Shape variation and sex differences of the adult human mandible evaluated by geometric morphometrics.

In cases of osseous defects, knowledge of the anatomy, and its age and sex-related variations, is essential for reconstruction of normal morphology. Here, we aimed at creating a 3D atlas of the human mandible in an adult sample using dense landmarking and geometric morphometrics. We segmented 50 male and 50 female mandibular surfaces from CBCT images (age range: 18.9-73.7 years). Nine fixed landmarks and 510 sliding semilandmarks were digitized on the mandibular surface, and then slid by minimizing bending energy against the average shape. Principal component analysis extracted the main patterns of shape variation. Sexes were compared with permutation tests and allometry was assessed by regressing on the log of the centroid size. Almost 49 percent of shape variation was described by the first three principal components. Shape variation was related to width, height and length proportions, variation of the angle between ramus and corpus, height of the coronoid process and inclination of the symphysis. Significant sex differences were detected, both in size and shape. Males were larger than females, had a higher ramus, more pronounced gonial angle, larger inter-gonial width, and more distinct antegonial notch. Accuracy of sexing based on the first two principal components in form space was 91 percent. The degree of edentulism was weakly related to mandibular shape. Age effects were not significant. The resulting atlas provides a dense description of mandibular form that can be used clinically as a guide for planning surgical reconstruction.

A novel method for testing accuracy of bite registration using intraoral scanners.

Objective: The evidence on the accuracy of bite registration using intraoral scanners is sparse. This study aimed to develop a new method for evaluating bite registration accuracy using intraoral scanners. Methods: Two different types of models were used; 10 stone models and 10 with acrylic resin teeth. A triangular frame with cylindrical posts at each apex (one anterior and two posteriors) was digitally designed and manufactured using three-dimensional (3D) printing. Such a structure was fitted in the lingual space of each maxillary and mandibular model so that, in occlusion, the posts would contact their opposing counterparts, enforcing a small interocclusal gap between the two arches. This ensured no tooth interference and full contact between opposing posts. Bite registration accuracy was evaluated by measuring the distance between opposing posts, with small values indicating high-accuracy. Three intraoral scanners were used: Medit i500, Primescan, and Trios 4. Viewbox software was used to measure the distance between opposing posts and compute roll and pitch. Results: The average maximum error in interocclusal registration exceeded 50 µm. Roll and pitch orientation errors ranged above 0.1 degrees, implying an additional interocclusal error of around 40 µm or more. The models with acrylic teeth exhibited higher errors. Conclusions: A method that avoids the need for reference hardware and the imprecision of locating reference points on tooth surfaces, and offers simplicity in the assessment of bite registration with an intraoral scanner, was developed. These results suggest that intraoral scanners may exhibit clinically significant errors in reproducing the interocclusal relationships.

Covariance patterns between ramus morphology and the rest of the face: A geometric morphometric study.

Objective: The growth and development of the mandible strongly depend on modeling changes occurring at its ramus. Here, we investigated covariance patterns between the morphology of the ramus and the rest of the face. Methods: Lateral cephalograms of 159 adults (55 males and 104 females) with no history of orthodontic treatment were collected. Geometric morphometrics with sliding semi-landmarks was used. The covariance between the ramus and face was investigated using a two-block partial least squares analysis (PLS). Sexual dimorphism and allometry were also assessed. Results: Differences in the divergence of the face and anteroposterior relationship of the jaws accounted for 24.1% and 21.6% of shape variation in the sample, respectively. Shape variation was greater in the sagittal plane for males than for females (30.7% vs. 17.4%), whereas variation in the vertical plane was similar for both sexes (23.7% for males and 25.4% for females). Size-related allometric differences between the sexes accounted for the shape variation to a maximum of 6% regarding the face. Regarding the covariation between the shapes of the ramus and the rest of the face, wider and shorter rami were associated with a decreased lower anterior facial height as well as a prognathic mandible and maxilla (PLS 1, 45.5% of the covariance). Additionally, a more posteriorly inclined ramus in the lower region was correlated with a Class II pattern and flat mandibular plane. Conclusions: The width, height, and inclination of the ramus were correlated with facial shape changes in the vertical and sagittal planes.

