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1.
Transplant Proc ; 45(5): 1957-60, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23769082

RESUMO

BACKGROUND: Hepatocellular carcinoma (HCC) is among the most frequent malignant diseases worldwide. In the vast majority of cases, it is associated with liver cirrhosis. Liver transplantation (OLT) is potentially the gold standard treatment for patients suffering HCC in cirrhosis, because of synchronous eradication of HCC and of the underlying hepatic disease. The aim of this study was to evaluate long-term outcomes of OLT in HCC patients. MATERIAL AND METHODS: Between January 2000 and December 2011, 43 patients who were diagnosed with HCC in liver cirrhosis and underwent OLT in our department, were identified from a prospective database. All patients received their grafts from deceased donors. We analyzed demographic data, laboratory values, number and size of lesions, primary liver disease, diagnostic methods, bridging therapy modalities, and postoperative outcomes, including complications, recurrences, and their treatment. RESULTS: Patient follow-up as of January 2012 or to death ranged from 0 to 138 months (median, 59; mean, 63). None of the patients were lost to follow-up. The gender bias was 85%:15% (male:female) and the median age, 57.8 years (range, 44-69). The most common underlying diseases for cirrhosis and HCC were alcoholic (n = 12) and hepatitis C (n = 16). Thirty-one subjects underwent bridging therapy through transarterial chemoembolization (TACE), and/or radiofrequency ablation. All patients underwent OLT within the Milan criteria according to the preoperative evaluation and histopathologic examination of the explanted liver. Twenty-one of them suffered postoperative complications (48.8%). HCC recurrence, which occurred in 5 (10.4%), was treated by surgery (n = 3), systemic chemotherapy with sorafenib (n = 1), or TACE (n = 1). CONCLUSIONS: OLT for HCC in cirrhosis, displays a relatively high complication rate. It shows good survivals with and low recurrence.


Assuntos
Carcinoma Hepatocelular/cirurgia , Neoplasias Hepáticas/cirurgia , Transplante de Fígado , Adulto , Idoso , Carcinoma Hepatocelular/complicações , Feminino , Alemanha , Humanos , Neoplasias Hepáticas/complicações , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento
2.
Eur J Neurosci ; 20(6): 1499-513, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15355317

RESUMO

FMRFamides belong to the most extensively studied neuropeptides in invertebrates and exhibit diverse physiological effects on different target organs, such as muscles, intestine and the nervous system. This study on the American cockroach confirms for the first time that extended FMRFamides occur in non-dipteran insects. By means of tandem mass spectrometry, these neuropeptides were structurally elucidated, and sequence information was used for subsequent cloning of the cockroach FMRFamide gene. This precursor gene encodes for 24 putative peptides and shows sufficient similarity with the Drosophila FMRFamide gene. Of the 24 peptides, 23 were detected by mass spectrometric methods; it is the highest number of neuropeptide forms shown to be expressed from a single precursor in any insect. The expression was traced back to single neurons in the thoracic ganglia. The unique accumulation of these FMRFamide-related peptides in thoracic perisympathetic organs provides the definite evidence for a tagma-specific distribution of peptidergic neurohormones in neurohaemal release sites of the insect CNS. Excitatory effects of the cockroach FMRFamides were observed on antenna-heart preparations. In addition, the newly described FMRFamides reduce the spike frequency of dorsal-unpaired median neurons and reduce the intracellular calcium concentration, which may affect the peripheral release of the biogenic amine octopamine.


Assuntos
Baratas/metabolismo , FMRFamida/metabolismo , Proteínas de Insetos/metabolismo , Neurônios/fisiologia , Neuropeptídeos/metabolismo , Potenciais de Ação/fisiologia , Sequência de Aminoácidos , Animais , Southern Blotting/métodos , Cálcio/metabolismo , Clonagem Molecular/métodos , Baratas/química , Relação Dose-Resposta a Droga , FMRFamida/química , FMRFamida/farmacologia , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/efeitos dos fármacos , Gânglios dos Invertebrados/fisiologia , Imuno-Histoquímica/métodos , Técnicas In Vitro , Espectrometria de Massas/métodos , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Neurônios/efeitos dos fármacos , Neuropeptídeos/química , Neuropeptídeos/farmacologia , Técnicas de Patch-Clamp/métodos , RNA Mensageiro/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
3.
Vet Radiol Ultrasound ; 41(2): 168-71, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10779078

