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1.
Pharmacol Res Perspect ; 10(4): e00993, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35929764

RESUMO

We recently described the identification of a new class of small-molecule activators of the mitochondrial protease ClpP. These compounds synthesized by Madera Therapeutics showed increased potency of cancer growth inhibition over the related compound ONC201. In this study, we describe chemical optimization and characterization of the next generation of highly potent and selective small-molecule ClpP activators (TR compounds) and demonstrate their efficacy against breast cancer models in vitro and in vivo. We selected one compound (TR-107) with excellent potency, specificity, and drug-like properties for further evaluation. TR-107 showed ClpP-dependent growth inhibition in the low nanomolar range that was equipotent to paclitaxel in triple-negative breast cancer (TNBC) cell models. TR-107 also reduced specific mitochondrial proteins, including OXPHOS and TCA cycle components, in a time-, dose-, and ClpP-dependent manner. Seahorse XF analysis and glucose deprivation experiments confirmed the inactivation of OXPHOS and increased dependence on glycolysis following TR-107 exposure. The pharmacokinetic properties of TR-107 were compared with other known ClpP activators including ONC201 and ONC212. TR-107 displayed excellent exposure and serum t1/2 after oral administration. Using human TNBC MDA-MB-231 xenografts, the antitumor response to TR-107 was investigated. Oral administration of TR-107 resulted in a reduction in tumor volume and extension of survival in the treated compared with vehicle control mice. ClpP activation in vivo was validated by immunoblotting for TFAM and other mitochondrial proteins. In summary, we describe the identification of highly potent new ClpP agonists with improved efficacy against TNBC, through targeted inactivation of OXPHOS and disruption of mitochondrial metabolism.


Assuntos
Neoplasias de Mama Triplo Negativas , Animais , Endopeptidase Clp/química , Endopeptidase Clp/metabolismo , Humanos , Camundongos , Mitocôndrias/metabolismo , Proteínas Mitocondriais/metabolismo , Peptídeo Hidrolases/metabolismo , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Neoplasias de Mama Triplo Negativas/metabolismo
3.
ACS Chem Biol ; 14(5): 1020-1029, 2019 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-31021596

RESUMO

ONC201 is a first-in-class imipridone molecule currently in clinical trials for the treatment of multiple cancers. Despite enormous clinical potential, the mechanism of action is controversial. To investigate the mechanism of ONC201 and identify compounds with improved potency, we tested a series of novel ONC201 analogues (TR compounds) for effects on cell viability and stress responses in breast and other cancer models. The TR compounds were found to be ∼50-100 times more potent at inhibiting cell proliferation and inducing the integrated stress response protein ATF4 than ONC201. Using immobilized TR compounds, we identified the human mitochondrial caseinolytic protease P (ClpP) as a specific binding protein by mass spectrometry. Affinity chromatography/drug competition assays showed that the TR compounds bound ClpP with ∼10-fold higher affinity compared to ONC201. Importantly, we found that the peptidase activity of recombinant ClpP was strongly activated by ONC201 and the TR compounds in a dose- and time-dependent manner with the TR compounds displaying a ∼10-100 fold increase in potency over ONC201. Finally, siRNA knockdown of ClpP in SUM159 cells reduced the response to ONC201 and the TR compounds, including induction of CHOP, loss of the mitochondrial proteins (TFAM, TUFM), and the cytostatic effects of these compounds. Thus, we report that ClpP directly binds ONC201 and the related TR compounds and is an important biological target for this class of molecules. Moreover, these studies provide, for the first time, a biochemical basis for the difference in efficacy between ONC201 and the TR compounds.


