RESUMO
This paper deals with the chiral separation of optical isomers of beta-methyl-amino acids by CE and HPLC using the principle of ligand-exchange. Capillary zone electrophoresis was carried out using Cu(II) complexes of L-4-hydroxyproline (L-4-Hypro), N-(2-hydroxypropyl)-L-4-hydroxyproline (HP-L-4-Hypro) and N-(2-hydroxyoctyl)-L-4-hydroxyproline (HO-L-4-Hypro) as chiral selectors, added to the electrolyte. The HPLC separations were performed on a chiral stationary ligand-exchange chromatography phase containing L-4-Hypro chemically bonded to silica gel. With both techniques nearly all compounds investigated are baseline resolved using different background electrolytes and mobile phases, respectively.
Assuntos
Aminoácidos/análise , Aminoácidos/química , Cromatografia Líquida de Alta Pressão/métodos , Eletroforese Capilar/métodos , Ligantes , EstereoisomerismoRESUMO
This paper deals with the enantioseparation of hydroxy acids by ligand-exchange capillary electrochromatography. A chiral continuous bed was easily prepared by in situ polymerization of monomers, including an L-4-hydroxyproline derivative. This phase showed chiral recognition for several hydroxy acids, in addition to amino acids.
Assuntos
Eletroforese Capilar/métodos , Hidroxiácidos/isolamento & purificação , Aminoácidos/química , Aminoácidos/isolamento & purificação , Hidroxiácidos/química , Ligantes , Ácidos Mandélicos/química , Ácidos Mandélicos/isolamento & purificação , Polímeros , EstereoisomerismoRESUMO
This review summarizes the application of capillary electrophoresis and capillary electrochromatography for the chiral separation of various substance classes using the principle of ligand exchange. The application of this principle to various substance classes is reported.
Assuntos
Cromatografia/métodos , Eletroforese Capilar/métodos , Ligantes , Modelos Químicos , Estereoisomerismo , Fatores de TempoRESUMO
A chiral ligand-exchange phase for capillary electrochromatography based on continuous bed technology was developed. The chiral stationary phase is prepared by a one-step in situ copolymerization procedure using methacrylamide, piperazine diacrylamide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chiral separation of underivatized amino acids is demonstrated.