Probing limits on spatial resolution using nonlinear optical effects and nonclassical light.

Using a simple optical setup to detect and characterize transmission gratings in the far field, we demonstrate that going beyond the diffraction limit is not possible using linear interaction of nonclassical illumination with the target grating. We also confirm that nonlinear optical interactions with the target grating, or with the optical medium around it, do allow improvement in resolution.

Optical pattern recognition by use of a segmented semiconductor optical amplifier.

A technique for high-speed, all-optical pattern recognition based on cross correlation in a segmented semiconductor optical amplifier (SSOA) is presented. A counterpropagating pump-probe setup is used to perform cross correlation of the spatial gain-loss pattern in the SSOA with the optical data pattern (pump), and the result is read out with a counterpropagating probe. Cross correlation of 4-bit patterns at 85 Gbits/s is experimentally demonstrated. Simulations show reasonable agreement with experimental measurements and are used to address scalability to higher bit rates and longer data patterns.

Inability of encapsulated Klebsiella pneumoniae to assemble functional type 1 fimbriae on their surface.

We screened phase variants of Klebsiella pneumoniae isolates for the expression of capsule and type 1 fimbriae and found that all of the 22 blood isolates were encapsulated and did not express type 1 fimbriae while 10 of 11 urinary tract isolates expressed type 1 fimbriae but were unencapsulated. Phase variants from selected isolates were found to be either unencapsulated and fimbriated or lacked both structures. Variants expressing both structures were not detected. Fimbrial subunits FimH and FimA were localized in the periplasmic space of the parent strain and on the surface of the unencapsulated variants. The results suggest that capsule formation impedes assembly of pre-formed fimbrial subunits on the bacterial surface.

Programmable high-bit-rate pattern generator with a segmented semiconductor optical amplifier.

We have demonstrated that spatial gain modulation in a segmented semiconductor optical amplifier can be converted to a temporal signal. A four-segment amplifier was used to generate digital return-to-zero patterns at 40 Gbits/s , and this technique should be readily scalable to more than 100 Gbits/s .

Inhibiting interspecies coaggregation of plaque bacteria with a cranberry juice constituent [published erratam appear in J Am Dent Assoc 1999 Jan;130(1):36 and 1999 Mar;130(3):332].

Dental plaque stability depends on bacterial adhesion to acquired pellicle, and on interspecies adhesion (or coaggregation). A high-molecular-weight cranberry constituent at 0.6 to 2.5 milligrams per milliliter reversed the coaggregation of 49 (58 percent) of 84 coaggregating bacterial pairs tested. It acted preferentially on pairs in which one or both members are gram-negative anaerobes frequently involved in periodontal diseases. Thus, the anticoaggregating cranberry constituent has the potential for altering the subgingival microbiota, resulting in conservative control of gingival and periodontal diseases. However, the high dextrose and fructose content of the commercially available cranberry juice makes it unsuitable for oral hygiene use, and the beneficial effect of the high-molecular-weight constituent requires animal and clinical studies.

Subnanosecond switching, plasma electrode pockels cell for high-contrast imaging with partially coherent light.

A high-contrast optical switch for imaging partially coherent light (~150 times the diffraction limit) requires that it have a large angular acceptance. We describe the development of a high-speed Pockels cell that uses a thin crystal to simultaneously achieve high-contrast (greater than 1800:1) and large-angular acceptance (greater than 7 mrad for a 5-mm aperture). A KD*P crystal was used in a longitudinal-mode configuration with plasma discharges forming low-resistance, high optical transmission electrodes to couple the switching voltage. Rise times of the switched optical pulse of the order of 500 ps were observed. Characterization of the device in the near and far fields was also performed.

Optical bit-pattern recognition by use of dynamic gratings in erbium-doped fiber.

We present a novel optical bit-pattern-recognition technique that uses erbium-doped fiber at room temperature. Counterpropagating beams write a patterned gain-depletion grating in pumped erbium-doped fiber. This grating, recorded in the erbium gain medium, can be used for correlation with other optical bit patterns. We have demonstrated correlation of arbitrary return-to-zero bit patterns of as many as 8 bits at 1 Gbit/s . Theory suggests that scaling to higher bit rates is feasible.

Invasive ability of C. jejuni/coli isolates from children with diarrhea and the effect of iron-regulated proteins.

The invasive ability of C. jejuni/coli strains isolated from children with diarrhea was studied using an in vitro HEp-2 cell invasion assay. The ratio between the number of intracellular bacteria and the number of bacteria in the inoculum was determined (invasion index). It was found that under anaerobic conditions, there was a significant decrease in the invasion index as compared to standard conditions (5% CO2). Of 11 strains tested, seven were determined as invasive on the basis of invasion indexes within the range of 0.0002-0.01. In a previous study [D. Schwartz et al., Zbl. Bakt. 280, 338-347 (1994)], it was found, that most of the C. jejuni/coli isolates tested produced an outer membrane protein when grown under conditions of iron depletion (IRP). The IRP were detected in eight of the nine strains tested in the present study (five invasive and three non-invasive strains). In one non-invasive strain, IRP was not detected. When kept under conditions of iron depletion, one of the invasive strains exhibited a significant increase in invasive capacity. The results suggest that iron depletion seems to stimulate the invasion capacity of C. jejuni/coli in vitro.

