RESUMO
Introduction: This study aimed to evaluate the in vitro effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. Methods and Materials: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture (CFU/mL), the root canals were contaminated with E. faecalis biofilm, instrumented and then medicated according to the experimental groups. Three samples were collected from the root canals: after 21-days of contamination, immediately after the instrumentation and 14-days after the medication according to the experimental groups. The morphology of E. faecalis biofilm on the root canal walls and bacterial cells viability were assessed by means of SEM and CLSM, respectively. One-way ANOVA and Repeated Measures ANOVA tests were used to analyze the obtained data statistically. Results: CFU/mL analysis showed that CH, NAC and NAC+PDT promoted greater antibacterial activity with statistically significant difference compared to saline solution and PDT (P<0.0001). However, saline solution and PDT were statistically similar (P>0.07). Illustrative images by SEM confirmed partially the CFU/mL results. CLSM showed that all groups were effective eliminating E. faecalis except for the saline solution group. Conclusions: Based on this in vitro study NAC was bactericidal against E. faecalis biofilms regardless PDT stimulation, presenting similar antimicrobial activity to CH.
RESUMO
Introdução: a manutenção de um dente traumatizado na cavidade bucal depende de um tratamento emergencial correto, bem como de um longo e adequado período de acompanhamento clínico-imaginológico. Objetivo: relatar um caso clínico de avulsão e reimplante dentário do dente #11, em uma criança com 11 anos de idade, que permanecia na cavidade bucal há 13 anos. Relato de caso: foi feito reimplante dentário 45 minutos após a avulsão, tratamento endodôntico com 10 trocas mensais de medicação intracanal à base de hidróxido de cálcio, seguidas da obturação do canal radicular pela técnica de condensação lateral, e acompanhamento clínico e radiográfico. Resultados: após 13 anos de controle clínico e imaginológico, o dente #11 apresentava-se em função e esteticamente satisfatório. Conclusão: o incisivo central superior direito, avulsionado, reimplantado e anquilosado, apresentou um quadro evolutivo de reabsorção radicular por substituição, porém inativo até o último acompanhamento. Após tratamento endodôntico e acompanhamento clínico e imaginológico, o dente permaneceu na cavidade bucal em função, sem sintomatologia e esteticamente agradável após 13 anos do reimplante. (AU)
Introduction: The maintenance of a traumatized tooth in the oral cavity depends on a correct emergency treatment, as well as a long and adequate period of clinical-imaging follow-up. Objective: To report a clinical case of avulsion and replantation of tooth 11, in an 11-year-old child, who has been in the oral cavity for 13 years. Methods: tooth replantation 45 minutes after avulsion, endodontic treatment with 10 monthly exchanges of intracanal calcium hydroxide medication, followed by root canal filling using lateral condensation technique. Clinical and radiographic follow-up. Results: after 13 years of clinical and imaging control, tooth 11 presents aesthetically satisfactory function. Conclusion: The upper right central incisor, avulsed, replanted and ankylosed presented an evolutionary condition of root resorption by substitution, but inactive until the last follow-up. After endodontic treatment and clinical and imaging follow-up, the tooth remained in the oral cavity in function, without symptomatology and aesthetically pleasing after 13 years of replantation (AU).