In Vitro Trueness and Precision of Intraoral Scanners in a Four-Implant Complete-Arch Model.

(1) Background: New intraoral (IOS) and laboratory scanners appear in the market and their trueness and precision have not been compared. (2) Methods: Seven IOS and two laboratory scanners were used to scan a mandibular edentulous model with four parallel internal hexagon implant analogues and PEEK scan bodies. Digital models in Standard Tessellation Language (STL) were created. The master model with the scan bodies was scanned (×10) with a computerized numerical control 3D Coordinate Measuring Machine (CMM). The short (distances of adjacent scan posts) and long distances (distances of the scan posts with non-adjacent sites in the arch) among the centroids of the four analogues were calculated using CMM special software. Trueness (comparisons with the master model) and precision (intragroup comparisons) were statistically compared with ANOVA, chi-square and Tukey tests. (3) Results: Laboratory scanners had the best trueness and precision compared to all IOSs for long distances. Only iTero (Align Technologies Inc., Milpitas, CA, USA) had comparable trueness with one laboratory scanner in short and long distances. For short distances, CS3600 (Carestream Health, Inc., Rochester, NY, USA), Omnicam, Primescan (Sirona Dental Sys-tems GmbH, Bens-heim, Germany) and TRIOS 4 (3Shape A/S, Copen-hagen, Denmark) had similar trueness to one laboratory scanner. From those, only Omnicam and Primescan had similar precision as the same laboratory scanner. Most IOSs seem to work better for smaller distances and are less precise in cross-arch distances. (4) Conclusions: The laboratory scanners showed significantly higher trueness and precision than all IOSs tested for the long-distance group; for the short distance, some IOSs were not different in trueness and precision than the laboratory scanners.

Novel method to delineate palatal rugae and assess their complexity using fractal analysis.

Palatal rugae constitute significant morphological landmarks, with wide clinical applications in forensics, insertion of mini-screws, and superimposition. Their morphology has been studied mainly with indices relevant to their qualitative characteristics. The present paper aims at presenting a new quantitative method to evaluate their complexity, by means of box-counting fractal dimension analysis, and to investigate its inter- and intra-rater reliability. Twenty maxillary plaster models were scanned for the needs of this study. A sequence of steps, including cropping of the mesh, ball pivoting, distance mapping and fractal dimension analysis, performed with Viewbox 4 software, was followed. Box-counting fractal dimensions were calculated as a measure of rugae's complexity. Inter- and intra-rater reliability were investigated, using Bland-Altman analysis. Fractal dimensions ranged from 1.274 to 1.491 (average: 1.412). Bland-Altman analysis of inter- and intra-examiner reliability demonstrated that the 95% limits of agreement ranged from - 0.012 to 0.011 and from - 0.004 to 0.004, respectively. The method is reliable and can be applied in research and forensics. It offers comprehensive evaluation of the rugae's complexity and a complete set of information about their outlines and height profiles, with minimum user intervention.

The Effect of Scanning Strategy on Intraoral Scanner's Accuracy.

The purpose of the present study was to examine the impact of scanning strategy on trueness and precision of the impression acquired from an intraoral scanner. Fifteen complete-arch, mandibular, post-orthodontic treatment casts were scanned with a laboratory scanner (Identica SE 3D, Medit) as the gold standard, and with an intraoral scanner (i500 Medit) following three different paths of the scanning head over the arch (scanning strategies A, B, and C). The hand scans were performed twice by one examiner and repeated by a second examiner, resulting in 180 triangular mesh surfaces (digital casts). The meshes were superimposed on the gold standards using the Viewbox 4 software. The closest distances between the meshes were computed and trueness and precision were evaluated using a General Linear Model. An interaction was found among the examiner and strategy. The accuracy of complete-arch impressions was affected by the scanning strategy; the manufacturer's recommended strategy (A) was statistically significantly better (p < 0.05) than B and C, which were similar. An average accuracy below 50 µm, which is clinically acceptable in most orthodontic procedures, was achieved with all the examined scanning strategies.

