I think therefore I am: Rest-related prefrontal cortex neural activity is involved in generating the sense of self.

The sense of self has always been a major focus in the psychophysical debate. It has been argued that this complex ongoing internal sense cannot be explained by any physical measure and therefore substantiates a mind-body differentiation. Recently, however, neuro-imaging studies have associated self-referential spontaneous thought, a core-element of the ongoing sense of self, with synchronous neural activations during rest in the medial prefrontal cortex (PFC), as well as the medial and lateral parietal cortices. By applying deep transcranial magnetic stimulation (TMS) over human PFC before rest, we disrupted activity in this neural circuitry thereby inducing reports of lowered self-awareness and strong feelings of dissociation. This effect was not found with standard or sham TMS, or when stimulation was followed by a task instead of rest. These findings demonstrate for the first time a critical, causal role of intact rest-related PFC activity patterns in enabling integrated, enduring, self-referential mental processing.

Calcium efflux mechanism in sperm mitochondria.

This paper reports an investigation on calcium efflux mechanism in ram sperm mitochondria. Energized sperm mitochondria take up Ca2+ via the ruthenium-red sensitive uniporter, and possess a ruthenium-red insensitive efflux mechanism. Extramitochondrial Na+ did not affect the rate of Ca2+ efflux indicating that Na+/Ca2+ exchange mechanism is not involved. Depolarization of inner mitochondrial membrane induced by the uncoupler carbonylcyanide-p-trifluoromethoxyphenyl hydrazone (FCCP) or by the organomercurial SH-reagent mersalyl, causes high stimulation in Ca2+ efflux. This stimulated Ca2+ efflux determined in the presence of ruthenium-red and phosphate, is not inhibited by cyclosporin A (CSA), indicating that mitochondrial permeability transition pore (MTP) is not involved in this Ca2+ efflux mechanism. The stimulated Ca2+ efflux is inhibited by ADP or atractyloside suggesting that the Ca2+ transport mechanism might be intrinsic to the ADP/ATP carrier (AAC). Thus, the data indicate that sperm mitochondria contain a Ca2+ efflux mechanism operated via AAC and regulated by mitochondrial membrane potential and by ADP concentration.