RESUMO
In this 10-patient prospective pilot study, we show the feasibility of pragmatic direct ex vivo measurement of gadolinium retention from group II gadolinium-based contrasts agents (GBCAs) in young patients after routine tooth extraction. This noninvasive method may support future research attempting to understand the link between GBCA exposure and clinical outcomes.
Assuntos
Meios de Contraste , Gadolínio , Humanos , Gadolínio/farmacocinética , Feminino , Masculino , Extração Dentária , Adolescente , Imageamento por Ressonância Magnética/métodos , Espectrometria de Massas/métodos , CriançaRESUMO
Purpose: To assess the effectiveness of 38 percent silver diamine fluoride (SDF) in arresting cavitated caries lesions in young U.S. children. Methods: Children 12 to 71 months of age with severe early childhood caries participated in this phase three, multicenter, randomized, placebocontrolled trial. SDF was applied twice (at baseline and six months), and children were followed for eight months. A planned interim analysis of only the six-month primary outcome caries arrest data, for approximately half of the cohort (680 of 1,144 children), was conducted using a generalized estimating equation model, accounting for non-independence among carious lesions within a patient. Results: Five hundred ninety-nine of the 680 participants, with 1,413 lesions, completed the six-month exam. Lesions in the SDF group demonstrated 54 percent arrest versus 21 percent in the placebo (P<0.001). Conclusions: Silver diamine fluoride was effective at arresting active cavitated lesions in this population, leading to the early stop of the trial. Final analyses of all data and other outcomes are currently underway.
Assuntos
Suscetibilidade à Cárie Dentária , Cárie Dentária , Compostos de Amônio Quaternário , Criança , Humanos , Pré-Escolar , Cárie Dentária/prevenção & controle , Compostos de Prata/uso terapêutico , Fluoretos TópicosRESUMO
Purpose Non-surgical periodontal therapy (NSPT) is considered to be fundamental in the treatment of periodontal disease. Advanced area specific instruments were designed to increase the clinician's ability to effectively access root furcation areas during NSPT. The purpose of this study was to explore clinical dental hygienists' familiarity, utilization, and perceived efficacy of advanced instruments in root furcation areas during NSPT.Methods A randomized sample (n=3,500) of licensed dental hygienists in Michigan was invited to participate in a paper-based, mail survey. The 10-item instrument consisted of demographic, multiple choice, Likert scale, and open-ended questions. Descriptive and inferential statistics were used to analyze the data.Results A total of 1,156 surveys were returned; 858 met the inclusion criteria for a response rate of 24.5%. Respondents who graduated between 2010-2020 than those who graduated between 1990-1999 were more likely to utilize advanced instruments and those who graduated in 1989 or earlier (16.0% and 19.9% respectively). Respondents familiar with advanced instruments were more likely to use them in furcation areas during NSPT than those less familiar with the instruments (95% CI [18.1, 29.6], p<0.001). Respondents who perceived advanced instruments to be effective in furcation areas were more likely to utilize them (95% CI [1.0, 8.0], p<0.05) during NSPT. Most respondents indicated that they became familiar with advanced instruments during their dental hygiene education or through continuing education courses.Conclusion Familiarity with advanced instruments and perceived efficacy of these instruments for accessing root furcations increased the likelihood of clinical dental hygienists utilizing them during NSPT. Dental hygiene education and continuing education programs should continue to provide opportunities for students and practicing clinicians to learn NSPT instrumentation techniques utilizing advanced instruments designed for furcation access.