Number of Teeth Is Related to Craniofacial Morphology in Humans.

One of the most common dental anomalies in humans is the congenital absence of teeth, referred to as tooth agenesis. The association of tooth agenesis to craniofacial morphology has been previously investigated but remains unclear. We investigated this association by applying geometric morphometric methods in a large sample of modern humans. In line with previous studies, we report here that a reduced teeth number is linked to a less convex profile, as well as to a shorter face. The effects were similar for males and females; they increased as the severity of the tooth agenesis increased and remained unaltered by the inclusion of third molars and of allometry in the analysis. Furthermore, in cases with tooth agenesis only in the maxilla, there was no detectable effect in mandibular shape, whereas maxillary shape was affected independently of the location of missing teeth. The robustness of the present sample along with the shape analysis and the statistical approach applied, allowed for thorough testing of various contributing factors regarding the presence but also the magnitude of effects. The present findings suggest a relationship between number of teeth and overall craniofacial development and have evolutionary implications.

Drug-induced sleep endoscopy improves intervention efficacy among patients treated for obstructive sleep apnea with a mandibular advancement device.

PURPOSE: To compare the short-term treatment effect of a mandibular advancement device (MAD) with and without previous drug-induced sleep endoscopy (DISE) on polysomnography (PSG) and other sleep apnea-related treatment outcomes (Short Form Health Survey [SF-36] and Epworth Sleepiness Scale [ESS]) among adults with mild, moderate, and severe obstructive sleep apnea (OSA). We hypothesized that using DISE would improve the efficacy of MADs on the sleep apnea parameters. METHODS: The study sample consisted of patients with OSA who were unable or unwilling to tolerate a CPAP device, divided into an experimental (with DISE) and a control (without DISE) group. RESULTS: Of 50 patients with OSA, 40 men (80%), mean age was 48.8 ± 12,3 years. The mean apnea-hypopnea index (AHI) score of both groups decreased significantly between baseline and the 8-week follow-up titration PSG with MAD in situ, from 31.7 ± 17.3 (mean ± SD) apnea-hypopnea episodes/h to 7.0 ± 6.4/h (p < 0.0001) in the experimental group, and from 22.5 ± 16.6 episodes/h to 11.4 ± 8.0/h (p < 0.024) in the control group. Capillary oxygen saturation (SpO2) levels did not change significantly between the two timepoints for either group. The SF-36 (p < 0.023) and ESS (p < 0.036) results of both groups improved significantly between baseline and the 8-week follow-up after starting MAD treatment; however, the improvement in quality of life was significantly more pronounced in the experimental group than in the control group (p < 0.0001). CONCLUSION: DISE provides a significant benefit to patients with OSA undergoing MAD treatment. It can be used as a valuable prediction tool in clinical practice for the management of patients with OSA, even those with moderate and severe disease.

Sociomedical and oral factors affecting masticatory performance in an older population.

