Common Feeding Practices Used by Chinese Caregivers of 6-10-Month-Old Infants: A Behavioral Observation Study in Shaanxi Province.

OBJECTIVE: To explore the feeding practices and feeding environment of Chinese families with 6-10-month-old infants. DESIGN: One day of caregiver-recorded feeding occasions. SETTING: Homes in Shaanxi, China. PARTICIPANTS: Families recruited using convenience sampling. VARIABLES MEASURED: Videos were coded for feeding practice frequency and acceptance rate, feeding environment, and responsiveness to infant fullness cues and eating pace. ANALYSIS: Wilcoxon rank sum tests examined the differences in feeding practice use based on caregiver type, infant sex, and infant weight status. RESULTS: Twenty-eight families provided videos for coding. The most commonly observed feeding practices were opening the mouth, giving instructions, and interfering with the child's actions. Prompts to eat were accepted 86.9% of the time. Overweight infants' caregivers used significantly more coercive prompts to eat than did caregivers of healthy-weight infants (P < 0.05). Mothers used more autonomy-supportive prompts to eat than did fathers (P < 0.05). Early, active, and late infant fullness cues were captured in 25.6%, 34.8%, and 8.5% of videos, respectively. 53.6% of caregivers fed at the right pace, whereas 14.5% and 31.9% fed too slow or too fast, respectively. Approximately 5.5% of videos had a screen on, and 33.5% of videos included at least 1 other distraction during the meal. CONCLUSIONS AND IMPLICATIONS: Differences in feeding practices among caregivers suggest that targeted advice may further improve feeding practices. Improving the caregiver's ability to identify satiety cues and respond to infant eating speed may also reduce the risk of overfeeding.

Potential benefits of cropping pattern change in the climate-sensitive regions of rice production in China.

Rice production is a primary contributor to global greenhouse gas emissions, with unclear pathways towards carbon neutrality. Here, through a comprehensive assessment of direct greenhouse gas (GHG) emission using DNDC model and indirect GHG emission using emission factor methods, we estimated the annual crop yield, GHG emission amount and intensity, and economic benefits of different cropping patterns in the climate-sensitive regions of rice production in China. Through the expansion of single-rice and cropping pattern change from the wheat-rice to wheat-rice-rice in the climate-sensitive regions of single and triple-cropping cultivations, the total grain yield increased by 4.4 % and 4.5 % compared with the current national grain production, the GHG emission would increase by 2.4 % and 5.4 % of the current national GHG emissions from rice and wheat production, the net economic benefits could increase 0.9 % and decrease 2.0 % of the national output value of rice and wheat production. The study takes the entire-life cycle of crop growth as the principal line, and could provide a valuable reference for the regulation of the cropping pattern and the formulation of carbon reduction policies in the climate-sensitive region.

Optimizing cultivars and agricultural management practices can enhance soybean yield in Northeast China.

Soybean is an important oil crop in China, and the national focus of soybean production is Northeast China. Crop yield is affected by climate, cultivars and agricultural management practices. Optimizing the composite impacts of these factors on soybean yield and yield gaps is crucial for the local agricultural community. In this study, we used the DSSAT-CROPGRO-Soybean model (validated based on longer-than-20-years agro-meteorological experiments data) to simulate the potential yield (Yp), attainable yield (Ya), and potential farmer's yield (Ypf) of soybean for 56 counties from 1981 to 2017 in Northeast China. Combined with actual farmer's yield (Yf), we computed different types of yield gaps. Furthermore, we optimized cultivars, agricultural management practices, and those interactions on soybean yield and yield gaps. On county-level, the Yp, Ya, Ypf and Yf averaged 5528.9, 4762.9, 3786.8 and 1918.8 kg ha-1, respectively. The total yield gap between Yf and Yp was 63.8 % of Yp. The yield gap between Ya and Yp was 12.8 %, which caused by uncontrollable factors; the yield gap between Ypf and Ya was 17.6 %, which caused by agronomic factors; and the yield gap between Yf and Ypf was 33.5 %, which caused by socioeconomic factors. During 1981-2017, climate, cultivar, sowing date and plant density change affected Ypf by -7.5, 4.5, -3.0 and - 2.0 %, respectively. By optimizing cultivar, sowing date and plant density, Ypf would increase by 13.1, 7.9 and 3.1 % and yield gap would close by 9.2, 5.6 and 2.1 %, respectively. By comprehensively optimizing cultivar, sowing date and plant density, Ypf would increase by 19.4 % and yield gap would close by 13.7 %. This work has practical significance for understanding climate, cultivar and agricultural management impacts on soybean yield, and demonstrates an effective approach, by optimizing cultivars and agricultural management practices to address climate change, increase yield and close yield gaps.

