[The biorhythmology of primary and superinvasive opisthorchiasis. The seasonal variability in the relative mass of the parenchymatous and lymphoid organs in golden hamsters]. / Bioritmologiia pervichnogo i superinvazionnogo opistorkhoza. Sezonnaia izmenchivost' otnositel'noi massy parenkhimatoznykh i limfoidnykh organov zolotistykh khomiakov.

600 Golden Hamsters were divided into 3 groups: 1--free from opisthorchis invasion, 2--once infected, 3--repeatedly infected. Seasonal variability of liver, portal and mesentery lymph node and adrenal gland relative mass was investigated in winter, spring and autumn. Diurnal stereotype variability of parenchymatous and lymphoid organ relative mass is associated with a season and invasion number, and in a considerable degree is reflected in the meaning of average diurnal index.

[The biorhythmology of primary and superinvasive opisthorchiasis. Seasonal changes in the circadian activity of the cyclic nucleotides and of the key enzyme of the pentosephosphate cycle (G-6-PD) in the peripheral blood of golden hamsters]. / Bioritmologiia pervichnogo i superinvazionnogo opistorkhoza. Sezonnye izmeneniia sutochnoi aktivnosti tsiklicheskikh nukleotidov i kliuchevogo fermenta pentozo-fosfatnogo tsikla (G-6-FDG) perifericheskoi krovi zolotistykh khomiakov.

570 males of golden hamsters were divided into 3 groups: I--free from invasion, II--once infected, III--repeatedly infected. Diurnal activity of cyclic nucleotides and enzyme of pentose-phosphate cycle (G-6-PhDG) was investigated in spring, autumn, and winter at 3, 7, 11 a.m. and 3, 7, 11 p.m. The golden hamsters once infected with opisthorchiasis do not show any seasonal changes in G-6-PhDG activity, but this invasion influences the total content of protein, diurnal oscillation of which depends upon seasons. In spring time the repeated invasion induces the diurnal rhythms of G-6-PhDG and of total protein content. Diurnal stereotype of cyclic nucleotides (cGMP, cAMP) demonstrates the dependence upon seasons and invasion frequency without any changes of the average diurnal level.

[The biorhythmology of primary and superinvasive opisthorchiasis. The seasonal changes in the circadian activity of the portal (regional) lymph node in golden hamsters]. / Bioritmologiia pervichnogo i superinvazionnogo opistorkhoza. Seszonnye izmeneniia sutochnoi aktivnosti portal'nog (regional'nogo) limfaticheskogo uzla zolotistykh khomiakov.

540 male golden hamsters were divided into 3 groups: I--free from invasion, II--once infected, III--repeatedly infected. Diurnal activity of portal lymph node immunocompetent cells was investigated in spring, autumn and winter at 3, 7, 11 a. m. and 3, 7, 11 p. m Peculiarities of diurnal immunocompetent cell activity at on acute opisthorchiasis stage, depending upon a season invasion frequency is determined by immunoreactivity level. Eosinophilic and lymphocytic reactions and the ratio of mitotic activity to degenerative elements are the lymph-node immunocompetence indices.

[The biorhythmology of primary and superinvasive opisthorchiasis. Seasonal changes in the circadian dynamics of the peripheral blood composition in golden hamsters]. / Bioritmologiia pervichnogo i superinvazionnogo opistorkhoza. Sezonnye izmeneniia sutochnoi dinamiki sostava perifericheskoi krovi zolotistykh khomiakov.

540 golden hamsters were selected into 3 groups: 1--free from invasion, 2--once infected, 3--repeatedly infected with Opisthorchis felineus. Diurnal activity of peripheral blood composition was investigated in spring, autumn and winter at 3, 7, 11 a.m. and 3, 7, 11 p.m. The dependence of biorhythmical organisation of peripheral blood cell composition upon seasons and the opisthorchis invasion frequency was recovered. The eosinophilia is the marker reaction of opisthorchiasis acute phase and it depend upon the invasion frequency. Seasons and invasion frequency affect on all diurnal rhythm components (mezor, acrophase, amplitude) with most largest drift in spring.