OBJECTIVES: To assess the sociomedical and oral factors affecting masticatory performance in a community-dwelling older population. MATERIALS AND METHODS: Community-dwelling persons over 60 years were investigated using medical and dental oral interviews, oral and denture examination (natural teeth, tooth mobility, number of occluding tooth pairs, and removable dentures' prevalence and quality), and evaluation of masticatory performance using a mixing ability test. RESULTS: A total of 130 participants with a mean age of 73.9±8.5 years were recorded. Fifty-eight (44.6%) used various types of removable prostheses. Twenty were edentulous and used a pair of complete dentures. Univariate analyses revealed statistically significant associations (p≤0.05) between masticatory performance and aging, marital status, subjective chewing ability, use of removable dentures, use of various combinations of complete dentures, pain caused by maxillary denture, number of teeth, tooth mobility, posterior chewing pairs, all chewing contacts natural or prosthetic, retention of mandibular partial dentures, and dentures' occlusion. The multivariable quantile regression analysis revealed that fewer natural teeth (95% CI: -0.02-0.01, p<0.001), being edentulous and using a pair of complete dentures (95% CI: 0.09-0.35, p=0.001), and larger percentage of severely mobile teeth (95% CI: 0.07-0.82, p=0.020) were associated with lower masticatory performance. CONCLUSIONS: Poor masticatory performance in older adults was associated with fewer teeth, being edentulous and using a pair of complete dentures, and increased prevalence of severe tooth mobility. CLINICAL RELEVANCE: Retaining the natural dentition and preventing and treating periodontal disease are important measures to maintain masticatory performance in older adults.

A randomized, 3-month, parallel-group clinical trial to compare the efficacy of electric 3-dimensional toothbrushes vs manual toothbrushes in maintaining oral health in patients with fixed orthodontic appliances.

INTRODUCTION: The aim of this single-blinded, parallel-group, randomized clinical trial was to compare the efficacy of electric 3-dimensional (3D) toothbrushes and manual toothbrushes in removing plaque and reducing gingival inflammation in orthodontic patients. METHODS: Eighty adolescents with fixed orthodontic appliances in both arches were randomized at a 1:1 ratio, with an equal number of both sexes, in this examiner blinded, parallel clinical trial. Eligibility criteria included subjects aged between 12 and 16 years, good general health, nonextraction orthodontic treatment, and plaque-induced gingivitis, excluding patients with active caries or periodontitis, tooth agenesis, syndromes, disabilities, and craniofacial deformities, ≥2 cervical and/or proximal fillings, dental prostheses or dental implants, and subjects smoking or using antibiotics or medication predisposing to gingival enlargement. Patients were assigned to use either an electric 3D orthodontic toothbrush (Oral-B Pro-1000 with Oral-B Ortho head; Procter & Gamble, Cincinnati, Ohio) or a manual toothbrush (Oral-B Orthodontic brush; Procter & Gamble) and instructed to brush twice daily for 2 minutes. The main outcomes were: (1) plaque removal, assessed with the Modified Silness and Löe plaque index and the Modified Full Mouth Plaque Score, and (2) gingival inflammation reduction, assessed with the Modified Silness and Löe Gingival Index and the Modified Simplified Gingival Index. Measurements were taken at baseline, 1, 2, and 3 months. Randomization was achieved with 2 random sequences, one for each brush, written and sealed in opaque numbered envelopes. Blinding was possible for outcome assessment only. RESULTS: Considerable variability was observed among patients in the values of all dependent variables. There was no difference between interventions over time for any of the outcomes, and the main effects for treatment and time were also not statistically significant. For Modified Silness and Löe plaque index, the interaction was 0.001 (95% confidence interval, -0.011 to 0.013; P = 0.89). CONCLUSIONS: No difference in plaque removal efficacy and gingival inflammation reduction was found between the electric 3D and manual toothbrushes in adolescents with fixed orthodontic appliances. Therefore, orthodontists should focus on enhancing their patients' dental awareness and oral hygiene, along with professional prophylaxis and other oral hygiene aids, independently of the brush used. REGISTRATION: This trial was registered at ClinicalTrials.gov (Identifier: NCT02699931). PROTOCOL: The protocol was not published before trial commencement. FUNDING: Electric and manual toothbrushes and toothpastes for all participants were provided by Procter & Gamble (Oral-B). Miscellaneous costs were covered by the participating departments.

Oral factors and adherence to Mediterranean diet in an older Greek population.