Assuntos
Higienistas Dentários , Educação Continuada , Humanos , Higienistas Dentários/educação , Michigan , Estudantes , Inquéritos e Questionários , Atitude do Pessoal de SaúdeRESUMO
BACKGROUND: An expert panel convened by the American Dental Association (ADA) Council on Scientific Affairs together with the ADA Science and Research Institute's program for Clinical and Translational Research conducted a systematic review and developed recommendations for the treatment of moderate and advanced cavitated caries lesions in patients with vital, nonendodontically treated primary and permanent teeth. TYPES OF STUDIES REVIEWED: The authors searched for systematic reviews comparing carious tissue removal (CTR) approaches in Ovid MEDLINE, Embase, Cochrane Database of Systematic Reviews, and Trip Medical Database. The authors also conducted a systematic search for randomized controlled trials comparing direct restorative materials in Ovid MEDLINE, Embase, Cochrane Central Register of Controlled Trials, ClinicalTrials.gov, and the World Health Organization International Clinical Trials Registry Platform. The authors used the Grading of Recommendations Assessment, Development, and Evaluation approach to assess the certainty of the evidence and formulate recommendations. RESULTS: The panel formulated 16 recommendations and good practice statements: 4 on CTR approaches specific to lesion depth and 12 on direct restorative materials specific to tooth location and surfaces involved. The panel conditionally recommended for the use of conservative CTR approaches, especially for advanced lesions. Although the panel conditionally recommended for the use of all direct restorative materials, they prioritized some materials over the use of others for certain clinical scenarios. PRACTICAL IMPLICATIONS: The evidence suggests that more conservative CTR approaches may decrease the risk of adverse effects. All included direct restorative materials may be effective in treating moderate and advanced caries lesions on vital, nonendodontically treated primary and permanent teeth.
Assuntos
American Dental Association , Cárie Dentária , Estados Unidos , Humanos , Suscetibilidade à Cárie Dentária , Revisões Sistemáticas como Assunto , Cárie Dentária/terapia , Bases de Dados Factuais , Materiais DentáriosRESUMO
BACKGROUND: The goal of restoring caries lesions is to protect the pulp, prevent progression of the disease process, and restore the form and function of the tooth. The purpose of this systematic review was to determine the effect of different direct restorative materials for treating cavitated caries lesions on anterior and posterior primary and permanent teeth. TYPE OF STUDIES REVIEWED: The authors included parallel and split-mouth randomized controlled trials comparing the effectiveness of direct restorative materials commercially available in the United States placed in vital, nonendodontically treated primary and permanent teeth. Pairs of reviewers independently conducted study selection, data extraction, and assessments of risk of bias and certainty of the evidence using the Grading of Recommendations Assessment, Development and Evaluation approach. The authors conducted pair-wise meta-analyses to summarize the evidence and calculated measures of association and their 95% CIs. RESULTS: Thirty-eight randomized controlled trials were eligible for analysis, which included data on Class I and Class II restorations on primary teeth and Class I, Class II, Class III, Class V, and root surface restorations on permanent teeth. Included studies assessed the effect of amalgam, resin composite, compomer, conventional glass ionomer cement, resin-modified glass isomer cement, and preformed metal crowns. Moderate to very low certainty evidence suggested varying levels of effectiveness across restorative materials. CONCLUSIONS AND PRACTICAL IMPLICATIONS: Owing to a relatively low event rate across various outcomes indicating restoration failure, there was limited evidence to support important differences between direct restorative materials used in practice.
Assuntos
Cárie Dentária , Restauração Dentária Permanente , Estados Unidos , Humanos , American Dental Association , Suscetibilidade à Cárie Dentária , Materiais Dentários/uso terapêutico , Cárie Dentária/prevenção & controle , Resinas Compostas , Dente Decíduo , Cimentos de Ionômeros de Vidro/uso terapêuticoRESUMO
Cariogenic biofilms produce strong acidic microenvironments, which is the primary cause of dental caries. Streptococcus mutans is a dominant species in cariogenic biofilms. Herein, we report a pH-responsive, charge-switching smart copolymer to selectively target and eradicate bacteria in cariogenic biofilms. To that end, the copolymer is designed to be activated in an acidic environment. The smart copolymer, Poly-1A, consists of ternary compositions of monomers with a cationic ethyl ammonium group, a carboxylic group, and a hydrophobic group in the side chains. The net charge of Poly-1A was charge neutral at neutral pH, but it switched to be cationic because the acidic carboxylate side chains were protonated and became neutral; however, the ammonium groups remained positive. Poly-1A with a net positive charge bound to the anionic surface of oral bacteria by electrostatic interactions and disrupted the bacterial membranes, causing bacterial death. Poly-1A reduced the cell viability of planktonic and biofilm S. mutans at pH 4.5, while it was not bactericidal at pH 7.4. Poly-1A did not reduce the cell viability of human gingival fibroblasts and periodontal ligament stem cells for a 1 h incubation.
