RESUMO
At least 59 DRB1*14 positive individuals from each of four U.S. population groups, Caucasoids, African Americans, Asians/Pacific Islanders, and Hispanics, were randomly selected from a database of 82,979 individuals. DRB1*14 alleles were identified by DNA sequence analysis using intron-specific primers to obtain complete exon 2 sequences. Only 23% of the known DRB1*14 alleles were detected. DRB1*14011 was the predominant DRB1*14 allele in three populations while Hispanics carried DRB1*1402 and DRB1*1406 more frequently. Asians/Pacific Islanders were the most diversified carrying seven alleles. DRB3*0101, DRB3*02021 and DRB3*0210 were detected in a subset of individuals typed for this locus and 15 DRB1-DRB3 haplotypes were defined. This study completes the exon 2 sequences of previously identified alleles, DRB1*1405-*1408, including the identification of two silent codon 90 variants of DRB1*1407. In addition, two new DRB1*14 alleles, DRB1*1441 and DRB1*1442, are described.
Assuntos
Variação Genética , Antígenos HLA-DR/genética , Povo Asiático/genética , População Negra/genética , Éxons , Frequência do Gene , Genética Populacional , Cadeias HLA-DRB1 , Cadeias HLA-DRB3 , Haplótipos , Hispânico ou Latino/genética , Humanos , Estados Unidos , População Branca/genéticaRESUMO
This paper describes 10 novel HLA-A alleles that have been characterized by DNA sequencing. Seven alleles, A*0308, A*2616, A*3009, A*3206, A*3403, A*3602 and A*6604 carry motifs observed in other HLA-A alleles, suggesting that gene conversion has created this diversity. The remaining three alleles, A*01012, A*0306 and A*2617, contain polymorphisms not previously found in any "classical" class I allele. All alleles were identified due to unexpected probe hybridization patterns during routine SSOP typing. Exons 2 and 3 of each allele were subsequently characterized by DNA sequencing.
Assuntos
Alelos , Antígenos HLA-A/genética , Humanos , Polimorfismo GenéticoRESUMO
This paper describes seven novel HLA-B alleles. Five of these new alleles contain polymorphic motifs previously reported in HLA-B alleles, suggesting an origin resultant from a gene conversion mechanism. B*0723 contains a polymorphism previously unreported in class I HLA molecules. B*4105 contains a nucleotide substitution previously unreported in class I HLA molecules, which encodes a protein sequence previously reported only in HLA-C locus alleles.
Assuntos
Alelos , Antígenos HLA-B/genética , Células Cultivadas , Antígenos HLA-B/imunologia , Humanos , Dados de Sequência Molecular , Família Multigênica , Homologia de SequênciaRESUMO
This report describes eight novel HLA-A alleles. Seven of these new alleles contain substitutions previously reported in other HLA-A alleles, suggesting an origin resulting from a gene conversion mechanism. Of these seven, A*2431 contains a substitution previously associated with a larger polymorphic motif. A*0247 contains a substitution at a previously polymorphic position, but encodes an amino acid change previously unreported in any HLA-A, -B or -C alleles.
Assuntos
Alelos , Antígenos HLA-A/genética , Sequência de Bases , Reações Cruzadas , Éxons , Antígenos HLA-A/análise , Antígenos HLA-A/imunologia , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Recombinação Genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
Five of the six novel HLA-B alleles described in this report contain polymorphic motifs previously reported in HLA-B alleles, suggesting an origin resultant from a gene conversion mechanism. The sixth allele, B*35012, contains a polymorphism previously unreported in HLA-B molecules. In addition to previously reported polymorphisms, B*5806 carries a previously unreported intronic substitution in a sequencing primer annealing site.
Assuntos
Alelos , Antígenos HLA-B/genética , Íntrons , Sequência de Bases , Reações Cruzadas , Antígenos HLA-B/imunologia , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo Genético , Recombinação Genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
Twelve new B*15 alleles are described. All of the known B*15 alleles are divided into subgroups based on serologic assignments and/or nucleotide sequence polymorphisms. These groups might be used as a reference for DNA-based testing at an intermediate (i.e. "serologic") level of resolution.
Assuntos
Alelos , Antígenos HLA-B/genética , Motivos de Aminoácidos , Sequência de Aminoácidos , Éxons/imunologia , Conversão Gênica/imunologia , Antígenos HLA-B/química , Antígeno HLA-B15 , Humanos , Dados de Sequência Molecular , Família Multigênica/imunologiaRESUMO
This paper describes 29 novel HLA-B locus alleles identified during low-resolution typing. The majority of the novel alleles carry new patterns of previously known polymorphic motifs or codons. Three alleles carry alterations in the Bw4/Bw6 epitope. Five alleles carry novel substitutions.
Assuntos
Alelos , Antígenos HLA-B/genética , Família Multigênica/imunologia , Reações Cruzadas/genética , Antígenos HLA-B/imunologia , Humanos , Dados de Sequência MolecularRESUMO
This paper describes 9 novel HLA-B locus alleles. All of the alleles carry sequence motifs observed in other HLA-B alleles.
Assuntos
Alelos , Antígenos HLA-B/genética , Família Multigênica/imunologia , Antígenos HLA-B/imunologia , Humanos , Dados de Sequência MolecularRESUMO
This paper describes 13 novel HLA-B locus alleles, B*0809, B*0812, B*0813, B*0814, B*14062, B*3804, B*3806, B*3914, B*3915, B*3918, B*3919, B*3920, and B*3922 which represent new patterns of known polymorphic residues.
Assuntos
Alelos , Antígenos HLA-B/genética , Proteínas Repressoras/genética , Antígeno HLA-B14 , Antígeno HLA-B18 , Antígeno HLA-B38 , Antígeno HLA-B39 , HumanosRESUMO
Seven new HLA-B locus alleles have been described. B*44022 and B*44032 are silent substitutions altering known alleles. B*4411 carries a unique Bw4-like epitope. B*4420, B*4421, and B*4424 carry new combinations of motifs previously observed in other alleles. B*8301 appears to be the result of the replacement of exon 2 from B*4402 with exon 2 from B*5603.