Assuntos
Antineoplásicos/farmacologia , Endopeptidase Clp/antagonistas & inibidores , Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cromatografia de Afinidade , Endopeptidase Clp/genética , Endopeptidase Clp/metabolismo , Ativação Enzimática , Técnicas de Silenciamento de Genes , Compostos Heterocíclicos de 4 ou mais Anéis/química , Humanos , Imidazóis , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/enzimologia , Piridinas , Pirimidinas
4.
Int J Radiat Biol ; 93(9): 929-936, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28705041

RESUMO

PURPOSE: The EphA2 tyrosine kinase is frequently overexpressed in human tumors that are also treated with radiation. However, few studies have examined the effect of radiation on the EphA2 receptor itself. The purpose of this project was to investigate the impact of radiation on EphA2 to better understand mechanisms of radioresistance. MATERIALS AND METHODS: Cell lines were exposed to X-rays and assayed for changes in EphA2 protein levels and phosphorylation over time by Western blotting. HEK293 cells stably expressing wild-type EphA2 or the S897A mutant were analyzed for cell survival from X-rays. RESULTS: Treatment of different cancer cell lines with 2 Gy of X-rays induced the phosphorylation of EphA2 on S897 but no changes were found in EphA2 total levels or its tyrosine phosphorylation. Radiation-induced S897 phosphorylation was unaffected by an AKT inhibitor but blocked by a MEK or RSK inhibitor. HEK293 cells expressing the EphA2 S897A mutant had a nearly 2-fold lower level of cell survival from X-rays than cells expressing wild-type EphA2. CONCLUSIONS: These findings show that radiation induces S897 EphA2 phosphorylation, an event associated with increased cell survival. Therefore, targeting pathways that mediate EphA2 S897 phosphorylation may be a beneficial strategy to reduce radioresistance.


Assuntos
Sobrevivência Celular/fisiologia , Sobrevivência Celular/efeitos da radiação , Sistema de Sinalização das MAP Quinases/fisiologia , Sistema de Sinalização das MAP Quinases/efeitos da radiação , Fosfosserina/metabolismo , Radiação Ionizante , Receptor EphA2/metabolismo , Relação Dose-Resposta à Radiação , Células HEK293 , Humanos , Fosforilação/efeitos da radiação , Doses de Radiação , Proteínas Quinases S6 Ribossômicas 90-kDa/metabolismo
5.
Cell Rep ; 20(1): 173-187, 2017 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-28683311

RESUMO

As core components of the microRNA-induced silencing complex (miRISC), Argonaute (AGO) proteins interact with TNRC6 proteins, recruiting other effectors of translational repression/mRNA destabilization. Here, we show that LIMD1 coordinates the assembly of an AGO-TNRC6 containing miRISC complex by binding both proteins simultaneously at distinct interfaces. Phosphorylation of AGO2 at Ser 387 by Akt3 induces LIMD1 binding, which in turn enables AGO2 to interact with TNRC6A and downstream effector DDX6. Conservation of this serine in AGO1 and 4 indicates this mechanism may be a fundamental requirement for AGO function and miRISC assembly. Upon CRISPR-Cas9-mediated knockout of LIMD1, AGO2 miRNA-silencing function is lost and miRNA silencing becomes dependent on a complex formed by AGO3 and the LIMD1 family member WTIP. The switch to AGO3 utilization occurs due to the presence of a glutamic acid residue (E390) on the interaction interface, which allows AGO3 to bind to LIMD1, AJUBA, and WTIP irrespective of Akt signaling.


Assuntos
Proteínas Argonautas/metabolismo , Inativação Gênica , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas com Domínio LIM/metabolismo , MicroRNAs/genética , Proteínas Argonautas/genética , Autoantígenos/metabolismo , RNA Helicases DEAD-box/metabolismo , Células HEK293 , Células HeLa , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/química , Peptídeos e Proteínas de Sinalização Intracelular/genética , Proteínas com Domínio LIM/química , Proteínas com Domínio LIM/genética , MicroRNAs/metabolismo , Fosforilação , Ligação Proteica , Processamento de Proteína Pós-Traducional , Proteínas Proto-Oncogênicas/metabolismo , Proteínas de Ligação a RNA/metabolismo
6.
Eur J Ophthalmol ; 27(6): 675-677, 2017 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-28574133

