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The objective of this work was to assess the efficacy of different proteolytic agents on the bond strength of pit and fissure sealants to bovine enamel. Eighty-four bovine enamel specimens were randomly assigned in groups according to the pit and fissure sealant applied (HelioSeal F or Dyad Flow). Then, the specimens were subdivided according to the proteolytic agent used (n = 7): Group 1, distilled water (control); Group 2, 10 wt.% Tergazyme®; Group 3, 10 wt.% ZYME®; Group 4, 10% papain gel; Group 5, 10% bromelain gel; and Group 6, 5.25 wt.% sodium hypochlorite. The cell viability of the proteolytic solutions was assessed through the MTT assay. The proteolytic agents were applied on the enamel surface prior to the acid-etching procedure; then, the pit and fissure sealants were placed. The micro-shear bond strength was evaluated after 24 h or 6 months of water storing at 37 °C. Representative SEM images were taken for each experimental group. The bond strength data were statistically analyzed by a three-way ANOVA test using a significance level of α = 0.05. Bromelain and papain proteolytic solutions did not exert any cytotoxic effect on the human dental pulp cells. After 24 h and 6 months of aging, for both pit and fissure sealants, sodium hypochlorite, papain, bromelain, and Tergazyme® achieved statistically significant higher bond strength values (p < 0.05). Irrespective of the deproteinizing agent used, Dyad Flow resulted in a better bond strength after 6 months of aging. The type 1 etching pattern was identified for sodium hypochlorite, papain, and bromelain. Tergazyme®, papain, and bromelain demonstrated efficacy in deproteinizing enamel surfaces prior to acid etching, leading to the improved bond strength of pit and fissure sealants. Clinically, this suggests that these proteolytic agents can be considered viable alternatives to traditional methods for enhancing sealant retention and longevity. Utilizing these agents in dental practice could potentially reduce sealant failures.
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Chitosan, a natural polysaccharide sourced from crustaceans and insects, is often used with hydrogels in wound care. Evaluating its cytotoxicity and antimicrobial properties is crucial for its potential use in dentistry. OBJECTIVE: To investigate the mechanical properties of gelatin hydrogels based on decaethylated chitosan and antimicrobial activity against Streptococcus mutans and their biological effects with stem cells from apical papilla (SCAPs). MATERIAL AND METHODS: Gelatin-chitosan hydrogels were synthesized at concentrations of 0%, 0.2% and 0.5%. Enzymatic and hydrolytic degradation, along with swelling capacity, was assessed. Fourier transform infrared spectroscopy (FTIR) analysis was employed to characterize the hydrogels. The interaction between hydrogels and SCAPs was examined through initial adhesion and cell proliferation at 24 and 48 h, using the Thiazolyl Blue Tetrazolium Bromide (MTT assay). The antimicrobial effect was evaluated using agar diffusion and a microdilution test against S. mutans. Uniaxial tensile strength (UTS) was also measured to assess the mechanical properties of the hydrogels. RESULTS: The hydrogels underwent hydrolytic and enzymatic degradation at 30, 220, 300 min and 15, 25, 30 min, respectively. Significantly, (p < 0.01) swelling capacity occurred at 20, 40, 30 min, respectively. Gelatin-chitosan hydrogels' functional groups were confirmed using vibrational pattern analysis. SCAPs proliferation corresponded to 24 h = 73 ± 2%, 82 ± 2%, 61 ± 6% and 48 h = 83 ± 11%, 86 ± 2%, 44 ± 2%, respectively. The bacterial survival of hydrogel interaction was found to be 96 ± 1%, 17 ± 1.5% (p < 0.01) and 1 ± 0.5% (p < 0.01), respectively. UTS showed enhanced (p < 0.05) mechanical properties with chitosan presence. CONCLUSION: Gelatin-chitosan hydrogels displayed favorable degradation, swelling capacity, mild dose-dependent cytotoxicity, significant proliferation with stem cells from apical papilla (SCAPs), substantial antimicrobial effects against S. mutans and enhanced mechanical properties. These findings highlight their potential applications as postoperative care dressings.