[The comparative characteristics of the clinico-immunological indices in different contingents of opisthorchiasis patients in a hyperendemic focus. 4. The transplacental transmission of Opisthorchis felineus antigens]. / Sravnitel'naia kharakteristika kliniko-immunologicheskikh pokazatelei raznykh kontingentov bol'nykh opistorkhozom v giperéndemichnom ochage. Soobshchenie 4. Transplatsentarnaia peredacha antigenov Opisthorchis felineus.

25 women in labour with chronic opisthorchiasis have been examined. They were representatives of native (7), local (8) and migrated population of the focus. Control group comprised 10 women from the local population free of invasion. Their immune status was assessed by the level of IgA, IgG, IgM, circulating immune complexes (CIC) and specific antibodies in the serum obtained from peripheral and funic veins, as determined by enzyme immunoassay (ELISA). To detect opisthorchis antigens in the serum modified radial immunodiffusion test was used. Antibody titres in ELISA, IgG and CIC levels were the highest in migrated women. Antibody titres in ELISA and IgG level in the newborn of migrated women were also considerably higher than in other newborn. Serum IgM level in all groups of newborn was considerably lower than in their mothers and IgA was altogether undetectable. In 3 native and 2 local women the results of immunodiffusion test were positive. It is suggested that transplacental migration of opisthorchis throughout the neonatal period may lead to tolerance formation.

[The dynamics of the liver calcium-binding protein in golden hamsters with opisthorchiasis]. / Dinamika kal'tsiisviazyvaiushchego belka pecheni zolotistykh khomiakov pri opistorkhoze.

By means of immunochemical methods a considerable increase in Ca(2+)-transporting glycoprotein (GP) in liver tissues of golden hamsters at early stages of opisthorchiasis has been established. On the 3rd day after the infection GP amount increases 6 fold, on the 7th day--15 fold. On the 14th day GP amount decreases but does not reach the control level. The control level is reached only on the 22nd day after the infection of animals. Ca(2+)-transporting GP belongs to the system of electrogenic transport of Ca2+ in mitochondria (MCh) and its increase in MCh of the liver is, apparently, the basis of destructive changes of these organelles.

[The decreased susceptibility of Syrian hamsters to Opisthorchis felineus invasion after its repeated administration]. / Snizhenie vospriimchivosti siriiskikh khomiakov k invazii Opisthorchis felineus posle povtornogo vvedeniia.

The investigations conducted on Syrian hamsters have revealed that multiple enteral infusions of cold inactivated (t = -10 degrees C) O. felineus metacercariae resulted in the formation of immunocompetent cell and fibroblast infiltrations around the branches of the portal vein. The infestation of animals by viable pathogens led to reduced accommodation of helminths and suppressed cell infiltration in the liver. It is assumed that the native population of the foci become tolerant to the disease with the penetration of noninvasive Opisthorchis larvae into their body and the development of latent forms of helminthiasis.

[Effect of an Opisthorchis infestation on implantation growth of the liver epithelium]. / Vliianie opistorkhoznoi invazii na implantatsionnyi rost épiteliia pecheni.

The features of proliferation and differentiation of the liver ductular epithelium from animals infected with opisthorchis were specified in experimental implantation growth. They manifested themselves in disorders of the mitotic regimen and intercellular interactions, in atypical proliferations and primary glandular metamorphism of regenerating cells.

[Circadian rhythm of liver phospholipids in the normal golden hamster and in opisthorchiasis]. / Sutochnyi ritm fosfolipidov pecheni zolotistogo khomiaka v norme i pri opistorkhoze.

Circadian rhythms of total phospholipids, individual fractions of phosphatide acid, cardiolipin, phosphatidyl ethanolamine, phosphatidylcholine, sphingomyelin, and lysolecithins in the liver of golden hamsters infected with opisthorchiasis (days 30 and 150 of invasion) and freed from helminths (day 92 after dehelminthization) are desynchronized in phase, amplitude and level.