20% of uncertainty in yield estimates could be caused by the radiation source.

Solar radiation is the energy for all biological, physical, and chemical processes of the earth's surface system, and affects the growth and development of crops at all stages. But the diverse data sources and fusion algorithms lead to large differences in the radiation values in various climate datasets. Accurate estimates of the radiation data is not an easy task, the uncertainty of which and the impact on crop yield simulation remains unknown. In this study, the total solar radiation amounts from four independent global radiation datasets were shown considerable heterogeneity across regions and cropping seasons. Forcing the dynamic crop models with the four radiation inputs produced similarly great uncertainties of simulated yield in most regions, with the greatest uncertainty up to 30% of average yield for wheat in Europe. The global-scale uncertainty of simulated yield is increasing during the past three decades and would reach up to 20% of its averages in the future, equivalent to 300 million tons when converting to the global crop production. The results of this study suggest that the previously projected crop yield changes with climate change have large uncertainties propagated from solar radiation data sources used for projections. These uncertainties may mislead the assessment of future food security.

Impacts of mean climate and extreme climate indices on soybean yield and yield components in Northeast China.

Soybean is an important oil crop in China, and the national focus of soybean production is in Northeast China. In order to achieve high-stable yield, it is crucial to acknowledge the impacts of mean climate and extreme climate indices on soybean yield and yield components. In this study, based on the weather data from 61 counties from 1981 to 2017 in Northeast China, we assessed the impacts of mean climate and extreme climate indices on soybean observed yield and simulated yield. Mean climate include effective growing degree days (GDD10), precipitation (Pre), and solar radiation (SR); extreme climate indices include the number of cool days during seed-filling period (C15), the number of cool days during 15 days before anthesis (C17), the number of hot days (H30), maximum amount of 5 Day accumulated precipitation (P5), and consecutive dry days (CDD)). We used the DSSAT-CROPGRO-Soybean model to identify the main yield components for soybean. The results showed that observed soybean yield reduced by 3.57% due to the collective changes in the eight study climate indices. Increases in GDD10, decreases in Pre, and decreases in SR caused a 3.96%, -3.89%, and - 0.48% change in soybean yield, respectively. Decreases in C15 and C17 led to a 5.36% increase in soybean yield; increases in H30, P5, and CDD caused a 5.75%, 0.30%, and 1.14% reduction in soybean yield, respectively. By comparing the response of observed and simulated soybean yield to climate indices (excluding P5) in the DSSAT-CROPGRO-Soybean model, we identified the key yield components for soybean as the number of pods and seed weight. The negative impacts on the number of pods and seed weight were mainly attributed to changes in Pre and H30 from anthesis to podding and during seed-filling period. Our results could be used to assist the local soybean community adapt to climate change.

Radiation use efficiency and biomass production of maize under optimal growth conditions in Northeast China.

Accurate crop growth and radiation use efficiency (RUE) measurements under optimal growth conditions are required to predict grain yield near the genetic growth potential and estimate climate change effects on crop production. Research was conducted to quantify RUE of maize during the whole growth period, vegetative period (VP, phenological development stage DVS < 1.0), and reproductive period (RP, DVS ≥ 1.0) under the optimal growth condition in Northeast China. Maize was grown in three population density treatments (9.0, 7.5, and 6.0 plants m-2, referred to HD, MD, and LD respectively) over three growing seasons (2017-2019). The maximum Leaf Area Index (LAI) was observed after the anthesis period (DVS = 1.2), ranged from to 4.9 to 6.9, and then gradually decreases. Photosynthetically active radiation (PAR) intercepted by the canopy of maize was approximate 10% during the early stage of the vegetative period (DVS < 0.3). Then greatest differences occurred during the late vegetative period (DVS = 0.3 to 0.8). When DVS > 1.0, there is no significant difference between the three population density conditions. Estimates of RUE were obtained based on the intercepted PAR (IPAR) and absorbed PAR (APAR). RUE value was 3.97 g MJ-1 APAR or 3.73 g MJ-1 IPAR in the whole growth period, and did not vary among density treatments. Moreover, maximum RUE occurred during VP (4.11 g MJ-1 APAR) and declined during RP (3.95 g MJ-1 APAR). Therefore, we recommend that maize models that rely on RUE for aboveground biomass accumulation should be using a value of 3.97 g MJ-1 APAR for predicting optimum maize grain yield in Northeast China, especially for the potential yield.

Infant and preschooler feeding behaviors in Chinese families: A systematic review.