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ABSTRACT: The objective of this study was to determinate the fracture resistance of endodontically treated teeth and restored with two root post systems: i) resin post, ii) fiber post. A total of 60 teeth were freshly extracted, endodontic ally treated and randomly divided in two groups (n= 30/each group) for standardized restoration; Group 1 (Group R): Resin post and resin restoration, Group 2 (Group FP): Fiberglass post and resin restoration. Both groups' samples were mounted in a metallic base at 135º to allow them to be stabilized and held in the universal testing machine by applying a vertical force at cross speed of 1mm/min. Data were recorded in Newtons (N) Previous to test the fracture resistance; all samples were stored in distilled water at 37 ºC for 24 hours. Data were subject to the Saphiro-Wilk test for normality distribution and Student's t test. Significance was considered at 0.05 values. The values of fiber post group showed normal distribution compared to the resin group, demonstrating less variability among the values. The group FP displayed higher fracture resistance (299.77±100 N) than group R (205.57±86.40 N), with significant differences (p= 0.00002). The greatest fracture resistance was recorded for the group having fiber post reinforced and composite cores. It is suggested that fiberglass post restoration is the first option when endodontic treatment requires core restoration.
RESUMEN: El objetivo del estudio fue determinar la resistencia a la fractura de dientes tratados endodónticamente y restaurados con dos sistemas de endopostes radiculares: I) poste de resina, II) poste de fibra. Un total de 60 dientes recién extraídos fueron tratados endodónticamente y divididos al azar en dos grupos (n= 30/ cada grupo) para la restauración estandarizada; Grupo 1 (Grupo R): Pilares de resina y restauración, Grupo 2 (Grupo FP): Pilares de fibra de vidrio y restauración de resina. Las muestras de ambos grupos se montaron en una base metálica a 135º para permitir su estabilización y sujeción en la máquina universal de ensayos aplicando una fuerza vertical a velocidad transversal de 1 mm/min. Los datos se registraron en Newtons (N), para probar la resistencia a la fractura; todas las muestras se almacenaron en agua destilada a 37 ºC durante 24 horas. Los datos se sometieron a la prueba de normalidad de Saphiro-Wilk y la pruebas t de Student. La significancia se consideró con un valor de 0,05. Los valores del grupo de postes de fibra mostraron una distribución normal en comparación con el grupo de resinas, demostrando menor variabilidad entre los valores. El grupo FP mostró mayor resistencia a la fractura (299,77±100 N) que el grupo R (205,57±86,40 N) que el grupo con diferencias significativas (p= 0,00002). La mayor resistencia a la fractura se registró para el grupo que tenía núcleos compuestos y reforzados con postes de fibra. Se sugiere que la restauración posterior de fibra de vidrio es la primera opción cuando el tratamiento de endodoncia requiere una restauración del núcleo.
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Objective: To perform a literature review on oral squamous cell carcinoma, the presence of cancer stem cells; their association with the course of the disease and therapeutic applications. Methods: : A search was performed in the PubMed database by entering the following algorithm: ((((neoplastic stem cells [MeSH Terms ]) OR (Cancer stem cells [Text Word ])) AND (Squamous Cell Carcinoma of Head and Neck [MeSH Terms])) AND (Oral squamous cell carcinoma [Text Word ]), to find articles in english published between 2012 and 2022. The PRISMA diagram was used to identify and select the articles. Results: A result of 49 articles was obtained; of which 27 were chosen according to the title and abstract in their association with the topic. In addition, 8 additional articles suggested by their relationship with the information previously searched were included. In total, 35 articles were evaluated. There has been found that tumoral cells in squamous oral carcinoma are heterogeneous since they include cancer stem cells wich possess characteristics of stem and neoplasic cells; which possess characteristics of stem cells as well as neoplastic cells; they have been associated with disease progression, recurrence, and metastasis and have been considered to be a key mechanism of therapy failure. Conclusions: The expression of stem cell markers in oral squamous cell carcinomas has been demonstrated and has contributed to their identification in oral squamous cell carcinomas and has been implicated in the behavior of cancer cells. New therapeutic measures aimed at eliminating cancer stem cells have been proposed and developed.
