RESUMO
BACKGROUND: Immunoglobulin A dominant postinfectious glomerulonephritis (IgA PIGN) is a unique medical entity that is rare in the paediatric population. It usually presents with severe renal failure, heavy proteinuria, hypertension, and hypocomplementemia and frequently has an unfavourable prognosis. IgA PIGN generally occurs in association with staphylococcal infections and diabetes mellitus in adult patients. Other pathogens include Escherichia coli and Streptococcus sp. Immunofluorescence studies of kidney biopsy samples show IgA as dominant or codominant antibody. CASE PRESENTATION: We encountered a 3-year-old girl with IgA PIGN presenting with acute renal failure, oedema, hypertension, and heavy proteinuria of 7955 mg/g creatinine. Renal biopsy specimens showed diffuse glomerular endocapillary hypercellularity with prominent neutrophil and monocyte infiltration on light microscopy. Strong deposits of IgA and C3 were observed along the glomerular basement membranes and the mesangium by immunofluorescence microscopy, and electron microscopy revealed the presence of subepithelial humps. The patient was managed with steroid (and probatory antibiotic) therapy and is now undergoing follow-up, with a significant improvement 6 months after the initial presentation (glomerular filtration rate (GFR) and cystatin C clearance rate of 165 ml/min/1.73m2 and 106 ml/min/1.73m2, respectively). No signs of bacterial infection were detectable. CONCLUSION: This variant of IgA PIGN must be distinguished from other clinical entities, especially IgA nephropathy (mesangial IgA deposits) and postinfectious glomerulonephritis (C3, IgG and occasional IgM capillary loop deposits with or without mesangial distribution), since patients with IgA PIGN may require steroid treatment in addition to antibiotic therapy. Differential diagnosis should also include C3 glomerulopathy. IgA PIGN is a recently identified disease entity that generally manifests in adult patients with both IgA and C3 mesangial and glomerular capillary wall deposits. We present a biopsy-proven case of IgA PIGN that manifested in a patient at an exceptionally young age and that has had a good clinical outcome. To the best of our knowledge, this is the youngest IgA PIGN patient reported thus far.
Assuntos
Glomerulonefrite por IGA , Glomerulonefrite , Hipertensão , Infecções Estafilocócicas , Adulto , Antibacterianos/uso terapêutico , Biópsia , Criança , Pré-Escolar , Creatinina , Cistatina C , Feminino , Glomerulonefrite/complicações , Glomerulonefrite/tratamento farmacológico , Glomerulonefrite/microbiologia , Glomerulonefrite por IGA/complicações , Glomerulonefrite por IGA/diagnóstico , Glomerulonefrite por IGA/tratamento farmacológico , Humanos , Hipertensão/complicações , Imunoglobulina A , Imunoglobulina G , Imunoglobulina M , Proteinúria/complicações , Infecções Estafilocócicas/complicaçõesRESUMO
BACKGROUND: Glucosylceramide synthase (GCS; gene: UDP-glucose:ceramide glucosyltransferase (Ugcg))-derived gangliosides comprise a specific class of lipids in the plasma membrane that modulate the activity of transmembrane receptors. GCS deletion in hypothalamic arcuate nucleus (Arc) neurons leads to prominent obesity. However, it has not yet been studied how ganglioside depletion affects individual Arc neuronal subpopulations. The current study investigates the effects of GCS deletion specifically in anorexigenic pro-opiomelanocortin (POMC) neurons. Additionally, we investigate insulin receptor (IR) signaling and phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) binding to ATP-dependent K+ (KATP) channels of GCS-deficient POMC neurons. MATERIALS AND METHODS: We generated Ugcgf/f-Pomc-Cre mice with ganglioside deficiency in POMC neurons. Moreover, the CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas9 technology was used to inhibit GCS-dependent ganglioside biosynthesis in cultured mouse POMC neurons, yielding UgcgΔ-mHypoA-POMC cells that were used to study mechanistic aspects in further detail. Proximity ligation assays (PLAs) visualized interactions between gangliosides, IR, and KATP channel subunit sulfonylurea receptor-1 (SUR-1), as well as intracellular IR substrate 2 (IRS-2) phosphorylation and PIP3. RESULTS: Chow-fed Ugcgf/f-Pomc-Cre mice showed a moderate but significant increase in body weight gain and they failed to display an increase of anorexigenic neuropeptide expression during the fasting-to-re-feeding transition. IR, IRS-2, p85, and overall insulin-evoked IR and IRS-2 phosphorylation were elevated in ganglioside-depleted UgcgΔ-mHypoA-POMC neurons. A PLA demonstrated that more insulin-evoked complex formation occurred between PIP3 and SUR-1 in ganglioside-deficient POMC neurons in vitro and in vivo. CONCLUSION: Our work suggests that GCS deletion in POMC neurons promotes body weight gain. Gangliosides are required for an appropriate adaptation of anorexigenic neuropeptide expression in the Arc during the fasting-to-re-feeding transition. Moreover, gangliosides might modulate KATP channel activity by restraining PIP3 binding to the KATP channel subunit SUR-1. Increased PIP3/SUR-1 interactions in ganglioside-deficient neurons could in turn potentially lead to electrical silencing. This work highlights that gangliosides in POMC neurons of the hypothalamic Arc are important regulators of body weight.