RESUMO

PURPOSE: To evaluate the technical feasibility of isolated Bowman layer (BL) graft preparation by femtosecond laser (FSL) and to compare the ultrastructural morphology to manually dissected grafts. METHODS: Five whole globes were placed in custom-made eye holders and debrided of epithelium. After programming a dissection depth of 20 µm, the FSL was docked into position and 5 isolated BL grafts were created. From 5 additional globes, corneoscleral buttons were procured, mounted in artificial anterior chambers, and stripped of BL via the previously described manual technique. Three specimens from both series were randomly selected and assigned to transmission electron microscopy for ultrastructural evaluation and thickness measurements. RESULTS: All dissections were uneventful and 10 total grafts were produced: 5 by FSL and 5 by manual dissection. Mean graft thickness was 37 (±8.6) µm (n = 3) for the FSL group and 11.7 (±1.6) µm (n = 3) for the manually dissected group. Transmission electron microscopy revealed a thick but relatively smooth posterior cut edge in the FSL group, versus a virtually isolated BL with irregular rests of dispersed stroma in the manually dissected group. CONCLUSIONS: Femtosecond laser may have potential for harvesting intact BL and with a smooth posterior surface, but accompanied by variable amounts of anterior stroma owing to technical limitations.


Assuntos
Lâmina Limitante Anterior/cirurgia , Transplante de Córnea/métodos , Ceratocone/cirurgia , Terapia a Laser/métodos , Doadores de Tecidos , Coleta de Tecidos e Órgãos/métodos , Idoso , Lâmina Limitante Anterior/ultraestrutura , Estudos de Viabilidade , Feminino , Humanos , Masculino , Microscopia Eletrônica de Transmissão , Projetos Piloto , Reprodutibilidade dos Testes
7.
Mol Cancer Res ; 13(6): 1034-43, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25724429

RESUMO

UNLABELLED: Here, evidence suggests that nitric oxide synthases (NOS) of tumor cells, in contrast with normal tissues, synthesize predominantly superoxide and peroxynitrite. Based on high-performance liquid chromatography analysis, the underlying mechanism for this uncoupling is a reduced tetrahydrobiopterin:dihydrobiopterin ratio (BH4:BH2) found in breast, colorectal, epidermoid, and head and neck tumors compared with normal tissues. Increasing BH4:BH2 and reconstitution of coupled NOS activity in breast cancer cells with the BH4 salvage pathway precursor, sepiapterin, causes significant shifts in downstream signaling, including increased cGMP-dependent protein kinase (PKG) activity, decreased ß-catenin expression, and TCF4 promoter activity, and reduced NF-κB promoter activity. Sepiapterin inhibited breast tumor cell growth in vitro and in vivo as measured by a clonogenic assay, Ki67 staining, and 2[18F]fluoro-2-deoxy-D-glucose-deoxyglucose positron emission tomography (FDG-PET). In summary, using diverse tumor types, it is demonstrated that the BH4:BH2 ratio is lower in tumor tissues and, as a consequence, NOS activity generates more peroxynitrite and superoxide anion than nitric oxide, resulting in important tumor growth-promoting and antiapoptotic signaling properties. IMPLICATIONS: The synthetic BH4, Kuvan, is used to elevate BH4:BH2 in some phenylketonuria patients and to treat diseases associated with endothelial dysfunction, suggesting a novel, testable approach for correcting an abnormality of tumor metabolism to control tumor growth.


