RISE: an open-source architecture for interdisciplinary and reproducible human-robot interaction research.

In this article, we present RISE-a Robotics Integration and Scenario-Management Extensible-Architecture-for designing human-robot dialogs and conducting Human-Robot Interaction (HRI) studies. In current HRI research, interdisciplinarity in the creation and implementation of interaction studies is becoming increasingly important. In addition, there is a lack of reproducibility of the research results. With the presented open-source architecture, we aim to address these two topics. Therefore, we discuss the advantages and disadvantages of various existing tools from different sub-fields within robotics. Requirements for an architecture can be derived from this overview of the literature, which 1) supports interdisciplinary research, 2) allows reproducibility of the research, and 3) is accessible to other researchers in the field of HRI. With our architecture, we tackle these requirements by providing a Graphical User Interface which explains the robot behavior and allows introspection into the current state of the dialog. Additionally, it offers controlling possibilities to easily conduct Wizard of Oz studies. To achieve transparency, the dialog is modeled explicitly, and the robot behavior can be configured. Furthermore, the modular architecture offers an interface for external features and sensors and is expandable to new robots and modalities.

Scaffolding the human partner by contrastive guidance in an explanatory human-robot dialogue.

Explanation has been identified as an important capability for AI-based systems, but research on systematic strategies for achieving understanding in interaction with such systems is still sparse. Negation is a linguistic strategy that is often used in explanations. It creates a contrast space between the affirmed and the negated item that enriches explaining processes with additional contextual information. While negation in human speech has been shown to lead to higher processing costs and worse task performance in terms of recall or action execution when used in isolation, it can decrease processing costs when used in context. So far, it has not been considered as a guiding strategy for explanations in human-robot interaction. We conducted an empirical study to investigate the use of negation as a guiding strategy in explanatory human-robot dialogue, in which a virtual robot explains tasks and possible actions to a human explainee to solve them in terms of gestures on a touchscreen. Our results show that negation vs. affirmation 1) increases processing costs measured as reaction time and 2) increases several aspects of task performance. While there was no significant effect of negation on the number of initially correctly executed gestures, we found a significantly lower number of attempts-measured as breaks in the finger movement data before the correct gesture was carried out-when being instructed through a negation. We further found that the gestures significantly resembled the presented prototype gesture more following an instruction with a negation as opposed to an affirmation. Also, the participants rated the benefit of contrastive vs. affirmative explanations significantly higher. Repeating the instructions decreased the effects of negation, yielding similar processing costs and task performance measures for negation and affirmation after several iterations. We discuss our results with respect to possible effects of negation on linguistic processing of explanations and limitations of our study.

Technologies for Single-Cell Isolation.

The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field.

Single-cell printer: automated, on demand, and label free.

Within the past years, single-cell analysis has developed into a key topic in cell biology to study cellular functions that are not accessible by investigation of larger cell populations. Engineering approaches aiming to access single cells to extract information about their physiology, phenotype, and genotype at the single-cell level are going manifold ways, meanwhile allowing separation, sorting, culturing, and analysis of individual cells. Based on our earlier research toward inkjet-like printing of single cells, this article presents further characterization results obtained with a fully automated prototype instrument for printing of single living cells in a noncontact inkjet-like manner. The presented technology is based on a transparent microfluidic drop-on-demand dispenser chip coupled with a camera-assisted automatic detection system. Cells inside the chip are detected and classified with this detection system before they are expelled from the nozzle confined in microdroplets, thus enabling a "one cell per droplet" printing mode. To demonstrate the prototype instrument's suitability for biological and biomedical applications, basic experiments such as printing of single-bead and cell arrays as well as deposition and culture of single cells in microwell plates are presented. Printing efficiencies greater than 80% and viability rates about 90% were achieved.

Exploitation and commercialization within the EU FP7 project PASCA.

Within recent years, the analysis of single biological cells has emerged as key topic in cell-biology research. Studying cells and cellular functions within large cell populations is still common practice in the life-sciences, but is hampered by several limitations. True access to a cell's physiology, phenotype and genotype can often only be achieved on a single cell level. The European research project "Platform for Advanced Single Cell Analysis" (PASCA) focuses on developing an approach towards inkjet-like printing of single cells. A fully automated prototype instrument for printing of single living cells has been developed, and its potential use is currently being investigated in various biological applications. The various elements of project design, management, and implementation developed within this project to support the commercial exploitation and dissemination of the project results will be discussed. Based on the dedicated project structure of involving external non-funded participants from the beginning of the project and extending it to the preparation of a prototype related market study, various activities aimed at commercializing the project results are presented. We present the lessons learned from those activities alongside the technical results achieved with the PASCA prototype instruments and the perspectives for future commercialization.

Pacientes psiquiátricos crônicos vivendo na comunidade: um estudo piloto / Patients with chronic mental illness living in the community: a pilot study

Os autores realizaram um estudo piloto com o objetivo de identificar e abordar os casos atendidos em dois ambulatórios especializados do Serviço de Psiquiatria do Hospital de Clínicas de Porto Alegre (HCPA). Dez sujeitos foram entrevistados: 90 por cento referiram bom vínculo com a instituiçäo e a principal crítica foi o tempo de espera para a a consulta. O estudo é parte integrante de um projeto de extensäo de cuidados de saúde mental desenvolvido em conjunto pelo Serviço de Psiquiatria do HCPA e o Departamento de Psiquiatria e Medicina Legal da UFGRS