Assuntos
Gangliosídeos , Glucosiltransferases , Hipotálamo , Pró-Opiomelanocortina/metabolismo , Animais , Gangliosídeos/deficiência , Gangliosídeos/genética , Gangliosídeos/metabolismo , Deleção de Genes , Glucosiltransferases/genética , Glucosiltransferases/metabolismo , Hipotálamo/citologia , Hipotálamo/metabolismo , Masculino , Camundongos , Camundongos Transgênicos , Transdução de Sinais/genéticaRESUMO
Treatment with tumor necrosis factor alpha (TNF-α) inhibitors is a well-established therapeutic strategy for various autoimmune diseases. However, little is known about renal complications and possible causality of renal injury due to this treatment. The following case of a patient with psoriasis demonstrates the difficulties in classifying renal complications of anti-TNF-α therapy versus kidney involvement caused by the underlying disease.
Assuntos
Adalimumab/uso terapêutico , Psoríase/tratamento farmacológico , Insuficiência Renal/induzido quimicamente , Fator de Necrose Tumoral alfa/uso terapêutico , Ustekinumab/uso terapêutico , Glomerulonefrite por IGA , Humanos , Infliximab , Masculino , Pessoa de Meia-Idade , Fator de Necrose Tumoral alfa/antagonistas & inibidoresRESUMO
Resistance towards the drug 5-fluorouracil (5-FU) is a key challenge in the adjuvant chemotherapy of colorectal cancer (CRC), and novel targeted approaches are required to improve the therapeutic outcome. Necroptosis is a recently discovered form of programmed cell death, which depends on receptor interacting protein 1 (RIP1) and particularly occurs under caspase-deficient conditions. The targeted induction of necroptosis represents a promising strategy to overcome apoptosis resistance in cancer. The aim of this study was to systematically explore the usage of pan-caspase inhibitors to sensitize resistant CRC cells for 5-FU. We found that pan-caspase inhibitors facilitated 5-FU-induced necroptosis, which was mediated by autocrine secretion of tumor necrosis factor α (TNF-α). TNF-α production was driven by nuclear factor κB (NF-κB) and required RIP1 kinase. In vivo xenograft experiments showed that the novel pan-caspase inhibitor IDN-7314 in combination with 5-FU synergistically blocked tumor growth. Ex vivo experiments with fresh human CRC tissue specimens further indicated that a subgroup of patients could benefit from combinatory treatment. Thereby, elevated levels of secreted TNF-α and expression of components of the necroptotic pathway might help to predict the sensitivity to pro-necroptotic therapies. Together, our results shed new light on the molecular regulation of necroptosis by NF-κB and RIP1. Moreover, we identify necroptotic cell death as an important effector mechanism of 5-FU-mediated anti-tumoral activity. On the basis of this study, we propose pan-caspase inhibitors as a novel approach in the adjuvant chemotherapy of CRC.