Assuntos
Progressão da Doença , Neoplasias/metabolismo , Neoplasias/patologia , Óxido Nítrico Sintase/metabolismo , Animais , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Biopterinas/análogos & derivados , Biopterinas/metabolismo , Linhagem Celular Tumoral , Proteínas Quinases Dependentes de GMP Cíclico/metabolismo , Xenoenxertos , Humanos , Camundongos Nus , NF-kappa B/metabolismo , Ácido Peroxinitroso/metabolismo , Pterinas/metabolismo , Superóxidos/metabolismo , Fator de Transcrição 4 , Fatores de Transcrição/metabolismo , beta Catenina/metabolismo
8.
Subcell Biochem ; 85: 133-59, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25201193

RESUMO

In addition to playing roles in the genesis and progression of cancer, mutant p53 also appears to play a significant role in the response to cancer therapy. In response to chemotherapy and radiation, two mainstays of cancer treatment, most cancer cells harboring p53 mutations show a reduced sensitivity compared to cells lacking p53 or those with wild type p53. However, there are also many instances where mutant p53 has shown no effect or enhances cellular sensitivity to chemotherapy and radiation. Similar to the in vitro cellular studies, the majority of clinical studies show a correlation between the presence of mutant p53 in patient tumors and adverse outcomes following treatment with chemotherapy agents or radiation in comparison to tumors with wild-type p53. However, it still remains unclear whether the presence of mutant p53 in tumors can serve as a reliable prognostic factor and aid in treatment planning. Thus, as genomic analysis of patient tumors becomes more cost effective, the role of mutant p53 in tumor responses from cancer therapy ultimately needs to be addressed. This chapter will discuss current mechanisms of how p53 mutations affect cellular responses to chemotherapy and radiation and discuss patient outcomes based on p53 status.


Assuntos
Genes p53 , Mutação , Neoplasias/tratamento farmacológico , Neoplasias/radioterapia , Antineoplásicos/uso terapêutico , Raios gama , Humanos , Neoplasias/genética
9.
Mol Cell ; 50(3): 356-67, 2013 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-23603119

RESUMO

A high-throughput RNA interference (RNAi) screen targeting 542 genes of the human kinome was used to discover regulators of RNAi. Here we report that the proto-oncogene Akt-3/PKBγ (Akt3) phosphorylates Argonaute 2 (Ago2) at S387, which downregulates cleavage and upregulates translational repression of endogenous microRNA (miRNA)-targeted messenger RNAs (mRNAs). We further demonstrate that Akt3 coimmunoprecipitates with Ago2 and phosphorylation of Ago2 at S387 facilitates its interaction with GW182 and localization to cytoplasmic processing bodies (P bodies), where miRNA-targeted mRNAs are thought to be stored and degraded. Therefore, Akt3-mediated phosphorylation of Ago2 is a molecular switch between target mRNA cleavage and translational repression activities of Ago2.


Assuntos
Proteínas Argonautas/genética , MicroRNAs/genética , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Argonautas/metabolismo , Linhagem Celular , Linhagem Celular Tumoral , Regulação para Baixo , Células HEK293 , Células HeLa , Humanos , Fosforilação , Proto-Oncogene Mas , Proteínas Proto-Oncogênicas c-akt/metabolismo , Regulação para Cima
10.
Biochim Biophys Acta ; 1830(3): 2553-61, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23201202

RESUMO

BACKGROUND: Argonaute (Ago) proteins are essential for the biogenesis and function of -20-30 nucleotide long RNAs such as microRNAs (miRNAs). Ago expression increases or decreases under various physiological conditions, although the functional consequences are unknown. In addition, while reduced global miRNA production was shown to enhance cellular transformation and tumorigenesis, how Ago proteins contribute to human diseases has not been reported. METHOD: Ago2, an essential Ago isoform in mammals, was stably expressed in 293 T, the human embryonic kidney cell line, and H1299, the human lung adenocarcinoma cell line. miRNA and mRNA expression was investigated by quantitative PCR and microarray profiling. Cell proliferation and migration was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and scratch assay in the cell cultures, respectively. How Ago2 affected cell growth in vivo was determined by H1299 xenograft tumor growth in mice. Changes in Ago2 expression in human lung cancer samples were investigated by quantitative PCR and immunohistochemistry. RESULTS: Stable Ago2 overexpression elicited specific changes in miRNA and mRNA expression in both 293 T and H1299 cells. It also inhibited cell proliferation and migration in cell cultures as well as xenograft tumor growth in nude mice. Ago2 expression was lower in human lung adenocarcinomas than in the paired, non-cancerous tissues. GENERAL SIGNIFICANCE: We concluded that changes in Ago2 expression might have significant physiological and pathological consequences in vivo.