Assuntos
Anti-Infecciosos , Cárie Dentária , Polímeros Responsivos a Estímulos , Humanos , Streptococcus mutans , Biofilmes , Polímeros/farmacologia , Polímeros/químicaRESUMO
Previous work has shown targeted fluorescent starch nanoparticles (TFSNs) can label the subsurface of carious lesions and assist dental professionals in the diagnostic process. In this study, we aimed to evaluate the potential of using artificial intelligence (AI) to detect and score carious lesions using ICDAS in combination with fluorescent imaging following application of TFSNs on teeth with a range of lesion severities, using ICDAS-labeled images as the reference standard. A total of 130 extracted human teeth with ICDAS scores from 0 to 6 were selected by a calibrated cariologist. Then, the same surface was imaged with a stereomicroscope under white light illumination, without visible fluorescence, and blue light illumination with an orange filter following application of the TFSNs. Both sets of images were labeled by another blinded ICDAS-calibrated cariologist to demarcate lesion position and severity. Convolutional neural networks, state-of-the-art models in imaging AI, were trained to determine the presence, location, ICDAS score (severity), and lesion surface porosity (as an indicator of activity) of carious lesions, and tested by 30 k-fold validation for white light, blue light, and the combined image sets. The best models showed high performance for the detection of carious lesions (sensitivity 80.26%, PPV 76.36%), potential for determining the severity via ICDAS scoring (accuracy 72%, SD 5.67%), and the detection of surface porosity as an indicator of the activity of the lesions (accuracy 90%, SD 7.00%). More broadly, the combination of targeted biopolymer nanoparticles with imaging AI is a promising combination of novel technologies that could be applied to many other applications.
Assuntos
Cárie Dentária , Nanopartículas , Humanos , Suscetibilidade à Cárie Dentária , Inteligência Artificial , Cárie Dentária/diagnóstico por imagem , Cárie Dentária/patologia , Redes Neurais de ComputaçãoRESUMO
OBJECTIVES: We have previously shown fluorescent cationic starch nanoparticles (FCSNs) penetrate enamel surface porosity of active carious lesions, potentially aiding their detection. Here, we evaluate the in vitro diagnostic accuracy of FCSNs in detecting occlusal caries compared to histologic reference standard. METHODS: 100 extracted human teeth were selected with sound (50), or either non-cavitated (25) or cavitated (25) lesions. A region of interest (ROI) on the occlusal surface was assessed for fluorescence by two independent examiners, after immersion in FCSN solution, water rinse, and illumination by dental curing lamp viewed through orange UV-filter glasses. ROIs were sectioned and evaluated by histology (Downer Criteria) as a gold standard for caries presence. Cohen's Kappa was determined for inter- and intra-examiner agreement, and sensitivity, specificity, and area under the curve of Receiver Operator Curves (ROCAUC) were calculated. The analysis was repeated for the subset of "early" lesions, defined as being limited to enamel. RESULTS: FCSN use resulted in substantial inter-user (k=0.74±0.07), and high intra-user agreement (k=0.80±0.06; 0.94±0.03, by examiner). Sensitivity, specificity and ROCAUC for FCSNs were 88.9%; 94.6%; 0.92±0.06 for all, and 76.9%, 94.6%, and 0.86±0.10 for early lesions. In post hoc analysis, sensitivity seemed to be greater with the FCSN than the expert visual exam, particularly for early lesions. CONCLUSIONS/CLINICAL SIGNIFICANCE: FCSNs are a reproducible and accurate novel technology for occlusal caries detection, with high sensitivity and specificity compared to histology. Future clinical validation is necessary. FCSNs can improve early caries detection and shift treatment towards non-invasive approaches, improving oral health.