Assuntos
Apoptose/efeitos dos fármacos , Caspases/metabolismo , Neoplasias do Colo/tratamento farmacológico , Fluoruracila/farmacologia , NF-kappa B/metabolismo , Proteína Serina-Treonina Quinases de Interação com Receptores/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Animais , Antimetabólitos Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Neoplasias do Colo/metabolismo , Neoplasias do Colo/ultraestrutura , Células HCT116 , Células HT29 , Humanos , Immunoblotting , Imuno-Histoquímica , Masculino , Camundongos Nus , Microscopia Eletrônica , NF-kappa B/genética , Necrose , Oligopeptídeos/farmacologia , Interferência de RNA , Proteína Serina-Treonina Quinases de Interação com Receptores/genética , Receptores Tipo I de Fatores de Necrose Tumoral/genética , Receptores Tipo I de Fatores de Necrose Tumoral/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Carga Tumoral/efeitos dos fármacos , Carga Tumoral/genética , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
Methylmalonic acidurias (MMAurias) are a group of inherited disorders in the catabolism of branched-chain amino acids, odd-chain fatty acids and cholesterol caused by complete or partial deficiency of methylmalonyl-CoA mutase (mut(0) and mut(-) subtype respectively) and by defects in the metabolism of its cofactor 5'-deoxyadenosylcobalamin (cblA, cblB or cblD variant 2 type). A long-term complication found in patients with mut(0) and cblB variant is chronic tubulointerstitial nephritis. The underlying pathomechanism has remained unknown. We established an in vitro model of tubular epithelial cells from patient urine (hTEC; 9 controls, 5 mut(0), 1 cblB). In all human tubular epithelial cell (hTEC) lines we found specific tubular markers (AQP1, UMOD, AQP2). Patient cells showed disturbance of energy metabolism in glycolysis, mitochondrial respiratory chain and Krebs cycle in concert with increased reactive oxygen species (ROS) formation. Electron micrographs indicated increased autophagosome production and endoplasmic reticulum stress, which was supported by positive acridine orange staining and elevated levels of LC3 II, P62 and pIRE1. Screening mTOR signaling revealed a release of inhibition of autophagy. Patient hTEC produced and secreted elevated amounts of the pro-inflammatory cytokine IL8, which was highly correlated with the acridine orange staining. Summarizing, hTEC of MMAuria patients are characterized by disturbed energy metabolism and ROS production that lead to increased autophagy and IL8 secretion.
Assuntos
Erros Inatos do Metabolismo dos Aminoácidos/patologia , Túbulos Renais Proximais/metabolismo , Túbulos Renais Proximais/ultraestrutura , Adolescente , Adulto , Erros Inatos do Metabolismo dos Aminoácidos/urina , Autofagia , Linhagem Celular , Linhagem Celular Transformada , Criança , Pré-Escolar , Metabolismo Energético , Células Epiteliais/patologia , Humanos , Lactente , Interleucina-8/metabolismo , Nefrite Intersticial/metabolismo , Nefrite Intersticial/patologia , Fenótipo , Acidemia Propiônica/patologia , Espécies Reativas de Oxigênio/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Urina/citologia , Adulto JovemRESUMO
Transforming growth factor-ß1 (TGF-ß1) is a multifunctional cytokine and critically involved in the progression of a variety of cancers. TGF-ß1 signaling can impair tumor development by its anti-proliferative and pro-apoptotic features. In contrast, it may actively promote tumor progression and cancer cell dissemination by inducing a gradual switch from epithelial towards mesenchymal-like cell features (EMT-like), including decreased intercellular adhesion. Here, we show that expression of the transcription factor Basonuclin-1 (Bnc1) modulates TGF-ß1-induced epithelial dedifferentiation of mammary epithelial cells. RNAi-mediated repression of Bnc1 resulted in enhanced intercellular adhesion and strongly impaired TGF-ß1-dependent sheet disintegration and cell scattering. In contrast, forced expression of Bnc1 modifies plasma membrane/cytoskeletal dynamics and seemingly interferes with the initiation of sustainable cell-cell contacts. Follow-up analyses revealed that Bnc1 affects the expression of numerous TGF-ß1-responsive genes including distinct EMT-related transcription factors, some of which modulate the expression of Bnc1 themselves. These results suggest that Bnc1 is part of a transcription factor network related to epithelial plasticity with reciprocal feedback-loop connections on which Smad-factors integrate TGF-ß1 signaling. Our study demonstrates that Bnc1 regulates epithelial plasticity of mammary epithelial cells and influences outcome of TGF-ß1 signaling.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Células Epiteliais/metabolismo , Glândulas Mamárias Humanas/metabolismo , Transdução de Sinais , Fatores de Transcrição/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Desdiferenciação Celular , Transformação Celular Neoplásica/metabolismo , Proteínas de Ligação a DNA/genética , Transição Epitelial-Mesenquimal , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Humanos , Glândulas Mamárias Humanas/patologia , Fatores de Transcrição/genética , Homeobox 1 de Ligação a E-box em Dedo de ZincoRESUMO
Only described in the last 10 years, IgG4-related disease is a fibroinflammatory disorder characterized by tumorous lesions with dense lymphoplasmacytic infiltration by IgG4-positive plasma cells and often elevated concentration of serum IgG4. In this paper, we present a male patient with this disease involving the lymph nodes and possibly the joints and kidneys. Infiltration of lymph node tissue with IgG4-positive plasma cells was demonstrated. The general condition of the patient improved considerably by immunosuppressive therapy.