Assuntos
Adenocarcinoma/genética , Proteínas Argonautas/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/genética , MicroRNAs/genética , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Adenocarcinoma de Pulmão , Animais , Proteínas Argonautas/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Feminino , Perfilação da Expressão Gênica , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Camundongos , Camundongos Nus , MicroRNAs/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transdução de Sinais , Sais de Tetrazólio , Tiazóis , Transfecção , Carga Tumoral , Ensaios Antitumorais Modelo de Xenoenxerto
11.
Methods Mol Biol ; 962: 85-94, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23150439

RESUMO

Protein-protein interaction studies can provide valuable insight into protein function. One of the most practical and high-yielding approaches is immunoprecipitation of a bait protein followed by mass spectrometry to identify co-precipitating proteins. Here we describe an effective and simplified version of this method that can be performed in most laboratories using standard laboratory equipment (apart from the mass spectrometer). We further demonstrate the utility of this method to identify proteins that specifically interact with mutant forms of the tumor suppressor protein, p53.


Assuntos
Proteínas Mutantes/isolamento & purificação , Proteômica/métodos , Proteína Supressora de Tumor p53/isolamento & purificação , Western Blotting , Linhagem Celular Tumoral , Eletroforese em Gel de Poliacrilamida , Humanos , Imunoprecipitação , Espectrometria de Massas , Proteínas Mutantes/genética , Mutação , Transfecção , Proteína Supressora de Tumor p53/genética
12.
Genomics Proteomics Bioinformatics ; 10(5): 239-45, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23200133

RESUMO

MicroRNAs (miRNAs) are approximately 22-nucleotide-long non-coding RNAs that are important regulators of gene expression in eukaryotes. miRNAs are first transcribed as long primary transcripts, which then undergo a series of processing steps to produce the single-stranded mature miRNAs. This article reviews our current knowledge of the mechanism and regulation of mammalian miRNA expression and points out areas of research that may enhance our understanding of how the specificity and efficiency of miRNA production is controlled in vivo.


Assuntos
MicroRNAs/genética , MicroRNAs/metabolismo , Animais , Regulação da Expressão Gênica , Humanos , MicroRNAs/química , Proteínas/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Ribonucleases/metabolismo , Transcrição Gênica
13.
RNA ; 18(11): 2083-92, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22984192

RESUMO

Dicer cleaves double-stranded RNAs (dsRNAs) or precursor microRNAs (pre-miRNAs) to yield ≈ 22-nt RNA duplexes. The pre-miRNA structure requirement for human Dicer activity is incompletely understood. By large-scale in vitro dicing assays and mutagenesis studies, we showed that human Dicer cleaves most, although not all, of the 161 tested human pre-miRNAs efficiently. The stable association of RNAs with Dicer, as examined by gel shift assays, appears important but is not sufficient for cleavage. Human Dicer tolerates remarkable structural variation in its pre-miRNA substrates, although the dsRNA feature in the stem region and the 2-nt 3'-overhang structure in a pre-miRNA contribute to its binding and cleavage by Dicer, and a large terminal loop further enhances pre-miRNA cleavage. Dicer binding protects the terminal loop from digestion by S1 nuclease, suggesting that Dicer interacts directly with the terminal loop region.