Assuntos
Cárie Dentária , Nanopartículas , Cárie Dentária/diagnóstico , Suscetibilidade à Cárie Dentária , Fluorescência , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Amido , ÁguaRESUMO
OBJECTIVE: It is unclear whether tea infusions with or without sucrose supplementation alter oral biofilm development, so we evaluated the effect of unsweetened and sucrose-sweetened black and green tea infusions on in vitro saliva-derived biofilms. DESIGN: Biofilms were developed from human saliva for 20 h in cell-free 25% human saliva within static glass-bottom microplates. During biofilm development, biofilms were treated with either (i) unsweetened black tea, (ii) unsweetened green tea, (iii) 10% sucrose-sweetened black tea, (iv) 10% sucrose-sweetened green tea (v) deionized water (negative control), or (vi) 10% sucrose (positive control). Biofilms were incubated at 37 °C in 5% CO2. After 20 h of development, biofilms were imaged using a CLSM, and biofilm architecture and viability were evaluated. RESULTS: All the tea infusions reduced biofilm biomass and altered some other biofilm architectural outcomes (e.g., biofilm surface area) compared to the control groups. Statistically significant differences in biofilm biomass, number of objects, surface area, and convex-hull porosity were observed between biofilms treated with green and black tea. The addition of sugar to tea did not significantly modify the ability of tea to alter biofilm architecture. Only the treatment of biofilms with unsweetened black tea significantly reduced bacterial viability. CONCLUSIONS: While both teas reduced biofilm biomass and altered biofilm architecture, black tea had an enhanced effect that may relate to this tea's observed antimicrobial activity. The addition of sucrose to tea infusions did not appear to reduce the impact of either tea in modifying oral biofilm architecture.
Assuntos
Camellia sinensis , Chá , Biofilmes , Humanos , Saliva , Sacarose/farmacologiaRESUMO
Numerous in vitro biofilm model systems are available to study oral biofilms. Over the past several decades, increased understanding of oral biology and advances in technology have facilitated more accurate simulation of intraoral conditions and have allowed for the increased generalizability of in vitro oral biofilm studies. The integration of contemporary systems with confocal microscopy and 16S rRNA community profiling has enhanced the capabilities of in vitro biofilm model systems to quantify biofilm architecture and analyse microbial community composition. In this review, we describe several model systems relevant to modern in vitro oral biofilm studies: the constant depth film fermenter, Sorbarod perfusion system, drip-flow reactor, modified Robbins device, flowcells and microfluidic systems. We highlight how combining these systems with confocal microscopy and community composition analysis tools aids exploration of oral biofilm development under different conditions and in response to antimicrobial/anti-biofilm agents. The review closes with a discussion of future directions for the field of in vitro oral biofilm imaging and analysis.
Assuntos
Biofilmes , Microbiota , Antibacterianos , Reatores Biológicos , RNA Ribossômico 16SRESUMO
This laboratory study investigated the impact of tooth age on dental erosion susceptibility and preventive treatment efficacy. Extracted human premolars were selected and had their age estimated (â¼10-100 years old) using established dental forensic methods. Enamel and root dentin slabs were prepared, embedded in acrylic blocks, flattened, and polished. The specimens were randomly assigned to one of three treatments (n = 93): Sn+F (800 ppm Sn as SnCl2 and 250 ppm F as NaF, pH 4.5), NaF (250 ppm F, pH 4.5), or deionized water (DIW). Each specimen was subjected for 10 days to a daily cycling protocol consisting of six 5-min erosive challenges (0.3% citric acid, pH 2.6), six 60-min remineralization periods (artificial saliva), and three 2-min treatments with the test solutions. Surface loss (SL) was measured after 3, 5, and 10 days, using optical profilometry. Effects of tooth age, antierosive treatment, and time on SL were evaluated using linear mixed effects regression analysis. SL increased with age for all substrate-treatment-time combinations (p < 0.0001). Sn+F and NaF solutions significantly reduced SL compared to DIW, regardless of substrate, time, or age (p < 0.0001), with best results shown for Sn+F. Efficacy of Sn+F increased with tooth age on enamel, but tooth age did not affect the efficacy of NaF on enamel. For dentin, increased efficacy was observed with age after 5 (for Sn+F) and 10 days (for Sn+F and NaF). In conclusion, increase of tooth age rendered enamel and root dentin more susceptible to dental erosion. NaF preventive efficacy improved with tooth age for dentin, in advanced erosion simulation. Sn+F reduced enamel SL due to erosion regardless of tooth age.
