Effects of dietary curcumin nano-micelles on performance, biological responses, and thermal stress resilience in heat-stressed fattening lambs across varying temperature-humidity index conditions: Implications for climate change.

Heat stress poses a significant challenge to sheep farming in arid and semi-arid regions, impacting growth performance, health, and physiological responses. While sheep have innate mechanisms to manage heat stress, prolonged exposure impairs their performance and health. This study evaluated the influence of varying doses of Curcumin Nano-Micelle (CNM) on heat-stressed fattening lambs in northeastern Iran over three months, examining the relationship between CNM doses and growth performance, feeding behavior, physiological responses, immune function, and antioxidant status. Thirty-two crossbred male lambs were included in a completely randomized design with four treatments and eight replications. The experimental treatments were as follows: 1) CTRL: No dietary inclusion of CNM, (control group); 2) T20: Dietary inclusion of 20 mg of CNM per head per day; 3) T40: Dietary inclusion of 40 mg of CNM per head per day; and 4) T80: Dietary inclusion of 80 mg of CNM per head per day. The results revealed that dietary supplementation with 20 and 40 mg of CNM significantly improved live body weight, weight gain, average daily gain (ADG), and feed conversion ratio (FCR) compared to the control treatment. Regression analysis demonstrated quadratic models between growth performance parameters and the Temperature-Humidity Index (THI), indicating a correlation between CNM doses and the animals' responses to heat stress. Regarding eating behavior, CNM doses of 40 and 80 mg/day significantly reduced eating time while increasing ruminating time. Blood analysis indicated significant reductions in glucose levels across all treatments, with T40 significantly reducing both cholesterol and triglyceride (TG) levels. Additionally, CNM supplementation decreased serum malondialdehyde (MDA) levels and increased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, indicating enhanced antioxidant status. Physiological responses were influenced by CNM, notably reducing rectal temperature (RT), skin temperature (ST), respiration rate (RR), while pulse rate (PR) increased across various time intervals, particularly in the T80 group. This study demonstrates that CNM supplementation can enhance performance, physiological responses, and antioxidant status in heat-stressed fattening lambs, highlighting its potential to mitigate heat stress effects in sheep farming.

Evaluating the dose-dependent effects of curcumin nano-micelles on rumen fermentation, nitrogen metabolism, and nutrient digestibility in heat-stressed fattening lambs: Implications for climate change and sustainable animal production.

Global warming threatens livestock production, especially in hot climates. This study evaluated the dose-dependent impacts of dietary curcumin nano-micelles (CNM) on rumen fermentation, nitrogen metabolism, and nutrient digestibility in heat-stressed fattening lambs. Thirty-two crossbred male lambs [ˆIle-de-France × (Dalagh × Romanov)] were utilized within the current study. The initial weight of lambs was documented as averaged by 31.2 ± 1.55 kg, while they were in their 4th to 5th months of age. Animals were fed increasing doses of dietary CNM (0, 20, 40, and 80 mg/day) over 97 days, under severe heat stress conditions with average temperature-humidity index (THI) of 24.5. Quadratic improvements (p < 0.01) occurred in weight gain, average daily gain (ADG), and feed conversion ratio (FCR) by 28.7%, 27.4%, and 23.9%, respectively, in the T40 group compared to the control. Additionally, T40 increased fiber digestion by 2.8% (p < 0.05). Furthermore, T40 quadratically improved parameters of rumen fermentation, including concentrations of NH3-N (p < 0.05), total volatile fatty acids (TVFA; p < 0.01), acetate (p < 0.05), and iso-valerate (p < 0.05), by 13.9%, 12.5%, 15.0%, and 43.5%, respectively, compared to the control. Quadratic increases were also observed in nitrogen balance (p < 0.05) and microbial protein synthesis (p < 0.01) by 19.8% and 37.6%, respectively, in the T40 group. Quadratic models estimated optimal CNM levels between 41.5 and 48.6 mg/day for multiple parameters. These findings indicate CNM at dose level of 40 mg/day could benefit heat-stressed lambs through enhanced rumen function and microbiota. Further research should refine ideal dosing for various species and production phases as higher levels adversely impacted fiber digestibility. Overall, CNM shows promise as a sustainable nutritional intervention for livestock production facing global warming.

The effect of maternal organic manganese supplementation on performance, immunological status, blood biochemical and antioxidant status of Afshari ewes and their newborn lambs in transition period.