Assuntos
Artrite/diagnóstico , Artrite/tratamento farmacológico , Imunoglobulina G/sangue , Imunossupressores/uso terapêutico , Paresia/diagnóstico , Paresia/tratamento farmacológico , Artrite/imunologia , Diagnóstico Diferencial , Humanos , Masculino , Pessoa de Meia-Idade , Paresia/imunologia , Síndrome , Resultado do TratamentoRESUMO
BACKGROUND AND OBJECTIVE: Renal disease is a common complication in HIV-infected patients. The causes and spectrum of kidney disease among these patients is extensive, including HIV-related and HIV unrelated causes. Our objective was to assess the changes in distribution of renal disease under antiretroviral therapy (ART). PATIENTS AND METHODS: Retrospective analysis of all patients from the Frankfurt HIV Cohort (FHC) who underwent renal biopsy because of chronic, progressive renal disease between 1989 and 2012. Two time periods were defined: 1989-2001 (early period) and 2000-2012 (late period). RESULTS: 69 HIV-infected patients, mostly Caucasian and male, underwent renal biopsy (early period: 22 patients, late period: 47 patients). During the total observation time immuncomplex-mediated glomerulonephritis (26.1 %), hypertensive (20.3 %) and diabetic nephropathy (20.3 %) were the most frequent causes of chronic renal disease. HIV-associated renal diseases were predominant in the first period, whereas hypertensive and diabetic kidney disease accounted for almost 50 % of cases diagnosed in the late period. Other types of renal disease frequently encountered during the late period include renal AA-amyloidosis and tenofovir-related kidney disease. CONCLUSION: The underlying pathology of renal disease in HIV-infected patients is highly variable and evolving. Since the introduction of HAART, renal disease not directly related to HIV has become the predominant cause, reflecting the growing burden of co-morbidities in this aging population.
Assuntos
Nefropatia Associada a AIDS/epidemiologia , Fármacos Anti-HIV/uso terapêutico , Nefropatia Associada a AIDS/diagnóstico , Nefropatia Associada a AIDS/tratamento farmacológico , Nefropatia Associada a AIDS/patologia , Adenina/efeitos adversos , Adenina/análogos & derivados , Adenina/uso terapêutico , Adulto , Amiloidose/diagnóstico , Amiloidose/epidemiologia , Amiloidose/patologia , Terapia Antirretroviral de Alta Atividade/efeitos adversos , Biópsia , Estudos de Coortes , Estudos Transversais , Nefropatias Diabéticas/diagnóstico , Nefropatias Diabéticas/epidemiologia , Nefropatias Diabéticas/patologia , Feminino , Seguimentos , Alemanha , Glomerulonefrite/diagnóstico , Glomerulonefrite/epidemiologia , Glomerulonefrite/patologia , Humanos , Hipertensão Renal/diagnóstico , Hipertensão Renal/epidemiologia , Hipertensão Renal/patologia , Doenças do Complexo Imune/diagnóstico , Doenças do Complexo Imune/epidemiologia , Doenças do Complexo Imune/patologia , Rim/patologia , Masculino , Pessoa de Meia-Idade , Nefrite/diagnóstico , Nefrite/epidemiologia , Nefrite/patologia , Organofosfonatos/efeitos adversos , Organofosfonatos/uso terapêutico , Estudos Retrospectivos , Proteína Amiloide A Sérica/metabolismo , TenofovirRESUMO
Chronic renal allograft damage (CAD) is manifested by a smoldering inflammatory process that leads to transplant glomerulopathy, diffuse interstitial fibrosis and tubular atrophy with loss of tubular structures. Using a Fischer 344 (RT1lvl) to Lewis (RT1l) rat renal allograft model, transcriptomic profiling and pathway mapping, we have previously shown that dynamic dysregulation of the Wnt signaling pathways may underlie progressive CAD. Retinoic acid, an important regulator of differentiation during vertebrate embryogenesis, can moderate the damage observed in this experimental model of CAD. We show here that subsets of the Hedgehog (Hh) and canonical Wnt signaling pathways are linked to the pathophysiology of progressive fibrosis, loss of cilia in epithelia and chronic dysfunction. Oral treatment with 13cis retinoic acid (13cRA) was found to selectively ameliorate the dysregulation of the Hh and canonical Wnt pathways associated with CAD, and lead to a general preservation of cilial structures. Interplay between these pathways helps explain the therapeutic effects of retinoic acid treatment in CAD, and suggests future targets for moderating chronic fibrosing organ damage.