A systematic review was conducted on the literature on feeding behaviors in Chinese families of children under 6 years old. Forty relevant publications were identified, of which 33 were published in Chinese, 7 in English. All studies were questionnaire-based and used a cross-sectional research design. Approximately half of the studies reported a score for each feeding practice/style, based on a Likert scale; the other half dichotomized these scores into a percentage of the population that reported frequent use of the behaviors. The most commonly reported feeding style of Chinese caregivers was a locally defined "active response" style that somewhat resembled authoritative parenting. The most commonly reported feeding practices were praise, encouraging trying new foods, encouragement of balanced diet and encouragement of healthy eating. Some behaviors showed a great deal of variance in prevalence between studies, which may be at least partially due to differences in methodology and how behaviors were defined. Some feeding behaviors varied in frequency depending on the child's age, although longitudinal studies are needed to better understand how these evolve over time. Child body composition was also associated with feeding behaviors use, although the direction of the association cannot be determined due to the cross-sectional nature of the research. There is still an important gap in the literature regarding the feeding behaviors of non-maternal caregivers, as grandparents often play an important role in childcare in China.

Astragaloside IV Alleviates the Experimental DSS-Induced Colitis by Remodeling Macrophage Polarization Through STAT Signaling.

Inflammatory bowel disease (IBD) is characterized by chronic and relapsing intestinal inflammation, which currently lacks safe and effective medicine. Some previous studies indicated that Astragaloside IV (AS-IV), a natural saponin extracted from the traditional Chinese medicine herb Ligusticum chuanxiong, alleviates the experimental colitis symptoms in vitro and in vivo. However, the mechanism of AS-IV on IBD remains unclear. Accumulating evidence suggests that M2-polarized intestinal macrophages play a pivotal role in IBD progression. Here, we found that AS-IV attenuated clinical activity of DSS-induced colitis that mimics human IBD and resulted in the phenotypic transition of macrophages from immature pro-inflammatory macrophages to mature pro-resolving macrophages. In vitro, the phenotype changes of macrophages were observed by qRT-PCR after bone marrow-derived macrophages (BMDMs) were induced to M1/M2 and incubated with AS-IV, respectively. In addition, AS-IV was effective in inhibiting pro-inflammatory macrophages and promoting the pro-resolving macrophages to ameliorate experimental colitis via the regulation of the STAT signaling pathway. Hence, we propose that AS-IV can ameliorate experimental colitis partially by modulating macrophage phenotype by remodeling the STAT signaling, which seems to have an essential function in the ability of AS-IV to alleviate the pathological progress of IBD.

Noninvasive evaluation of HABP1 expression with 99mTc-labeled small-interference RNA in ovarian cancer.

PURPOSE: Ovarian cancer is one of the most common gynecological cancers in women with a low 5-year survival rate. Evaluation of hyaluronic acid-binding protein 1 (HABP1) level can provide important information for the diagnosis and treatment of ovarian cancer. In this study, we designed a novel HABP1 probe based on 99mTc-radiolabeled small-interference RNA (siRNA) for detecting HABP1 expression noninvasively in vivo, thereby providing a new method for its diagnosis and treatment. METHODS: A specific siHABP1 was selected because of its targetability and silencing effect. A negative control siRNA (NCsiRNA) with no homology with the human genome was used. SiHABP1 and NCsiRNA were radiolabeled with 99mTc using the bifunctional chelating agent hydrazinonicotinamide (HYNIC). The radiochemical purity and in vitro stability of the probe were determined by HPLC. The binding activity was measured by western blotting (WB) and RT-PCR. The HABP1-overexpressing human ovarian cancer cell line HO-8910 was used for cell uptake experiments, which were performed with or without transfection and measured with a gamma counter. HO8910-bearing mice were imaged at 1, 4, and 10 h, and biodistribution analysis was performed at 1, 4, 6, and 10 h after injection of 99mTc-HYNIC-siRNA. RESULTS: 99mTc-HYNIC-siHABP1 had high radiochemical purity and good in vitro stability, and showed the same binding capacity and silencing effect as siHABP1. SPECT imaging showed that tumors were clearly visualized at 10 h after injection of 99mTc-HYNIC-siHABP1 but not after 99mTc-HYNIC-NCsiRNA, implying specific binding. The biodistribution results were consistent with those of SPECT imaging. CONCLUSIONS: We showed that 99mTc-HYNIC-siHABP1 is a feasible probe for the noninvasive visualization of HABP1 expression in ovarian cancer.

[Notch signaling regulates M1-type polarization in macrophages by inhibiting signal regulatory protein α (SIRPα)].