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ABSTRACT Chitosan is a biopolymer with bactericidal/bacteriostatic effect, biocompatible and biodegradable. It has been used in tissue engineering to replace tissues partially or completely by releasing bioactive materials or influencing cell growth, usually in regenerative medicine and dentistry. The aim of this study was to evaluate the cytotoxic and anti-inflammatory effect of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF were isolated from patients. Cells were subcultured in DMEM. Chitosan was inoculated at different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) were placed directly in the presence of cells and incubated for 24 hours. Cell viability was determined by MTT method and mean cytotoxic concentration (CC50) was calculated from the dose-response curve. Anti-inflammatory effect was calculated from the in vitro gingivitis model induced with interleukin 1beta (IL-1β) in HGF and protein detection. The data were subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney tests. Experiments were performed in triplicate of three independent assays. Cell viability of HPC, HGF and MC3T3-E1 in contact with chitosan decreased significantly (p<0.05). The HPC were the most sensitive (CC50= 0.18%), followed by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%). The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory effect was reduced significantly in the gingivitis model. To conclude, chitosan induces moderate cytotoxic effects alone or with hemostatic gelatin at 0.19%, in dose-dependent manner, with anti-inflammatory effects on human gingival fibroblasts. The use of chitosan as a biomaterial can be an excellent choice for use in regenerative dentistry.
RESUMEN El quitosano es un biopolímero con efecto bactericida/bacteriostático, biocompatible y biodegradable. Se ha utilizado en ingeniería de tejidos con el fin de reemplazar parcial o completamente los tejidos como material bioactivo o influyendo en el crecimiento celular, comúnmente, para medicina y odontología regenerativa. Evaluar el efecto citotóxico y antiinflamatorio del quitosano solo o con gelatina hemostática (Spongostand®) en cultivos con células pulpares humanas (HPC), fibroblastos gingivales humanos (HGF) y preosteoblastos de ratón (MC3T3-E1, ATCC). HPC, HGF se aislaron de pacientes. Las células se subcultivaron en DMEM. Se inoculó quitosano a diferentes concentraciones (0-0,5%) y se colocaron gelatinas hemostáticas impregnadas con quitosano (0,19%) directamente en presencia de células y se incubaron durante 24 horas. La viabilidad celular se determinó mediante el método MTT y se calculó la concentración citotóxica media (CC50) a partir de la curva dosis-respuesta. El efecto antiinflamatorio se calculó a partir del modelo de gingivitis in vitro inducido con interleucina 1β (IL-1β) en HGF. Los datos se sometieron a las pruebas de Shapiro-Wilk, Kruskal-Wallis y Mann-Whitney. Los experimentos se realizaron por triplicado de tres ensayos independientes. La viabilidad celular de HPC, HGF y MC3T3-E1 en contacto con el quitosano disminuyó significativamente la viabilidad celular (p <0.05). Las HPC fueron las más sensibles (CC50= 0,18%) seguido de HGF (CC50= 0,18%) y MC3T3-E1 (CC50= 0,19%). Las gelatinas impregnadas con quitosano mostraron una disminución en la viabilidad celular para HGF, HPC de 11% y 5% respectivamente y se redujo significativamente el efecto pro-inflamatorio en el modelo de gingivitis humano. El quitosano induce efectos citotóxicos moderados solo o con gelatina hemostática a 0,19% de forma dosis-dependiente con efectos antiinflamatorios en fibroblastos gingivales humanos. El uso de quitosano como biomaterial puede ser una excelente opción para su uso en odontología regenerativa.
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Objective: To determine the cytotoxicity and effects of graphene oxide (GO) on cellular proliferation of gingival-fibroblasts, pulp-dental cells and human osteoblasts in culture, and to determine the physical, mechanical and biological properties of poly (methyl methacrylate) (PMMA) enriched with GO. Material and Methods: The GO was characterized with SEM. Cytotoxicity and cell proliferation were determined by the MTT bioassay. The physical mechanical tests (flexural strength and elastic modulus) were carried out with a universal testing machine. Sorption and solubility were determined by weighing before and after drying and immersion in water. Porosity was evaluated by visual inspection. Data were analyzed with Student's t-test and Tukey's posthoc ANOVA. Results: The GO has a heterogeneous morphology and a particle size of 66.67±64.76 µm. GO has a slight to no-cytotoxicity (>50-75% viability) at 1-30 days, and at 24 hours incubation of PMMA with GO significantly stimulates osteoblasts (45±8%, p<0.01). The physical and mechanical properties of PMMA with GO increase considerably without altering sorption, solubility and porosity. Conclusion: GO alone or with PMMA has an acceptable biocompatibility, could contribute to cell proliferation, cell regeneration and improve the physical mechanical properties of PMMA.
