Holistic approach to waste mobil oil bioremediation: Valorizing waste through biosurfactant production for soil restoration.

The combustion of mobil oil leads to the emission of toxic compounds in the environment. In this study, the aromatic and aliphatic hydrocarbon fractions present in a waste mobil oil collected from automobile market were comprehensively identified and their toxicity was evaluated using wheat grain. Lysinibacillus sphaericus strain IITR51 isolated and characterized previously could degrade 30-80% of both aliphatic and aromatic hydrocarbons in liquid culture. Interestingly, the strain IITR51 produced 627 mg/L of rhamnolipid biosurfactant by utilizing 3% (v/v) of waste mobil oil in the presence of 1.5% glycerol as additional carbon source. In a soil microcosm study by employing strain IITR51, 50-86% of 3-6 ring aromatic hydrocarbons and 63-98% of aliphatic hydrocarbons (C8 to C22) were degraded. Addition of 60 µg/mL rhamnolipid biosurfactant enhanced the degradation of both aliphatic and aromatic hydrocarbons from 76.88% to 61.21%-94.11% and 78.27% respectively. The degradation of mobil oil components improved the soil physico-chemical properties and increased soil fertility to 64% as evident by the phytotoxicity assessments. The findings indicate that strain IITR51 with degradation capability coupled with biosurfactant production could be a candidate for restoring hydrocarbon contaminated soils.

Assessing the half-life and degradation kinetics of aliphatic and aromatic hydrocarbons by bacteria isolated from crude oil contaminated soil.

Pollution from the oil industries and refineries has worsened various environmental compartments. In this study, indigenous oil degrading bacteria were isolated from crude oil obtained from an Oil and Natural Gas Corporation (ONGC) asset in Ankleshwar, Gujarat, India. Based on 16S rRNA phylogeny, they were identified as Pseudomonas boreopolis IITR108, Microbacterium schleiferi IITR109, Pseudomonas aeruginosa IITR110, and Bacillus velezensis IITR111. The strain IITR108, IITR109, IITR110, and IITR111 showed 80-89% and 71-78% degradation of aliphatic (C8-C40) and aromatic (4-5 ring) hydrocarbons respectively in 45 d when supplemented with 3% (v/v) waste crude oil. When compared to individual bacteria, the consortium degrades 93.2% of aliphatic hydrocarbons and 85.5% of polyaromatic hydrocarbons. It was observed that the total aliphatic and aromatic content of crude oil 394,470 µg/mL and 47,050 µg/mL was reduced up to 9617.75 µg/mL and 4586 µg/mL respectively in 45 d when consortium was employed. The rate kinetics analysis revealed that the biodegradation isotherm followed first order kinetics, with a linear correlation between concentration (hydrocarbons) and time intervals. The half-life of aliphatic (C8-C40) and aromatic hydrocarbons ranged from 200 to 453 h and 459-714 h respectively. All the bacteria efficiently produced catabolic enzymes such as alkane monooxygenase, alcohol dehydrogenase, and lipase during the degradation of crude oil. These findings indicated that the bacterial consortium can be a better candidate for bioremediation and reclamation of aliphatic and aromatics hydrocarbon contaminated sites.

Multi-omics approaches for remediation of bisphenol A: Toxicity, risk analysis, road blocks and research perspectives.

In this "plastic era" with the increased use of plastic in day today's life the accumulation of its degraded products like microplastics or plastic additives such as Bisphenol A(BPA) is also increasing. BPA is an endocrine-disrupting chemical used as a plasticizing agent in clear plastic, building materials, coatings, and epoxy resin. Several enzymes including laccases and lipases have been studied for the reduction of BPA toxicity. Over the decades of encountering these toxicants, microorganisms have evolved to degrade different classes of plastic additives. Since the degradation of BPA is a long process thus meta-omics approaches have been employed to identify the active microbiota and microbial dynamics involved in the mitigation of BPA. It is also necessary to investigate the impact of processing activities on transit of BPA in food items and to limit its entrance in food world. This review summarizes a comprehensive overview on BPA sources, toxicity, bio-based mitigation approaches along with a deeper understanding of multi-omics approaches for its reduction and risk analysis. Knowledge gaps and opportunities have been comprehensively compiled that would aid the state-of-the-art information in the available literature for the researchers to further address this issue.