Assuntos
Proteínas Hedgehog/metabolismo , Transdução de Sinais , Tretinoína/metabolismo , Proteínas Wnt/metabolismo , Animais , Ratos , Ratos Endogâmicos F344RESUMO
The Forkhead family of transcription factors comprises numerous members and is implicated in various cellular functions, including cell growth, apoptosis, migration, and differentiation. In this study, we identified the Forkhead factor FoxQ1 as increased in expression during TGF-ß1 induced changes in epithelial differentiation, suggesting functional roles of FoxQ1 for epithelial plasticity. The repression of FoxQ1 in mammary epithelial cells led to a change in cell morphology characterized by an increase in cell size, pronounced cell-cell contacts, and an increased expression of several junction proteins (e.g., E-cadherin). In addition, FoxQ1 knock-down cells revealed rearrangements in the actin-cytoskeleton and slowed down cell cycle G1-phase progression. Furthermore, repression of FoxQ1 enhanced the migratory capacity of coherent mammary epithelial cells. Gene expression profiling of NM18 cells indicated that FoxQ1 is a relevant downstream mediator of TGF-ß1-induced gene expression changes. This included the differential expression of transcription factors involved in epithelial plasticity, for example, Ets-1, Zeb1, and Zeb2. In summary, this study has elucidated the functional impact of FoxQ1 on epithelial differentiation.
Assuntos
Diferenciação Celular , Células Epiteliais/citologia , Fatores de Transcrição Forkhead/metabolismo , Actinas/metabolismo , Animais , Comunicação Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Tamanho Celular/efeitos dos fármacos , Quinases Ciclina-Dependentes/metabolismo , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Fatores de Transcrição Forkhead/genética , Fase G1/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Técnicas de Silenciamento de Genes , Camundongos , Proteínas dos Microfilamentos/metabolismo , Transporte Proteico/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Fator de Crescimento Transformador beta1/farmacologiaRESUMO
BACKGROUND: An accurate histological diagnosis is of fundamental importance for the therapy and prognosis of many kidney diseases. However, it remains unclear whether a single biopsy is representative of changes in the whole kidney. METHODS: To compare the quantity and quality of renal biopsy material taken from two separate areas from one kidney, we prospectively biopsied the renal cortex at the central third and at one of the kidney poles of 103 consecutive 61 native and 42 transplanted kidneys. With two biopsy cores from each kidney we sampled 14.5 ± 8.5 glomeruli/procedure. RESULTS: The length of the biopsy core, the number of glomeruli/core and the markers of chronic renal damage (degree of interstitial fibrosis, proportion of global or segmental scared glomeruli) were not influenced by biopsy location (pole compared with central third locations). Moreover, there was no significant difference in the number of arteries in biopsies obtained from the two different biopsy areas. The percentage between renal cortex and medulla was not influenced by the biopsy area in all kidneys, but transplanted kidney biopsies contained more medulla than specimens from native kidneys. In patients with native kidneys and lower estimated creatinine clearances, there was a nonsignificant trend towards higher variations in the degree of interstitial fibrosis between the two cores, but a coincidence cannot be excluded. There was no significant difference in global sclerotic glomeruli in regard to the biopsy location. CONCLUSION: We conclude that a renal biopsy composed of two cores from different areas of the kidney provides enough material for histological diagnosis. However, despite the variety of different renal diseases, sampling errors are minimal and obtaining two biopsies from different areas of the kidney does not lead to clinically useful information which would alter the management of patients.
Assuntos
Biópsia/métodos , Rim/patologia , Adulto , Idoso , Feminino , Fibrose , Humanos , Rim/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Artéria Renal , UltrassonografiaRESUMO
HISTORY AND CLINICAL FINDINGS: A 70-year-old woman was admitted to hospital with fatigue, pallor and shortness of breath on mild exertion. In her past medical history only borderline hypertension and allergy to penicillin were to note. INVESTIGATIONS: Actual laboratory findings revealed renal failure with metabolic acidosis and hyperkalaemia. A normochrome normocytic anemia and secondary hyperparathyreoidism were suggestive of a subacute course. The renal biopsy showed histological features of a subacute tubulo-interstitial nephritis. DIAGNOSIS, TREATMENT AND COURSE: The chronic renal failure caused by an interstitial nephritis was treated with corticosteroids and hemodialysis treatment was started. The trigger for AIN could not be found, there was no infectious or systemically disease nor a nephrotoxic medication identified. For nearly six months the patient had taken a homeopathic agent which is a dilution of penicillium chrysogenum. In case of a determined allergy to penicillin, an extract of the fungus producing penicillin could possibly cause an interstitial nephritis. The patient was dialysis-independent with a GFR about 8 - 10 ml/min at the time of discharge. CONCLUSION: With interstitial nephritis all agents should be considered a potential suspect, even homeopathic agents.