Objective: To determine the cytotoxicity and effects of graphene oxide (GO) on cellular proliferation of gingival-fibroblasts, pulpdental cells and human osteoblasts in culture, and to determine the physical, mechanical and biological properties of poly (methyl methacrylate) (PMMA) enriched with GO. Material and Methods: T he G O w as c haracterized with SEM. Cytotoxicity and cell proliferation were determined by the MTT bioassay. The physical-mechanical tests (flexural strength and elastic modulus) were carried out with a universal testing machine. Sorption and solubility were determined by weighing before and after drying and immersion in water. Porosity was evaluated by visual inspection. Data were analyzed with Student's t-test and Tukey's post-hoc ANOVA. Results: The GO has a heterogeneous morphology and a particle size of 66.67±64.76 ?m. GO has a slight to no-cytotoxicity (>50-75% viability) at 1-30 days, and at 24 hours incubation of PMMA with GO significantly stimulates osteoblasts (45±8%, p<0.01). The physical and mechanical properties of PMMA with GO increase considerably without altering sorption, solubility and porosity. Conclusion: GO alone or with PMMA has an acceptable biocompatibility, could contribute to cell proliferation, cell regeneration and improve the physical-mechanical properties of PMMA.
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Humanos , Materiais Biocompatíveis , Polimetil Metacrilato/química , Grafite/química , Osteoblastos , Óxidos , Regeneração , Bioensaio , Proliferação de Células , Resistência à FlexãoRESUMO
RESUMEN: Introducción: Uno de los procedimientos realizados con mayor frecuencia para el restablecimiento de la estética en la zona anterosuperior es el alargamiento de corona, el cual está indicado ante la presencia de una longitud insuficiente de la corona clínica de un diente, cuando existe caries o fracturas subgingivales y para la mejora de la estética en pacientes con margen gingival desigual. Objetivo: El objetivo de este artículo es presentar el alargamiento de corona estético realizado en una paciente que presentaba márgenes discrepantes y pérdida de las papilas en la zona anterosuperior, la cual fue remitida del área de prótesis para poder realizar posterior rehabilitación protésica. Resultados: Mediante el procedimiento quirúrgico se logró la corrección y restablecimiento de los márgenes gingivales. Conclusiones: La zona anterosuperior es el área estéticamente más comprometida, por lo que es indispensable la planeación interdisciplinaria del tratamiento para asegurar el éxito del mismo.
ABSTRACT: Introduction: One of the most frequently performed procedures for aesthetics restoration in the maxillary anterior area is the crown lengthening, which is indicated in the presence of an insufficient length of the clinical crown of a tooth, of cavities or subgingival fractures and for the improvement of aesthetics in patients with uneven gingival margin. Objective: The aim of this article is to present the aesthetic crown lengthening performed in a patient with discrepant margins and loss of the papillae in the maxillary anterior area, referred from the prosthesis department for subsequent prosthetic rehabilitation. Results: Through the surgical procedure, the correction and restoration of the gingival margins were achieved. Conclusions: The maxillary anterior area is the most aesthetically compromised area, which is why the interdisciplinary planning of the treatment is essential to ensure its success.
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Humanos , Feminino , Adulto , Próteses e Implantes , Prostodontia , Aumento da Coroa Clínica , Coroas , Estética DentáriaRESUMO
En la actualidad, la principal causa por la que acuden los pacientes al odontólogo es el dolor dental, endonde la mayoría presenta un padecimiento pulpar o periapical irreversibles, que pueden estar asociados a factores traumáticos e irritativos. Sin embargo, pocosde ellos son asintomáticos, como la osteítis condensante que es escasamente mencionada en elámbito de la Endodoncia; por lo tanto, el objetivo de este caso clínico es el de proporcionar información acerca de la osteítis condensante siguiendo los lineamientos internacionales de Case Report (CARE). La osteítis condensante tiene una incidencia muy baja en pacientes y se debe diagnosticar correctamente al momento de tratar este tipo de lesiones con las diferentes herramientas de diagnóstico que se conocen. En este caso, se presenta un paciente del sexo femenino de 58 años de edad con un estado prediabético, que refiere un fractura del segundo molar inferior derecho, al cual radiográficamente se le encontróuna lesión periapical radiopaca en la raíz distal. Se muestra la secuencia del tratamiento, el manejo clínico y la rehabilitación.
