[Cloning the Staphylococcus aureus enterotoxin B gene, obtained by polymerase chain reaction, and its expression in Escherichia coli cells]. / Klonirovanie gena énterotoksina B Staphylococcus aureus, poluchennogo metodom polimeraznoi tsepnoi reaktsii, i ego ékspressiia v kletkakh Escherichia coli.

To determine the Staphylococcus aureus enterotoxigenicity, we have developed an approach based on polymerase chain reaction (PCR). Using this method several S. aureus strains have been screened for the presence of the enterotoxin B gene. A DNA fragment of the selected strain (FRI 722H) containing enterotoxin B gene has been obtained by the PCR method and cloned in the pUC19 vector. It is shown that enterotoxin B with the leader peptide forms insoluble complexes in E. coli cells, whereas the mature toxin is present in cytoplasmic fraction in a soluble form. The recombinant toxin made up for 1.7% of the total cellular protein in E. coli JM 109 cells.

[The use of monoclonal antibodies for determining the transferrin content of the urine]. / Ispol'zovanie monoklonal'nykh antitel dlia opredeleniia soderzhaniia transferrina v moche.

The authors used monoclonal antibodies to human transferrin for the development of a high-sensitive test system for the assessment of transferrin levels in urine with enzyme immunoassay. The conditions of the immunoassay performance were optimized. The sensitivity of the test system enabled the authors to define the levels of transferrin up to 1 ng/ml. Tests for the detection of transferrin in patients with chronic pyelonephritis and chronic renal failure were performed. The designed test system permitted transferrin to be revealed in the urine of those patients who had negative results during a radial immunodiffusion. The authors demonstrated the possibility of using the developed monoclonal antibodies in laser nephelometry for the detection of blood and urinary transferrin levels.

[The characteristics of proteinuria during intensive physical loading]. / Osobennosti proteinurii pri intensivnykh fizicheskikh nagruzakh.

Intensive physical exercise result in certain changes in the body, kidneys in particular. A total of 23 highly qualified sportsmen were investigated for plasma proteins in the urine before and after the graded bicycle ergometry. The impairment of renal reabsorption due to physical load was evidenced by significant amounts of albumin in the afterload urinalyses of all the examinees, and the appearance of IgG in urinalysis of 6 sportsmen in comparison to the urinalysis before the exercise which reported insignificant levels of albumin in 6 subjects only.

[The differential diagnosis of allergic prostatitis]. / Differentsial'naia diagnostika allergicheskogo prostatita.

The leading role of allergic components was established in some rare patterns of prostatitis. To develop the methods applicable for differential diagnosis of allergic prostatitis, the authors followed up 60 patients aged from 15 to 53 years who suffered from chronic prostatitis and 10 normal controls. The patients with bacterial disease, abacterial prostatitis with concurrent allergic components or subjects with allergic prostatitis were enrolled in the study. Immunoassay was an essential part in the diagnosis of allergic prostatitis. The study revealed a dramatic inhibition of ejaculate gamma-glutamine transferase in those who suffered from an abacterial pattern with allergic component and almost three-fold in those with allergic prostatitis. Certain though insignificant decrease in the ejaculate gamma-glutamine transferase was documented in patients with bacterial prostatitis. Investigations of ejaculate IgE revealed quite an opposite picture: 10-fold increase in IgE levels in the ejaculate of patients with allergic prostatitis as compared with other groups, controls in their number. Besides, patients with allergic prostatitis had three-fold higher IgE in the ejaculate than in the blood serum. The aforementioned phenomenon failed to be observed in patients with other patterns of prostatitis. Decreased ejaculate gamma-glutamine transferase and increased IgE can be regarded as criteria for differential diagnosis of allergic prostatitis.

[The early stages of chronic kidney failure in children with congenital obstructive uropathies and results of surgical treatment]. / Rannie stadii khronicheskoi pochechnoi nedostatochnosti u detei s vrozhdennymi obstruktivnymi uropatiiami i rezul'taty operativnogo lecheniia.

Ninety-six children, aged 4 to 14, with congenital obstructive uropathies were subjected to 131I-hippuran renography, followed by mathematical processing of renographic curves, the measurement of blood mean molecular levels, and blood and urinary immunochemical tests before, and 1 month as well as 1 year after surgery. Intraoperative renal biopsy was taken from 20 patients with unilateral hydronephrosis. Latent chronic renal failure (CRF) was identified where a deficiency of total renal clearance of 131I-hippuran (20 to 56%) was combined with a rise in blood mean molecules from 0.3 to 0.41 conventional units at 254 nm. Latent CRF was detected in 40 of 64 children with unilateral uropathy and in all 32 patients with bilateral uropathy. In patients with unilateral hydronephrosis, the presence of CRF was unrelated to the morphological pattern of pyelonephritis in the affected kidney. Children with latent CRF showed high levels of urinary IgG and albumin and blood mean molecules. One year after the operation, renal function improved in patients whose contralateral kidney had no secretory deficiency. One year after surgery, renal reabsorption mechanisms tended to recover in CRF-free patients only. The clinical pattern of latent CRF and its elimination following surgery were unrelated to roentgenologic markedness of hydronephrosis and VUR, but were dependent on the recovery of compensatory mechanisms in the contralateral kidney. By the end of the first postoperative year, latent CRF was diagnosed in 31% of children with unilateral hydronephrosis (as compared to the preoperative 68%), 43% (vs. the preoperative 60%) of children with unilateral VUR, and in 44% (vs. the preoperative 50%) of patients with unilateral neuromuscular ureteral dysplasia.(ABSTRACT TRUNCATED AT 250 WORDS)