Assuntos
Erosão Dentária , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Esmalte Dentário , Fluoretos/uso terapêutico , Humanos , Pessoa de Meia-Idade , Fluoreto de Sódio , Erosão Dentária/prevenção & controle , Resultado do Tratamento , Adulto JovemRESUMO
Despite evidence strongly supporting use of non-invasive or minimally invasive procedures in caries management, there is still a large gap between evidence-based recommendations and application of these concepts in practice, with the practice of dentistry still largely dominated by invasive procedures in the US. This paper describes efforts in education and clinical practice in the US in the last decade to promote evidence-based cariology strategies, which support a minimum intervention dentistry (MID) philosophy. These include, for example: a competency-based core cariology curriculum framework which has been developed and disseminated. National education accreditation standards supporting caries management are likely to soon be changed to support assessment of best evidence in cariology. There are several ongoing efforts by organised dentistry and other groups involving dental educators, researchers and clinical practitioners to promote cariology concepts in practice, such as the development of evidence-based clinical practice guidelines for caries management by the American Dental Association. Within each of these strategies there are challenges, but also opportunities to expand the implementation of MID in the US, which create optimism for future improvements over time.
Assuntos
Cárie Dentária , Educação em Odontologia , Currículo , Cárie Dentária/prevenção & controle , Odontologia , Humanos , Estados UnidosRESUMO
OBJECTIVES: This in vitro study explored quantitative outcome measures as clinical indicators of simulated occlusal tooth wear progression. METHODS: Ten sound, extracted human premolars were selected and submitted to occlusal tooth wear simulation in 0.5-mm steps (0/0.5/1.0/1.5â¯mm). At each step, enamel thickness on the buccal cusp tips was evaluated using cross-polarization optical coherence tomography (CP-OCT) and micro-computed tomography (µ-CT). The occlusal surface of each premolar was also scanned at each step using a 3D digital intraoral scanner, followed by morphological characterization using standard topography attributes (Slope, Relief, RFI, OPCr). Repeated measures ANOVA assessed differences in simulated wear levels for the µ-CT and CP-OCT data as well as the topography values. Correlations were also calculated between the µ-CT/CP-OCT and topography data. RESULTS: Significant differences were observed for enamel thickness at each simulation wear stage, for both CP-OCT (pâ¯<â¯0.001) and µ-CT (pâ¯<â¯0.001), with good agreement between methods (intraclass correlation: 0.89). For topography analysis, as wear increased, the average Slope, RFI, and Relief values decreased, and average OPCr values increased, with more significant differences shown for Slope. Slope showed significant (pâ¯<â¯0.05) positive correlations with CP-OCT. OPCr showed significant negative correlations with µ-CT, and CP-OCT (pâ¯<â¯0.05). RFI and Relief were not correlated with either µ-CT or CP-OCT (pâ¯>â¯0.05). CONCLUSIONS: Our findings suggest the potential of PS-OCT for measuring enamel thickness changes in the cusp tips of the occlusal surface. Similarly, conventional intraoral scanners can serve effectively for monitoring overall tooth wear when combined with dental topographic analyses of resultant point clouds. CLINICAL SIGNIFICANCE: CP-OCT measures of enamel thickness and dental 3D topographic attributes showed potential as objective outcomes for the clinical monitoring of occlusal tooth wear. Their combination provided a comprehensive understanding of the tooth wear development process.