At present, the main reason for patients to visit adentist is dental pain, where most of them presenta pulp or periapical irreversible condition, whichmay be associated with traumatic and irritative factors. However, few of them are asymptomatic as osteitiscondensing that is barely mentioned in thefield of endodontics. The aim of this case report isto provide information about the condensing osteitisfollowing international Case Report (CARE)guidelines. Condensing osteitis has a very low incidence in patients and should be correctly diagnosed with the different available diagnostic tools. In thiscase a 58-years-old female patient, with prediabeticstate, referred of a right lower second molar fracturewhich radiographically showed a radiopaque periapicallesion in the distal root of the molar. The sequence of treatment, clinical management and rehabilitation is presented.
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Feminino , Humanos , Pessoa de Meia-Idade , Osteíte/diagnóstico , Osteíte/patologia , Osteíte/terapia , Doenças Periapicais/classificação , Tratamento do Canal Radicular/métodos , Osteosclerose/diagnóstico , Osteomielite/diagnóstico , Osteíte/diagnóstico por imagem , Coroas , Diagnóstico Diferencial , MéxicoRESUMO
The objective of this study was to determine the effects of coating nanoparticles of titanium dioxide (TiO2 NPs) and irradiation -UV on plates of titanium (Ti) for the adhesion and proliferation of human gingival fibroblasts (HGF). A total of 15 Ti plates were divided into three groups (n = 5); (i) control Ti, (ii) experimental: Ti+TiO2 NPs, (iii) experimental: Ti+TiO2 NPs+UV. The plates were analyzed with atomic force microscopy (AFM) and the roughness (Ra and Rmax) was determined. UV irradiation was performed for 20 min. HGF were subcultured in DMEM+10 % fetal bovine serum (FBS) at 37 °C with 5 % CO2. 2x106 cells/mL were inoculated on the plates and incubated for 1 h and washed with phosphate buffer saline (PBS). In the case of cell proliferation, cells were incubated for further 24 h more. Cell viability was determined with the MTT method, the formazan was dissolved with dimethylsulfoxide (DMSO) and analyzed at 540 nm. Experiments were performed of three independent experiments and data were analyzed by Kruskall-Wallis and multiple comparison of Mann-Whitney test. The surface topography of samples corresponded as follow: Ti (Ra= 0,492 µm y Rms= 0.640 µm), Ti+NPs TiO2, (Ra= 0.55 µm y Rms= 0.714 µm), respectively. The coating with TiO2 NPs significantly (p <0.05) increased the adhesion and proliferation of HGF compared with the group. The modification of Ti plates by coated with TiO2 NPs significantly increased adhesion and proliferation of HGF with the formation of a hydrophilic surface which favors the humectancy. This treatment may be reported here convenient to accelerate osseointegration of dental implants based titanium.
El objetivo fue determinar los efectos del recubrimiento con nanopartículas de dióxido de titanio (TiO2 NPs) e irradiación UV sobre placas de titanio (Ti) para la adhesión y proliferación de fibroblastos gingivales humanos (FGH). Un total de 15 placas de Ti se dividieron en tres grupos (n= 5); (i) control Ti, (ii) experimental Ti+NPs TiO2, (iii) experimental: Ti+NPs TiO2+UV. Las placas fueron analizadas en microscopía de fuerza atómica (MFA) y se determinó la rugosidad (Ra y Rmax). La irradiación con UV se realizó durante 20 min. FGH fueron subcultivados en DMEM+10 % de suero fetal bovino a 37 °C con 5 % de CO2. 2x106 células/mL fueron inoculadas sobre las placas e incubadas durante 1 h, se lavaron con solución salina de buffer fosfato. En el caso de la proliferación celular, las células se incubaron por 24 h más. La viabilidad celular se determinó con el método de MTT, el formazan fue disuelto con dimetilsulfoxido y se analizó a 540 nm. Los experimentos se realizaron a partir de tres experimentos independientes y los datos se analizaron por Kruskall-Wallis y por comparación múltiple de Mann-Whitney. La topografía de la superficie de las muestras correspondio de la siguiente manera: Ti (Ra= 0,492 µm y Rms= 0,640 µm), Ti+NPs TiO2, (Ra= 0,55 µm y Rms= 0,714 µm), respectivamente. El recubrimiento con NPs TiO2 aumentó significativamente la adhesión y proliferación de HGF en comparación con el grupo de Ti control (p <0,05). La modificación de la superficie de las placas de Ti recubiertas con NPs TiO2 aumentó significativamente la adhesión y proliferación de HGF con la formación de una superficie hidrófila que favorece la humectancia. Este tratamiento aquí informado tal vez sea un método conveniente para acelerar el proceso de la osteointegración de los implantes dentales a base de titanio.