Assuntos
Hipersensibilidade a Drogas/complicações , Homeopatia , Falência Renal Crônica/induzido quimicamente , Nefrite Intersticial/induzido quimicamente , Penicilinas , Penicillium chrysogenum , Fitoterapia/efeitos adversos , Extratos Vegetais/toxicidade , Idoso , Hipersensibilidade a Drogas/diagnóstico , Feminino , Taxa de Filtração Glomerular/efeitos dos fármacos , Humanos , Falência Renal Crônica/diagnóstico , Nefrite Intersticial/diagnóstico , Fatores de RiscoRESUMO
Proteinuria as a general symptom of a broad range of different diseases can result from gene mutations of molecules building up the glomerular sieve, from immune-mediated, haemodynamic or metabolic disturbances of the glomerular filter. This filter is not a static barrier but consists of a highly dynamic interacting podocyte foot process to foot process to glomerular basement membrane complex. Its function is to prevent leakage of macromolecules and blood cells into the urine. Molecules like nephrin and podocin are directly involved in the formation of the slit diaphragm located at the end of the foot processes. Other molecules, i.e. CD2AP, play a role in organizing the correct position of the podocytes and its foot processes via controlling intra-cellular actin filaments. Gene mutations coding for these molecules directly cause proteinuric diseases. Autoantibodies or circulating immune complexes can destroy this fragile network of cells and the basement membrane via accumulation of inflammatory cells, cytokines and generation of oxygen radicals. Hemodynamic and metabolic changes as seen in diabetic nephropathy are associated with increased TGF-ss expression and extra-cellular matrix expansion in the mesangium and a decrease of podocyte numbers. Thus, proteinuria is the result of a disturbance of the highly fragile network of cells and the basement membrane on the micro-anatomical and molecular level.
Assuntos
Proteinúria/imunologia , Membrana Basal Glomerular/anatomia & histologia , Membrana Basal Glomerular/fisiologia , Glomerulonefrite Membranosa/complicações , Glomerulosclerose Segmentar e Focal/complicações , Humanos , Nefrite Lúpica/complicações , Podócitos/fisiologia , Proteinúria/etiologiaRESUMO
HISTORY AND FINDINGS: An asymptomatic 70-year-old man was found to have a tumor in the region of the left adrenal gland, having undergone cadaveric kidney transplantation 23 years ago. Two years before this a right nephrectomy had been performed for a renal cell carcinoma. There was no left kidney because of agenesis. 14 years after the kidney transplantation a metastasis of the renal cell carcinoma was identified in the thyroid gland. After its resection no further metastases had been discovered. INVESTIGATIONS: Laboratory tests were unremarkable. But a tumor was detected in the left adrenal gland by computed tomography. DIAGNOSIS, TREATMENT AND COURSE: The left adrenal gland was successfully resected. Its histology confirmed a second metastasis of the renal cell carcinoma 23 years after renal transplantation, but no other metastases were found. CONCLUSIONS: Kidney transplantation can be successfully performed even in patients with pre-existing carcinoma. A late metastasis of a renal carcinoma may occur. Close long-term follow-up of the patients is therefore essential.