Assuntos
Atrito Dentário , Desgaste dos Dentes , Esmalte Dentário/diagnóstico por imagem , Humanos , Avaliação de Resultados em Cuidados de Saúde , Tomografia de Coerência Óptica , Desgaste dos Dentes/diagnóstico por imagem , Microtomografia por Raio-XRESUMO
OBJECTIVES: Enamel thickness determination by Cross-Polarization Optical Coherence Tomography (CP-OCT) is a promising approach for quantitative monitoring of tooth wear progression. This study evaluated the ability of CP-OCT to quantify the thickness of natural enamel before, during and after tooth wear simulation. MATERIALS AND METHODS: Natural, unpolished human dental enamel slabs were submitted to five wear stages (Wear 1: to level the surfaces; Wear 2 to Wear 5: 0.05±0.02mm reduction each) simulated by an automatic grinding/polishing machine. Enamel thickness was evaluated with CP-OCT and a gold-standard method (micro-CT) at baseline and after every wear stage. Data were analyzed using ANOVA with pairwise comparisons for wear stages' impact on the thickness and wear depth measurements. The inter-method agreement was analyzed using intra-class correlation coefficients, the difference between means, and Bland-Altman plots. RESULTS: Enamel thickness measurements (mean±standard error, in mm) with natural (1.40±0.05) and worn surfaces (1.08±0.02) by CP-OCT did not differ significantly from those measured by micro-CT (natural=1.39±0.05; worn=1.09±0.02; p-values=0.30 and 0.39, respectively). CP-OCT and micro-CT showed excellent agreement on natural (ICC=0.98) and worn surfaces (ICC=0.98) enamel thickness measurements. Among and between wear stages, there were significant differences in enamel thickness and wear depth measurements for both methods (p-value <0.0001 for all). Both methods yielded similar measurements' mean (0.14±0.01; p-value=0.87) and were in good agreement (ICC=0.77) for wear depth estimation. SIGNIFICANCE: CP-OCT allows accurate measurement of enamel thickness on natural tooth surfaces. Enamel thickness measurement by CP-OCT allows quantitative monitoring of enamel thickness changes and wear depth following progressive wear.
Assuntos
Desgaste dos Dentes , Dente , Esmalte Dentário , Humanos , Tomografia de Coerência Óptica , Microtomografia por Raio-XRESUMO
OBJECTIVES: Lead (Pb) exposure is associated with dental caries. Whether Pb affects tooth microhardness, is unclear. Our objective was to assess whether Pb concentration is associated with microhardness. METHODS: Exfoliated primary teeth were collected from 46 volunteers. Teeth were sectioned, one half of each tooth was tested for enamel Knoop microhardness. The remaining half was digested and Pb measured using an inductively coupled plasma-mass spectrometer. RESULTS: The correlations between Pb levels and microhardness were very low, and were not statistically significant at p < 0.05. CONCLUSIONS: Previous exposure to high levels of Pb was not associated with decreased tooth microhardness. CLINICAL SIGNIFICANCE: This study assessed whether Pb in deciduous teeth is associated with tooth microhardness. As this was not the case, further studies are needed to identify the mechanisms behind the association between lead exposure and tooth decay.
RESUMO
Biofilm model systems are used to study biofilm growth and predict the effects of anti-biofilm interventions within the human oral cavity. Many in vitro biofilm model systems use a confocal laser scanning microscope (CLSM) in conjunction with image analysis tools to study biofilms. The aim of this study was to evaluate an in-house developed image analysis software program that we call BAIT (Biofilm Architecture Inference Tool) to quantify the architecture of oral multi-species biofilms following anti-biofilm interventions using a microfluidic biofilm system. Differences in architecture were compared between untreated biofilms and those treated with water (negative control), sodium gluconate ('placebo') or stannous fluoride (SnF2). The microfluidic system was inoculated with pooled human saliva and biofilms were developed over 22 h in filter-sterilized 25â% pooled human saliva. During this period, biofilms were treated with water, sodium gluconate, or SnF2 (1000, 3439 or 10â000 p.p.m. Sn2+) 8 and 18 h post-inoculation. After 22 h of growth, biofilms were stained with LIVE/DEAD stain, and imaged by CLSM. BAIT was used to calculate biofilm biovolume, total number of objects, surface area, fluffiness, connectivity, convex hull porosity and viability. Image analysis showed oral biofilm architecture was significantly altered by 3439 and 10â000 p.p.m. Sn2+ treatment regimens, resulting in decreased biovolume, surface area, number of objects and connectivity, while fluffiness increased (P<0.01). In conclusion, BAIT was shown to be able to measure the changes in biofilm architecture and detects possible antimicrobial and anti-biofilm effects of candidate agents.
