Assuntos
Carcinoma de Células Renais/genética , Carcinoma de Células Renais/metabolismo , Neoplasias Renais/genética , Neoplasias Renais/metabolismo , Proteínas Proto-Oncogênicas c-kit/genética , Receptor alfa de Fator de Crescimento Derivado de Plaquetas/genética , Carcinoma de Células Renais/patologia , Análise Mutacional de DNA , Humanos , Imuno-Histoquímica , Neoplasias Renais/patologia , Proteínas Proto-Oncogênicas c-kit/análise , Receptor alfa de Fator de Crescimento Derivado de Plaquetas/análiseRESUMO
BACKGROUND: Recent clinical reports suggest that intraneural needle placement may not always lead to neurologic injury. To explain the absence of neurologic complications in these reports, we studied the risk and extent of nerve injury after intentional needle-nerve placement in a cryopreserved human sciatic nerve. METHODS: The sciatic nerve was dissected from a cryopreserved cadaver through partial exposure. Needles were inserted through the nerve, using blunt-tip (30 degrees beveled) (group A) and sharp-tip (15 degrees beveled) (group D) needles. Five needle insertions were made for each needle type. Subsequently, transverse nerve sections at 10 needle trajectories were processed. Nerve samples were stained with hematoxylin-eosin, Masson trichromic, and immunohistochemical stains. In each section, the following variables were quantified: total number of fascicles and vessels in the immediate vicinity of the needle trajectories and the number of injured fascicles and vessels. RESULTS: A total of 520 fascicles were quantified, of which 134 were in contact with the needle trajectories. The numbers of fascicles and vessels per section were 65 +/- 8 and 14 +/- 7, respectively. A mean of 16 +/- 5 fascicles were found in contact with the needle trajectory (group A: 17+/- 3, group D: 15 +/- 6). Of these, 4 fascicles (3.2%) and 1 intraneural vessel were found damaged in group D. No fascicular or vascular injuries were found in group A. CONCLUSIONS: Our findings suggest that intraneural needle insertion may more commonly result in interfascicular rather than intrafascicular needle placement.
Assuntos
Ferimentos Penetrantes Produzidos por Agulha/patologia , Nervo Isquiático/lesões , Nervo Isquiático/patologia , Idoso , Cadáver , Criopreservação , Desenho de Equipamento , Humanos , Injeções/efeitos adversos , Injeções/instrumentação , AgulhasRESUMO
Benign and malignant tumors of the tongue base can be removed by various surgical approaches. A rare case of neurilemmoma of the base of the tongue removed by transoral laser microsurgery (TLM) is presented. Schwannomas in this area usually present as a slow-growing, painless mass on the tongue surface. In this case, fiberoptic examination and magnetic resonance imaging were crucial as first step studies to elucidate the biology of the lesion.