Characteristics of Picea neoveitchii tree growth in mountain areas of central China: insights from isotopic compositions and satellite-derived indices.

Leaf nitrogen (N) status and stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) were used to study environmental factors that control mountain individuals of Picea neoveitchii trees, a coniferous species endemic and endangered in China. From May to September 2016, we carried out observations at four different altitude locations extending southeast of Daba Mountain in western Hubei Province. Needle-shaped leaf δ13C was positively correlated with needle N and C content calculated from the needle area (Narea and Carea content), needle δ15N, needle mass, and leaf mass per area (LMA), respectively. Needle δ15N was also positively correlated with monthly temperature and precipitation for the current month and last month. The seasonal normalised difference vegetation index (NDVI) was highest in June at the lowest altitude and August at the highest altitude. We found that N availability as an important driving factor for tree growth is controlled by surface soil temperature, while in summer, air temperatures above 23 °C exceed the physiological threshold of trees and limit the growth of trees. We concluded that the negative effect of higher temperature on tree growth is greater than the positive effect of higher nitrogen.

Antibiotic resistance gene profiling in response to antibiotic usage and environmental factors in the surface water and groundwater of Honghu Lake, China.

There is an increasing concern that aquaculture has been implicated in the formation of antibiotic resistance gene (ARG) reservoirs; however, little is known about the consequences of their presence in groundwater. In this study, 22 antibiotics, including four acetylated metabolites, and 27 ARGs were analyzed in fish pond water, surface water, and groundwater of the Honghu Lake in China. Correlations between conventional parameters, ionic composition, antibiotic concentration, and relative abundance of ARGs in water samples were analyzed. Among the three different sources of water, total antibiotic levels were the highest in fish pond water and the lowest in groundwater, with moderate levels in lake water. In surface water, sulfonamides and their metabolites accounted for the highest antibiotic content, whereas tetracyclines were the most frequently found in groundwater samples. Despite the near-undetectable levels of antibiotics in groundwater, the relative abundance of ARGs in groundwater samples was even higher than that in surface waters. The magnitude and extent of ARG migration are likely to be dependent on local antibiotic contamination levels as well as on the local environmental and hydrogeological conditions, with the class 1 integrons (intI1) being essential for the dissemination of such ARGs. The effects of environmental parameters such as antibiotics, dissolved oxygen, HCO3-, and pH on ARGs were highly significant, reflecting the potential impact of these factors on the abundance of ARGs. Our findings thus highlight the need for improved control of the spread of ARGs in and from aquaculture environments.

Identifying three ecological chemotypes of Xanthium strumarium glandular trichomes using a combined NMR and LC-MS method.

Xanthanolides, as the sesquiterpene lactones, are reportedly the major components for the pharmacological properties of X. strumarium L. species. Phytochemical studies indicated that the glandular structures on the surface of plant tissues would form the primary sites for the accumulation of this class of the compounds. As the interface between plants and their natural enemies, glandular trichomes may vary with respect to which of their chemicals are sequestered against different herbivores in different ecologies. However, to date, no data are available on the chemical characterisation of X. strumarium glandular cells. In this study, the trichome secretions of the X. strumarium species originating from nineteen unique areas across eleven provinces in China, were analysed by HPLC, LC-ESI-MS and NMR. For the first time three distinct chemotypes of X. strumarium glandular trichomes were discovered along with the qualitative and quantitative evaluations of their presence of xanthanolides; these were designated glandular cell Types I, II, and III, respectively. The main xanthanolides in Type I cells were 8-epi-xanthatin and xanthumin while no xanthatin was detected. Xanthatin, 8-epi-xanthatin, and xanthumin dominated in Type II cells with comparable levels of each being present. For Type III cells, significantly higher concentrations of 8-epi-xanthatin or xanthinosin (relative to xanthatin) were detected with xanthinosin only being observed in this type. Further research will focus on understanding the ecological and molecular mechanism causing these chemotype differences in X. strumarium glandular structures.

Determination of atractylenolide II in rat plasma by reversed-phase high-performance liquid chromatography.

A method for quantitative determination of atractylenolide II in rat plasma using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry was established. From a variety of compounds and solvents tested, atractylenolide III was selected as the internal standard (IS) and ethyl acetate was found to be the best solvent for extracting atractylenolide II from plasma samples. RP-HPLC analysis of the extracts was performed on an analytical column (DIKMA ODS, 150 x 4.6 mm; i.d., 5 microm) equipped with a security guard pre-column system. There was good linearity over the range 0.05-5.0 microg/mL (r > 0.99). The recoveries were more than 90.0% in plasma, and the intra- and inter-day coefficients of variation were less than 10.0% in all cases. The limit of detection (LOD) was 0.025 microg/mL and the lower limit of quantification (LLOQ) was 0.05 microg/mL. The RP-HPLC method was applied to quantitate atractylenolide II in rat plasma within 24 h in a pharmacokinetics study where experimental rats received a single dose of atractylenolide II (60 mg/kg).