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Humanos , Proliferação de Células/fisiologia , Fibroblastos/metabolismo , Gengiva/metabolismo , Titânio , Raios Ultravioleta , Adesão Celular , NanopartículasRESUMO
Introduction: In recent decades, dental implants have become one of the best options for comprehensive dental restoration; their placement is a multidisciplinary task that requires a solid understanding of biological, periodontal, surgical and prosthetic principles. Objective: The aim of this study was to quantify in vitro the adhesion and proliferation of human gingival fibroblasts (HGF) response on titanium (Ti) and zirconia (Zr) surfaces. Methodology: Samples of Ti and Zr were observed under atomic force microscopy (AFM). HGFs were inoculated in each sample to determine adhesion and cell proliferation. The reagent MTT was mixed with medium DMEM and inoculated in each plate; formazan was dissolved with dimethyl sulfoxide and analyzed at 540nm in a microplate spectrophotometer. The test was performed with three independent experiments. Data were analyzed with Kolmogorov-Smirnov tests (Lilliefors), Kruskal-Wallis tests and Mann-Whitney test comparisons. Results: Topography of the Zr plates showed greater roughness (Ra= 0.39 microns) than Ti (Ra= 0.049 microns). Quantification of HGF adhesion was significantly higher (p<0.05) in Ti, while proliferation showed no statistically significant differences between the groups. Conclusion: It is noteworthy that, even though Ti initially showed increased cell adhesion on the surface, after 24h Zr samples showed similar proliferation; this demonstrates that both surfaces have a comparable biological response.
Introducción: En las últimas décadas, los implantes dentales se han posicionado como una de las mejores opciones de restauración dental integral; su colocación es una tarea multidisciplinaria que requiere una sólida comprensión de los principios biológicos, periodontales, quirúrgicos y protésicos. Objetivo: El objetivo de este estudio fue cuantificar in vitro la adhesión y proliferación de la respuesta de fibroblastos gingivales humanos (HGF) en superficies de titanio (Ti) en contraste con superficies de zirconia (Zr). Metodología: Se observaron las muestras de Ti y Zr bajo microscopía de fuerza atómica (AFM). Los HGF fueron inoculados en cada muestra para determinar la adhesión y proliferación celular. El reactivo MTT se mezcló con medio DMEM y se inoculó en cada placa, el formazán se disolvió con dimetilsulfóxido y se analizó a 540nm en un espectrofotómetro de microplaca. El ensayo se realizó con tres experimentos independientes. Los datos fueron analizados con pruebas de Kolmogorov-Smirnov (Lilliefors), pruebas de Kruskal-Wallis y comparaciones de Mann-Withney. Resultados: La topografía de las placas de Zr mostraron una mayor rugosidad (Ra=0.39 micrones) en comparación con las de Ti (Ra=0.049 micrones). La cuantificación de la adhesión de HGF fue significativamente mayor (p<0.05) en el Ti, mientras que la proliferación no mostró diferencias estadísticamente significativas entre los grupos. Conclusión: Es importante mencionar que, a pesar de que el Ti mostró inicialmente una mayor adhesión celular sobre la superficie, después de 24 hrs las muestras de Zr mostraron una proliferación similar; lo que demuestra que ambas superficies poseen una respuesta biológica comparable.