It has been documented that adequate maternal manganese (Mn) status is vital for performance and health of ewes and their newborn lambs. However, required level and form of dietary Mn in ruminants are not well defined. The current study was conducted to evaluate the effect of maternal organic Mn supplementation on performance, immunological status, blood biochemical and antioxidant status of Afshari ewes and their newborn lambs in transition period. For this purpose, various organic Mn concentrations were utilized as a supplementary ingredient in formulating the diets of ewes. The ewes were randomly allocated into three groups, fed with 0, and 80 mg/kg organic Mn supplemented diet. At the end of the experiment, the parameters including the performance of newborn lambs, as well as biochemical factors, immune status and antioxidant status in ewes and their newborn lambs were evaluated. The results showed a significant increase in the plasma concentrations of Mn, glucose, insulin, thyroid hormones (T3 , T4 ) and enzymatic antioxidants (SOD, GPX , CAT) in ewes and their newborn lambs that were treated with maternal organic Mn. Moreover, inorganic Mn treatments, the concentration of IgG in newborn lamb's plasma, and colostrum of ewes increased. According to this research, organic Mn acts as a valuable and safe supplementary material that could be exploited for enhancing health of ewes and their newborn lambs.

Quantitative Associations between Season, Month, and Temperature-Humidity Index with Milk Yield, Composition, Somatic Cell Counts, and Microbial Load: A Comprehensive Study across Ten Dairy Farms over an Annual Cycle.

This current study addresses the knowledge gap regarding the influence of seasons, months, and THI on milk yield, composition, somatic cell counts (SCC), and total bacterial counts (TBC) of dairy farms in northeastern regions of Iran. For this purpose, ten dairy herds were randomly chosen, and daily milk production records were obtained. Milk samples were systematically collected from individual herds upon delivery to the dairy processing facility for subsequent analysis, including fat, protein, solids-not-fat (SNF), pH, SCC, and TBC. The effects of seasons, months, and THI on milk yield, composition, SCC, and TBC were assessed using an analysis of variance. To account for these effects, a mixed-effects model was utilized with a restricted maximum likelihood approach, treating month and THI as fixed factors. Our investigation revealed noteworthy correlations between key milk parameters and seasonal, monthly, and THI variations. Winter showed the highest milk yield, fat, protein, SNF, and pH (p < 0.01), whereas both SCC and TBC reached their lowest values in winter (p < 0.01). The highest values for milk yield, fat, and pH were recorded in January (p < 0.01), while the highest protein and SNF levels were observed in March (p < 0.01). December marked the lowest SCC and TBC values (p < 0.01). Across the THI spectrum, spanning from -3.6 to 37.7, distinct trends were evident. Quadratic regression models accounted for 34.59%, 21.33%, 4.78%, 20.22%, 1.34%, 15.42%, and 13.16% of the variance in milk yield, fat, protein, SNF, pH, SCC, and TBC, respectively. In conclusion, our findings underscore the significant impact of THI on milk production, composition, SCC, and TBC, offering valuable insights for dairy management strategies. In the face of persistent challenges posed by climate change, these results provide crucial guidance for enhancing production efficiency and upholding milk quality standards.

Effects of dietary curcumin nano-micelles on growth performance, blood metabolites, antioxidant status, immune and physiological responses of fattening lambs under heat-stress conditions.

The aim of the current study was to investigate the effects of dietary curcumin nano-micelles (C-NM) on the growth performance, blood metabolites, antioxidant status, and immune and physiological responses of fattening lambs under heat stress conditions. Thirty-two crossbred male lambs [Île-de-France × (Dalagh × Romanov)] with an average weight of 31.2 ± 1.55 kg and age of 4-5 months were assigned to experimental treatments in a completely randomized design including four treatments and eight replications. The treatments were T0, T20, T40, and T80, representing dietary supplementation of C-NM at 0, 20, 40, and 80 mg per head per day, respectively. The study lasted for 37 days including 7 days of adaptation period. Lambs were weighed on days 0 and 30 of the experiment before morning feeding to determine the average daily gain (ADG) and feed conversion ratio (FCR). Blood samples were collected from the jugular vein on day 30 of the experiment and physiological parameters, including rectal temperature (RT), skin temperature (ST), respiration rate (RR), and pulse rate (PR), were measured once a week on a certain day during the study. Lambs in the T40 group showed higher final live weight (FLW) and ADG, while exhibiting lower FCR (P < 0.01). Regression analysis predicted the optimum levels of dietary inclusion of C-NM to be 44.7, 38.3, and 42.0 mg/day for FLW, ADG, and FCR, respectively. Dietary supplementation of C-NM decreased RT, ST, and RR, while increasing PR (P < 0.01). The levels of immunoglobulins G (IgG) and A (IgA) increased linearly with the dietary inclusion of C-NM (P < 0.01), while levels of immunoglobulin M (IgM) remained unaffected (P > 0.05). Dietary inclusion of C-NM had a quadratic reducing effect on the serum concentration of malondialdehyde (MDA) and a cubic increasing effect on the serum activities of glutathione peroxidase (GPx) (P < 0.05). Superoxide dismutase (SOD) was linearly increased in T80, while total antioxidant capacity (TAC) showed a linear increase in T40 and T80 groups (P < 0.01). Based on the results of this study, we recommend the administration of C-NM in the diet of fattening lambs during hot months in tropical and subtropical areas. However, further studies are needed to assess the long-term effects of C-NM during various physiological and production statuses.