Assuntos
RNA Helicases DEAD-box/química , MicroRNAs/química , Clivagem do RNA , Precursores de RNA/química , Ribonuclease III/química , Sequência de Bases , Ensaio de Desvio de Mobilidade Eletroforética , Proteínas Fúngicas/química , Células HEK293 , Humanos , Sequências Repetidas Invertidas , Cinética , MicroRNAs/genética , Dados de Sequência Molecular , Mutação , Conformação de Ácido Nucleico , Ligação Proteica , Endonucleases Específicas para DNA e RNA de Cadeia Simples/química , Especificidade por Substrato
15.
Arch Biochem Biophys ; 518(1): 79-88, 2012 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-22198284

RESUMO

Cancer cells with p53 mutations, in general, grow more aggressively than those with wild-type p53 and show "gain of function" (GOF) phenotypes such as increased growth rate, enhanced resistance to chemotherapeutic drugs, increased cell motility and tumorigenicity; although the mechanism for this function remains unknown. In this communication we report that p53-mediated NF-κB2 up-regulation significantly contributes to the aggressive oncogenic behavior of cancer cells. Lowering the level of mutant p53 in a number of cancer cell lines resulted in a loss of GOF phenotypes directly implicating p53 mutants in the process. RNAi against NF-κB2 in naturally occurring cancer cell lines also lowers GOF activities. In H1299 cells expressing mutant p53, chromatin immunoprecipitation (ChIP) assays indicate that mutant p53 induces histone acetylation at specific sites on the regulatory regions of its target genes. ChIP assays using antibodies against transcription factors putatively capable of interacting with the NF-κB2 promoter show increased interaction of CBP and STAT2 in the presence of mutant p53. Thus, we propose that in H1299 cells, mutant p53 elevates expression of genes capable of enhancing cell proliferation, motility, and tumorigenicity by inducing acetylation of histones via recruitment of CBP and STAT2 on the promoters causing CBP-mediated histone acetylation.


Assuntos
Proteína de Ligação a CREB/metabolismo , Mutação , Subunidade p52 de NF-kappa B/genética , Regiões Promotoras Genéticas/genética , Fatores de Transcrição STAT/metabolismo , Transcrição Gênica/genética , Proteína Supressora de Tumor p53/metabolismo , Acetilação , Animais , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células , Cromatina/genética , Cromatina/metabolismo , Histonas/metabolismo , Humanos , Camundongos , Subunidade p52 de NF-kappa B/deficiência , Subunidade p52 de NF-kappa B/metabolismo , Ligação Proteica/genética , Interferência de RNA , Proteína Supressora de Tumor p53/deficiência , Proteína Supressora de Tumor p53/genética , Regulação para Cima/genética
16.
Arch Biochem Biophys ; 512(1): 52-60, 2011 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-21621504

RESUMO

The p53 gene is one of the most frequently mutated genes in human cancer. Some p53 mutations impart additional functions that promote oncogenesis. To investigate how these p53 mutants function, a proteomic analysis was performed. The protein, translocator of the inner mitochondrial membrane 50 (Tim50), was upregulated in a non-small cell lung carcinoma cell line (H1299) that expressed the p53 mutants R175H and R273H compared to cells lacking p53. Tim50 was also elevated in the breast cancer cell lines MDA-MB-468 and SK-BR-3, that endogenously express the p53 mutants R175H and R273H, respectively, compared to MCF-10A. The p53 mutants R175H and R273H, but not WT p53, upregulated the expression of a Tim50 promoter construct and chromatin immunoprecipitation (ChIP) analysis indicated increased histone acetylation and increased interaction of the transcription factors Ets-1, CREB and CREB-binding protein (CBP) with the Tim50 promoter in the presence of mutant p53. Finally, reduction of Tim50 expression reduced the growth rate and chemoresistance of cells harboring mutant p53 but had no effect upon cells lacking p53. Taken together, these findings identify the Tim50 gene as a transcriptional target of mutant p53 and suggest a novel mechanism by which p53 mutants enhance cell growth and chemoresistance.