PURPOSE: The aim of this study was to investigate the effect of oral factors on adherence to the Mediterranean diet in an older population METHODS: 130 persons over 60 years visiting Open Care Community Centers for Older People participated in this study. Oral interviews recorded demographic and sociomedical information, subjective oral complaints, and dental habits. Adherence to Mediterranean diet was assessed using the MDI_BNC4H index (range: 0-14). An oral examination was performed, and evaluation of the masticatory performance was carried out using a two-color chewing gum that was digitally analysed. RESULTS: The mean age of the study participants was 73.9 ± 8.5 years. The score of adherence to the Mediterranean diet ranged from 3 to 9 (5.6 ± 1.4). 58 participants used removable prostheses, while 20 used a pair of complete dentures. Univariate analyses revealed that the parameters that negatively significantly, or marginally significantly, affected the level of adherence to the Mediterranean diet were lower masticatory performance (p = 0.050), larger number of drugs per day (p = 0.056), higher BMI (p = 0.043) and smoking (p = 0.053). The multivariable analysis revealed that lower adherence to the Mediterranean diet was significantly associated with higher BMI (p = 0.047) and lower masticatory performance (p = 0.050). CONCLUSIONS: Increased masticatory performance was an independent predictor of better adherence to the Mediterranean diet in an older population.

Relapse 1 week after bracket removal: a 3D superimpositional analysis.

OBJECTIVES: To measure tooth movement 1 week post-treatment and assess potential correlation with changes invoked during treatment. SUBJECTS AND METHODS: Thirty-eight patients were recruited (19 males, 19 females). Polyvinyl siloxane impressions were taken after bracket debonding (T1) and 1 week later (T2) and digitally scanned. During this period no retention was used. The digital casts were superimposed on structures of the hard palate. Translation and rotation of the first molars, canines, and central incisors were recorded. Additionally, movement of these teeth was assessed from the beginning (T0) until the end of treatment (T1). The correlation between the post-treatment relapse (T1-T2) and tooth movement during treatment (T0-T1) was investigated via the Spearman correlation coefficient. RESULTS: Relapse was detected and reflected changes in tooth position during treatment. For the first molars (right, left) the correlation between treatment and post-treatment tooth movement was evident in the transverse direction (r = -0.38, P = 0.020; r = -0.32, P = 0.052), tipping (r = -0.40, P = 0.015; r = -0.34, P = 0.034) and the antero-posterior direction (r = -0.31, P = 0.061; r = -0.36, P = 0.027); for the canines (right and left), as rotation around their long axis (r = -0.55, P = 0.003; r = -0.58, P = 0.002); for central incisors (right and left) in the antero-posterior direction (r = -0.55, P = 0.000; r = -0.48, P = 0.03), transverse direction (r = -0.43, P = 0.07; r = -0.32, P = 0.047), and rotation around their long axis (r = -0.53, P = 0.001; r = -0.28, P = 0.089). CONCLUSIONS: Post-treatment changes in tooth position were mostly related to tooth movement during treatment. The reported correlations may help clinicians predict short-term relapse, evaluate long-term retention need, and design individualized retention schemes.

Number of teeth is associated with facial size in humans.

During human evolution there has been an increase in the size of the brain and the cranium, whereas the size of the face, as well as the size and number of teeth have decreased. In modern humans, the occurrence of missing permanent teeth, namely tooth agenesis, is common. It could be attributed to a biological mechanism of tooth number reduction that has evolved during time and might still be active. Although, if evident, it would add support to this theory, the relationship between this phenotype and craniofacial size remains largely unknown. The present case-control study shows that modern individuals with tooth agenesis have indeed smaller facial configurations. For example, a 15-year-old female with no, one, or ten missing teeth would have a facial centroid size of 511.83, 510.81, or 501.70 mm, respectively. No such effect was observable in the cranial base and the cranium. Our results suggest that common gene regulatory mechanisms that have evolved over time, continue to regulate the number of teeth and facial size of modern humans in a coordinated manner. We anticipate our findings to enrich our understanding of the evolution and development of the human head and kindle future developmental research on this field.