RESUMO

The aim of this study was to determine the blood flow in the carotid artery during halothane anaesthesia by means of duplex-sonography. Seventeen warm blood horses were evaluated in dorsal and lateral recumbency and the results were compared with the values of the same horses resting and under sedation. The cross sectional area of the vessels, the time-averaged maximal blood velocity, time-averaged maximal blood flow and the resistance index were determined and the flow profile was evaluated. During halothane anesthesia the total blood flow shows a significant increase which is not dependent on the positioning of the horse. Mean blood velocity is decreased by sedation and significantly increased during anesthesia. Reduced peripheral resistance is expressed by a decline of the resistance index. After sedation and during recumbency there is an increase in diameter of the carotid artery.


Assuntos
Anestésicos Inalatórios/farmacologia , Artérias Carótidas/diagnóstico por imagem , Artérias Carótidas/fisiologia , Sedação Consciente/veterinária , Halotano/farmacologia , Cavalos/fisiologia , Animais , Velocidade do Fluxo Sanguíneo/efeitos dos fármacos , Artérias Carótidas/efeitos dos fármacos , Feminino , Masculino , Fluxo Sanguíneo Regional/efeitos dos fármacos , Descanso/fisiologia , Ultrassonografia Doppler/veterinária , Resistência Vascular/efeitos dos fármacos
4.
Cell Tissue Res ; 295(1): 159-70, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9931362

RESUMO

A highly specific polyclonal antiserum has been raised against periviscerokinin, the first neuropeptide isolated from the perisympathetic organs of insects (Predel et al. 1995). In this study, two different neuronal systems with periviscerokinin-like immunoreactivity were distinguished in the central nervous system of the American cockroach: (1) An intrinsic neuronal network, restricted to the head-thoracic region, was formed by intersegmental projecting neurons of the brain, suboesophageal ganglion and metathoracic ganglion. In addition, groups of local interneurons occurred in the proto- and tritocerebrum. (2) A typical neurohormonal system was stained exclusively in the abdomen; it was represented by abdominal perisympathetic organs which were supplied by three cell clusters located in each unfused abdominal ganglion. As revealed by nickel backfills, most neurons with axons entering the perisympathetic organs contained a periviscerokinin-like peptide. Immunoreactive fibres left the perisympathetic organs peripherally, innervated the hyperneural muscle and ran via the link nerves/segmental nerves to the heart and segmental vessels. All visceral muscles innervated by periviscerokinin-immunoreactive fibres were shown to be sensitive to periviscerokinin, whereas the hindgut gave no specific response to this peptide.


Assuntos
Sistema Nervoso/metabolismo , Neuropeptídeos/metabolismo , Periplaneta/metabolismo , Animais , Imuno-Histoquímica , Periplaneta/citologia
5.
Arq Neuropsiquiatr ; 57(4): 907-11, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10683677

RESUMO

The mutation in the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene has been determined in two brothers affected with Lesch-Nyhan syndrome. Female members of the family who are at risk for being heterozygous carriers of the HPRT mutation were also studied to determine whether they carry the mutation. DNA sequencing revealed that the boys' mother is heterozygous for the mutation in her somatic cells, but that three maternal aunts are not heterozygous. Such carrier information is important for the future pregnancy plans of at-risk females. The mutation, an A-->T transversion at cDNA base 590 (590A-->T), results in an amino acid change of glutamic acid to valine at codon 197, and has not been reported previously in a Lesch-Nyhan syndrome male. This mutation is designated HPRTBrasil.


Assuntos
Hipoxantina Fosforribosiltransferase/genética , Síndrome de Lesch-Nyhan/genética , Mutação Puntual , Adolescente , Adulto , Brasil , Criança , DNA Complementar/análise , Feminino , Heterozigoto , Humanos , Síndrome de Lesch-Nyhan/diagnóstico , Masculino , Linhagem , Fatores Sexuais
6.
J Morphol ; 223(2): 225-42, 1995 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-7877184