Assuntos
Biofilmes , Processamento de Imagem Assistida por Computador/métodos , Boca/microbiologia , Software , Algoritmos , Antibacterianos/farmacologia , Bactérias/classificação , Bactérias/efeitos dos fármacos , Bactérias/isolamento & purificação , Fenômenos Fisiológicos Bacterianos , Técnicas Bacteriológicas/instrumentação , Técnicas Bacteriológicas/métodos , Biofilmes/efeitos dos fármacos , Humanos , Processamento de Imagem Assistida por Computador/instrumentação , Viabilidade Microbiana/efeitos dos fármacos , Saliva/microbiologia , Fluoretos de Estanho/farmacologiaRESUMO
OBJECTIVES: Optical Coherence tomography (OCT) is a promising clinical imaging technology for quantitative and objective assessment of dental erosion. We aimed to determine the influence of enamel surface roughness and demineralization severity (by erosive challenge) on dental surface loss measurements by cross-polarization OCT (CP-OCT). MATERIALS AND METHODS: Human enamel specimens were prepared with three surface roughness levels (very rough, rough and polished; n = 10 each). They were evaluated using CP-OCT and optical profilometry (gold standard) at baseline, and after 1, 2, 4, 6, 8, 16 and 24 h of erosion demineralization. The effects of roughness and demineralization on enamel loss were analyzed using ANOVA (alpha = 0.05). Intraclass correlation coefficients (ICCs) and Bland-Altman plots were used to evaluate inter-method agreement and intra-examiner repeatability. RESULTS: CP-OCT surface loss measurements did not significantly differ with the changes in enamel surface roughness (p = 0.27). Among demineralization severities, CP-OCT surface loss measurements at 1, 2, 4 and 8 h did not differ among each other, but they showed significantly lower enamel loss than 16 and 24 h; 6 and 16 h were significantly lower than 24 h (p < 0.05). Overall, CP-OCT and optical profilometry measurements did not differ (p = 0.73); however, ICC was relatively low (ICC = 0.34). Enamel loss estimation by CP-OCT presented an error of approximately ± 150 µm compared to profilometry. Intra-examiner repeatability with CP-OCT was excellent (ICC = 0.98). CONCLUSIONS: Enamel roughness did not affect CP-OCT measurements. The estimated error of CP-OCT measurements limited the appropriate assessment of enamel erosion surface loss, in the magnitude simulated in this study. CLINICAL RELEVANCE: Enamel thickness measurement by CP-OCT presents potential as an objective method for monitoring dental erosion lesions; however, its use may be limited for the assessment of the initial stages of enamel surface loss by erosion.
Assuntos
Desmineralização do Dente , Erosão Dentária , Esmalte Dentário , Humanos , Tomografia de Coerência ÓpticaRESUMO
OBJECTIVES: To evaluate the efficacy of different bleaching systems on artificially created stained-remineralized caries lesions; and to assess the susceptibility of the bleached lesions to further demineralization. METHODS: Human enamel specimens were sectioned, polished, demineralized, and randomly divided into six groups (n = 21) to create stained-remineralized lesions, either non-metallic (non-Met: G1, G2 and G3) or metallic (Met: G4, G5 and G6). G1 and G4 received no bleaching treatment, while G2 and G5 were treated with 15% carbamide peroxide (at-home bleaching protocol; 4 h/d×7), and G3 and G6 with 40% hydrogen peroxide (in-office bleaching protocol; 20min × 3). Susceptibility to further demineralization was tested after bleaching treatment. Lesion mineral loss and depth were measured by transversal microradiography, and color change by spectrophotometry. Outcomes were analyzed using ANOVA models followed by Fisher's PLSD tests (α = 0.05). RESULTS: Metallic-stained lesions were significantly darker (all p < 0.001) and more resistant to bleaching (p < 0.005) than non-Met ones. For both stain types, the at-home bleaching protocol was more effective than the in-office (p < 0.005); however, it also increased the lesion susceptibility to demineralization (p < 0.05) [ΔΔZ mean ± SD ranging from 205 ± 73 to 313 ± 188 (at home) vs. 132 ± 45 to 206 ± 98 (in office); p < 0.05]. After bleaching, non-Met lesions were significantly more susceptible to demineralization (p < 0.05), with the ΔΔZ ranging from 206 ± 98 to 313 ± 188 compared to Met lesions ranging from 132 ± 45 to 205 ± 73. CONCLUSIONS: At-home bleaching protocol presented greater bleaching efficacy compared to in-office bleaching protocol. After bleaching, metallic-stained lesions were more resistant to subsequent demineralization compared to non-metallic stained lesions. CLINICAL SIGNIFICANCE: Bleaching stained-arrested caries lesions may improve aesthetics but also increase susceptibility to demineralization, depending on the type of stain involved and bleaching system used.