Production of biosurfactants from agro-industrial waste and waste cooking oil in a circular bioeconomy: An overview.

Waste generation is becoming a global concern owing to its adverse effects on environment and human health. The utilization of waste as a feedstock for production of value-added products has opened new avenues contributing to environmental sustainability. Microorganisms have been employed for production of biosurfactants as secondary metabolites by utilizing waste streams. Utilization of waste as a substrate significantly reduces the cost of overall process. Biosurfactant(s) derived from these processes can be utilized in environmental and different industrial sectors. This review focuses on global market of biosurfactants followed by discussion on production of biosurfactants from waste streams such as agro-industrial waste and waste cooking oil. The need for waste stream derived circular bioeconomy and scale up of biosurfactant production have been narrated with applications of biosurfactants in environment and industrial sectors. Road blocks and future directions for research have also been discussed.

Sustainable processing of food waste for production of bio-based products for circular bioeconomy.

Sustainable development of circular bioeconomy concept is only possible upon adopting potential advanced technologies for food waste valorization. This approach can simultaneously answer resources and environmental challenges incurred due to capital loss and greenhouse gases accumulation. Food waste valorization opens new horizons of economical growth, bringing waste as an opportunity feedstock for bio processes to synthesize biobased products from biological source in a circular loop. Advanced technologies like Ultrasound assisted extraction, Microwave assisted extraction, bioreactors, enzyme immobilization assisted extraction and their combination mitigates the global concern caused due to mismanagement of food waste. Food waste decomposition to sub-zero level using advanced techniques fabricates food waste into bio-based products like bioactive compounds (antioxidants, pigments, polysaccharides, polyphenols, etc.); biofuels (biodiesel, biomethane, biohydrogen); and bioplastics. This review abridges merits and demerits of various advanced techniques extended for food waste valorization and contribution of food waste in revenue generation as value added products.

Biocatalytic remediation of industrial pollutants for environmental sustainability: Research needs and opportunities.

An increasing quantum of pollutants from various industrial sector activities represents a severe menace to environmental & ecological balance. Bioremediation is gaining flow globally due to its cost-effective and environment-friendly nature. Understanding biodegradation mechanisms is of high ecological significance. Application of microbial enzymes has been reported as sustainable approach to mitigate the pollution. Immobilized enzyme catalyzed transformations are getting accelerated attention as potential alternatives to physical and chemical methods. The attention is now also focused on developing novel protein engineering strategies and bioreactor design systems to ameliorate overall biocatalysis and waste treatment further. This paper presents and discusses the most advanced and state of the art scientific & technical developments about biocatalytic remediation of industrial pollutants. It also covers various biocatalysts and the associated sustainable technologies to remediate various pollutants from waste streams. Enzyme production and immobilization in bioreactors have also been discussed. This paper also covers challenges and future research directions in this field.

Distribution of heavy metals in sediment and water of river Gomti.

Gomti river receives industrial as well as domestic wastes from various drains of Lucknow city. In the process the water and sediment of the river Gomti get contaminated with heavy metals and other pollutants. In the present study, impacts of domestic/industrial wastes on the water and sediment chemistry of river Gomti with special reference to heavy metals have been investigated in different seasons (summer, winter and rainy). For this, seven sampling sites: Gaughat, Mohan Meakin, Martyr's Memorial, Hanuman Setu, Nishatganj bridge, Pipraghat and Malhaur, in the river Gomti in Lucknow region were identified and samples of water and sediments were collected in all the three seasons. In the collected water and sediment samples, six metals (Cd, Cr, Cu, Ni, Pb, and Zn) were analyzed on ICP-AES (Inductively coupled plasma emission spectroscopy) Labtam Plasmalab 8440. High concentrations of all the metals were noticed in water and sediment in rainy season compared to summer and winter. Because in rainy season runoff from open contaminated sites, agricultural field and industries, directly comes into the river without any treatment. In both the cases, the concentration of zinc was maximum (0.091 microg/ml in water and 182.13 microg/g in sediment) and the concentration of cadmium (0.001 microg/ml in water and 17.26 microg/g in sediment) was minimum. Higher concentration of metal in water and sediment during rainy season could be due to the industrial/agricultural/domestic runoff coming into the river.