α-Fe2 O3 @Ag and Fe3 O4 @Ag Core-Shell Nanoparticles: Green Synthesis, Magnetic Properties and Cytotoxic Performance.

This work provides the synthetic route for the arrangement of Fe3 O4 @Ag and α-Fe2 O3 @Ag core-shell nanoparticles (NPs) with cytotoxic capabilities. The production of Fe3 O4 @Ag and α-Fe2 O3 @Ag core-shell NPs was facilitated utilizing S. persica bark extracts. The results of Powder X-ray Diffraction (PXRD), Ultraviolet-visible (UV-Vis) spectroscopy, Vibrating Sample Magnetometry (VSM), Energy Dispersive X-ray (EDX) analysis, Field Emission Scanning Electron Microscopy (FESEM), and Transmission Electron Microscopy (TEM) supported the green synthesis and characterization of Fe3 O4 @Ag and α-Fe2 O3 @Ag NPs. The particle size was measured by the TEM analysis to be about 30 and 50 nm, respectively; while the results of FESEM showed that α-Fe2 O3 @Ag and Fe3 O4 @Ag particles contained multifaceted particles with a size of 50-60 nm and 20-25 nm, respectively. The outcomes of VSM were indicative of a saturation magnetization of 37 and 0.18 emu/g at room temperature, respectively. The potential cytotoxicity of the synthesized core-shell nanoparticles towards breast cancer (MCF-7) and human umbilical vein endothelial (HUVEC) cells was evaluated by an MTT assay. α-Fe2 O3 @Ag NPs were able to destroy 100 % of MCF-7 cell at doses above 80 µg/mL, and it was confirmed that Fe3 O4 @Ag NPs at a volume of 160 µg/mL can destroy 90 % of MCF-7 cells. Thus, the applicability of the prepared nanoparticles of these nanoparticles in biological and medical fields has been demonstrated.

Evaluation of cytotoxicity, loading, and release activity of paclitaxel loaded-porphyrin based metal-organic framework (PCN-600).

Considering the inducement side impacts and precipitation of continual doses in conventional therapeutic treatments, there is an urgent need in the field of drug delivery for novel designs of biocompatible carriers with wide loading dimensions and particularly the ability to control their drug release. In this work, we succeeded in synthesizing an iron-based organic metal framework based on iron-porphyrin (PCN-600) through a solvothermal method to function as a drug delivery system (DDS). According to SEM results, PCN-600 crystals a hexagonal-rod shaped morphology with the length of 300 nm and width of 100-300 nm. As an anticancer drug, Paclitaxel (PTX) was successfully loaded into the porphyrin-based metal-organic framework (PCN-600) via in-situ encapsulation; the loading efficiency was measured to be about 87.3%. In addition, PTX-encapsulated PCN-600 displayed a controlled and sustained release for up to 24 h of release assessment at the physiological microenvironment of pH = 7.4.

Study of photocatalytic activity of green synthesized nickel oxide nanoparticles in the degradation of acid orange 7 dye under visible light.

Environmental pollution is one of the most important problems that human beings face. Today, nanotechnology has played an important role in green chemistry and the use of nanoparticles in the removal of environmental pollutants is one of the newest methods of removing pollutants in the world. So, in this study, Nickel oxide nanoparticles (NiO NPs) of this work were successfully synthesized via a green method by the usage of nickel nitrate hexahydrate as the source of metal and Biebersteinia multifida extract as the stabilizing agent throughout different annealing temperatures. The physicochemical properties of the obtained NiO NPs were characterized through the application of scanning electron microscopy (SEM), energy dispersive X-ray (EDX), powder X-ray diffraction (PXRD), ultraviolet visible (UV-vis), and Raman analysis. According to the results of SEM and PXRD, the prepared product contained a satisfying distribution and very fine cubic structure with minimal accumulation. The average crystal size of prepared nanoparticles was obtained 54-58 nm. The energy band gap of synthesized NiO NPs was calculated 3-3.7 using Tauc equation. The photocatalytic performance of NiO NPs was investigated under visible light through the decolourization reaction of acid orange 7 (AO7) dye in aqueous solution. Being composed at 300 °C of annealing temperature, these nanoparticles exhibited excellent adsorption and photocatalytic activity (90.2%) toward AO7 dye. Therefore, it can be indicated that the synthesized NiO NPs demonstrated an excellent dispersion in dye solution, as well as considerable photocatalytic activity.

Consistency evaluation between matrix components ratio and microbiological potency of tylosin major components.

BACKGROUND: The aim of our research work was to investigate the relative potencies of matrix components of tylosin, a multi-component antibiotic, and establishing a quantitative relationship between content and potency of each component. METHODS: The potencies of tylosin matrix components were determined by using three bioassay methods. The content of tylosin components (tylosin A, B, C, and D) in different tylosin samples were determined by using high pressure liquid chromatography (HPLC) technique and their theoretical potencies were calculated. Equivalency of theoretical and microbiological potencies for each sample was evaluated using statistical analysis. RESULTS: The highest amount of tylosin B content was found in tylosin phosphate and tartrate (up to 19%). Tylosin D content in all tylosin samples varied in the range of 0.03 to 18.73%. Tylosin A, B, and C showed similar sensitivity to the Kocuria rhizophila, the test organism in agar-diffusion method, while the potency of tylosin D was 39% of A. In the turbidimetric methods by Staphylococcus aureus, tylosin D and B responses to A component were ranged from 22.5 to 22.8% and 77.3 to 79.3%, respectively, while potencies of tylosin C and A were almost equal. The biopotency conversion factors were not resulted to a single factor, due to the different antibacterial activity of tylosin components. CONCLUSION: Our findings indicated that defining individual limit for the low active matrix components and for the total of other components with similar high activity could improve the accuracy of potency results. Graphical abstract ᅟ.