RESUMO

The neuronal connections of the tritocerebral commissures of Periplaneta americana were studied in the brain-suboesophageal ganglion complex and the stomatogastric nervous system by means of heavy metal iontophoresis through cut nerve ends followed by silver intensification. The tritocerebral commissure 1 (Tc1) contains mainly the processes of the subpharyngeal nerve (Spn) whose neurons are located in both tritocerebral lobes and in the frontal ganglion. Some neurons of the frontal ganglion project through the Tc1 to the contralateral tritocerebrum. A few fibers in this commissure were observed projecting to the protocerebrum and the suboesophageal ganglion. There are tritocerebral neurons which pass through the Tc1 or the tritocerebral commissure 2 (Tc2) and extend on into the stomatogastric nervous system. One axon of a descending giant neuron appears in the Tc2. This neuron lies in the tritocerebrum and connects the brain to the contralateral side of the ventral nerve cord. In addition, sensory fibers of the labral nerve (Ln) traverse both commissures to the opposite tritocerebrum. The anatomical and physiological relevance of the identified neuronal pathways is discussed.


Assuntos
Encéfalo/citologia , Neurônios/ultraestrutura , Periplaneta/citologia , Animais , Axônios/ultraestrutura , Encéfalo/fisiologia , Sistema Digestório/inervação , Gânglios dos Invertebrados/citologia , Fígado/fisiologia , Masculino , Sistema Nervoso/citologia , Fenômenos Fisiológicos do Sistema Nervoso , Vias Neurais/citologia , Vias Neurais/fisiologia , Periplaneta/fisiologia , Transmissão Sináptica/fisiologia
7.
Cell Tissue Res ; 208(2): 283-97, 1980.
Artigo em Inglês | MEDLINE | ID: mdl-6157472

RESUMO

The neuronal pathways connecting the stomatogastric nervous system with the retrocerebral complex of the cockroach, Periplaneta americana, were investigated by means of axonal cobalt chloride iontophoresis. Somata in the hypocerebral ganglion and in the nervus recurrens sending their axons to different parts of the stomatogastric nervous system were traced. Some axons in the oesophageal nerve arise from large perikarya in the anterior part of the pars intercerebralis and pass via the NCCI to the corpora cardiaca and the oesophageal nerve. The form a profuse dendritic tree in the protocerebrum. Fibers of the NCC I and NCC II as well as the NCA I and NCA II enter the stomatogastric nervous system via the hypocerebral ganglion.


Assuntos
Baratas/anatomia & histologia , Sistema Nervoso/ultraestrutura , Periplaneta/anatomia & histologia , Animais , Transporte Axonal , Cobalto , Histocitoquímica , Masculino , Microscopia Eletrônica , Sistema Nervoso/anatomia & histologia , Sistema Nervoso/metabolismo , Periplaneta/metabolismo
8.
Cell Tissue Res ; 193(2): 353-71, 1978 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-719723

RESUMO

The frontal ganglion of the cockroach Periplaneta americana was studied histologically and its neuronal pathways were mapped by use of axonal cobalt iontophoresis. Neurons and fiber tracts of the frontal ganglion are directly linked with different regions of the central nervous system (tritocerebrum, protocerebrum, subesophageal ganglion) and with the more caudal parts of the stomatogastric nervous system (hypocerebral ganglion, nervus oesophagei).


Assuntos
Baratas/anatomia & histologia , Gânglios Autônomos/anatomia & histologia , Vias Neurais/anatomia & histologia , Periplaneta/anatomia & histologia , Animais , Iontoforese , Modelos Neurológicos
9.
Z Klin Chem Klin Biochem ; 13(8): 351-3, 1975 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-1216960

RESUMO

In a group of 15 cases the cortisol concentrations were determined in fetal and maternal plasma during labour and after delivery. In maternal plasma the levels were about twice as high as in fetal plasma and rose up to more than 1000 mug/l during labour (x = 639 +/- 222). After delivery the concentrations decreased. In fetal plasma, cortisol increased during labour from x = 173 +/- to x = 276 +/- 75 at the time of delivery and decreased to x = 106 +/- 36 within the first 23 hours. In a second group of 20 cases maternal and umbilical cord blood and blood of the newborn during the first 28 hours after delivery were analyzed. The values were in the same ranges as in the first group. Extremely high maternal levels were not correlated to higher fetal values. The cortisol levels of postmature children did not significantly differ from those of normal babies.


Assuntos
Hidrocortisona/sangue , Feminino , Sangue Fetal/análise , Humanos , Recém-Nascido , Trabalho de Parto , Troca Materno-Fetal , Gravidez
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