Assuntos
Regulação Neoplásica da Expressão Gênica , Proteínas de Membrana Transportadoras/genética , Mutação , Neoplasias/genética , Proteína Supressora de Tumor p53/genética , Neoplasias da Mama/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Humanos , Neoplasias Pulmonares/genética , Mitocôndrias/genética , Proteínas do Complexo de Importação de Proteína Precursora Mitocondrial , Regulação para Cima
17.
Free Radic Biol Med ; 50(6): 749-62, 2011 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-21172423

RESUMO

Models for exploring tyrosine nitration in proteins have been created based on 3D structural features of 20 proteins for which high-resolution X-ray crystallographic or NMR data are available and for which nitration of 35 total tyrosines has been experimentally proven under oxidative stress. Factors suggested in previous work to enhance nitration were examined with quantitative structural descriptors. The role of neighboring acidic and basic residues is complex: for the majority of tyrosines that are nitrated the distance to the heteroatom of the closest charged side chain corresponds to the distance needed for suspected nitrating species to form hydrogen bond bridges between the tyrosine and that charged amino acid. This suggests that such bridges play a very important role in tyrosine nitration. Nitration is generally hindered for tyrosines that are buried and for those tyrosines for which there is insufficient space for the nitro group. For in vitro nitration, closed environments with nearby heteroatoms or unsaturated centers that can stabilize radicals are somewhat favored. Four quantitative structure-based models, depending on the conditions of nitration, have been developed for predicting site-specific tyrosine nitration. The best model, relevant for both in vitro and in vivo cases, predicts 30 of 35 tyrosine nitrations (positive predictive value) and has a sensitivity of 60/71 (11 false positives).


Assuntos
Modelos Moleculares , Nitratos/química , Nitritos/química , Nitrogênio/química , Tirosina/química , Sequência de Aminoácidos , Cristalografia por Raios X , Ligação de Hidrogênio , Imageamento por Ressonância Magnética , Modelos Químicos , Estrutura Molecular , Estresse Oxidativo , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína
18.
Semin Radiat Oncol ; 20(3): 201-7, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20685583

RESUMO

The goal of radiation therapy is to reduce or eliminate tumor burden while sparing normal tissues from long-term injury. Thoracic radiation presents a unique challenge because of the inherent sensitivity of normal lung tissue to radiation. Damage to normal lung tissue presents a major obstacle in the treatment of individuals. To overcome this problem, a number of strategies are being used, including the modulation of dose volume, the use of image-guided radiotherapy, and the use of agents designed to reduce lung injury from radiation. Herein we discuss our current knowledge of the molecular and cellular events that occur after radiation therapy, the clinical manifestations of radiation-induced lung injury, current strategies to minimize lung injury, and recent experimental methods to reduce lung injury and their potential for translation into the clinic.


Assuntos
Neoplasias Pulmonares/radioterapia , Pneumonite por Radiação/etiologia , Planejamento da Radioterapia Assistida por Computador/métodos , Radioterapia Conformacional/efeitos adversos , Relação Dose-Resposta à Radiação , Humanos , Pulmão/efeitos da radiação , Dosagem Radioterapêutica , Radioterapia Assistida por Computador/métodos , Radioterapia Conformacional/métodos
19.
Int J Radiat Oncol Biol Phys ; 78(2): 547-54, 2010 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-20584581