Estimation of root inclination of anterior teeth from virtual study models: accuracy of a commercial software.

BACKGROUND: The aim of the study was to assess the accuracy of commercially available software in estimating anterior tooth root inclination from digital impressions of the crowns of the teeth. SUBJECTS AND METHODS: Following sample size calculation and application of inclusion and exclusion criteria, 55 anterior natural teeth derived from 14 dry human skulls were selected. Impressions were taken and plaster study models were fabricated. Plaster models were scanned using the high-resolution mode of an Ortho Insight 3D laser scanner. The teeth on the digital scans were segmented and virtual roots were predicted and constructed by the Ortho Insight 3D software. The 55 natural teeth were removed from the dry skulls and scanned using the Identica extraoral white-light scanner in order to calculate their actual root angulation. The teeth were scanned twice, once to acquire the crown and the cervical part of the root, and a second time to acquire the remaining part of the root, including the apex. The two scanned segments were joined in software by superimposing them along their common part. The accuracy of the digital models generated by the Ortho Insight 3D scanner in predicting root angulation was assessed by comparing these results to the corresponding measurements of the 55 natural teeth. The long axes of the tooth models obtained from the software prediction and the scanning of the actual teeth were computed and the discrepancy between them was evaluated. The error of the methods was evaluated by repeating the measurements on 14 teeth and showed an acceptable range. RESULTS: The predicted tooth angulation was found to differ significantly from the actual angulation, both statistically and clinically. The angle between the predicted and actual long axes ranged from 2.0 to 37.6°(average 9.7°; median 7.4°). No statistically significant difference was found between tooth categories. CONCLUSIONS: Further investigations and improvements of the software are needed before it can be considered clinically effective.

A geometric morphometric evaluation of hard and soft tissue profile changes in borderline extraction versus non-extraction patients.

OBJECTIVES: To evaluate the hard tissue and facial profile changes in matched extraction and non-extraction Class I patients by the use of geometric morphometrics. SUBJECTS AND METHODS: From a parent sample of 542 Class I patients, previously subjected to discriminant analysis, a subsample of 68 borderline cases was obtained, 34 treated with extraction and 34 without extraction of 4 first premolars. Geometric morphometric methods (Procrustes superimposition and Principal Component Analysis) were applied on cephalometric tracings to assess the validity of the discriminant analysis in successfully identifying a morphologically homogeneous group and to evaluate inter- and intra-group skeletal and facial profile shape changes. RESULTS: No significant pre-treatment shape difference between the two groups was found, thus validating the discriminant analysis. The non-extraction group showed increase in hard tissue facial height (P < 0.001), with slight lower lip retrusion and upper lip protrusion (P = 0.027). The extraction group showed retraction of the hard tissue and facial profile outline (P < 0.001). Permutation tests for post-treatment inter-group differences resulted in P = 0.054 for the soft tissue outline and P = 0.078 for the hard tissue skeletal component. CONCLUSIONS: The evidence indicates that borderline cases treated with four premolar extractions will exhibit lip retrusion compared to non-extraction treatment.

Anterior teeth root inclination prediction derived from digital models: A comparative study of plaster study casts and CBCT images.