Objective To investigate the role of Notch signaling in regulating the polarization of macrophages with signal regulatory protein α (SIRPα). Methods RAW264.7 cells were polarized into M1 phenotype after being treated with lipopolysaccharides (LPS) and interferon γ (IFN-γ) and into M2 phenotype with interleukin-4(IL-4). The mRNA levels of tumor necrosis factor α (TNF-α), IL-12, IL-10, mannose receptor (MR), and SIRPα were detected by real-time quantitative PCR. The protein expression level of SIRPα was detected by Western blotting. After Notch signal was activated by gene transfection or blocked by gamma-secretase inhibitor (GSI), SIRPα expression in macrophages was detected by Western blotting. SIRPα promoter region (-2615-+123) was amplified by mouse genomic DNA, and the regulatory effect of Notch activation on SIRPα was detected by reporter gene assay. Results The expression of SIRPα decreased in M1 type macrophages and increased in M2 type macrophages. Notch signaling inhibited the expression of SIRPα in macrophages, while GSI increased the expression of SIRPα in macrophages. Reporter gene assay confirmed that Notch activation significantly inhibited luciferase expression driven by SIRPα promoter fragment. Conclusion Notch signaling involves the M1-type polarization of macrophages by inhibiting the expression of SIRPα.

Comprehensive Analysis of GLUT1 Immune Infiltrates and ceRNA Network in Human Esophageal Carcinoma.

BACKGROUND: Glucose transporter 1 (GLUT1) is encoded by the solute carrier family 2A1 (SLC2A1) gene and is one of the glucose transporters with the greatest affinity for glucose. Abnormal expression of GLUT1 is associated with a variety of cancers. However, the biological role of GLUT1 in esophageal carcinoma (ESCA) remains to be determined. METHODS: We analyzed the expression of GLUT1 in pan-cancer and ESCA as well as clinicopathological analysis through multiple databases. Use R and STRING to perform GO/KEGG function enrichment and PPI analysis for GLUT1 co-expression. TIMER and CIBERSORT were used to analyze the relationship between GLUT1 expression and immune infiltration in ESCA. The TCGA ESCA cohort was used to analyze the relationship between GLUT1 expression and m6A modification in ESCA, and to construct a regulatory network in line with the ceRNA hypothesis. RESULTS: GLUT1 is highly expressed in a variety of tumors including ESCA, and is closely related to histological types and histological grade. GO/KEGG functional enrichment analysis revealed that GLUT1 is closely related to structural constituent of cytoskeleton, intermediate filament binding, cell-cell adheres junction, epidermis development, and P53 signaling pathway. PPI shows that GLUT1 is closely related to TP53, GIPC1 and INS, and these three proteins all play an important role in tumor proliferation. CIBERSORT analysis showed that GLUT1 expression is related to the infiltration of multiple immune cells. When GLUT1 is highly expressed, the number of memory B cells decreases. ESCA cohort analysis found that GLUT1 expression was related to 7 m6A modifier genes. Six possible crRNA networks in ESCA were constructed by correlation analysis, and all these ceRNA networks contained GLUT1. CONCLUSION: GLUT1 can be used as a biomarker for the diagnosis and treatment of ESCA, and is related to tumor immune infiltration, m6A modification and ceRNA network.

Oral health status among visually impaired schoolchildren in Northeast China.

BACKGROUND: Visual impairment is an important disability affecting a substantial proportion of people globally. The aim of this study was to assess the oral health status of visually impaired schoolchildren in northeast China, and to investigate the influencing factors. METHODS: The study was performed in 2015, according to the criteria and methods used in the Third National Oral Health Epidemiological Survey in China. One hundred and three visually impaired schoolchildren from the only special school for the blind in northeast China were included in the study. Oral examinations were performed to assess the caries of deciduous and permanent teeth, periodontal disease, malocclusion. A questionnaire-based survey was conducted to investigate oral health-related behaviors, knowledge and attitude about oral care. RESULTS: The overall prevalence of caries was 78.64%, and mean number of caries was 2.43 ± 2.75. The prevalence of caries in deciduous and permanent teeth was 65.22 and 71.84%, respectively. The rates of gingival bleeding and dental calculus were 44.66 and 67.96%, respectively. Malocclusion was observed in 49.51% of the children with visual impairment. The prevalence of caries was significantly higher in girls than boys (P < 0.05). The logistic regression analysis identified the knowledge level of parents and the toothache experience as risk factors for oral health, while the daily use of fluoride toothpaste could reduce the caries incidence. CONCLUSIONS: This group of visually impaired schoolchildren exhibited a high prevalence of dental caries, poor periodontal health, and severe malocclusion. Oral health status is relatively poor among visually impaired schoolchildren in northeast China. Factors that significantly affected the prevalence of dental caries included education level of the mother, experience of toothache, and use of fluoride toothpaste.