Assuntos
Neoplasias das Glândulas Suprarrenais/secundário , Carcinoma de Células Renais/secundário , Neoplasias Renais/patologia , Neoplasias da Glândula Tireoide/secundário , Neoplasias das Glândulas Suprarrenais/diagnóstico por imagem , Neoplasias das Glândulas Suprarrenais/cirurgia , Adrenalectomia , Idoso , Carcinoma de Células Renais/cirurgia , Humanos , Rim/anormalidades , Neoplasias Renais/cirurgia , Transplante de Rim , Masculino , Nefrectomia , Neoplasias da Glândula Tireoide/cirurgia , Fatores de Tempo , Tomografia Computadorizada por Raios XRESUMO
The Wnt signaling pathway, linked to development, has been proposed to be recapitulated during the progressive damage associated with chronic organ failure. Chronic allograft damage following kidney transplantation is characterized by progressive fibrosis and a smoldering inflammatory infiltrate. A modified, Fischer 344 (RT1(lvl)) to Lewis (RT1(l)) rat renal allograft model that reiterates many of the major pathophysiologic processes seen in patients with chronic allograft failure was used to study the progressive disease phenotype and specific gene product expression by immunohistochemistry and transcriptomic profiling. Central components of the Tgfb, canonical Wnt and Wnt-Ca2+ signaling pathways were significantly altered with the development of chronic damage. In the canonical Wnt pathway, Wnt3, Lef1 and Tcf1 showed differential regulation. Target genes Fn1, Cd44, Mmp7 and Nos2 were upregulated and associated with the progression of renal damage. Changes in the Wnt-Ca2+ pathway were evidenced by increased expression of Wnt6, Wnt7a, protein kinase C, Cam Kinase II and Nfat transcription factors and the target gene vimentin. No evidence for alterations in the Wnt planar cell polarity (PCP) pathway was detected. Overall results suggest cross talk between the Wnt and Tgfb signaling pathways during allograft inflammatory damage and present potential targets for therapeutic intervention.
Assuntos
Transplante de Rim , Modelos Animais , Proteínas Wnt/metabolismo , Animais , Diferenciação Celular , Polaridade Celular , Fibrose , Perfilação da Expressão Gênica , Imuno-Histoquímica , Rim/patologia , Masculino , Reação em Cadeia da Polimerase , RNA Mensageiro/genética , Ratos , Ratos Endogâmicos F344 , Ratos Endogâmicos Lew , Transdução de Sinais , Transplante HomólogoRESUMO
Mushrooms of the Cortinarius species are nephrotoxic and can cause severe acute renal failure. The toxic effect is due to orellanine. It is suspected that the cytotoxic damage is caused by the production of oxygen-free radicals. Renal pathology shows tubular necrosis with interstitial nephritis. In addition to accidental intoxications as a consequence of mushroom meals, recent cases are often due to voluntary abuse of natural drugs like magic mushrooms. We report 4 current cases of acute renal failure from intoxication by Cortinarius species by confusing it with psychoactive fungi. Typical for the Cortinarius poisoning is the long latency period from ingestion until the onset of clinical symptoms (3 - 20 days). Diagnosis is based on microscopical identification of the mushroom spores, and detection of the orellanine toxin in leftover mushrooms. In renal biopsy tissue, orellanine is detectable by thin-layer chromaography technique up to 6 months after poisoning. There is no causative therapy, and treatment is symptomatic with adequate hemodialysis. In cases of otherwise unexplained acute renal failure, intoxication with nephrotoxic mushrooms should be considered.
Assuntos
Injúria Renal Aguda/etiologia , Cortinarius/patogenicidade , Rim/ultraestrutura , Intoxicação Alimentar por Cogumelos/complicações , Injúria Renal Aguda/diagnóstico , Injúria Renal Aguda/terapia , Adolescente , Adulto , Cortinarius/isolamento & purificação , Diagnóstico Diferencial , Seguimentos , Humanos , Rim/efeitos dos fármacos , Masculino , Microscopia Eletrônica , Intoxicação Alimentar por Cogumelos/diagnóstico , Diálise Renal , Adulto JovemRESUMO
UNLABELLED: The glycosphingolipids globotrihexosylceramide (Gb3, CD77) and isoglobotrihexosylceramide (iGb3) are isomers differing only in one glycosidic bond and have been implicated in several processes of the innate and adaptive immune system. AIMS: 1) To verify the function of Gb3 in the pathogenesis of hemolytic-uremic syndrome as the cellular receptor responsible for cytotoxicity caused by verotoxin (VT) elaborated by Shigella and certain strains of E.coli. 2) To investigate in vivo the previously implicated function of iGb3 as the endogenous lipid ligand responsible for positive selection of invariant natural killer T-cells (iNKT), which have an essential regulatory function in infection, tumor rejection and tolerance. METHODS: Generation of mice deficient in Gb3 and iGb3 synthesizing enzymes and VT injection into Gb3-deficient mice. Analysis of iNKT cell development and function by flow cytometry and by administration of the exogenous agonist alpha-galactosylceramide in iGb3-deficient mice. RESULTS: For 1) Gb3-deficient mice were insensitive to otherwise lethal doses of VT, and 2) iGb3-deficient mice showed normal numbers of iNKT cells. Furthermore the function of iNKT cells evolving in iGb3-deficient mice was unaffected. CONCLUSIONS: 1) Gb3 is the cellular receptor mediating verotoxin cytotoxicity in haemolytic-uremic syndrome. 2) In contrast to previous indirect implications, iGb3 cannot be regarded as an endogenous ligand responsible for the positive selection of iNKT cells.