BACKGROUND: To assess the accuracy of digital models generated using commercially available software to predict anterior teeth root inclination characteristics and compare the results to relevant data obtained from CBCT images. MATERIAL AND METHODS: Following sample size calculation and after application of inclusion and exclusion criteria, pre-treatment maxillary and mandibular plaster models and the corresponding CBCT scans of 31 patients attending a private orthodontic clinic were selected. The subjects were 10 males and 21 females with age range 12 to 40 years. Plaster models were scanned using the high resolution mode of an Ortho Insight 3D scanner and CBCT scans were taken using a Kodak 9500 Cone Beam 3D System machine. The teeth on the digital scans were segmented and virtual roots were predicted and constructed by the Ortho Insight 3D software. The long axes of the predicted roots and the actual roots, as segmented from the CBCT images, were computed using best-fit lines. The inter-axis angle was used to assess error in root inclination prediction by the software. Mann-Whitney and Kruskal-Wallis tests were used. Intra-examiner error was evaluated using the Bland-Altman method. RESULTS: The maximum disparity in angle between images derived from digital models and CBCT data was almost 40 degrees (upper left canine). The upper and lower canines produced the worst results, followed by the lower lateral incisors. The upper central incisors showed the best results, although the maximum angle of difference exceeded 20 degrees (with the median around 8 degrees). CONCLUSIONS: Root morphology imaging prediction is not a primary function of this software and this study confirmed its limitation as a sole tool in routine clinical applications. At present these predictions cannot be considered accurate or reliable unless correlated clinically with a radiographic image. Key words:Digital models, CBCT, tooth root inclination prediction software.

Morphometric covariation between palatal shape and skeletal pattern in Class II growing subjects.

OBJECTIVES: To evaluate the patterns of covariation between palatal and craniofacial morphology in Class II subjects in the early mixed dentition by means of geometric morphometrics. METHODS: A cross-sectional sample of 85 Class II subjects (44 females, 41 males; mean age 8.7 years ± 0.8) was collected retrospectively according to the following inclusion criteria: European ancestry (white), Class II skeletal relationship, Class II division 1 dental relationship, early mixed dentition, and prepubertal skeletal maturation. Pre-treatment digital 3D maxillary dental casts and lateral cephalograms were available. Landmarks and semilandmarks were digitized (239 on the palate and 121 on the cephalogram) and geometric morphometric methods (GMM) were applied. Procrustes analysis and principal component analysis (PCA) were performed to reveal the main patterns of palatal shape and craniofacial skeletal shape variation. Two-block partial least squares analysis (PLS) assessed patterns of covariation between palatal morphology and craniofacial morphology. RESULTS: For the morphology of the palate, the first principal component (PC1) described variation in all three dimensions. For the morphology of the craniofacial complex, PC1 showed shape variation mainly in the vertical direction. Palatal shape and craniofacial shape covaried significantly (RV coefficient: 0.199). PLS1 accounted for more than 64 per cent of total covariation and related divergence of the craniofacial complex to palatal height and width. The more a Class II subject tended towards high-angle divergence, the narrower and higher was the palate. CONCLUSIONS: Class II high-angle patients tended to have narrower and higher palates, while Class II low-angle patients were related to wider and more shallow palates.

Craniofacial shape differs in patients with tooth agenesis: geometric morphometric analysis.

AIM: To evaluate the shape of the craniofacial complex in patients with tooth agenesis and compare it to matched controls. SUBJECTS AND METHODS: The sample comprised 456 patients that were allocated to three groups: the agenesis group of 100 patients with at least one missing tooth, excluding third molars, the third molar agenesis group (3dMAG; one to four missing third molars) of 52 patients and the control group (CG) of 304 patients with no missing teeth. The main craniofacial structures depicted on lateral cephalograms were digitized and traced with 15 curves and 127 landmarks. These landmarks were subjected to Procrustes superimposition and principal component analysis in order to describe shape variability of the cranial base, maxilla and mandible, as well as of the whole craniofacial complex. For statistical analysis, permutation tests were used (10 000 permutations without replacement). RESULTS: Approximately half of the sample's variability was described by the first three principal components. Comparisons within the whole sample revealed sexual dimorphism of the craniofacial complex and its structures (P < 0.01). Differences between the agenesis group and matched controls were found in the shape of all craniofacial structures except for the cranial base (P < 0.05). Specifically, patients with agenesis presented with Class III tendency and hypodivergent skeletal pattern. However, the comparison between the 3dMAG and matched CG revealed no differences. CONCLUSION: The shape of the craniofacial complex differs in patients with tooth agenesis suggesting that common factors are implicated in tooth development and craniofacial morphology.