveda classics and after the virtual screening of selected drugs we can conclude that Nagara di Kashaya (Sunthi, Puskarmoola, Kantakari, Guduchi) may have appreciable results in combating SARS-CoV-2. Thus, Nagara di Kashayam, a classical formulation can be a trial candidate for conducting further clinical trial.

Management of Vandhyatva w.s.r. to thin endometrium through Ayurveda- A single arm open labelled pilot clinical trial.

Background: The four essential factors of conception are Ritu (optimum period for conception), Kshetra (place of conception), Ambu (nutrition), and Beeja (healthy sperm and ovum). Kshetra denotes body as general and to be very specific, especially about the female reproductive system, it is Garbhashaya or the uterine cavity and function is Garbhadhana or conception. The endometrial factor is an important subset in infertility due to poor implantation. Aims: To evaluate the efficacy of Saubhagyanandana Ghrita Yonipichu (vaginal tampoon) and Jeevaniya Churna orally in the management of thin endometrium thickness and to evaluate its efficacy in the associate complains of menstrual abnormalities, i.e., irregularity of menstrual cycle, quantity, duration and pain. Materials and methods: It was a single-arm open labeled pilot clinical trial, a total of 15 female patients having endometrial thickness <7 mm on transvaginal sonography findings were included in the study. Each patient (n = 15) was given Saubhagyanandana Ghrita Yonipichu (10 mL for 6 days after cessation of menses, for 1 cycle) along with Jeevaniya Churna orally 10 g along with milk twice before meals for 30 days. Assessment of the study was done on Appelbaum's uterine scoring system for the reproduction (USSR) scoring pattern. Results: Results were analyzed by the paired' t-test for the objective parameters and Wilcoxon signed rank-sum test for the subjective parameters. A statistically highly significant difference (P < 0.001) was seen in endometrium thickness, layering, myometrial echogenicity, uterine Doppler flow, endometrial blood flow in zone 3, and total USSR score. Statistical significant difference (P < 0.05) was found in the menstrual abnormalities and a highly statistically significant difference (P < 0.001) was found in ovulation. One of the patients conceived after the treatment. Conclusion: Saubhagyanandana Ghrita Yonipichu (vaginal tampoon) and Jeevaniya Churna orally are effective in improving the thin endometrium.

Establishment of quality function deployment with special reference to good manufacturing practice of Chandraprabha Vati.

CONTEXT: Quality function deployment (QFD) is a powerful tool for the translation of vague, imprecise customer requirements into clear, measurable technical requirements. AIMS: The objective of this article is twofold: (a) to propose a platform conceptual model for Chandraprabha Vati and (b) to present 10-step procedure to build QFD matrix. MATERIALS AND METHODS: The method deals with, first, preparing two conceptual models for Chandraprabha Vati for the sake of manufacturing unit. The survey study was carried out for two products for the selection of customer requirement. Then, with the help of survey study QFD's house of quality matrix was prepared which contained two products, i.e., classical product prepared by pharmacy of university and marketed product. High performance thin layer chromatography study was also carried out. STATISTICAL ANALYSIS USED: Principal component analysis (PCA) was applied to the data using unscrambler software. RESULTS: The results of QFD chart and PCA analysis were obtained from the two surveys. The first survey reveals that if proper care is taken during preparation on hardness and capping of Vati, then product goes toward zero defects. The second survey reveals that if proper care is taken during preparation on hardness, capping and sticking, then physically Vati goes toward zero defects. CONCLUSIONS: The 10-step procedure facilitates the process of reasoning and reduces the time spent for manufacturing with zero defect in product.