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Materiais Biocompatíveis , Adesão Celular , Proliferação de Células , Implantes Dentários , Teste de Materiais , Propriedades de Superfície , Titânio/química , Zircônio/químicaRESUMO
En la actualidad existen muchos materiales dentales para la restauraciónestética, sin embargo, su tiempo de vida clínico no se conoce ampliamente. Objetivo: Identificar las publicaciones recientes de los materiales de restauración libre de metal que incluyan estudios clínicos. Metodología: La búsqueda sistemática de la literatura se realizó en bases de datos de PubMed y SciELO; se consideraron publicacionesdel 2010-2015 y que fueran investigaciones clínicas exclusivamente.Las palabras clave utilizadas fueron: Veneer crowns, dental restorationsfree metal, metal free crowns, aesthetic crowns y restauraciones libres de metal, coronas de silicato de litio, tipos de cerámicas. Resultados: Se revisaron 40 artículos y 20 cumplieron con los criterios de selección para la revisión bibliográfica. Todas las restauraciones protésicas libres de metal muestran un desempeño clínico muy similar a los tres años. Las restauraciones de zirconia y disilicato de litio por CAD mostraronel mejor éxito clínico. Conclusión: El éxito clínico de las restauracioneslibres de metal de esta revisión de la literatura muestra que van de un92.7 al 100 por ciento a tres o más años de seguimiento...
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Humanos , Cerâmica/classificação , Facetas Dentárias/estatística & dados numéricos , Estética Dentária , Restauração Dentária Permanente/estatística & dados numéricos , Zircônio/classificação , Compostos de Lítio/classificação , Desenho Assistido por Computador/métodos , Restaurações Intracoronárias , Materiais Biocompatíveis/classificação , Materiais Dentários/classificação , Óxido de Alumínio/classificação , Interpretação Estatística de Dados , Propriedades de SuperfícieRESUMO
Composite resins are the material of choice to restore minimal invasive cavities; conversely, it is important to explore the mechanical properties of commercially available dental materials. Objective: To compare the Vickers microhardness (VHN) of four available commercial composite resins using standardized samples and methods. Methodology: Composite cylinders were manufactured in a Teflon mould. We used the follow composite resins (n=4/gp): Microhybrid resins [Feeling Lux (Viarden) and AmelogenPlus (Ultradent)], Hybrid resin [Te-Econom Plus (Ivoclar)] and Nanohybridresin [Filtek Z350 (3M ESPE)]. All samples were incubated in distilled water at 37ºC for five days. The test was carried out with microhardness indenter at 10 N, and a dwelling time of 10 s for 9 indentations across the specimens resulting in a total of 36 indentations for each group. Data were subjected to Kolmogorov-Smirnov normality test and ANOVA (post-hoc) Tukey test. Results: The VHN mean values ranged from harder to softer as follows: Filtek Z350 (71.96+/-6.44) (p<0.01)> Amelogen Plus (59.90+/-4.40) (p<0.05)> Feelinglux (53.52+/-5.72)> Te-Econom Plus (53.26+/-5.19). Conclusion: According to our results, the microhardness of the evaluated conventional composite resins can withstand the masticatory forces; however nanohybrid composite resins showed better Vickers microhardness and therefore are a more clinically suitable option for minimal invasion treatments...
Las resinas son los materiales de elección para restaurar cavidades mínimamente invasivas, sin embargo, es importante conocer sus propiedades mecánicas de los materiales dentales disponibles comerciales. Objetivo: Comparar la microdureza Vickers (VHN) de cuatro resinas compuestas comerciales disponibles con muestras y métodos estandarizados. Metodología: Cilindros de resina compuesta fueron conformados en un molde de Teflón. Se utilizaron las siguientes resinas compuestas (n=4/gp): resina microhíbrida [Feeling Lux (Viarden) y Amelogen Plus (Ultradent)], resina híbrida [Te-Econom Plus (Ivoclar)] y resina nanohíbrida [Filtek Z350 (3M ESPE)]. Todas las muestras fueron incubadas en agua destilada a 37°C durante cinco días. El análisis fue realizado con un indentador de microdureza a 10 N y 10s de presión sobre la muestra, nueve indentaciones fueron realizadas a lo largo de la muestra resultando un total de 36 indentaciones por cada grupo. Los datos fueron sometidos a prueba de normalidad de Kolmogorov-Smirnov y un análisis de ANOVA (post-hoc) de Tukey. Resultados: Los valores de VHN correspondieron de la resina más dura a la más suave como se muestra a continuación: Filtek Z350 (71.96+/-6.44) (p<0.01)> Amelogen Plus (59.90+/-4.40) (p<0.05)> Feeling lux (53.52+/-5.72)> Te-Econom Plus (53.26+/-5.19). Conclusiones: De acuerdo con nuestros resultados, la dureza de las resinas compuestas convencionales evaluadas puede soportar las fuerzas masticatorias pero las resinas compuestas nanohíbridas mostraron una mejor microdureza Vickers que clínicamente puede resultar como una adecuada opción para restaurar tratamientos mínimamente invasivos...