The effect of different processing methods of linseed on growth performance, nutrient digestibility, blood parameters and ruminate behaviour of lambs.

BACKGROUND: Oilseeds such as linseed, canola and sunflower contain unsaturated fatty acids that play important functions in the body. The aim of this study was to assess the effects of different levels of processing linseed on growth performance, nutrient digestibility, blood parameters and ruminate behaviour of lambs. METHODS: Fifty-six Moghani male lambs (3 months of age, initial average body weight = 28 ± 1.2 kg) were allocated to seven experimental diets in randomized design (eight lambs per each treatment). The experimental diets were as follows: (1) control diet (without linseed), (2) 5% raw linseed, (3) 10% raw linseed, (4) 5% micronized linseed, (5) 10% micronized linseed, (6) 5% extruded linseed and (7) 10% extruded linseed. Lambs were fed ad libitum a basal diet as total mixed ration consisting of 25% concentrate and 75% hay. RESULTS: The results showed that linseed level and processing method had no significant effect on dry matter intake. Average daily gain, final body weight and feed conversion ratio (FCR) in lambs were affected by experimental diets. The use of 10% micronized linseed and 10% of extruded linseed in the lambs' diet improved dry matter and crude protein digestibility significantly (p < 0.001). Blood glucose concentration observed for lambs fed 10% of micronized or extruded linseed (LS) was not different from that observed in other groups, only from the values shown by lambs fed diets 1 (control) and 2 (5% raw LS). The lowest cholesterol and the highest blood urea nitrogen concentrations were related to lambs fed the control diet (p < 0.001). Feeding processed linseed relative to control diet had no effect on feeding behaviour in lambs. CONCLUSION: Results of this research showed that the use of extruded and micronized linseed at the level of 10% can improve FCR, nutrient digestibility, and blood parameters.

Effects of Environmental Temperature and Humidity on Milk Composition, Microbial Load, and Somatic Cells in Milk of Holstein Dairy Cows in the Northeast Regions of Iran.

The present study aims to examine the relationships between temperature and humidity and milk composition, microbial load, and somatic cells in the milk of Holstein dairy cows. For this purpose, the temperature−humidity index, ambient temperature, and relative humidity data were obtained from the nearest weather stations. Production data were obtained from four dairy farms in Golestan province, Iran, collected from 2016 to 2021. The traits investigated were protein, fat, solids-not-fat (SNF), microbial load, and somatic cell count (SCC) in milk. The effects of the environmental temperature, humidity, month, and season on the milk composition, microbial load, and somatic cells were analyzed through analysis of variance. The effects of environmental temperature, humidity, month, and season on the milk composition, microbial load, and somatic cell composition were analyzed using a mixed procedure with a restricted maximum likelihood model. Although our findings revealed that there were significant differences in fat, protein, SNF, and SCC among the different months of the year (p < 0.01), no significant difference was observed in the total microbial count in milk. Environmental temperature presented significant impacts on fat, protein, SNF, SCC, and total microbial count within various temperature ranges (p < 0.01). When the temperature increased from 6.2 °C to 31.3 °C, the milk protein, fat, SNF, and somatic cell count significantly decreased, by approximately 4.09%, 5.75%, 1.31%, and 16.8%, respectively; meanwhile, the microbial count in milk significantly increased, by approximately 13.7%. Humidity showed an influence on fat, protein, non-fat solids, somatic cells, and total microbial count within different temperature ranges (p < 0.01). When the humidity increased from 54% to 82%, the milk protein, fat, SNF, and SCC significantly increased, by approximately 3.61%, 4.84%, 1.06%, and 10.2%, respectively; meanwhile, the microbial count in milk significantly decreased, by approximately 16.3%. The results demonstrate that there is a negative correlation between different months of the year, temperature, and the humidity of the environment, in terms of milk components and SCC. Our findings demonstrate that the optimum performance, in terms of milk composition, occurred in the first quarter of the year. As temperature increases and humidity decreases, milk quality decreases. Therefore, the adverse effects of environmental conditions on agricultural profits are not negligible, and strategies to better deal with the negative environmental effects are needed in order to improve milk quality in dairy cows.