Assuntos
Cárie Dentária , Clareadores Dentários , Clareamento Dental , Desmineralização do Dente , Peróxido de Carbamida , Esmalte Dentário , Humanos , Peróxido de HidrogênioRESUMO
Toothpastes are the most universally accepted form of fluoride delivery for caries prevention. To provide anti-caries benefits, they must be able to release fluoride during the time of tooth brushing or post brushing into the oral cavity. However, there is no standard accepted procedure to measure how much fluoride in a toothpaste may be (bio) available for release. The European Organization for Caries Research proposed and supported a workshop with experts in fluoride analysis in toothpastes and representatives from industry. The objective of the workshop was to discuss issues surrounding fluoride analysis in toothpaste and reach consensus on terminology and best practices, wherever the available evidence allowed it. Participants received a background paper and heard presentations followed by structured discussion to define the problem. The group also reviewed evidence on the validity, reliability and feasibility of each technique (namely chromatography and fluoride electroanalysis) and discussed their strengths and limitations. Participants were able to reach a consensus on terminology and were also able to identify and summarize the advantages and disadvantages of each technique. However, they agreed that most currently available methods were developed for regulatory agencies several decades ago, utilizing the best available data from clinical trials then, but require to be updated. They also agreed that although significant advances to our understanding of the mechanism of action of fluoride in toothpaste have been achieved over the past 4 decades, this clearly is an extraordinarily complex subject and more work remains to be done.
Assuntos
Cárie Dentária , Cremes Dentais , Cariostáticos , Fluoretos , Humanos , Reprodutibilidade dos TestesRESUMO
OBJECTIVE: Non-invasive esthetic treatment options for stained arrested caries lesions have not been explored. This study aimed to develop laboratory models to create stained-remineralized caries-like lesions (s-RCLs) and to test the efficacy of bleaching on their esthetic treatment. MATERIALS AND METHODS: One hundred twelve enamel/dentin specimens were prepared from human molars, embedded, and had their color measured spectrophotometrically at baseline and after demineralization. They were randomly divided into four groups (n = 14) based on the staining/remineralization protocols for a total of 5 days: G1, no staining/no remineralization; G2, no staining/remineralization in artificial saliva (AS); G3, non-metallic staining/remineralization with sodium fluoride/AS; and G4, metallic staining/remineralization with silver diamine fluoride/AS. The lesion mineral loss (ΔZ) and depth (L) were measured using transverse microradiography along with color change (ΔE). Specimens were bleached and color was re-evaluated. Data were analyzed using ANOVA models followed by Fisher's PLSD tests (α = 0.05). RESULTS: s-RCLs in G4 were significantly (p < 0.001) darker than G3, G2, and G1 regardless of substrate type and condition. s-RCLs in G2, G3, and G4 showed significantly lower ΔZ and L than G1 (all p < 0.001), confirming occurrence of remineralization. G4 exhibited significantly lower ΔZ and L compared to G2 (p < 0.001). Bleaching was more effective in non-metallic than in metallic stained lesions regardless of substrate type (p < 0.001). CONCLUSION: The proposed models created distinct s-RCLs. Non-metallic s-RCLs were lighter and more responsive to bleaching compared to metallic s-RCLs. CLINICAL RELEVANCE: The developed experimental models allow the further investigation of the efficacy and safety of different clinical strategies for the esthetic management of s-RCLs.