RESUMO

PURPOSE: To identify temporal changes in protein expression in the irradiated rat lung and generate putative mechanisms underlying the radioprotective effect of the manganese superoxide dismutase mimetic MnTE-2-PyP(5+). METHODS AND MATERIALS: Female Fischer 344 rats were irradiated to the right hemithorax with a single dose of 28 Gy and killed from day 1 to 20 weeks after irradiation. Proteomic profiling was performed to identify proteins that underwent significant changes in abundance. Some irradiated rats were administered MnTE-2-PyP(5+) and changes in protein expression and phosphorylation determined at 6 weeks after irradiation. RESULTS: Radiation induced a biphasic stress response in the lung, as shown by the induction of heme oxygenase 1 at 1-3 days and at 6-8 weeks after irradiation. At 6-8 weeks after irradiation, the down-regulation of proteins involved in cytoskeletal architecture (filamin A and talin), antioxidant defense (biliverdin reductase and peroxiredoxin II), and cell signaling (ß-catenin, annexin II, and Rho-guanosine diphosphate dissociation inhibitor) was observed. Treatment with MnTE-2-PyP(5+) partially prevented the apparent degradation of filamin and talin, reduced the level of cleaved caspases 3 and 9, and promoted Akt phosphorylation as well as ß-catenin expression. CONCLUSION: A significant down-regulation of proteins and an increase in protein markers of apoptosis were observed at the onset of lung injury in the irradiated rat lung. Treatment with MnTE-2-PyP(5+), which has been demonstrated to reduce lung injury from radiation, reduced apparent protein degradation and apoptosis indicators, suggesting that preservation of lung structural integrity and prevention of cell loss may underlie the radioprotective effect of this compound.


Assuntos
Pulmão/efeitos da radiação , Metaloporfirinas/farmacologia , Proteínas/metabolismo , Lesões Experimentais por Radiação/metabolismo , Protetores contra Radiação/farmacologia , Animais , Anexina A2/metabolismo , Apoptose , Caspase 9/metabolismo , Proteínas Contráteis/metabolismo , Regulação para Baixo , Feminino , Filaminas , Inibidores de Dissociação do Nucleotídeo Guanina/metabolismo , Heme Oxigenase-1/metabolismo , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Proteínas dos Microfilamentos/metabolismo , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo , Peroxirredoxinas/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Mensageiro/metabolismo , Doses de Radiação , Lesões Experimentais por Radiação/prevenção & controle , Ratos , Ratos Endogâmicos F344 , Talina/metabolismo , Fatores de Tempo , beta Catenina/metabolismo , Inibidores da Dissociação do Nucleotídeo Guanina rho-Específico
20.
Biochemistry ; 49(25): 5331-9, 2010 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-20499882

RESUMO

Previous studies demonstrate that nitric oxide (NO) promotes p53 transcriptional activity by a classical DNA damage responsive mechanism involving activation of ATM/ATR and phosphorylation of p53. These studies intentionally used high doses of NO donors to achieve the maximum DNA damage. However, lower concentrations of NO donors also stimulate rapid and unequivocal nuclear retention of p53 but apparently do not require ATM/ATR-dependent p53 phosphorylation or total p53 protein accumulation. To identify possible mechanisms for p53 activation at low NO levels, the role of Tyr nitration in p53 activation was evaluated. Low concentrations of the NO donor, DETA NONOate (<200 microM), exclusively nitrate Tyr327 within the tetramerization domain promoting p53 oligomerization, nuclear accumulation, and increased DNA-binding activity without p53 Ser15 phosphorylation. Molecular modeling indicates that nitration of one Tyr327 stabilizes the dimer by about 2.67 kcal mol(-1). Significant quantitative and qualitative differences in the patterns of p53-target gene modulation by low (50 microM), non-DNA-damaging and high (500 microM), DNA-damaging NO donor concentrations were shown. These results demonstrate a new posttranslational mechanism for modulating p53 transcriptional activity responsive to low NO concentrations and independent of DNA damage signaling.


Assuntos
Biopolímeros/metabolismo , Nitratos/metabolismo , Doadores de Óxido Nítrico/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Linhagem Celular Tumoral , Ensaio de Desvio de Mobilidade Eletroforética , Humanos , Espectrometria de Massas , Modelos Moleculares , Óxido Nítrico Sintase/metabolismo , Fosforilação , Termodinâmica , Proteína Supressora de Tumor p53/química
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