[Alopecia and COVID-19: possible etiopathogenetic variants and therapeutic approach]. / Alopetsiya i COVID-19: vozmozhnye etiopatogeneticheskie varianty i printsipy lecheniya.

Sudden hair thinning, phantom trichalgia in the early and late rehabilitation period after novel coronavirus infection (COVID-19) are the most common complaints of patients, that can be considered by both dermatocosmetologist and medical rehabilitation specialist. A telogen hair loss was found in 19.8% of patients, whereby 27.3% of patients suffering from hair loss during disease and 72.7% - at 3rd-6th month after recovery. Most commonly, hair loss is non-structural and associated with an abnormal ovulatory cycle shift and diffuse asynchronous loss of hair follicles in telogen phase, as well as with an increase of total predisposed to loss hair follicles number. Nevertheless, the analysis of clinical observations of patients with post-COVID hair loss has shown that this disorder is registered not only in telogen phase. There is a rapid disease progression up to the final stages in the presence of verified androgenetic alopecia diagnosis. The cases of alopecia areata and cicatricial alopecia, associated with previous COVID-19, also were registered. Androgenetic alopecia is the first (30.7%) and diffuse alopecia is the second (19.8%) by degree of incidence. The relapses or much less frequently the onsets of alopecia areata and the unexplained pronounced pain at the hair roots in parietal region (7.8%) are in the third place. The article presents in detail the possible reasons and mechanisms of hair loss associated with COVID-19, determines necessary examinations with consideration to the scientific analysis of domestic and foreign literature sources.

[Use of polymeric suspensions as a viral sorbent to detect cattle serum antibodies].

The paper shows it possible to use stained polymeric microspheres, 1.7 microm in diameter, that contain viruses onto the surface, in the latex agglutination test to detect antibodies to the bovine serum viruses of infective rhinotracheitis, parainfluenza-3, viral diarrhea, respiratory syncytial infection, and adenoviral infection.

[Phagocytosis of polymer microspheres immobilized with bioligands by peripheral blood leukocytes in patients with acute pancreatitis]. / Fagotsitirovanie polimernykh mikrosfer, immobilizovannykh bioligandami, leikotsitami perifericheskoi krovi u bol'nykh ostrym pankreatitom.

Phagocytosis of polymer microspheres both modified and unmodified by bioligands (gelatin, fibronectin and A protein) by leukocytic blood cells of patients with acute pancreatitis. The presence of bioligands in the surface of polymer microspheres and their activity were monitored by the reaction of latex agglutination with an appropriate ligand being involved. The study resulted in designing a method of phagocytosis implementation that can be used to evaluate the content of certain receptors in cellular membranes and, subsequently, to define the changed receptor cell apparatus caused by a disease. The nature of polymer microspheric bioligands immobilized to the surface was shown to influence significantly the activity of leukocytic blood cells.

[The production of immunochemical reagents for the determination of C-reactive protein by latex agglutination]. / Poluchenie immunokhimicheskikh reagentov dlia opredeleniia C-reaktivnogo belka metodom lateksnoi aggliutinatsii.

A new simple and convenient method for the detection of C-reactive protein (CRP) in patients' blood sera is based on latex agglutination making use of new compounds obtained by conjugation of IgG fraction containing goat antibodies to CRP with various latex particles. The sensitivity of the methods is 3 mg/ml, this permitting its wide clinical application.

[The determination of opiates by latex agglutination]. / Opredelenie opiatov metodom lateksnoi aggliutinatsii.

A method based on latex agglutination test has been developed for detection of opiates in human urine. Specific reagents were synthesized and conditions of interaction of conjugated latex antigens with specific antibodies to morphine in the presence of an inhibitor were examined. Sensitivity of the method was 100 ng/ml. The developed latex agglutination test for detection of opiates may be used in combination with enzyme immunoassay in practical forensic medicine and narcology.

[Study of the latex agglutination reaction using a light-scattering method]. / Issledovanie reaktsii lateksnoi aggliutinatsii metodom svetorasseianiia.

Light scattering techniques as a method for monitoring latex agglutination test were under study. The model system, employed in the studies, consisted of a suspension containing polystyrene latex microspheres with tetanus antitoxin immobilized on them (carrier particles) and antitetanus serum (3000 IU). The studies helped define approaches to the choice of the optimal conditions for monitoring the latex agglutination test. Light scattering method has proved to be highly sensitive, its sensitivity essentially surpassing that of slide tests and permitting the detection of early agglutination stages.

[Identification of digitonin binding sites on the plasma membrane of the rabbit aorta endotheliocytes]. / Identifikatsiia digitoninsviazyvaiushchikh uchastkov plasmaticheskoi membrany éndoteliotsitov aorty krolika.

Specific reagents comprising digitonin bound to latex spheres were used as visual markers for the detection of cholesterol sites on the endothelial cell surface by scanning electron microscopy. The distribution of latex markers in the plasmolemma of the endothelial cells was investigated. These markers have strong bonds with the ligands. This interaction guarantees high specificity of binding between labeled markers and cellular membrane cholesterol.

[Cholesterol in the plasmalemma of endotheliocytes in the normal aorta and in hypercholesterolemia (scanning electron microscopy research)]. / Kholesterin v plazmolemme éndoteliotsitov aorty v norme i pri giperkholesterinemii (skaniruiushchee élektronno-mikroskopicheskoe issledovanie).

Using a specific latex marker (SLM) and scanning electron microscopy, distribution of cholesterin in plasmolemma of endotheliocytes of the thoracic part of the rabbit aorta has been studied under normal conditions and at experimental atherosclerosis, as well as on the surface of liposomes and flat bilayer films. SLM reacts with a high specificity directly with cholesterin of the membrane. When studying microtopography of cholesterin, it is possible to distinguish microdomains with various density of distribution of SLM particles. Amount of SLM per 1 mcm2 of the artificial membrane surfaces grows at increase of the cholesterin-phospholipid (Ch/Phl) index of the membrane; Ch/Phl can bind with the membranes beginning only with the threshold value of the Ch/Phl index equal to 0.5. At hypercholesterolemia amount of SLM on the luminal surface of plasmolemma of the aortal endotheliocytes increases by 78%. Hence, at hypercholesterolemia in the luminal part of plasmolemma of the aortal endotheliocytes the Ch/Phl index increases; this should be taken into consideration, when observing mechanisms of reorganization of the endothelial cells layer at atherosclerosis.