Assuntos
Humanos , Testes de Dureza , Materiais Dentários/química , Resinas Compostas/química , Análise de Variância , Teste de Materiais , Estresse MecânicoRESUMO
The use of nanoparticles (NPs) has become a significant area of research in Dentistry. Objective The aim of this study was to investigate the physical, antibacterial activity and bond strength properties of conventional base, core build and restorative of glass ionomer cement (GIC) compared to GIC supplemented with titanium dioxide (TiO2) nanopowder at 3% and 5% (w/w). Material and Methods Vickers microhardness was estimated with diamond indenter. Compressive and flexural strengths were analyzed in a universal testing machine. Specimens were bonded to enamel and dentine, and tested for shear bond strength in a universal testing machine. Specimens were incubated with S. mutans suspension for evaluating antibacterial activity. Surface analysis of restorative conventional and modified GIC was performed with SEM and EDS. The analyses were carried out with Kolmogorov-Smirnov, ANOVA (post-hoc), Tukey test, Kruskal-Wallis, and Mann Whitney. Results Conventional GIC and GIC modified with TiO2 nanopowder for the base/liner cement and core build showed no differences for mechanical, antibacterial, and shear bond properties (p>0.05). In contrast, the supplementation of TiO2 NPs to restorative GIC significantly improved Vickers microhardness (p<0.05), flexural and compressive strength (p<0.05), and antibacterial activity (p<0.001), without interfering with adhesion to enamel and dentin. Conclusion GIC supplemented with TiO2 NPs (FX-II) is a promising material for restoration because of its potential antibacterial activity and durable restoration to withstand the mastication force. .
Assuntos
Antibacterianos/química , Colagem Dentária/métodos , Cimentos de Ionômeros de Vidro/química , Nanopartículas/química , Titânio/química , Análise de Variância , Força Compressiva , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Testes de Dureza , Teste de Materiais , Microscopia Eletrônica de Varredura , Maleabilidade , Valores de Referência , Resistência ao Cisalhamento , Estatísticas não Paramétricas , Streptococcus mutans/efeitos dos fármacos , Propriedades de SuperfícieRESUMO
Objetivo: investigar los efectos de la irradiación ultravioleta (UV) sobre placas de titanio (Ti) para la adhesión osteoblástica por medio de un método colorimétrico simple y reproducile para determinar la densidad celular. Materiales y métodos: dos diferentes tamaños (10x10x0.5 mm y 20x20x0.5 mm) de placas (n=10 c/gp) fueron obtenidas de una hoja de Ti puro y divididas en dos grupos (n=5/gp) para cada tamaño de placa. La superficie de las placas de Ti fue pulida, observada con microscopía electrónica de barrido (MEB) y estimada la rugosidad de la superficie pulida. Para el grupo experimental, las placas de Ti fueron irradiadas a una longitud de onda de 253.7 nm con luz UV durante 5, 20, 40, 60 minutos ó 4 y 6 horas. Células odontoblásticas de ratón MC3T3-E1 fueron cultivadas en medio alfa mínimo esencial (-MEM) e inoculadas sobre cada placa de Ti. El número de células adheridas y proliferadas fue determinado por medio del método MTT y el consumo de animoácidos. Resultados: 20 minutos de irradiación UV de las placas de Ti fueron suficientes para incrementar significativamente (p<0.05) la adhesión y proliferación celular, acompañado de un mayor consumo de aminoácidos. Conclusiones: la irradiación UV sobre placas de Ti incrementó significatiamente la adhesión celular por medio del método MTT, confirmando la potencialidad de la luz UV.