Oxidative stress and antioxidant defence responses in two marine copepods in a high CO2 experiment.

We collected samples for oxidative stress and antioxidants in a high CO2 mesocosm experiment for two weeks, focussing on two common crustacean copepods Calanus finmarchicus and Temora longicornis. The samples were collected during a field experiment campaign studying responses of plankton communities to future ocean acidification (OA), off the Norwegian coast south of Bergen. The main results showed that there were species-specific differences between Temora and Calanus, especially in antioxidant defences (glutathione system) and oxidative stress (lipid peroxidation and reduced:oxidised glutathione ratio). Regular monitoring of chlorophyll a and jellyfish abundances taking place during the field campaign revealed that both chl a and predators may have affected the eco-physiological response. Antioxidant and oxidative stress levels are known to respond sensitively to both the food quality and quantity and the predator pressure, apart from environmental (i.e., abiotic) changes. Calanus was more robust towards OA, perhaps due to its high tolerance to a wide range of vertical physical-chemical conditions. Both top-down and bottom-up factors seem to play a role for the outcome of copepod responses to future ocean acidification.

Oxidative stress and antioxidant defense responses in Acartia copepods in relation to environmental factors.

On a daily basis, planktonic organisms migrate vertically and thus experience widely varying conditions in their physico-chemical environment. In the Gulf of Finland, these changes are larger than values predicted by climate change scenarios predicted for the next century (up to 0.5 units in pH and 5°C in temperature). In this work, we are interested in how temporal variations in physico-chemical characteristics of the water column on a daily and weekly scale influence oxidative stress level and antioxidant responses in the planktonic copepod of the genus Acartia. Responses were determined from samples collected during a two-week field survey in the western Gulf of Finland, Baltic Sea. Our results showed that GST (Glutathione-S-transferase) enzyme activity increased in the surface waters between Weeks I and II, indicating antioxidant defense mechanism activation. This is most likely due to elevating temperature, pH, and dissolved oxygen observed between these two weeks. During Week II also GSSG (oxidized glutathione) was detected, indicating that copepods responded to stressor(s) in the environment. Our results suggest that Acartia copepods seem fairly tolerant to weekly fluctuations in environmental conditions in coastal and estuarine areas, in terms of antioxidant defense and oxidative stress. This could be directly connected to a very efficient glutathione cycling system acting as antioxidant defense system for neutralizing ROS and avoiding elevated levels of LPX.

Changes in wintertime pH and hydrography of the Gulf of Finland (Baltic Sea) with focus on depth layers.

We studied changes in sea water pH, temperature and salinity with focus on two depth layers, along the Gulf of Finland (the Baltic Sea) using long-term monitoring data from 1979 to 2015. Data from the most frequently sampled monitoring stations between western and eastern Gulf of Finland were used. The main result of the study reveals that pH has decreased both in surface and deep-water in the western Gulf of Finland with values ranging between -0.005 and -0.008 units year-1. We also demonstrate a rise in temperature (~2 °C) and decrease in salinity (~-0.7 g kg-1) at several stations over the last 36 years. In general, the changes are shown to be more pronounced in the western part of the gulf. This paper also stresses the importance of improving the sampling frequency and quality of monitoring measurements.

Removal and Biodegradation of Phenanthrene, Fluoranthene and Pyrene by the Marine Algae Rhodomonas baltica Enriched from North Atlantic Coasts.

This study is focused on the removal, accumulation and degradation of three environmental ubiquitous polycyclic aromatic hydrocarbons (PAHs), phenanthrene (PHE), fluoranthene (FLA) and pyrene (PYR), by the marine alga Rhodomonas baltica enriched from the English Channel. After separation, purification and culture in several phases, R. baltica was exposed to PAH concentrations that are frequently encountered in the field in several anthropized environments. The results showed that R. baltica can grow under PAH stress, efficiently remove up to 70% of these compounds from the medium by 216 h of culture and selectively bioaccumulate PAHs by their hydrophobicity. Between PHE, FLA and PYR, phenanthrene was the compound with higher degradation rates throughout incubation. The equilibrium partitioning theoretical approach showed that physico-chemical partitioning, rather than active bioconcentration, was the major factor governing the bioaccumulation, outlying a potential application in decontamination processes for this species.

Molecular characterization and mRNA expression of grp78 and hsp90A in the estuarine copepod Eurytemora affinis.

The present study aimed to develop a method of quantification of heat shock protein transcript levels in the estuarine copepod Eurytemora affinis. For that, the full-length cDNA of the 78-kDa glucose-regulated protein (Ea-grp78) and the cytosolic 90-kDa heat shock protein (Ea-hsp90A) from this species have been cloned. These cDNA revealed, respectively, 2,370 and 2,299 bp with 1,971 and 2,124 bp open reading frames encoding 656 and 707 amino acids. Main features, sequence identities and phylogenetic analysis with other species were described. Then, the expression profiles were analysed using reverse transcription/real-time quantitative PCR method from copepods subjected to different thermic and osmotic stresses in laboratory, and from copepods directly sampled into the natural population of the Seine Estuary (France) along a salinity gradient. Thermic shock (7.5°C, 22.5°C and 30°C during 90 min) significantly induced increases of transcript quantities ranged between 1.7- and 19.7-fold the levels observed in control conditions (15°C). Hypo- and hyper-osmotic shocks (salinities of 1 and 30 during 90 min) caused a 2-fold induction of Ea-hsp90A transcript level in comparison to controls (salinity of 15) whereas no significant change was measured for Ea-grp78. On the other hand, similar expression profiles were observed for the two transcripts after 72 h of exposition to salinities of 1 and 25 with a significant 2-fold induction observed for the lower salinity. To finish, strong expression inductions of both Ea-grp78 and Ea-hsp90A genes were observed in field copepods sampled at low salinity during the campaigns of June 2009 and May 2010. These results tend to show that the low salinity and the increase of temperature seem to have a synergic effect on stress condition of copepods.