Assuntos
Globosídeos/fisiologia , Síndrome Hemolítico-Urêmica/imunologia , Células T Matadoras Naturais/imunologia , Triexosilceramidas/fisiologia , Animais , Citocinas/sangue , Células Dendríticas/imunologia , Escherichia coli/imunologia , Feminino , Globosídeos/genética , Contagem de Linfócitos , Camundongos , Camundongos Knockout , Toxinas Shiga/imunologia , Toxinas Shiga/toxicidade , Shigella/imunologia , Triexosilceramidas/genéticaAssuntos
Pielonefrite Xantogranulomatosa/diagnóstico , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Neoplasias Renais/diagnóstico , Imageamento por Ressonância Magnética , Pielonefrite Xantogranulomatosa/patologia , Insuficiência Renal/diagnóstico , Sepse/diagnóstico , Tomografia Computadorizada por Raios XRESUMO
Macrophages and dendritic cells are heterogenous and highly plastic bone marrow-derived cells that play major roles in renal diseases. We characterized these cells using immunohistochemistry in 55 renal biopsies from control patients or patients with glomerulonephritis as an initial step towards postulating specific roles for these cells in kidney disease. In proliferative glomerulonephritis numerous CD68 positive (pan monocyte, macrophage and dendritic marker) cells were found in both glomeruli and the tubulointerstitial space, however, a myeloid dendritic cell marker (DC-SIGN) was identified only in the tubulointerstitium. A significant number of plasmacytoid dendritic cells (identified as BDCA-2 positive cells) were seen at sites of interstitial inflammation, including follicular aggregates of inflammatory cells. Langerin positive cells (a marker of Langerhans' cells) were detectable but rare. The area of either CD68 or DC-SIGN positive interstitial cells correlated with serum creatinine. Low levels of DC-SIGN, DC-LAMP and MHC class II mRNA were present in the tubulointerstitial space in controls and increased only in that region in proliferative glomerulonephritis. We demonstrate that the CD68 positive cells infiltrating the glomerulus lack dendritic cell markers (reflecting macrophages), whereas in the tubulointerstitial space the majority of CD68 positive cells are also DC-SIGN positive (reflecting myeloid dendritic cells). Their number correlated with serum creatinine, which further emphasizes the significance of interstitial DCs in progressive glomerular diseases.
Assuntos
Células Dendríticas/imunologia , Glomerulonefrite/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos CD , Antígenos de Diferenciação Mielomonocítica , Biomarcadores/análise , Estudos de Casos e Controles , Moléculas de Adesão Celular , Movimento Celular , Progressão da Doença , Glomerulonefrite/patologia , Humanos , Imuno-Histoquímica , Imunofenotipagem , Inflamação , Glomérulos Renais/patologia , Lectinas Tipo C , Pessoa de Meia-Idade , Receptores de Superfície CelularRESUMO
CCN proteins affect cell proliferation, migration, attachment, and differentiation. We identified CCN3 as a suppressed gene following platelet-derived growth factor (PDGF)-BB or -DD stimulation in a cDNA-array analysis of mesangial cells. In vitro growth-arrested mesangial cells overexpressed and secreted CCN3, whereas the addition of the recombinant protein inhibited cell growth. Induction of mesangial cell proliferation by PDGF-BB or the specific PDGF beta-receptor ligand PDGF-DD led to downregulation of CCN3 mRNA, confirming the array study. Specific PDGF alpha-receptor ligands had no effect. CCN3 protein was found in arterial smooth muscle cells, the medullary interstitium, and occasional podocytes in the healthy rat kidney. Glomerular CCN3 was low prior to mesangial proliferation but increased as glomerular cell proliferation subsided during mesangioproliferative glomerulonephritis (GN). Inhibition of PDGF-B in mesangioproliferative disease led to overexpression of glomerular CCN3 mRNA. CCN3 localized mostly to podocytes in human glomeruli, but this expression varied widely in different human glomerulonephritides. Glomerular cell proliferation negatively correlated with CCN3 expression in necrotizing GN. Our study identifies CCN3 as an endogenous inhibitor of mesangial cell growth and a modulator of PDGF-induced mitogenesis.