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1.
J Vet Diagn Invest ; 26(6): 795-8, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25292192

RESUMO

Microbiological and histological analysis of a sample from a swollen testicle of a 2-year-old Border Collie dog revealed a mixed infection of the fungus Blastomyces dermatitidis and the Gram-negative bacterium Aureimonas altamirensis. When subjected to an automated microbial identification system, the latter isolate was provisionally identified as Psychrobacter phenylpyruvicus, but the organism shared several biochemical features with Brucella canis and exhibited agglutination, albeit weakly, with anti-B. canis antiserum. Unequivocal identification of the organism was only achieved by 16S ribosomal RNA gene sequencing, ultimately establishing the identity as A. altamirensis. Since its first description in 2006, this organism has been isolated infrequently from human clinical samples, but, to the authors' knowledge, has not been reported from a veterinary clinical sample. While of unknown clinical significance with respect to the pathology observed for the polymicrobial infection described herein, it highlights the critical importance to unambiguously identify the microbe for diagnostic, epidemiological, infection control, and public health purposes.


Assuntos
Alphaproteobacteria/isolamento & purificação , Doenças do Cão/diagnóstico , Doenças do Cão/microbiologia , Edema/veterinária , Infecções por Bactérias Gram-Negativas/veterinária , Doenças Testiculares/veterinária , Alphaproteobacteria/genética , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , DNA Bacteriano/genética , DNA Bacteriano/metabolismo , Cães , Edema/diagnóstico , Edema/microbiologia , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/microbiologia , Masculino , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , Análise de Sequência de DNA/veterinária , Doenças Testiculares/diagnóstico , Doenças Testiculares/microbiologia , Resultado do Tratamento
2.
Appl Environ Microbiol ; 75(11): 3745-54, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19363079

RESUMO

Clostridium perfringens is a gram-positive anaerobe and a pathogen of medical importance. The detection of acid phosphatase activity is a powerful diagnostic indicator of the presence of C. perfringens among anaerobic isolates; however, characterization of the enzyme has not previously been reported. Provided here are details of the characterization of a soluble recombinant form of this cell-associated enzyme. The denatured enzyme was approximately 31 kDa and a homodimer in solution. It catalyzed the hydrolysis of several substrates, including para-nitrophenyl phosphate, 4-methylumbelliferyl phosphate, and 3' and 5' nucleoside monophosphates at pH 6. Calculated K(m)s ranged from 0.2 to 0.6 mM with maximum velocity ranging from 0.8 to 1.6 micromol of P(i)/s/mg. Activity was enhanced in the presence of some divalent cations but diminished in the presence of others. Wild-type enzyme was detected in all clinical C. perfringens isolates tested and found to be cell associated. The described enzyme belongs to nonspecific acid phosphatase class C but is devoid of lipid modification commonly attributed to this class.


Assuntos
Fosfatase Ácida/metabolismo , Proteínas de Bactérias/metabolismo , Clostridium perfringens/enzimologia , Fosfatase Ácida/química , Proteínas de Bactérias/química , Cátions Bivalentes/farmacologia , Dimerização , Ativadores de Enzimas/farmacologia , Inibidores Enzimáticos/farmacologia , Himecromona/análogos & derivados , Cinética , Peso Molecular , Nitrofenóis/metabolismo , Nucleosídeos , Compostos Organofosforados/metabolismo , Especificidade por Substrato
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