RESUMO
The results of surgical treatment of 316 patients, suffering focal hepatic diseases, in whom for preoperative preparation a portal vein embolization (PVE) was performed, were analyzed. PVE was applied in a small planned hepatic residual volume. The patients have aged from 21 to 77 yrs, (57 ± 10.6) yrs at average. During (22 ± 7) days after the procedure a hypertrophy of a planned postresectional hepatic volume by 58.6% was observed, while a hypertrophy degree have depended on the embolization volume performed: 57.3%--after embolization of branches of C(V)-C(VIII) hepatic segments, 66%--the segments C(V)-C(VIII) + C(IV). In 281 (89%) patients the extensive hepatic resection was performed, a fatal postresection hepatic insufficiency was not observed. A three-year and five-year disease-free survival have constituted 43.8 and 16.4% accordingly. Thus, a PVE constitutes a miniinvasive intervention, permitting to achieve a planned residual hepatic volume, to expand a diapazon of application of radical extensive hepatic resection in patients, suffering focal hepatic diseases while a small planned residual hepatic volume.
Assuntos
Embolização Terapêutica/métodos , Hepatectomia/métodos , Insuficiência Hepática/cirurgia , Fígado/cirurgia , Veia Porta/cirurgia , Adulto , Idoso , Intervalo Livre de Doença , Embolização Terapêutica/mortalidade , Feminino , Insuficiência Hepática/diagnóstico por imagem , Insuficiência Hepática/mortalidade , Insuficiência Hepática/patologia , Humanos , Fígado/irrigação sanguínea , Fígado/diagnóstico por imagem , Fígado/patologia , Masculino , Pessoa de Meia-Idade , Veia Porta/diagnóstico por imagem , Veia Porta/patologia , Cuidados Pré-Operatórios , RadiografiaRESUMO
The transcription levels of two families of mouse repetitive elements namely intracisternal A particle (IAP) genes, and B2 sequences were analyzed in different tumor cells and normal tissues. These sequences belong to two major classes of mobile elements present in the mouse genome. The Northern blots containing poly(A) + RNAs from tumor cells and normal tissues were hybridized to the cloned IAP gene and B2 sequence. The content of IAP gene transcripts in tumor cells is much higher than in normal cells. A 10- to 100-fold difference was found. The predominant IAP-gene specific RNAs in all investigated tumor cells were 9.5, 6.8 and 5.3 kb long. Additional RNA species were found in some of the tumors. The active synthesis of small cytoplasmic B2 RNA transcribed by RNA polymerase III was also detected in most tumor cells tested. Usually it was higher than in normal cells. Free closed circular DNAs hybridizing to IAP gene probes were cloned from Ehrlich ascites carcinoma cells. We speculate that the data obtained indicate the enhanced transposition of mobile elements in tumor cells which may be an important factor of tumor progression.
Assuntos
Genes de Partícula A Intracisternal , Proto-Oncogenes , RNA/isolamento & purificação , Transcrição Gênica , Animais , Composição de Bases , Linhagem Celular , Clonagem Molecular , Camundongos , Peso Molecular , Neoplasias Experimentais/genética , Hibridização de Ácido Nucleico , RNA/genética , RNA Neoplásico/isolamento & purificação , Sequências Repetitivas de Ácido NucleicoRESUMO
The cytoplasmic poly(A)+RNAs containing ubiquitous B1 and B2 repeats of the mouse genome in normal tissues and tumors have been studied. Only one strand of the repeats is represented in cytoplasmic RNA in all the cases. Some tumor cells were found to be enriched in 1.4 kb B1+mRNA, 1.6 kb B2+mRNA and small (0.2-04 kb) B1+ and B2+ poly(A)+RNAs. On the other hand, mouse liver and kidney contained high amounts of 2 kb B2+mRNA. Its content increased noticeably in the regenerating liver, but in hepatoma it dropped to a zero level. Thus, the switching on (or off) of B1- and B2-containing mRNAs occurred noncoordinately. At the same time, the activation of the synthesis of small B2+RNA and small B1+RNA was simultaneous.