Technique for measurement of the Raman gain coefficient in optical fibers.

We demonstrate a simple, novel technique for measurement of the Raman gain spectrum in optical fibers. We measure the stimulated Raman scattered power generated by a square pulse as a function of its pulse width. Because of fiber dispersion, increasing the pulse width increases the interaction length of the pump and Stokes pulses and leads to a higher Stokes power. The dependence of growth rate of Stokes power on pulse width gives the Raman gain coefficient. Our technique does not require calibration against a standard or absolute measurement of the Stokes signal. We show that gain values obtained with this technique agree well with direct pump-probe measurements.

Nonopsonic phagocytosis of microorganisms.

Nonopsonic phagocytosis mediated by phagocyte receptors that recognize corresponding adhesins on microbial surfaces has attracted increasing interest as a potential host defense mechanism against extracellular pathogens and as a means of survival in the host for intracellular pathogens. Three types of nonopsonic phagocytosis involving carbohydrate-protein interactions (also termed lectinophagocytosis), protein-protein interactions, and hydrophobic interactions are discussed. A prominent receptor on phagocytic cells involved in recognizing pathogens belongs to the CD11/CD18 integrins. It mediates both opsonophagocytosis and nonopsonic phagocytosis and exhibits multiple specificity for different microbial adhesins. In other cases, similar specificity toward a microbial ligand (e.g. the Klebsiella pneumoniae capsule) is shared by dual molecules, one of which (e.g. the mannose-binding protein in serum) mediates opsonophagocytosis and the other (e.g. the macrophage mannose receptor) mediates nonopsonic phagocytosis of the microorganisms. In addition, we discuss how nonopsonic phagocytosis can trigger the phagocytes to release inflammatory agents and cause tissue injury. Further studies of the molecular mechanisms of nonopsonic phagocytosis, in particular those underlying the up-regulation of the phagocytic receptors by various agents, should lead to the development of new approaches for the prevention of infectious diseases.

Optical clock recovery from a data stream of an arbitrary bit rate by use of stimulated Brillouin scattering.

Using a fiber-optic stimulated-Brillouin-scattering amplifier as an active filter, we have demonstrated optical clock recovery from 5-Gbit/s return-to-zero-format optical data. Definite patterns and pseudorandom bit sequences were tested. This scheme requires no prior knowledge of the clock frequency and is well suited for operation at higher data rates.

Iron-regulated proteins in outer membranes of Campylobacter jejuni diarrhoea isolates and immune response to the proteins in patients.

The outer membrane protein (OMP) profiles from 8 Campylobacter jejuni and 5 Campylobacter coli fecal isolates grown under various conditions were compared by SDS-PAGE. The bacteria were grown under usual conditions, in iron-deficient medium (Dip) and on iron-supplemented medium (Fe). The OMP profiles of most bacterial strains grown under usual conditions, or in the Fe-supplemented medium, contained four major bands of approximately 31, 45, 63-66 and 97 kDa, and in addition, a number of minor bands. It was found that OMP from 10 of 13 strains tested and grown on iron deficient medium contained an intensive band of a protein in the molecular weight region of 76 kDa which was lacking in the OMP of bacteria grown in the presence of iron (iron-regulated protein). Sera from 11 children with C. jejuni infection analyzed by Western blot recognized the 76 kDa bands, in contrast to only one out of 10 control sera from healthy children. The Western-blot experiments demonstrated also various bands of other OMP components, both in OMP-Dip and OMP-Fe. The 45 kDa (porin protein) was recognized by all 11 serum samples from C. jejuni-infected patients and in 8 out of 10 control sera. The data suggested that the 76 kDa iron-regulated protein was expressed by bacteria during infection and it stimulated the immune response in children infected with C. jejuni.

Plasmid profiles and antimicrobial susceptibility of Campylobacter jejuni isolated from Israeli children with diarrhea.

Thirty Campylobacter jejuni (C. jejuni) strains isolated from stools of Israeli children with enteritis were tested for sensitivity to eight antimicrobial agents (MIC) and the presence of plasmids. It was found that all the isolates were sensitive to ciprofloxacin, ofloxacin, furazolidone and erythromycin. Of the 30 strains tested, 21 (70%) were found to be tetracycline-resistant, a relatively high resistance rate as compared with data from other countries and previous reports from Israel. Plasmids were detected in 17 out of 30 C. jejuni isolates (55.6%). A total of nine different plasmid profiles could be distinguished; six profiles were represented by one strain each. Of the 21 tetracycline-resistant strains, plasmids were found in 17 isolates (80%) carrying from 1-2 to 5 plasmids of various sizes. No plasmids were found in tetracycline-sensitive strains, with the exception of one isolate which contained a 24.4 MDa plasmid and was co-trimoxazole-resistant. Our studies indicate a relatively high percentage of tetracycline-resistant C. jejuni isolates in the Tel Aviv area. In 80% of these strains, various plasmid profiles were detected.

Photodamaged skin. Update on therapeutic management.

With baby boomers aging, the medical community is ushered into a new era of patient care, that of cosmetic maintenance and rejuvenation. The authors critically review pharmacologic treatments for preventing and treating photodamaged skin. Issues concerning sunscreens, tretinoin, silicone tissue augmentation, fat transplantation, collagen replacement therapy, and chemical exfoliation of the skin are addressed.