Research Note: Effect of corn silage and alfalfa meal as alternative induced molt methods to improving Salmonella Enteritidis resistance in laying hens.

This experiment was conducted to evaluate diets containing a high level of corn silage and alfalfa meal in inducing molt and reducing susceptibility to Salmonella Enteritidis (SE) colonization in laying hens. Thirty-two healthy hens were examined by cloacal swab samples to be free of Salmonella. Then they were weighed individually and distributed to 4 experimental groups containing 8 hens each, including Full-fed (control, FF); total feed withdrawal (positive control for molt induction, FW); 80% corn silage (CS) + 20% layer diet (CS80), and 80% alfalfa meal (AM) + 20% layer diet (AM80). The molting program was initiated at 71 wk of age. On d 4 of the experiment, all hens were inoculated with SE by oral gavage. All hens were first weighed at the ending molting period on d 10 and then euthanized by CO2 gas. The internal organs including the ovary, oviduct, liver, and spleen, were excised aseptically and weighed. Cloacal swab and feed samples at the beginning and organ samples (liver, ovary, spleen, and cecum) were collected from each hen at the end of the experiment and examined for SE colonies. Molted birds lost roughly 14 to 27%t of their body weight and had significantly lower organ weight and egg production compared to FF group (P < 0.05). No significant difference was observed in the number of days to zero egg production between molted treatments. The SE positive organs did not significantly differ between CS80 and AM80 with FF treatment. Treatment CS80 had the lowest crop pH and differed substantially from treatment FW. In conclusion, results indicate that using corn silage and alfalfa meal, can improve resistance to salmonella Enteritidis during molt inducing compared to traditional feed withdrawal.

Preparation of fish waste silage and its effect on the growth performance and meat quality of broiler chickens.

BACKGROUND: This experiment was conducted to evaluate the effects of replacing soybean meal with fish-waste silage (FWS) in diets on growth performance and meat quality in broiler chickens. Fish waste (FW) was fermented with molasses and Lactobacillus plantarum and Aspergillus oryzae for 15 days. A total of 240 day-old male Cobb 500 broiler chickens were used in a 42-day experiment in which the birds were randomly allotted to one of five dietary treatments (containing 0, 30, 60, 90, and 120 g kg-1 FWS) in a completely randomized design. RESULTS: Results indicated that the fermentation process significantly reduced pH and ether extract, but it increased crude protein content and lactic acid bacteria count in FW. The use of FWS significantly improved daily weight gain and feed conversion ratio of broilers. The amount of thiobarbiturate-reducing substances and lipid content in chicken's meat fed diet containing FWS was significantly lower than in the group fed a control diet. CONCLUSION: In conclusion, the microbial fermentation process is effective in improving nutritive values of FW. Feeding up to 120 g kg-1 FWS also improves the performance and meat quality of birds and can be used as a suitable protein source in broiler chickens' diet. © 2018 Society of Chemical Industry.

Effects of monensin and increasing crude protein in early lactation on performance of dairy cows.

Twenty-four Holstein dairy cows were used to evaluate the singular and combined effects of different level of crude protein and monensin treatments during the early lactation on digestion and milk yield of dairy cows. The experiment was designed as completely randomized with a 3x2 factorial arrangement of treatments. The factors were three Concentrations of CP supplement (19.5, 21.4 and 23.4% of dry matter) and two levels of monensin (0 and 350 mg per cow per day). This experiment consist of three periods and each period was 3 week in length. Monensin did not affect DMI, milk yield, lactose and SNF but it reduced milk fat and protein percentage. Monensin premix significantly decreased rumen ammonia but rumen pH and microbial protein synthesis was not affected by monensin treatment. Although, Monensin treatment increased apparent digestibility of DM, NDF, ADF, CP, but they were not significantly. Increasing dietary CP, improved milk and protein production, but did not alter the other components of milk. Digestibility of NDF, ADF, CP were improved by increasing dietary CP. Increasing diet CP from 19.5 to 21.4% did not significantly increase ruminal ammonia, but increasing to 23.4% have significant effect on it. There was a linear relationship between level of crud protein in the diet and urine volume excretion. Microbial protein synthesis was affected by increasing CP level; on this way maximum protein synthesis was achieved in 21.4% CP.